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The Influence of Storage on Human Milk Lipidome Stability for Lipidomic Studies

Abstrakt

Human milk (HM) lipidome stability during storage is crucial in lipidomic studies to avoid misinterpretations. Facing the lack of comprehensive work on the HM lipidome stability, we performed a study on a potential alteration in the lipid profiles of HM samples stored under different conditions. An untargeted LC-Q-TOF-MS-based approach was applied to study the influence of storage conditions as well as the interaction of the storage temperature and time on HM lipid profiles. The samples were stored for 4–84 days at temperatures in the range from 4 to −80 °C and also were exposed to up to three freeze–thaw cycles. The results showed that the storage at 4 °C for just 4 days as well as being subjected to three freeze–thaw cycles can lead to a change in the content of lipids. The observed differences in levels of some lipid species in samples stored at −20 °C in comparison to the concentration level of those lipids in samples stored at −80 °C were not statistically significant, and inter-individual variance regardless of sample storage condition was maintained. The storage of HM samples at −20 °C for up to 3 weeks and −80 °C for up to 12 weeks ensures sample lipidome stability.

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Wersja publikacji
Accepted albo Published Version
DOI:
Cyfrowy identyfikator dokumentu elektronicznego (otwiera się w nowej karcie) 10.1021/acs.jproteome.1c00760
Licencja
Creative Commons: CC-BY otwiera się w nowej karcie

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Informacje szczegółowe

Kategoria:
Publikacja w czasopiśmie
Typ:
artykuły w czasopismach
Opublikowano w:
JOURNAL OF PROTEOME RESEARCH nr 21, strony 438 - 446,
ISSN: 1535-3893
Język:
angielski
Rok wydania:
2022
Opis bibliograficzny:
Garwolińska D., Młynarczyk M., Kot-Wasik A., Hewelt-Belka W.: The Influence of Storage on Human Milk Lipidome Stability for Lipidomic Studies// JOURNAL OF PROTEOME RESEARCH -Vol. 21,iss. 2 (2022), s.438-446
DOI:
Cyfrowy identyfikator dokumentu elektronicznego (otwiera się w nowej karcie) 10.1021/acs.jproteome.1c00760
Źródła finansowania:
  • COST_FREE
Weryfikacja:
Politechnika Gdańska

wyświetlono 118 razy

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