Wyniki wyszukiwania dla: esteraza

• Expression of a GDSL esterase from Pseudomonas sp. S9 in Pichiapastoris

  Publikacja

  • M. Wicka-Grochocka
  • H. Cieśliński
  • E. Krajewska
  • N. Filipowicz
  • M. Wanarska
  • J. Kur

  - New Biotechnology - Rok 2016

  Cold active lipolytic enzymes are promising to replace the conventional enzymes processes of biotechnological industries. One of the most important feature of the cold-active lipases and esterases is that they offer economic benefits through energy saving. In general, they exhibit high activity at low temperatures and low thermostability at moderate temperatures. Lipolytic enzyme EstS9 was isolated from Pseudomonas sp. S9. A multiple...

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• Adaptowana do zimna esteraza Pseudoalteromonas sp. 643A - produkcja w systemach ekspresyjnych Escherichia coli, oczyszczanie i charakterystyka

  Publikacja

  • A. Długołęcka

  - Rok 2008

• A cold-adapted esterase from psychrotrophic Pseudoalteromonas sp. strain 643A

  Publikacja

  • H. Cieśliński
  • A. Białkowska
  • A. Długołęcka
  • M. Daroch
  • K. Tkaczuk
  • H. Kalinowska
  • J. Kur
  • M. Turkiewicz

  - ARCHIVES OF MICROBIOLOGY - Rok 2007

  Dzięki zastosowaniu lipaz możliwa jest biotechnologiczna produkcja m.in. biodegradowalnych biopolimerów, biopaliw (tzw. biodiesel), wielu terapeutyków, herbicydów, pestycydów, kosmetyków, substancji smakowych, serów i in. Szczególnie interesującą grupę stanowią enzymy lipolityczne, pochodzące z organizmów psychrofilnych i psychrotrofowych, ze względu na zdolność do wydajnego katalizowania reakcji w temperaturach rzędu 10-20 ºC....

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• BIOSYNTEZA ESTERAZY Z PSEUDOMONAS SP. S9 W KOMÓRKACH PICHIA PASTORIS, OCZYSZCZANIE I CHARAKTERYSTYKA

  Publikacja

  • M. Wicka-Grochocka
  • A. Terebieniec
  • E. Krajewska
  • N. Filipowicz
  • H. Cieśliński
  • M. Wanarska

  - Postępy Mikrobiologii - Rok 2017

  Enzymy lipolityczne ze względu na swoje unikalne właściwości takie jak stereo-, chemo- i regiospecyficzność znajdują szereg zastosowań w różnych gałęziach przemysłu. Lipazy i esterazy wykorzystywane są w przemyśle chemicznym, farmaceutycznym, spożywczym oraz w syntezie organicznej. W ramach wcześniejszych badań zidentyfikowałam i scharakteryzowałam aktywną w niskiej temperaturze esterazę z Pseudomonas sp. S9. Esteraza Pseudomonas...

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• Structure of EstA esterase from psychrotrophic Pseudoalteromonas sp. 643A covalently inhibited by monoethylphosphonate.

  Publikacja

  • A. Brzuszkiewicz
  • E. Nowak
  • Z. Dauter
  • M. Dauter
  • H. Cieśliński
  • A. Długołęcka
  • J. Kur

  - Acta Crystallographica Section F-Structural Biology and Crystallization Communications - Rok 2009

  The crystal structure of the esterase EstA from the cold-adapted bacteriumPseudoalteromonas sp. 643A was determined in a covalently inhibited form at aresolution of 1.35 A˚. The enzyme has a typical SGNH hydrolase structureconsisting of a single domain containing a five-stranded beta-sheet, with threehelices at the convex side and two helices at the concave side of the sheet, and isornamented with a couple of very short helices...

• Purification and biochemical characteristic of a cold-active recombinant esterase from Pseudoalteromonas sp. 643A under denaturing conditions.

  Publikacja

  • A. Długołęcka
  • H. Cieśliński
  • P. Bruździak
  • K. Gottfried
  • M. Turkiewicz
  • J. Kur

  - Polish Journal of Microbiology - Rok 2009

  In this paper production of a cold-active esterase EstA from the Antarctic bacterium Pseudoalteromonas sp. 643A in E. coli expression system was described. The purification and biochemical characteristic of EstA were performed in the presence of urea and then compared with results obtained for the esterase with no addition of urea and isolated from the native source. In both cases the cold-active enzyme displayed similar properties....

