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Abstrakt
Production of extracellular protease by extremophilic bacteria Deinococcus geothermalis cultivated in liquid media containing 0.1% (w/v) of peptone K, 0.1% yeast extract and 0.2% marine salt reached a maximum in 14 h of the cell growth at 45°C and pH 8.0. The enzyme was purified by a two-step procedure using fractionation by a graded ammonium sulphate precipitation technique and gel filtration on Sephadex G-100 column. Protease from D. geothermalis with a molecular mass of 24 kDa was active at 60°C and pH 9.0. The enzyme solution was stable for 1 h at 60°C and displayed about 60% of the initial activity after 1 h incubation at pH values 5.0 and 11. The phenylmethanesulfonyl fluoride (PMSF) at 1 mM concentration decreased proteolytic activity up to 27.4% of the initial value and it suggests that the enzyme is a serine protease. The activity was stimulated by Ca2+, Na+, Mg2+ and strongly inhibited by Hg2+, Cu2+, Zn2+ and Fe3+. Nonionic detergents like Triton X-100 and Tween 80 did not affect catalytic properties. It suggested that the enzyme produced by D. geothermalis could be used as a component of detergents.
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Informacje szczegółowe
- Kategoria:
- Publikacja w czasopiśmie
- Typ:
- artykuły w czasopismach recenzowanych i innych wydawnictwach ciągłych
- Opublikowano w:
-
AFRICAN JOURNAL OF BIOTECHNOLOGY
nr 12,
strony 4020 - 4027,
ISSN: 1684-5315 - Język:
- angielski
- Rok wydania:
- 2013
- Opis bibliograficzny:
- Pietrow-Tobiszewska O., Panek A., Synowiecki J.: Extracellular proteolytic activity of Deinococcus geothermalis// AFRICAN JOURNAL OF BIOTECHNOLOGY. -Vol. 12., nr. 25 (2013), s.4020-4027
- Weryfikacja:
- Politechnika Gdańska
wyświetlono 78 razy
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