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The Influence of Storage on Human Milk Lipidome Stability for Lipidomic Studies

Abstract

Human milk (HM) lipidome stability during storage is crucial in lipidomic studies to avoid misinterpretations. Facing the lack of comprehensive work on the HM lipidome stability, we performed a study on a potential alteration in the lipid profiles of HM samples stored under different conditions. An untargeted LC-Q-TOF-MS-based approach was applied to study the influence of storage conditions as well as the interaction of the storage temperature and time on HM lipid profiles. The samples were stored for 4–84 days at temperatures in the range from 4 to −80 °C and also were exposed to up to three freeze–thaw cycles. The results showed that the storage at 4 °C for just 4 days as well as being subjected to three freeze–thaw cycles can lead to a change in the content of lipids. The observed differences in levels of some lipid species in samples stored at −20 °C in comparison to the concentration level of those lipids in samples stored at −80 °C were not statistically significant, and inter-individual variance regardless of sample storage condition was maintained. The storage of HM samples at −20 °C for up to 3 weeks and −80 °C for up to 12 weeks ensures sample lipidome stability.

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DOI:
Digital Object Identifier (open in new tab) 10.1021/acs.jproteome.1c00760
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Details

Category:
Articles
Type:
artykuły w czasopismach
Published in:
JOURNAL OF PROTEOME RESEARCH no. 21, pages 438 - 446,
ISSN: 1535-3893
Language:
English
Publication year:
2022
Bibliographic description:
Garwolińska D., Młynarczyk M., Kot-Wasik A., Hewelt-Belka W.: The Influence of Storage on Human Milk Lipidome Stability for Lipidomic Studies// JOURNAL OF PROTEOME RESEARCH -Vol. 21,iss. 2 (2022), s.438-446
DOI:
Digital Object Identifier (open in new tab) 10.1021/acs.jproteome.1c00760
Sources of funding:
  • COST_FREE
Verified by:
Gdańsk University of Technology

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