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Construction, production and evaluation of the diagnostic utility of a recombinant Toxoplasma gondii chimeric antigen SAG1-SAG2-MAG1S
Opis
The intracellular parasite Toxoplasma gondii has the ability to infect a wide range of warm-blooded animals, including humans. Currently, diagnosis of toxoplasmosis is based mainly on the use of the native antigens in enzyme immunoassay which allow for detection of IgG, IgM and IgA antibody classes. However, in some cases the performed studies give the ambiguous results. Moreover, the commercially available diagnostic tests due to its price are not generally applicable in the serodiagnosis of toxoplasmosis in animals, which requires the analysis of a large number of samples. For this reason, many research groups are currently working on new diagnostic tools, which are mainly recombinant antigens. Compared to the native antigens their production is much easier, cheaper, faster and safer. An additional advantage of the recombinant antigens is easier way to standardize assays.
This study presents the construction of an efficient Escherichia coli expression system for the production of recombinant chimeric protein composed of the immunodominant regions of three Toxoplasma gondii antigens SAG1, SAG2 and MAG1. The produced and purified protein is composed of amino acid residues from: 49-311 of the SAG1 antigen, 30-170 of the SAG2 antigen, 30-222 of the MAG1 antigen (the immunodominant fragment well recognized by antibodies in the early stage of parasite invasion). In the next part of the work the diagnostic usefulness of obtaining proteins for detection of anti-T. gondii antibodies were evaluated in the IgG ELISA assay and agglutination test using animal sera (e.g. horses, ovine, and pigs) and human serum samples.
Plik z danymi badawczymi
hexmd5(md5(part1)+md5(part2)+...)-{parts_count} gdzie pojedyncza część pliku jest wielkości 512 MBPrzykładowy skrypt do wyliczenia:
https://github.com/antespi/s3md5
Informacje szczegółowe o pliku
- Licencja:
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Restricted access
- Oprogramowanie:
- SnapGene Viewer
Informacje szczegółowe
- Rok publikacji:
- 2021
- Data zatwierdzenia:
- 2021-05-28
- Data wytworzenia:
- 2021
- Język danych badawczych:
- angielski
- Dyscypliny:
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- nauki biologiczne (Dziedzina nauk ścisłych i przyrodniczych)
- nauki o zdrowiu (Dziedzina nauk medycznych i nauk o zdrowiu)
- Weterynaria (Dziedzina nauk rolniczych)
- DOI:
- Identyfikator DOI 10.34808/yg47-zd34 otwiera się w nowej karcie
- Finansowanie:
- Ethical papers:
- The sera were collected as part of the project entitled "Toxoplasmosis—facts and myths. Educational initiative raising social awareness about the infection with protozoan Toxoplasma gondii", foundation "Our Children", funded from the Civil Initiatives Fund of the Ministry of Labor and Social Policy (FIO 2008, contract No. 813).
- Weryfikacja:
- Politechnika Gdańska
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Wersja ten dokument posiada różne wersje
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Aktualna wersjawersja 1.1Data publikacji wersji 2021-05-28
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wersja 1.0Data publikacji wersji 2021-03-31
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