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• Homoisocitrate dehydrogenase from Candida albicans: properties, inhibition, and targeting by an antifungal pro-drug

  Publikacja

  • I. Gabriel
  • N. D. Vetter
  • D. R. J. Palmer
  • M. J. Milewska
  • M. Wojciechowski
  • S. Milewski

  - FEMS YEAST RESEARCH - Rok 2013

  The LYS12 gene from Candida albicans, coding for homoisocitrate dehydrogenase was cloned and expressed as a His-tagged protein in Escherichia coli. The purified gene product catalyzes the Mg2+- and K+-dependent oxidative decarboxylation of homoisocitrate to alpha-ketoadipate. The recombinant enzyme demonstrates strict specificity for homoisocitrate. SDS-PAGE of CaHIcDH revealed its molecular mass of 42.6±1 kDa, while in size-exclusion...

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