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Open Research Data
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Reactivity of recombinant B1 protein with specific anti-B. burgdorferi s.l. antibodies present in human sera
Open Research DataBiotechnologically produced B1 antigens from three genospecies of Borrelia burgdorferi s.l. were assessed for their reactivity with specific anti-B. burgdorferi s.l. antibodies by IgG/IgM Western blot and IgG/IgM ELISA to determine their utility for serological diagnosis of Borrelia infection in human sera.
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Reactivity of recombinant B12 protein with specific anti-B. burgdorferi s.l. antibodies present in human sera
Open Research DataBiotechnologically produced B12 antigens from three genospecies of Borrelia burgdorferi s.l. were assessed for their reactivity with specific anti-B. burgdorferi s.l. antibodies by Western blot to determine their utility for serological diagnosis of Borrelia infection in human sera.
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Reactivity of recombinant B2 protein with specific anti-B. burgdorferi s.l. antibodies present in human sera
Open Research DataBiotechnologically produced B2 antigens from three genospecies of Borrelia burgdorferi s.l. were assessed for their reactivity with specific anti-B. burgdorferi s.l. antibodies by Western blot to determine their utility for serological diagnosis of Borrelia infection in human sera.
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Reactivity of recombinant B6 protein with specific anti-B. burgdorferi s.l. antibodies present in human sera
Open Research DataBiotechnologically produced B6 antigens from three genospecies of Borrelia burgdorferi s.l. were assessed for their reactivity with specific anti-B. burgdorferi s.l. antibodies by Western blot to determine their utility for serological diagnosis of Borrelia infection in human sera.
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Reactivity of recombinant B3 protein with specific anti-B. burgdorferi s.l. antibodies present in human sera
Open Research DataBiotechnologically produced B3 antigens from three genospecies of Borrelia burgdorferi s.l. were assessed for their reactivity with specific anti-B. burgdorferi s.l. antibodies by IgG/IgM Western blot and IgG/IgM ELISA to determine their utility for serological diagnosis of Borrelia infection in human sera.
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Construction of recombinant plasmid coding Borrelia burgdorferi sl P protein
Open Research DataDiagnosis of Lyme borreliosis (LB), an infection caused by spirochaetes of the B. burgdorferi sl species complex, is mainly based on clinical symptoms supported with serology and is often misdiagnosed in areas of endemicity. The complexity of the antigenic composition among the Borrelia genospecies and differential expression of proteins in host and...
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Biotechnological production and purification of Borrelia burgdorferi sl P protein
Open Research DataBorrelia burgdorferi P protein could be used in the detection of specific immunoglobulins G (IgG) from patients' sera with Lyme disease that may be infected with different genospecies of B. burgdorferii sl. The goal of the study was to obtain the highest possible yield of protein expression in prokaryotic cells and purified protein preparations. This...
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Construction of recombinant plasmid coding Borrelia burgdorferii sl F protein
Open Research DataDiagnosis of Lyme borreliosis (LB), an infection caused by spirochaetes of the B. burgdorferi sl species complex, is mainly based on clinical symptoms supported with serology and is often misdiagnosed in areas of endemicity. The complexity of the antigenic composition among the Borrelia genospecies and differential expression of proteins in host and...
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Biotechnological production and purification of Borrelia burgdorferi sl F protein
Open Research DataBorrelia burgdorferi F protein could be used in the detection of specific immunoglobulins G (IgG) from patients' sera with Lyme disease that may be infected with different genospecies of B. burgdorferii sl. The goal of the study was to obtain the highest possible yield of protein expression in prokaryotic cells and purified protein preparations. This...
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Construction of recombinant plasmid coding Borrelia burgdorferi sl B1 protein
Open Research DataDiagnosis of Lyme borreliosis (LB), an infection caused by spirochaetes of the B. burgdorferi sl species complex, is mainly based on clinical symptoms supported with serology and is often misdiagnosed in areas of endemicity. The complexity of the antigenic composition among the Borrelia genospecies and differential expression of proteins in host and...