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• Cloning, expression in Komagataella phaffii, and biochemical characterization of recombinant sequence variants of Pseudomonas sp. S9 GDSL-esterase

  Publikacja

  • M. Wicka-Grochocka
  • H. Cieśliński
  • M. Wanarska

  - Acta Biochimica Polonica - Rok 2021

  Two recombinant Komagataella phaffii (formerly Pichia pastoris) yeast strains for production of two sequential variants of EstS9 esterase from psychrotolerant bacterium Pseudomonas sp. S9, i.e. αEstS9N (a two-domain enzyme consisting of a catalytic domain and an autotransporter domain) and αEstS9Δ (a single-domain esterase) were constructed. However, only one of recombinant K. phaffii strains, namely Komagataella phaffii X-33/pPICZαestS9Δ,...

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• Cloning, expression, and biochemical characterization of a coldactive GDSL-esterase of a Pseudomonas sp. S9 isolated from Spitsbergen island soil

  Publikacja

  • M. Wicka-Grochocka
  • M. Wanarska
  • E. Krajewska
  • A. Pawlak-Szukalska
  • J. Kur
  • H. Cieśliński

  - Acta Biochimica Polonica - Rok 2016

  An estS9 gene, encoding an esterase of the psychrotolerant bacterium Pseudomonas sp. S9 was cloned and sequenced. The deduced sequence revealed a protein of 636 amino acid residues with a molecular mass of 69 kDa.Further amino acid sequence analysis revealed that the EstS9 enzyme contained a G-D-S-L motif centered at a catalytic serine, an N-terminal catalytic domain and a C-terminal autotransporter domain. Two recombinant E. coli...

  Pełny tekst do pobrania w portalu

• Esteraza GDSL z psychrotolerancyjnego szczepu Pseudomonas sp. S9 - konstrukcja systemów ekspresyjnych opartych o komórki Escherichia coli i Pichia pastoris, produkcja, oczyszczanie i charakterystyka uzyskanych białek rekombinowanych

  Publikacja

  • M. Wicka-Grochocka

  - Rok 2017

  W rozprawie doktorskiej celu opracowania wydajnej metody produkcji rekombinantowych wariantów sekwencyjnych enzymu esterolitycznego z psychrotolerancyjnego szczepu Pseudomonas sp. S9 zaprojektowano układy ekspresyjne umożliwiające ich produkcję w bakteriach Escherichia coli oraz drożdżach Pichia pastoris. W systemie ekspresyjnym E. coli wyprodukowano dwa warianty białek: EstS9Auto (białko zbudowane z domeny katalitycznej, domeny...

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• Extracellular secretion of Pseudoalteromonas sp. cold-adapted esterase in Escherichia coli in the presence of Pseudoalteromonas sp. components of ABC transport system

  Publikacja

  • A. Długołęcka
  • H. Cieśliński
  • M. Turkiewicz
  • A. Białkowska
  • J. Kur

  - PROTEIN EXPRESSION AND PURIFICATION - Rok 2008

  Enzymy lipolityczne, głównie lipazy i esterazy, stanowią cenną grupę biokatalizatorów wykorzystywanych w przemyśle. Coraz większe zainteresowanie tą klasą enzymów sprawiło, że poszukuje się alternatywnych źródeł ich pozyskiwania. W celu zbadania wpływu białek transportu ABC Pseudoalteromonas sp. 643A na ekspresję genu, sekrecję i aktywność esterazy EstA z tego mikroorganizmu w systemie ekspresyjnym E. coli skonstruowano układ do...

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• Ewa Augustin dr hab.

  Osoby

  Katedra Technologii Leków i Biochemii

• Ocena uszkodzeń studni i wpustów ulicznych w Gdańsku

  Publikacja

  • M. Pszczoła
  • R. Edel
  • E. Górecka
  • I. Papkow

  - Przegląd Komunikacyjny - Rok 2015

  W niniejszym artykule przedstawiono metodykę badań oraz wyniki oceny uszkodzeń studni i wpustów ulicznych na wybranych odcinkach ulic w Gdańsku, charakteryzujących się dużym natężeniem ruchu. Łączna długość analizowanych odcinków ulic wynosiła ponad 10 km. Ocenie poddano ponad 500 urządzeń uzbrojenia inżynieryjnego: studni kanalizacji deszczowej i sanitarnej oraz wpustów ulicznych. Stwierdzono trzy najczęściej występujące rodzaje...

