Probiotic potential of Bacillus Isolates from Polish Bee Pollen and Bee Bread

Karolina Matejczuk; Ahmer Hafeez; Randy Worobo; Piotr Szweda

doi:10.1007/s12602-023-10157-4

Probiotic potential of Bacillus Isolates from Polish Bee Pollen and Bee Bread

Abstrakt

The main goal of this study was the evaluation of the probiotic potential of 10 Bacillus spp. strains isolated from 5 bee bread and 3 bee pollen samples. The antagonistic interaction with Staphylococcus aureus and Escherichia coli was a primary criterion for the preliminary selection of the isolates. Three out of ten strains—PY2.3 (isolated from pollen), BP20.15 and BB10.1 (both isolated from bee bread)—were found to be possible probiotic strains. All these strains are safe for humans (exhibiting -hemolytic activity) and meet all essential requirements for probiotics in terms of viability in the presence of bile salts and acid conditions, hydrophobicity, auto-aggregation, and co-aggregation with the cells of important human pathogenic bacteria. They also assimilate more than 30% of cholesterol after 24 h of incubation. These three isolates are resistant to penicillin but sensitive (or exhibit moderate resistance) to the other nine antibiotics tested herein. On the basis of whole-genome sequencing, BP20.15 and BB10.1 were classified as B. subtilis and PY2.3 as B. velezensis. Moreover, genomic analyses revealed that all these isolates are potential producers of different antimicrobial compounds, including bacteriocins and secondary metabolites. The outcomes of this study have proven that some of the Bacillus strains isolated from bee pollen or bee bread are potential probiotics.

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Autorzy (4)

Karolina Matejczuk (dawniej: Karolina Pełka) dr inż.
Ahmer Hafeez
Randy Worobo
Piotr Szweda dr hab. inż.

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Wersja publikacji: Accepted albo Published Version
DOI:: Cyfrowy identyfikator dokumentu elektronicznego (otwiera się w nowej karcie) 10.1007/s12602-023-10157-4
Licencja: otwiera się w nowej karcie

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Kategoria:

Publikacja w czasopiśmie

Typ:

artykuły w czasopismach dostępnych w wersji elektronicznej [także online]

Opublikowano w:

Probiotics and Antimicrobial Proteins
ISSN: 1867-1306

Język:

angielski

Rok wydania:

2023

Opis bibliograficzny:

Pełka K., Hafeez A., Worobo R., Szweda P., Probiotic potential of Bacillus Isolates from Polish Bee Pollen and Bee Bread, Probiotics and Antimicrobial Proteins, 2023,10.1007/s12602-023-10157-4

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