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Biotechnological production and purification of Borrelia burgdorferi sl B12 protein
Open Research DataBorrelia burgdorferi B12 protein could be used in the detection of specific immunoglobulins from patients' sera with Lyme disease that may be infected with different genospecies of B. burgdorferii sl. The goal of the study was to obtain the highest possible yield of protein expression in prokaryotic cells and purified protein preparations. This dataset...
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Biotechnological production and purification of Borrelia burgdorferi sl B1 protein
Open Research DataBorrelia burgdorferi B1 protein could be used in the detection of specific immunoglobulins from patients' sera with Lyme disease that may be infected with different genospecies of B. burgdorferii sl. The goal of the study was to obtain the highest possible yield of protein expression in prokaryotic cells and purified protein preparations. This dataset...
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Construction of recombinant plasmid coding Borrelia burgdorferi sl B2 protein
Open Research DataDiagnosis of Lyme borreliosis (LB), an infection caused by spirochaetes of the B. burgdorferi sl species complex, is mainly based on clinical symptoms supported with serology and is often misdiagnosed in areas of endemicity. The complexity of the antigenic composition among the Borrelia genospecies and differential expression of proteins in host and...
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Biotechnological production and purification of Borrelia burgdorferi sl B2 protein
Open Research DataBorrelia burgdorferi B2 protein could be used in the detection of specific immunoglobulins from patients' sera with Lyme disease that may be infected with different genospecies of B. burgdorferii sl. The goal of the study was to obtain the highest possible yield of protein expression in prokaryotic cells and purified protein preparations. This dataset...
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Construction of recombinant plasmid coding Borrelia burgdorferi sl B3 protein
Open Research DataDiagnosis of Lyme borreliosis (LB), an infection caused by spirochaetes of the B. burgdorferi sl species complex, is mainly based on clinical symptoms supported with serology and is often misdiagnosed in areas of endemicity. The complexity of the antigenic composition among the Borrelia genospecies and differential expression of proteins in host and...
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Biotechnological production and purification of Borrelia burgdorferi sl B3 protein
Open Research DataBorrelia burgdorferi B3 protein could be used in the detection of specific immunoglobulins from patients' sera with Lyme disease that may be infected with different genospecies of B. burgdorferii sl. The goal of the study was to obtain the highest possible yield of protein expression in prokaryotic cells and purified protein preparations. This dataset...
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Construction of recombinant plasmid coding Borrelia burgdorferi sl B6 protein
Open Research DataDiagnosis of Lyme borreliosis (LB), an infection caused by spirochaetes of the B. burgdorferi sl species complex, is mainly based on clinical symptoms supported with serology and is often misdiagnosed in areas of endemicity. The complexity of the antigenic composition among the Borrelia genospecies and differential expression of proteins in host and...
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Construction of recombinant plasmid coding Borrelia burgdorferi sl B12 protein
Open Research DataDiagnosis of Lyme borreliosis (LB), an infection caused by spirochaetes of the B. burgdorferi sl species complex, is mainly based on clinical symptoms supported with serology and is often misdiagnosed in areas of endemicity. The complexity of the antigenic composition among the Borrelia genospecies and differential expression of proteins in host and...
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Biotechnological production and purification of Borrelia burgdorferi sl B6 protein
Open Research DataBorrelia burgdorferi B6 protein could be used in the detection of specific immunoglobulins from patients' sera with Lyme disease that may be infected with different genospecies of B. burgdorferii sl. The goal of the study was to obtain the highest possible yield of protein expression in prokaryotic cells and purified protein preparations. This dataset...
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Impact of binary mixtures of buthylparaben and 17α-ethinylestradiol on endocrine processes of the model organism Saccharomyces Cerevisiae (using XenoScreen YES/YAS test) - with controls
Open Research DataData shown in data set illustrate the results of the endocrine processes of mixtures of buthylparaben and 17α-ethinylestradiol with controls for YES agonsit, YES antagonist, YAS agonist and YAS antagonist. S cerevisiae is used as a model organism in the monitoring of endocrine disruptors in environmental and reference samples in Xenometrix® systems...
