Rok 2019

• PCR-RFLP assays for species-specific identification of fungi belonging to Scopulariopsis and related genera

  Publikacja

  • M. Kordalewska
  • J. Kalita
  • Z. Bakuła
  • A. Brillowska-Dąbrowska
  • T. Jagielski

  - MEDICAL MYCOLOGY - Rok 2019

  Fungi of the Scopulariopsis genus, commonly found in the environment, are opportunistic pathogens that can cause various types of human infections. So far, no efficient molecular method has been developed for species differentiation among Scopulariopsis and related genera. In order to advance this field, we have evaluated performance of polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) assays, based...

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Rok 2018

• Detection of Polish clinical Aspergillus fumigatus isolates resistant to triazoles

  Publikacja

  • U. Nawrot
  • E. Kurzyk
  • M. C. Arendrup
  • M. Mroczyńska-Szeląg (dawniej: M. Mroczyńska)
  • K. Włodarczyk
  • B. Sulik-tyszka
  • M. Wróblewska
  • M. Ussowicz
  • P. Zdziarski
  • K. Niewińska
  • A. Brillowska-Dąbrowska

  - MEDICAL MYCOLOGY - Rok 2018

  We studied the presence of triazole resistance of 121 Aspergillus fumigatus clinical isolates collected in two Polish cities, Warsaw and Wrocław, to determine if resistance is emerging in our country. We identified five itraconazole resistant isolates (4.13%) carrying the TR34/L98H alteration in Cyp51A gene, four of which were cross-resistant to posaconazole and one to voriconazole. One isolate was intermediate susceptible to itraconazole...

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Rok 2013

• PCR test for Microsporum canis identification

  Publikacja

  • A. Brillowska-Dąbrowska
  • E. Michałek
  • D. M. Saunte
  • S. S. Nielsen
  • M. C. Arendrup

  - MEDICAL MYCOLOGY - Rok 2013

  Microsporum canis, for which the natural hosts are cats and dogs, is the most prevalent zoophilic agent causing tinea capitis and tinea corporis in humans. We present here a diagnostic PCR test for M. canis, since its detection and species identification is relevant to the choice of treatment and to the understanding of a probable source of infection. An M. canis-specific PCR was evaluated using 130 clinical isolates of dermatophytes...

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Rok 2010

• Diagnostic PCR tests for Microsporum audouinii, M. canis and Trichophyton infections

  Publikacja

  • A. Brillowska-Dąbrowska
  • A. Świerkowska
  • D. M. Saunte
  • M. C. Arendrup

  - MEDICAL MYCOLOGY - Rok 2010

  Since traditional diagnosis of dermatophyte infections is slow, we present a rapid new pcr test for detection of trichophyton spp., microsporum canis and m. audouinii infections. the performance of the test was evaluated with: 58 dermatophyte isolates; 10 yeast, mould and human dna control samples; 25 routine specimens from patients suspected of having dermatophytosis; 10 hair specimens from guinea pigs experimentally infected...

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• Optimized 5-hour multiplex PCR test for the detection of tinea unguium: performance in a routine PCR laboratory

  Publikacja

  • A. Brillowska-Dabrowska
  • S. S. Nielsen
  • H. V. Nielsen
  • M. C. Arendrup

  - MEDICAL MYCOLOGY - Rok 2010

  We recently reported the development of a 5-hour multiplex PCR test for the detection of tinea unguium and the optimization of this test by the inclusion of an inhibition control. Here we report the performance of this procedure as used in a routine clinical laboratory as compared to conventional microscopy and culture-based techniques performed in a mycology reference laboratory. We found in processing 109 samples that 22 (20.2%)...

Rok 2008

• Experimental guinea pig model of dermatophytosis: a simple and useful tool for the evaluation of new diagnostics and antifungals

  Publikacja

  • D. M. Saunte
  • J. P. Hasselby
  • A. Brillowska-Dąbrowska
  • N. Frimodt-Møller
  • E. L. Svejgaard
  • . Linnemann D
  • S. S. Nielsen
  • M. Hædersdal
  • M. C. Arendrup

  - MEDICAL MYCOLOGY - Rok 2008

  Celem badań było opracowanie prostego modelu zwierzęcego (świnki morskie) odpowiedniego do sprawdzania systemów diagnostycznych oraz terapii infekcji dermatofitów. Następujące zmienne były analizowane: przygotowanie skóry przez golenie versus użycie taśmy, testowy szczep Microsporum canis czy Trichophyton mentagrophytes jako czynnik etiologiczny, sposób inokulacji. Przebieg infekcji był oceniany poprzez ocenę zaczerwienienia i...