A cryptic ribosome binding site, false signals in reporter systems and avoidance of protein translation chaos - Publikacja - MOST Wiedzy

Twoje konto

zaloguj

Wyszukiwarka

A cryptic ribosome binding site, false signals in reporter systems and avoidance of protein translation chaos

Abstrakt

The expression of reporter gene may be induced by activation of cryptic signalling sequences, as we found while constructing the mutS-lacZ fusion gene. We cloned the Escherichia coli lacZ gene encoding beta-galactosidase into a plasmid vector carrying the Thermus thermophilus mutS gene. The clones expected to produce beta-galactosidase as the C-terminal fusion were selected for the complementation of beta-galactosidase activity in a lacZ deficient E. coli strain. Surprisingly, one of the clones, though displaying beta-galactosidase activity, did not produce the fusion protein. As shown by DNA sequencing a 92 bp fragment in the 3' part of mutS gene was substituted by a 19 bp sequence. As the consequence of the resultant frameshift, a truncated MutS peptide was translated instead of beta-galactosidase fusion. The cloned lacZ gene lacked its ribosome binding site, so lacZ expression could be explained by activation of a cryptic ribosome binding site in the 3' end of mutS gene. This observation shows that fusion domains in reporter systems are possible to produce accidentally misleading signals. This observation also suggests that some triplets like AGG and AGA, present in the canonical ribosome binding sequence, are rarely used codons to prevent chaotic protein translation.

Cytowania

  • 1

    CrossRef

  • 0

    Web of Science

  • 1

    Scopus

Autorzy (5)

Cytuj jako

Pełna treść

pełna treść publikacji nie jest dostępna w portalu

Słowa kluczowe

Informacje szczegółowe

Kategoria:
Publikacja w czasopiśmie
Typ:
artykuł w czasopiśmie wyróżnionym w JCR
Opublikowano w:
JOURNAL OF BIOTECHNOLOGY nr 143, strony 169 - 172,
ISSN: 0168-1656
Język:
angielski
Rok wydania:
2009
Opis bibliograficzny:
Sachadyn P., Stanisławska-Sachadyn A., Kabat E., Zieli Ńska A., Kur J.: A cryptic ribosome binding site, false signals in reporter systems and avoidance of protein translation chaos// JOURNAL OF BIOTECHNOLOGY. -Vol. 143, nr. nr 3 (2009), s.169-172
DOI:
Cyfrowy identyfikator dokumentu elektronicznego (otwiera się w nowej karcie) 10.1016/j.jbiotec.2009.07.001
Weryfikacja:
Politechnika Gdańska

wyświetlono 62 razy

Publikacje, które mogą cię zainteresować

Fusion of DNA-binding domain of Pyrococcus furiosus ligase with TaqStoffel DNA polymerase as a useful tool in PCR with difficult targets

2018

Characterization of a cryptic plasmid pSFKW33 from Shewanella sp. 33b

2009

Novel single-stranded DNA-binding protein from psyvhrophilic bacterium Psychrobacter arcticus

  • M. Nowak,
  • M. (. Olszewski,
  • J. Kur
2011

An examination of an Antarctic soil metagenomic-derivate putative methylthioadenosine phosphorylase gene as a novel reporter gene for promoter trapping

2012
Meta Tagi