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Impact of binary mixtures of methylparaben and 17α-ethinylestradiol on endocrine processes of the model organism Saccharomyces Cerevisiae (using XenoScreen YES/YAS test) - with controls
Open Research DataData shown in data set illustrate the results of the endocrine processes of mixtures of methylparaben and β-estradiol with controls for YES agonsit, YES antagonist, YAS agonist and YAS antagonist. S cerevisiae is used as a model organism in the monitoring of endocrine disruptors in environmental and reference samples in Xenometrix® systems - XenoScreen®. ...
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Data used for article "Chemical composition and techno-functional properties of high-purity water-soluble keratein and its enzymatic hydrolysates"
Open Research DataThe dataset contains data regarding chicken feather keratin isolate (KI) obtained by reductive extraction with L-cysteine and its enzymatic hydrolysates produced by trypsin (KI-T), chymotrypsin (KI-C), pepsin (KI-P) and subtilisin (KI-S). The dataset includes data on the keratin extraction yield (% of feather solubilization), data from high-performance...
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The electrochemical studies for chitosan hydrogel membranes on glassy carbon substrates
Open Research DataThis dataset contains various electrochemical studies carried out to investigate the preconcentration of [Fe(CN)6]3- ions by chitosan hydrogel membranes coating the glassy carbon electrodes. The glassy carbon electrode (GCE, 3.0 mm diameter) was first polished with Al2O3 powder (0.3 mm) using a polishing kit from Buehler, rinsed with deionized...
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DNA G-quadruplex structures obtained through MD-based folding procedure
Open Research DataThe dataset contains structures of DNA G-quadruplexes (G4s), obtained through steered molecular dynamics simulations, for 128 oligonucleotides capable of forming 3- and 2-tetrad G4s. The general sequence of the oligonucleotides is GxTiGxTjGxTkGx, where x = 3 or 2 for 3- and 2-tetrad G4s, respectively, while i, j, and k vary independently from 1 to 4,...
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Impact of binary mixtures of triclosan and β-estradiol on endocrine processes of the model organism Saccharomyces Cerevisiae (using XenoScreen YES/YAS test) - with controls
Open Research DataData shown in data set illustrate the results of the endocrine processes of mixtures of triclosan and β-estradiol with controls for YES agonsit, YES antagonist, YAS agonist and YAS antagonist. S cerevisiae is used as a model organism in the monitoring of endocrine disruptors in environmental and reference samples in Xenometrix® systems - XenoScreen®. ...
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Impact of binary mixtures of triclosan and 17α-ethinylestradiol on endocrine processes of the model organism Saccharomyces Cerevisiae (using XenoScreen YES/YAS test) - with controls
Open Research DataData shown in data set illustrate the results of the endocrine processes of mixtures of triclosan and 17α-ethinylestradiol with controls for YES agonsit, YES antagonist, YAS agonist and YAS antagonist. S cerevisiae is used as a model organism in the monitoring of endocrine disruptors in environmental and reference samples in Xenometrix® systems -...
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XRD database for SnS-based electrode material
Open Research DataXRD results of tin sulfide based material with chitosan as a source of carbon for battery application obtained by a) solvothermal method and b) solvothermal method followed by pyrolysis.
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The analysis of the expression of TEL-F and IFNB1 genes in anthraquinone-treated NSCLC cells
Open Research DataThe data include Ct values of TEL-F and IFNB1 genes from samples subjected to long-term treatment with C4.
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Impact of binary mixtures of triclosan and methylparaben on endocrine processes of the model organism Saccharomyces Cerevisiae (using XenoScreen YES/YAS test) - with controls
Open Research DataData shown in data set illustrate the results of the endocrine processes of mixtures of triclosan and methylparaben with controls for YES agonsit, YES antagonist, YAS agonist and YAS antagonist. S cerevisiae is used as a model organism in the monitoring of endocrine disruptors in environmental and reference samples in Xenometrix® systems - XenoScreen®....