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• Estera Różycka mgr

  Osoby

• Mechanism of selective anticancer activity of isothiocyanates relies on differences in DNA damage repair between cancer and healthy cells

  Publikacja

  • A. Hać
  • J. Brokowska
  • E. Rintz
  • M. Bartkowski
  • G. Węgrzyn
  • A. Herman-Antosiewicz

  - EUROPEAN JOURNAL OF NUTRITION - Rok 2020

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• Extensive Drug Resistance of Strong Biofilm-Producing Acinetobacter baumannii Strains Isolated from Infections and Colonization Hospitalized Patients in Southern Poland

  Publikacja

  • T. Kasperski
  • D. Romaniszyn
  • E. Jachowicz-Matczak
  • M. Pomorska-Wesołowska
  • J. Wójkowska-Mach
  • A. Chmielarczyk

  - Pathogens - Rok 2023

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• Estera Zarębska-Biernat

  Osoby

• Synteza koniugatów parasoli molekularnych ze związkami o udowodnionej aktywności przeciwgrzybiczej

  Publikacja

  • A. S. Skwarecki

  - Rok 2017

  W ramach badań, opisanych w niniejszej rozprawie doktorskiej, zaprojektowano, otrzymano oraz scharakteryzowano połączenia tzw. parasoli molekularnych ze związkami o udowodnionej aktywności przeciwgrzybowej – FMDP i cis-pentacyny – będących inhibitorami enzymów grzybowych. Wspomniane cząsteczki aktywne wykazują dobre właściwości przeciwgrzybowe, jednak ze względu na swoją budowę chemiczną i polarny charakter, wykazują silnie ograniczoną...

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• COVID-19: Is There Evidence for the Use of Herbal Medicines as Adjuvant Symptomatic Therapy?

  Publikacja

  • D. Silveira
  • J. Prieto-Garcia
  • F. Boylan
  • O. Estrada
  • Y. Fonseca-Bazzo
  • C. Jamal
  • P. Magalhães
  • E. Pereira
  • M. Tomczyk
  • M. Heinrich

  - Frontiers in Pharmacology - Rok 2020

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• Virulence, antifungal susceptibility and molecular mechanisms of echinocandin resistance among Candida isolates recovered from clinical specimens

  Publikacja

  • M. Mroczyńska-Szeląg

  - Rok 2020

  Fungi of the genus Candida belong to the natural microflora of healthy individuals. However, they can also be a cause of opportunistic infections especially among patients with an impaired immune system. The first line therapy of Candida infections is based on triazoles. However, in recent years there an increase of azole resistant Candida spp., in particular C. glabrata and C. krusei, has been observed. For this reason, echinocandin...

• PROSTATE GLAND, Prostate gland - Male, 74 - Tissue image [3230630012581011]

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  open access

  • Gdański Uniwersytet Medyczny

  This is the histopathological image of PROSTATE GLAND tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.

• Hyperplasia of prostate - Male, 74 - Tissue image [3230630012571571]

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  open access

  • Gdański Uniwersytet Medyczny

  This is the histopathological image of PROSTATE GLAND tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.

• Hyperplasia of prostate - Male, 74 - Tissue image [3230630012579791]

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  open access

  • Gdański Uniwersytet Medyczny

  This is the histopathological image of PROSTATE GLAND tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.

• Hyperplasia of prostate - Male, 74 - Tissue image [323063001257541]

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  open access

  • Gdański Uniwersytet Medyczny

  This is the histopathological image of PROSTATE GLAND tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.

• Hyperplasia of prostate - Male, 74 - Tissue image [3230630012578071]

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  open access

  • Gdański Uniwersytet Medyczny

  This is the histopathological image of PROSTATE GLAND tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.

• Acinar adenocarcinoma of prostate - Male, 69 - Tissue image [530073002587151]

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  open access

  • Gdański Uniwersytet Medyczny

  This is the histopathological image of PROSTATE GLAND tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 1000 slide scanner (20x magnification) and saved to DICOM format.

• Hyperplasia of prostate - Male, 74 - Tissue image [3230630012576031]

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  open access

  • Gdański Uniwersytet Medyczny

  This is the histopathological image of PROSTATE GLAND tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.

• Hyperplasia of prostate - Male, 74 - Tissue image [3230630012576761]

  Dane Badawcze

  open access

  • Gdański Uniwersytet Medyczny

  This is the histopathological image of PROSTATE GLAND tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.

• Hyperplasia of prostate - Male, 74 - Tissue image [323063001257511]

  Dane Badawcze

  open access

  • Gdański Uniwersytet Medyczny

  This is the histopathological image of PROSTATE GLAND tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.

• Acinar adenocarcinoma of prostate - Male, 69 - Tissue image [5300730025877621]

  Dane Badawcze

  open access

  • Gdański Uniwersytet Medyczny

  This is the histopathological image of PROSTATE GLAND tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 1000 slide scanner (20x magnification) and saved to DICOM format.

• Hyperplasia of prostate - Male, 74 - Tissue image [3230630012575601]

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  open access

  • Gdański Uniwersytet Medyczny

  This is the histopathological image of PROSTATE GLAND tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.

