PCR and real-time PCR assays to detect fungi of Alternaria alternata species - Publikacja - MOST Wiedzy


PCR and real-time PCR assays to detect fungi of Alternaria alternata species


Fungi of the Alternaria genus are mostly associated with allergic diseases. However, with a growing number of immunocompromised patients, these fungi, with A. alternata being the most prevalent one, are increasingly recognized as etiological agents of infections (phaeohyphomycoses) in humans. Nowadays, identification of Alternaria spp. requires their pure culture and is solely based on morphological criteria. Clinically, Alternaria infections may be indistinguishable from other fungal diseases. Therefore, a diagnostic result is often delayed or even not achieved at all. In this paper we present easy to perform and interpret PCR and real-time PCR assays enabling detection of A. alternata species. On the basis of alignment of β-tubulin gene sequences, A. alternata-specific primers were designed. DNA from fungal isolates, extracted in a two-step procedure, were used in PCR and real-time PCR assays followed by electrophoresis or melting temperature analysis, respectively. The assays specificity was confirmed, since positive results were obtained for all A. alternata isolates, and no positive results were obtained neither for other molds, dermatophytes, yeast-like fungi, nor human DNA. The assays developed here enable fast and unambiguous identification of A. alternata pathogens.


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Publikacja w czasopiśmie
artykuł w czasopiśmie wyróżnionym w JCR
Opublikowano w:
Acta Biochimica Polonica nr 62, strony 707 - 712,
ISSN: 0001-527X
Rok wydania:
Opis bibliograficzny:
Kordalewska M., Brillowska-Dąbrowska A., Jagielski T., Dworecka-Kaszak B.: PCR and real-time PCR assays to detect fungi of Alternaria alternata species// Acta Biochimica Polonica. -Vol. 62, nr. 4 (2015), s.707-712
Cyfrowy identyfikator dokumentu elektronicznego (otwiera się w nowej karcie) 10.18388/abp.2015_1112
Politechnika Gdańska

wyświetlono 44 razy

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