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Impact of binary mixtures of two parabens (Methylparaben and Buthylparaben) on endocrine processes of the model organism Saccharomyces Cerevisiae (using XenoScreen YES/YAS test)
Open Research DataData shown in data set illustrate the results of the endocrine processes of mixtures of two parabens e.i. methylparaben and buthylparaben. S cerevisiae is used as a model organism in the monitoring of endocrine disruptors in environmental and reference samples in Xenometrix® systems - XenoScreen®. Tested substances have bacteriostatic and fungicide...
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The influence of chitosan hydrogel cross-linking by agarose on coating physico-chemical properties
Open Research DataThis dataset contains various physicochemical analyses showing the effect of different concentration of chitosan and the cross-linking agent agarose. Each sample is labeled by C and A representing chitosan and agarose concentrations, respectively, while the exact amounts are depicted in the attached table. Fourier-transform infrared (FT-IR) spectroscopy...
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The influence of chitosan hydrogel coating on different redox probes kinetics
Open Research DataThis dataset contains cyclic voltammetry studies of three redox probes: 1 mM K3[Fe(CN)6], [K4Fe(CN)6], C12H14FeO2, and [Ru(NH3)6]Cl2 dissolved in 0.1 M KCl or PBS as the primary electrolyte. The studies are made for glassy carbon electrode (GC) and glassy carbon electrode covered with thin chitosan hydrogel membrane (C2A2). Cyclic voltammograms were...
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Raman studies of 3D printed CB-PLA samples after microwave treatment
Open Research DataThis dataset contains Raman spectroscopy analyses of microwave-activated carbon-black doped polylactic acid 3D printed electrodes.
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SEM micrographs of 3D printed CB-PLA samples topography after microwave treatment
Open Research DataThis dataset contains the electrochemical studies on microwave-activated carbon-black doped polylactic acid 3D printed electrodes.
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SEM micrographs of 3D printed CB-PLA samples topography after microwave treatment
Open Research DataThis dataset contains the SEM micrographs of carbon-black doped polylactic acid 3D printed electrodes, after microwave treatment to activate the electrode surfaces. FEI Quanta 250 FEG SEM was used for these analyses. Samples were treated with CEM reactor or microwave oven.
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Impact of binary mixtures of diclofenac and buthylparaben of the model organism Saccharomyces Cerevisiae (using XenoScreen YES/YAS test)
Open Research DataData shown in data set illustrate the results of the edorin processes of miture of diclofenac and buthylparaben on S cerevisiae is used as a model organism in the monitoring of endocrine disruptors in environmental and reference samples in Xenometrix® systems - XenoScreen®. Tested substances have bakeriosis and fungicide properties and are used in...
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Impact of binary mixtures of diclofenac and on endocrine processes of the model organism Saccharomyces Cerevisiae (using XenoScreen YES/YAS test)
Open Research DataData shown in data set highlighte the results of the edorin processes of the diclofenac sodium salt mixture with Methylparaben. S cerevisiae is used as a model organism in the monitoring of endocrine disruptors in environmental and reference samples in Xenometrix® systems - XenoScreen®. Methylparaben have fungicide properties used in food preservative,...
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Impact of binary mixtures of Irgasan (triclosan) and Diclofenac on endocrine processes of the model organism Saccharomyces Cerevisiae (using XenoScreen YES/YAS test)
Open Research DataThe XenoScreen YES/YAS reagents and methodology is given in Kudłak et al. (2019) and in details in electronic supplementary material.
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Impact of binary mixtures of Irgasan (triclosan) and two parabens on endocrine processes of the model organism Saccharomyces Cerevisiae (using XenoScreen YES/YAS test)
Open Research DataData shown in data set illustrate the results of the edorin processes of the Irgasan (triclosan) mixture with two parabens e.i. methylparaben and buthylparaben. S cerevisiae is used as a model organism in the monitoring of endocrine disruptors in environmental and reference samples in Xenometrix® systems - XenoScreen®. Tested substances have bakeriosis...
