The electrochemical response to glassy carbon electrode modification steps towards viral electrochemical immunosensor preparation

Opis

The dataset contains the electrochemical measurements (CV/EIS) carried out for the consecutive steps of the glassy carbon GC electrode functionalization, in separate subfolders, as described. The cyclic voltammetry (CV) was conducted using a Palmsens 4 potentiostat/galvanostat system (Methrom, Autolab, Netherlands) in the standard three-electrode configuration, with the Pt mesh was used as a counter electrode, and Ag/AgCl/0.1M KCl as a reference electrode. In the case of the EIS, the frequency ranged from 10 kHz to 0.2 Hz for Au electrode with 40 points. The amplitude of the alternating current (AC) signal was 10 mV. Each potential was held constant for 5 s before each measurement to obtain steady-state conditions. All electrochemical tests were carried out in 5 mM K₃[Fe(CN)₆]/K₄[Fe(CN)₆]/in 0.01 M PBS that was previously deaerated. The voltammetric behavior of the modified gold electrode in the presence of a simple redox system, K₄[Fe(CN)₆] in the supporting electrolyte of 0.1 M KCl, was compared with that of the bare electrode.

The last step of sensors validation was the cross-reactivity tests performed with different pathogens and patients' saliva swabs sick with COVID-19 as positive samples. To prepare saliva swabs for the electrochemical measurement 1 mL of it was diluted in the 1 mL of electrolyte utilized in the whole experiment, but with twice time higher concentration of electrochemical species to obtain the same concentration of 5mM [Fe(CN)6]3-/4- after dilution. The 30 μL of that mixture was then spotted on the surface of each electrode and left for the incubation time estimated in previous measurements for each electrode material. In cross-reactivity tests the same sample of saliva swab was used for multiple samples of prepared sensors, ensuring better reproducibility and comparability of results. All electrochemical measurements were conducted in the same manner as previous tests. The validation of immunosensors included the optimization of cross-reactivity tests with pathogens other than the target pathogen. The cross-reactivity tests were performed with different pathogens and patients' saliva swabs sick with COVID-19 as positive samples

These results are part of the manuscript published in Biosensors and Bioelectronics: DOI 10.1016/j.bios.2022.114222

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Informacje szczegółowe

Rok publikacji:

2022

Data zatwierdzenia:

2022-08-01

Język danych badawczych:

angielski

Dyscypliny:

nauki chemiczne (Dziedzina nauk ścisłych i przyrodniczych)

DOI:

10.34808/xkv6-p535

Finansowanie:

iCovid, platforma impedancyjna do ilościowej analizy interakcji wirus-receptor z użyciem diamentowych nanostruktur domieszkowanych borem

Weryfikacja:

Politechnika Gdańska

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