Construction, production and evaluation of the diagnostic utility of a recombinant Toxoplasma gondii chimeric antigen SAG1-SAG2-ROP1 - Open Research Data - MOST Wiedzy

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Construction, production and evaluation of the diagnostic utility of a recombinant Toxoplasma gondii chimeric antigen SAG1-SAG2-ROP1

Opis

The intracellular parasite Toxoplasma gondii has the ability to infect a wide range of warm-blooded animals, including humans. Currently, diagnosis of toxoplasmosis is based mainly on the use of the native antigens in enzyme immunoassay which allow for detection of IgG, IgM and IgA antibody classes. However, in some cases the performed studies give the ambiguous results. Moreover, the commercially available diagnostic tests due to its price are not generally applicable in the serodiagnosis of toxoplasmosis in animals, which requires the analysis of a large number of samples. For this reason, many research groups are currently working on new diagnostic tools, which are mainly recombinant antigens. Compared to the native antigens their production is much easier, cheaper, faster and safer. An additional advantage of the recombinant antigens is easier way to standardize assays.
This study presents the construction of an efficient Escherichia coli expression system for the production of recombinant chimeric protein composed of the immunodominant regions of three Toxoplasma gondii antigens SAG1, SAG2 and ROP1. The produced and purified protein is composed of amino acid residues from: 49-311 of the SAG1 antigen, 30-170 of the SAG2 antigen, 85-396 of the ROP1 antigen. In the next part of the work the diagnostic usefulness of obtaining proteins for detection of anti-T. gondii antibodies were evaluated in the IgG ELISA assay and agglutination test using animal sera (e.g. horses, ovine, and pigs) and human serum samples.

Plik z danymi badawczymi

pET30 SAG1-SAG2-ROP1.dna
76.7 kB, S3 ETag e3c9b1f20744c53994e31fcebb8c15eb-1, pobrań: 7
Hash pliku liczony jest ze wzoru
hexmd5(md5(part1)+md5(part2)+...)-{parts_count} gdzie pojedyncza część pliku jest wielkości 512 MB

Przykładowy skrypt do wyliczenia:
https://github.com/antespi/s3md5

Informacje szczegółowe o pliku

Licencja:
Restricted access
This dataset is a part of the patent application. If you are interested, please contact me (bartlomiej.ferra@pg.edu.pl).
Oprogramowanie:
SnapGene Viewer

Informacje szczegółowe

Rok publikacji:
2021
Data zatwierdzenia:
2021-03-31
Data wytworzenia:
2021
Język danych badawczych:
angielski
Dyscypliny:
  • nauki biologiczne (Dziedzina nauk ścisłych i przyrodniczych)
  • nauki o zdrowiu (Dziedzina nauk medycznych i nauk o zdrowiu)
  • Weterynaria (Dziedzina nauk rolniczych)
DOI:
Identyfikator DOI 10.34808/cazs-t550 otwiera się w nowej karcie
Finansowanie:
Ethical papers:
The sera were collected as part of the project entitled "Toxoplasmosis—facts and myths. Educational initiative raising social awareness about the infection with protozoan Toxoplasma gondii", foundation "Our Children", funded from the Civil Initiatives Fund of the Ministry of Labor and Social Policy (FIO 2008, contract No. 813).
Weryfikacja:
Politechnika Gdańska

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