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Results of pharmaceuticals mixtures toxicity on inhibition of bioluminescence of Vibrio fischeri (Microtox)
Open Research DataThe research was concerned with verifying the impact of mixtures of nine pharmaceuticals against a selected organism, i.e., the bacterium Aliivibrio fischeri. A. fisheri is used as a model organism in the monitoring of acute toxicity in environmental and reference samples in Microtox® systems. Tested pharmaceuticals, namely: diclofenac (sodium salt),...
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Determination of the effective concentration of oxyteracycline solutions on the inhibition of bioluminescence of Vibrio fischeri (Microtox)
Open Research DataIn this study an attempt was made to determine the effective concentration for oxytetracycline solutions on the inhibition of bioluminescence of Vibrio fischeri (Microtox).
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XRD for MoS2-Carbon Based Materials
Open Research DataThe presented data showcases the results of XRD analysis conducted on molybdenum sulfide modified with carbon. The MoS2-carbon base materials were prepared via a facile hydrothermal method. Structural characterization confirmed the successful incorporation of carbon into the MoS2 support. XRD was recorded using an X-ray diffractometer (Philips X”Pert...
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Plasmids pMET2M1 and pMET15M1 with the SAT1 Flipper knockout cassette construction
Open Research DataThe goal of the study was to obtain plasmids containing the SAT1 Flipper knockout cassette along with upstream and downstream gene fragments (MET2, MET15) and to introduce a cleavage site for restriction enzymes into them.
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Minimum inhibitory concentrations (MICs) determination of selected human topoisomerase II alpha and bacterial DNA gyrase inhibitors against fungal strains
Open Research DataThe datasets contain the results of determining the MIC (Minimal Inhibitory Concentration) value of known compounds* (inhibitors of human topoisomerase II alpha and bacterial DNA gyrase) against C. albicans SC5314, C. glabrata ATCC 90030, C. krusei ATCC 6258 and C. parapsilosis ATCC 22019 and Saccharomyces cerevisiae ATCC 9763 by the modified M27-A3...
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The effect of pH change of oxyteracycline solutions on the on inhibition of bioluminescence of Vibrio fischeri (Microtox)
Open Research DataIn this study attempt was also made to determine whether a pH change of the sample containing oxyteracycline solutions affects the total toxicity of the sample using the Microtox®
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Impact of binary mixtures of ketoprofen and oxytetracycline on the germination of Sorghum bicolor (sorgo) seeds
Open Research DataResearch was carried out for various ratios of the previously determined EC50 values for mixture of ketoprofen and oxytetracycline the first substance at 100% of its EC50 concentration was mixed with the second substance at 100% of its EC50 concentration, or the first substance at 50% of its EC50 concentration was mixed with the second substance at...
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Assay of Cystathionine-γ-synthase (CaStr2p) activity determination.
Open Research DataThe study aimed to obtain conditions optimal for the activity of Candida albicans cystathionine-γ-synthase (CaStr2p) determination. The selection of appropriate reaction conditions included the identification of the reaction buffer and its pH, substrate, and enzyme concentrations. The activity of Str2p was measured by the detection of decrease of the...
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Effect of antitumor compounds on the yeast topoisomerase II relaxation activity
Open Research DataThe datasets contain results of antitumor compounds* (known inhibitors of human topoisomerase alpha II) inhibitory activity against yeast topoisomerase II. DNA topoisomerases (Topo) are enzymes that catalyze changes in the spatial structure of DNA and play an important role in replication, transcription and recombination. Beyond their normal functions,...
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Overproduction of CaStr2p native and His-tag versions.
Open Research DataEnzyme of fungal L-methionine biosynthetic pathway: cystathionine-γ-synthase (CaStr2p) could be exploited as molecular target for antifungal chemotherapy. The goal of the study was to obtain conditions optimal for protein production with the use of prokaryotic cells. The constructed expression plasmids, designed in three different versions: encoding...
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Investigation of the C-1311 glucuronidation: an electrochemical approach
Open Research DataThis study was undertaken to investigate the glucuronidation of the compound C-1311 (5-diethylaminoethylamino-8-hydroxyimidazoacridinone – the model anticancer acridine derivative) using electrochemistry/mass spectrometry (EC/MS) as a complementary technique to in vitro (liver microsomes) and in silico approaches.