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Search results for: EIA, FIA, ELISA
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PCR-ELISA: inexpensive alternative to quantitative PCR
PublicationPCR-ELISA (polymerase chain reaction-enzyme linked immunosorbent assay), a combination of PCR and ELISA methods, has been used since late 1980s. The technique is based on specially labelled DNA fragments which are captured by specific DNA sequences and detected by antibodies. The whole procedure of PCR-ELISA is divided into three steps: DNA extraction, PCR reaction and detection by ELISA. The method has been found as very specific...
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EIA in teaching sustainable development and environmental protection in engineering education
PublicationA multifaceted approach in teaching the environmental impact assessment (EIA) as a way to stress the role of environmental education in technical sciences is the focus of this article. The EIA is an example of an effective tool that supports spatial planning in implementing sustainable development. The aim is to present the idea and benefits of a facilitative and collaborative approach in teaching the EIA, stressing the role of...
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Landscape, EIA and decision-making. A case study of the Vistula Spit Canal, Poland
PublicationAlthough landscapes are often considered public goods, they frequently receive inadequate attention in Environmental Impact Assessments (EIAs), particularly in Poland. This neglect often leads to visible degradation during investment processes. This article examines the case of the Vistula Spit Canal, currently the largest engineering project under construction in Poland. We analysed whether the conclusions drawn in the EIA report,...
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The development of an indirect ELISA for the detection of goose parvovirus antibodies using specific VP3 subunits as the coating antigen
PublicationIn Poland, the leader in goose production in Europe, goose parovirus infection, or Derzsy’s disease (DD), must be reported to the veterinary administration due to the serious economic and epizootic threat to waterfowl production. Prophylactic treatment for DD includes attenuated live or inactivated vaccines. Moreover, the control of DD includes the monitoring of maternal derived antibody (MDA) levels in the offspring and antibody...
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A novel chemiluminescent immunoassay based on original acridinium ester labels as better solution for diagnosis of human toxoplasmosis than conventional ELISA test
PublicationToxoplasma gondii infection is one of the most common human zoonosis. Laboratory diagnosis of this disease is mainly based on the results of serological methods detecting specific antibodies in the patient’s sera. In this study we aimed to evaluate the performance of a chemiluminescence immunoassay (CLIA) based on the use of a novel immunochemical reagent in the form of the conjugate of original acridinium label (AL) attached to...
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WRS EiA
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Quantification of Radiation-Induced Single-Strand Breaks in Plasmid DNA using a TUNEL/ELISA-Based Assay
PublicationWprowadzono nową metodę służącą do oznaczania in vitro pojedynczych pęknięć nici DNA powstałych pod wpływem naświetlań. Porównano wyniki otrzymane z nowej metody z modelem teoretycznym. Porównano wyniki z szeroko używaną metodą elektroforezy żelowej. Pokazano, że poziom pojedynczych pęknięć powstających w niciach DNA pod wpływem promieniowania VUV jest wyższy niż zakładany na podstawie wyników otrzymywanych metodami standardowymi.
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Electrophoretic co-deposition of Mn1.5Co1.5O4, Fe2O3 and CuO: Unravelling the effect of simultaneous addition of Cu and Fe on the microstructural, thermo-mechanical and corrosion properties of in-situ modified spinel coatings for solid oxide cell interconnects
PublicationA systematic microstructural, thermo-mechanical and electrical characterization of simultaneous Fe–Cu doped Mn–Co spinel coatings processed by electrophoretic co-deposition on Crofer 22 APU is here reported and discussed. An innovative approach for the simultaneous electrophoretic deposition of three spinel precursors is designed, conceived and optimised, with the aim of outlining time- and energy-saving spinel modification routes....
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Toxoplasma gondii: Enzyme-Linked Immunosorbent Assay using different fragments of recombinant microneme protein 1 (MIC1) for detection of immunoglobulin G antibodies
PublicationTrzy rekombinantowe fragmenty białka antygenowego MIC1 Toxoplasma gondii (r-MIC1ex2, r-MIC1ex34 i r-MIC1) zostały wyprodukowane jako białka fuzyjne (zawierające dwie domeny oligohistydynowe na N- i C-końcu) w komórkach bakteryjnych Escherichia coli. Homogenne preparaty antygenów rekombinantowych otrzymane z zastosowaniem jednoetapowego oczyszczania metodą chromatografii metalopowinowactwa, wykorzystano następnie do immunoidentyfikacji...
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Iron doped manganese cobaltite spinel coatings produced by electrophoretic co-deposition on interconnects for solid oxide cells: Microstructural and electrical characterization
PublicationWe report a systematic microstructural and electrical characterization of iron doped Mn–Co spinel coatings processed by electrophoretic co-deposition of Mn1.5Co1.5O4 and Fe2O3 powders on Crofer 22 APU and AISI 441 steel substrates. Iron addition to Mn–Co spinel coating leads to a reduction of the area specific resistance on both substrates, after 3200 h at 750 °C. The Fe doped Mn–Co coating both leads to a thinner oxide scale and...
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Multilayer coatings based on cerium oxide and manganese cobaltite spinel for Crofer22APU SOC interconnects
PublicationThe current state of the art steel interconnect coating materials are based on critical raw material - Co-oxide spinels. Replacing Co-oxide spinels with alternative, abundant materials can reduce the dependence on the critical raw materials. Cobalt-free coatings with the general formula Mn2-xCuFexO4, where x = 0, 0.1, 0.3, were electrophoretically deposited on a ferritic stainless-steel support and evaluated. Prior to deposition,...
