The development of new methods for identifying a broad spectrum of analytes, as well as highly selective tools to provide the most accurate information regarding the processes and relationships in the world, has been an area of interest for researchers for many years. The information obtained with these tools provides valuable data to complement existing knowledge but, above all, to identify and determine previously unknown hazards. Recently, attention has been paid to the migration of xenobiotics from the surfaces of various everyday objects and the resulting impacts on human health. Since children are among those most vulnerable to health consequences, one of the main subjects of interest is the migration of low-molecular-weight compounds from toys and products intended for children. This migration has become a stimulus for research aimed at determining the degree of release of compounds from popular commercially available chocolate/toy sets. One of main objectives of this research was to determine the impact of time on the ecotoxicity (with Vibrio fischeri bioluminescent bacteria) of extracts of products intended for children and to assess the correlation with total volatile organic compound emissions using basic chemometric methods. The studies on endocrine potential (with XenoScreen YES/YAS) of the extracts and showed that compounds released from the studied objects (including packaging foils, plastic capsules storing toys, most of toys studied and all chocolate samples) exhibit mostly androgenic antagonistic behavior while using artificial saliva as extraction medium increased the impact observed. The impact of time in most cases was positive one and increased with prolonging extraction time.
Web of Science
- Publikacja w czasopiśmie
- artykuł w czasopiśmie wyróżnionym w JCR
- Opublikowano w:
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
strony 282 - 289,
- Rok wydania:
- Opis bibliograficzny:
- Szczepańska N., Marć M., Kudłak B., Simeonov, V., Tsakovski S., Namieśnik J.: Assessment of ecotoxicity and total volatile organic compound (TVOC) emissions from food and children's toy products// ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY. -Vol. 160, (2018), s.282-289
- Cyfrowy identyfikator dokumentu elektronicznego (otwiera się w nowej karcie) 10.1016/j.ecoenv.2018.05.042
- Bibliografia: test
- research were: sodium chloride (Sigma Aldrich, Germany), dipotassium phosphate (Ciech S.A., Poland), calcium chloride (Eurochem BGD, Poland), magnesium chloride, potassium chloride, potassium carbonate, lactic acid, urea (POCH S.A., Poland), acetic acid (35-38% w/w), ammonium hydroxide (25 % w/w), (Chempur, Poland), distilled water. Microtox ® 500 kit (Microtox Diluent, 2 % NaCl, lyophilized Vibrio fischeri, Osmotic Adjusting Solution (OAS), Microtox Acute Reagent, Reconstitution Solution (RS) was purchased from ModernWater Ltd. (GB). Ethanol (EtOH, CAS no. 64-17-5), acetic acid (CAS no. 64-19-7), dimethyl sulfoxide (DMSO, CAS no. 67-68-5), and Parafilm ® were purchased from Sigma-Aldrich (Germany). All reagents were of analytical grade or higher.
- XenoScreen YES/YAS reagents (CPRG (chlorophenol red-β-D-galactopyranoside), basal medium, copper sulfate, L-aspartic acid, L-threonine, hAR and hER yeast cells stabilized on the filtration paper, vitamin, vials with calibrators: 5α-dihydrotestosterone, 17β-estradiol, flutamide, 4-hydroxytamoxifen) were purchased from Xenometrics G. A. (Switzerland). culture flasks with gas-permeable filter caps, gas-permeable plate sealers, 96-well plates, were purchased from GenoPlast Biochemicals (Poland). The equipment and instruments used were: otwiera się w nowej karcie
- Microtox ® 500 (Modern Water Ltd. (GB)), electronic multi-and single-channel pipettes (Eppendorf (Germany)), CP411 pH-meter (Metron (Poland)), shaker type water bath 357 (Elpan Laboratory Instruments (Poland)). otwiera się w nowej karcie
- Supplementary Material for methodology Click here to download Supplementary Material: Supplementary material for methodology.docx otwiera się w nowej karcie
- Źródła finansowania:
- The work has been co-financed by the National Science Center, Poland, Grant no. 2015/17/N/ST4/03835. The support of H2020 program of the European Union (project Materials Networking no. 692146) is gratefully acknowledged by Prof. V. Simeonov and Prof. S. Tsakovski. Mariusz Marć is grateful for financial support from Ministry of Science and Higher Education (Grant no. 4914/E-359/M/2016).
- Politechnika Gdańska
wyświetlono 44 razy