Search results for: AMPLIFICATION

• The New LM-PCR/Shifter Method for the Genotyping of Microorganism

  Publication

  • B. Krawczyk
  • K. Stojowska
  • J. Kur

  - Acta Biochimica Polonica - Year 2011

  Techniques relies on the ligation of appropriates adapters (LM-PCR) as AFLP, PCR MP and ADSRRS are successfully used for epidemiological studies for prokaryotic and eukaryotic microorganisms. In this study we propose a new method, called the LM-PCR/Shifter, based on the use of a Class IIS restriction enzyme giving restriction fragments with different 4 base 5' overhangs (Shifter) and the ligation of appropriate oligonucleotide...

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• ALIS-FLP: amplified ligation selected fragment-length polymorphism method for microbial genotyping

  Publication

  • A. Brillowska-Dąbrowska
  • M. Wianecka
  • S. Dąbrowski
  • Z. Mladenovska
  • J. Kur
  • B. Ahring

  - SCANDINAVIAN JOURNAL OF CLINICAL & LABORATORY INVESTIGATION - Year 2008

  A DNA fingerprinting method known as ALIS-FLP (amplified ligation selected fragment-length polymorphism) has been developed for selective and specific amplification of restriction fragments from TspRI restriction endonuclease digested genomic DNA. The method is similar to AFLP, but differs in that only one specific restriction enzyme (TspRI) is used. The cohesive ends of the DNA fragments are ligated with two types of oligonucleotide....

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• Recombinant !ermostable AP Exonuclease from Thermoanaerobacter tengcongensis: Cloning, Expression, Purification, Properties and PCR Application

  Publication

  • S. Dąbrowski
  • A. Brillowska-Dąbrowska
  • B. Ahring

  - Polish Journal of Microbiology - Year 2013

  Apurinic/apyrimidinic (AP) sites in DNA are considered to be highly mutagenic and must be corrected to preserve genetic integrity, especially at high temperatures. !e gene encoding a homologue of AP exonuclease was cloned from the thermophilic anaerobic bacterium Thermoanaerobacter tengcongensis and transformed into Escherichia coli. The protein product showed high identity (80%) to human Ape1 nuclease, whereas to E. coli exonuclease...

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• Analysis of pounding between adjacent buildings founded on different soil types

  Publication

  • M. Miari
  • R. Jankowski

  - SOIL DYNAMICS AND EARTHQUAKE ENGINEERING - Year 2022

  Earthquake-induced pounding was experienced in many previous earthquakes and it was found to be a critical issue. This study investigates the effect of pounding between buildings founded on the same and different soil types. Three 3-D buildings with 4, 6 and 8 storeys were considered in this study. Three pounding scenarios were taken into account, i.e. pounding between 4-storey and 6-storey buildings, between 4-storey and 8-storey...

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• Genotyping of clinical isolates of fluconazole-resistant Candida albicans

  Publication

  • P. Szweda
  • K. M. Gucwa
  • E. Romanowska
  • K. Dzierżanowska-Fangrat
  • S. Milewski

  - Year 2013

  The aim of the study was to compare the discriminatory power of RAPD-PCR method with using RSD10 primer and microsatellite analysis with using (GACA)4 primer for genotyping clinical isolates of fluconazole-resistant C. albicans. Isolates were received from patients of Children's Memorial Health Institute in Warsaw. However in the case of both tested methods low number of amplified fragments was generated, nine and six different...

• Optymalizacja i walidacja metody ADSRRS-fingerprinting - konstrukcja zestawu diagnostycznego do różnicowania genetycznego drobnoustrojów

  Publication

  • B. Krawczyk
  • K. Lewandowski

  - BioTechnologia - Year 2007

  Spośród wszystkich metod typowania mikroorganizmów na szczególną uwagę zasługują metody oparte na analizie całego materiału genetycznego zawartego w komórce. Jedną z nich jest stosunkowo nowa technika ADSRRS-fingerprinting (ang. Amplification of DNA Fragments Surrounding Rare Restriction Sites). Na podstawie naszych badań można stwierdzić, iż charakteryzuje się ona wysokim potencjałem różnicującym, wiarygodnością, powtarzalnością...