• Hyperplasia of prostate - Male, 74 - Tissue image [3230630012576861]

  Dane Badawcze

  open access

  • Gdański Uniwersytet Medyczny

  This is the histopathological image of PROSTATE GLAND tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.

• Hyperplasia of prostate - Male, 74 - Tissue image [3230630012572111]

  Dane Badawcze

  open access

  • Gdański Uniwersytet Medyczny

  This is the histopathological image of PROSTATE GLAND tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.

• Hyperplasia of prostate - Male, 74 - Tissue image [3230630012577611]

  Dane Badawcze

  open access

  • Gdański Uniwersytet Medyczny

  This is the histopathological image of PROSTATE GLAND tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.

• Hyperplasia of prostate - Male, 63 - Tissue image [3130630016436921]

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  open access

  • Gdański Uniwersytet Medyczny

  This is the histopathological image of PROSTATE GLAND tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.

• Hyperplasia of prostate - Male, 63 - Tissue image [3130630016435901]

  Dane Badawcze

  open access

  • Gdański Uniwersytet Medyczny

  This is the histopathological image of PROSTATE GLAND tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.

• Hyperplasia of prostate - Male, 74 - Tissue image [3230630012578491]

  Dane Badawcze

  open access

  • Gdański Uniwersytet Medyczny

  This is the histopathological image of PROSTATE GLAND tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.

• Hyperplasia of prostate - Male, 74 - Tissue image [3230630012585071]

  Dane Badawcze

  open access

  • Gdański Uniwersytet Medyczny

  This is the histopathological image of PROSTATE GLAND tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.

• Hyperplasia of prostate - Male, 74 - Tissue image [330073006943481]

  Dane Badawcze

  open access

  • Gdański Uniwersytet Medyczny

  This is the histopathological image of PROSTATE GLAND tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.

• Hyperplasia of prostate - Male, 74 - Tissue image [3230630012575511]

  Dane Badawcze

  open access

  • Gdański Uniwersytet Medyczny

  This is the histopathological image of PROSTATE GLAND tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.

• Hyperplasia of prostate - Male, 63 - Tissue image [3130630016438901]

  Dane Badawcze

  open access

  • Gdański Uniwersytet Medyczny

  This is the histopathological image of PROSTATE GLAND tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.

• Hyperplasia of prostate - Male, 63 - Tissue image [3130630016437501]

  Dane Badawcze

  open access

  • Gdański Uniwersytet Medyczny

  This is the histopathological image of PROSTATE GLAND tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.

• Hyperplasia of prostate - Male, 74 - Tissue image [3230630012577891]

  Dane Badawcze

  open access

  • Gdański Uniwersytet Medyczny

  This is the histopathological image of PROSTATE GLAND tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.

• Hyperplasia of prostate - Male, 74 - Tissue image [323063001257891]

  Dane Badawcze

  open access

  • Gdański Uniwersytet Medyczny

  This is the histopathological image of PROSTATE GLAND tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.

• Hyperplasia of prostate - Male, 63 - Tissue image [3130630016433921]

  Dane Badawcze

  open access

  • Gdański Uniwersytet Medyczny

  This is the histopathological image of PROSTATE GLAND tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.

• Hyperplasia of prostate - Male, 74 - Tissue image [3230630012576921]

  Dane Badawcze

  open access

  • Gdański Uniwersytet Medyczny

  This is the histopathological image of PROSTATE GLAND tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.

• Hyperplasia of prostate - Male, 74 - Tissue image [3230630012577221]

  Dane Badawcze

  open access

  • Gdański Uniwersytet Medyczny

  This is the histopathological image of PROSTATE GLAND tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.

• Hyperplasia of prostate - Male, 74 - Tissue image [3230630012574491]

  Dane Badawcze

  open access

  • Gdański Uniwersytet Medyczny

  This is the histopathological image of PROSTATE GLAND tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.

• Hyperplasia of prostate - Male, 74 - Tissue image [3230630012575781]

  Dane Badawcze

  open access

  • Gdański Uniwersytet Medyczny

  This is the histopathological image of PROSTATE GLAND tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.

• Hyperplasia of prostate - Male, 74 - Tissue image [3230630012582431]

  Dane Badawcze

  open access

  • Gdański Uniwersytet Medyczny

  This is the histopathological image of PROSTATE GLAND tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.

• Hyperplasia of prostate - Male, 74 - Tissue image [3230630012578141]

  Dane Badawcze

  open access

  • Gdański Uniwersytet Medyczny

  This is the histopathological image of PROSTATE GLAND tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.