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Manganese–Cobalt Based Spinel Coatings Processed by Electrophoretic Deposition Method: The Influence of Sintering on Degradation Issues of Solid Oxide Cell Oxygen Electrodes at 750 °C
PublicationThis paper seeks to examine how the Mn–Co spinel interconnect coating microstructure can influence Cr contamination in an oxygen electrode of intermediate temperature solid oxide cells, at an operating temperature of 750 °C. A Mn–Co spinel coating is processed on Crofer 22 APU substrates by electrophoretic deposition, and subsequently sintered, following both the one-step and two-step sintering, in order to obtain significantly...
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Placement Elektrotechnika - 2 sem II st (EiA)
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A. Kucharska-Raczunas,EiA,Ist,2sem,23/24L
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A. Kucharska-Raczunas, EiA, Ist,IIIsem, 23/24Z
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A. Kucharska-Raczunas, EiA, Ist, Vsem, 23/24Z
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Anna Kucharska – Raczunas, EiA, Ist 2sem, 22/23L
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Ewa Rogala, EiA, ET , st.II, sem. 3, 20/21l
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Jolanta Wielgus, EiA, ACRiSS, Ist., 5 sem., 21/22z
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Novel monovalent and multivalent recombinant proteins of Borrelia burgdorferi sensu lato with potential diagnostic value – construction and biotechnological production
PublicationThe diverse antigenic structure of Borrelia burgdorferi sensu lato (s.l.) and the low degree of protein sequence conservation between genospecies causes many limitations in serodiagnosis of Lyme disease (LD). Using expression systems based on Escherichia coli, five monovalent B. burgdorferi s.l. recombinant proteins were produced. i.e., BB0108, BB0126, BB0298, BB0323, BB0689 (each in three variants derived from Borrelia afzelii,...
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Ewa Rogala, EiA, ET, II st, 3 sem, 21/22L
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Marek Adamczyk, EiA( ET), II st, 2 sem, 21/22z
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Alicja Dereniowska, EiA, AR, II st, 2 sem, 22/23z
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Jolanta Maciejewska EiA(AR) I st , 2 sem 21/22L
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Krzysztof Lis, EiA, Elektrotechnika II st. 1 sem. 20/21l
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Jolanta Maciejewska EiA(AR) I st , 3 sem 22/23Z
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Oksana Bielikowa, WEiA, EiA, I st, 3 sem, 23/24z
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Alicja Dereniowska, WEiA, EiA, I st, 3 sem, 23/24z
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Konrad Radomyski, WETI, EiA, I st, 4 sem, 23/24L
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Konrad Radomyski, WETI, EiA, I st, 3 sem, 23/24z
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Oksana Bielikowa, WEiA, EiA, I st, 2 sem, 23/24l
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Landscape protection - the challenge for sustainable planning
PublicationGrowing spatial chaos reminds us of the need for systematic, complex approaches related to environmental and landscape issues within different planning, organizational, operational, legal and political activities. It has been proved, that LVIA within EIA plays an important role in the enhancement of the spatial planning system, and that there is a need to use the potential of EIA/LVIA in spatial development and management. But...
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Jolanta Maciejewska EiA(AR) I st , 4 sem 22/23 L
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Barbara Ozimek EiA, AR II st., 2.sem.,20/21 l
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Alicja Dereniowska, WIMiO / EiA, Energetyka, I st, 2 sem, 22/23l
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Alicja Dereniowska, WIMiO / EiA, Energetyka, I st, 4 sem, 22/23l
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Hanna Rembowska, WIMIO / EIA, Energetyka, 1st, 2 sem, 2022/2023 l
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J.Pawlak-Mikuć 2, EiA(ET) I st, sem 4, 23/24
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Alicja Dereniowska, WIMiO / EiA, Energetyka, I st, 1 sem, 23/24z
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Alicja Dereniowska, WIMiO / EiA, Energetyka, I st, 4 sem, 23/24l
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Alicja Dereniowska, WIMiO / EiA, Energetyka, I st, 3 sem, 23/24z
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Rospuda - czy wszędzie budować mosty?
PublicationStreszczenie - SummaryThe article presents the problem of planning transport system in the areas of high natural and landscape values. Bridges and roads crossing valuable areas undergo Environmental Impact Assessment (EIA) Procedure. This procedure in Poland is often treated only as formal requirement, not as an instrument helping to solve conflicts and to minimise negative environmental and landscape effects. The potential of...
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Metoda wektorowa pomiaru impedancji pętli zwarciowej w obecności załóceń.** 2002, 116 s. 44 rys. 3 tab. bibliogr. 122 poz. maszyn. Rozprawa doktorska (2002.05.07), Wydz. EiA, P. Gdań. Promotor: dr hab. inż. R. Roskosz, prof. nadzw. PG.
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Krzysztof Lis, WIMiO, EiA, Energy Technologies, I st. 2 sem. 20/21l
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Małgorzata Piechocińska WIMIO/EiA Energetyka I st. 2 sem. 22/23 l
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Svitlana Radetka / Alicja Dereniowska, WEiA, EiA, I st, II sem, 22/23let
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J.Pawlak-Mikuć, EiA, Automatyka, Robotyka i SS, I st, 4 sem, 21/22l
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J.Pawlak-Mikuć, EiA, Automatyka, Robotyka i SS, I st, 3 sem, 21/22z
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Martyna Michalska-Pieniak, WEIA, EiA, 1 st, 4 sem, C1, 2023/2024 L
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Krzysztof Lis, OiO, WM, EiA, Energy Technologies, I st. 1 sem. 20/21z
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