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Detection of the Plant Pathogen Pseudomonas Syringae pv. Lachrymans on Antibody-Modified Gold Electrodes by Electrochemical Impedance Spectroscopy
Abstract
The present work describes an impedimetric immunosensor for Pseudomonas syringae pv. lachrymans (Psl) detection. This pathogen infects many crop species causing considerable yield losses, thus fast and cheap detection method is in high demand. In the assay, the gold disc electrode was modified with 4-aminothiophenol (4-ATP), glutaraldehyde (GA), and anti-Psl antibodies, and free-sites were blocked with bovine serum albumin (BSA). Sensor development was characterized by cyclic voltammetry (CV) and antigen detection by electrochemical impedance spectroscopy (EIS) measurements. Seven analyzed strains of Psl were verified as positive by the reference method (PCR) and this immunoassay, proving sensor specificity. Label-free electrochemical detection was in the linear range 1 × 103–1.2 × 105 CFU/mL (colony-forming unit) with an R2 coefficient of 0.992 and a detection limit (LOD) of 337 CFU/mL. The sensor did not interfere with negative probes like buffers and other bacteria. The assay was proven to be fast (10 min detection) and easy in preparation. The advantage was the simplicity and availability of the verified analyte (whole bacteria) as the method does not require sample pretreatment (e.g., DNA isolation). EIS biosensing technique was chosen as one of the simplest and most sensitive with the least destructive influence on the probes compared to other electrochemical methods.
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Authors (11)
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Zofia Cebula
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Sabina Żołędowska
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Karolina Dziąbowska
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Marta Skwarecka
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Natalia Malinowska
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Wioleta Białobrzeska mgr inż.
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Elżbieta Czaczyk
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Mirosław Sawczak dr hab. inż.
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Dawid Nidzworski Dr
Cite as
Full text
- Publication version
- Accepted or Published Version
- DOI:
- Digital Object Identifier (open in new tab) 10.3390/s19245411
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Keywords
Details
- Category:
- Articles
- Type:
- artykuły w czasopismach
- Published in:
-
SENSORS
no. 19,
ISSN: 1424-8220 - Language:
- English
- Publication year:
- 2019
- Bibliographic description:
- Cebula Z., Żołędowska S., Dziąbowska K., Skwarecka M., Malinowska N., Białobrzeska W., Czaczyk E., Siuzdak K., Sawczak M., Bogdanowicz R., Nidzworski D.: Detection of the Plant Pathogen Pseudomonas Syringae pv. Lachrymans on Antibody-Modified Gold Electrodes by Electrochemical Impedance Spectroscopy// SENSORS -Vol. 19,iss. 24 (2019), s.5411-
- DOI:
- Digital Object Identifier (open in new tab) 10.3390/s19245411
- Verified by:
- Gdańsk University of Technology
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