• ADSRRS-fingerprinting and PCR MP techniques for studies of intraspecies genetic relatedness in Staphylococcus aureus

  Publication

  • B. Krawczyk
  • J. Leibner
  • W. Barańska-Rybak
  • A. Samet
  • J. Kur

  - JOURNAL OF MICROBIOLOGICAL METHODS - Year 2007

  W pracy pokazano użyteczność dwóch nowych metod do typowania molekularnego Staphylococcus aureus, ADSRRS-fingerprinting (Amplification of DNA fragments Surrounding Rare Restriction Sites) i PCR MP (PCR melting profile). Przebadano 37 szczepów Staphylococcus aureus izolowanych od pacjentów z czyracznością. Jako metodę referencyjną zastosowano REA-PFGE. Okazało się, że izolaty Staphylococcus aureus z nosa od niektórych pacjentów...

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• D-loop sequences retrieved from Canis lupus familiaris mitochondrial genome

  Open Research Data

  open access

  • K. Kowal
  • B. Ślaska
  • A. Tkaczyk-Wlizło

  Canine mitochondrial genome is built of 16727 bp. Non-coding control region (mtCR), called also D-loop, begins with 15458 nucleotide and ends with 16727 nucleotide. The length of this fragment is 1270 bp (Kim et al., 1998). D-loop region is responsible for replication and transcription of mitochondrial DNA. Mutations that occur within it may cause irregularity...

• Experimental study on models of cylindrical steel tanks under mining tremors and moderate earthquakes

  Publication

  • D. Burkacki
  • R. Jankowski

  - Earthquakes and Structures - Year 2019

  The aim of the study is to show the results of complex shaking table experimental investigation focused on the response of two models of cylindrical steel tanks under mining tremors and moderate earthquakes, including the aspects of diagnosis of structural damage. Firstly, the impact and the sweep-sine tests have been carried out, so as to determine the dynamic properties of models filled with different levels of liquid. Then,...

• The use of a one-step PCR method for the identification of Microsporum canis and Trichophyton mentagrophytes infection of pets

  Publication

  • I. Dąbrowska
  • B. Dworecka-Kaszak
  • A. Brillowska-Dąbrowska

  - Acta Biochimica Polonica - Year 2014

  Introduction: Dermatophytes are a closely related group of keratinophilic fungi. They encompass important etiological agents of superficial fungal infections. These fungi are able to invade keratinized tissues of humans and animals, causing dermatophytosis (ringworm) of hair, nails or skin. The aim: Traditional diagnostics of ringworm is based on morphological identification of cultured fungi and is time-consuming. Materials and methods:...

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• A new assay based on terminal restriction fragment length polymorphism of homocitrate synthase gene fragments for Candida species identification

  Publication

  • K. Szemiako
  • A. Śledzińska
  • B. Krawczyk

  - JOURNAL OF APPLIED GENETICS - Year 2017

  Candida sp. have been responsible for an increasing number of infections, especially in patients with immunodeficiency. Species specific differentiation of the Candida sp. is difficult in routine diagnosis. This identification can have a highly significant association in therapy and prophylaxis. This work has shown a new application of terminal restriction fragment length polymorphism (t-RFLP) method in molecular identification...

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• Elemental and tight monogamy relations in nonsignaling theories

  Publication

  • R. Augusiak
  • M. Demianowicz
  • M. Pawłowski
  • J. Tura
  • A. Acín

  - PHYSICAL REVIEW A - Year 2014

  Physical principles constrain the way nonlocal correlations can be distributed among distant parties. These constraints are usually expressed by monogamy relations that bound the amount of Bell inequality violation observed among a set of parties by the violation observed by a different set of parties. We prove here that much stronger monogamy relations are possible for nonsignaling correlations by showing how nonlocal correlations...

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• Lobular carcinoma, NOS - Female, 66 - Tissue image [6260730021881071]

  Open Research Data

  open access

  • Gdański Uniwersytet Medyczny

  This is the histopathological image of BREAST tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.

• Lobular carcinoma, NOS - Female, 66 - Tissue image [6260730021881931]

  Open Research Data

  open access

  • Gdański Uniwersytet Medyczny

  This is the histopathological image of BREAST tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.

• Lobular carcinoma, NOS - Female, 66 - Tissue image [6260730021885831]

  Open Research Data

  open access

  • Gdański Uniwersytet Medyczny

  This is the histopathological image of BREAST tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.

• Micropapillary carcinoma of breast - Female, 57 - Tissue image [10130730023084341]

  Open Research Data

  open access

  • Gdański Uniwersytet Medyczny

  This is the histopathological image of BREAST tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.

• Micropapillary carcinoma of breast - Female, 57 - Tissue image [10130730023087991]

  Open Research Data

  open access

  • Gdański Uniwersytet Medyczny

  This is the histopathological image of BREAST tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.

• Biofilm formation and its regulation in Staphylococcus aureus

  Publication

  • M. Schielmann

  - Year 2013

  Infections caused by the major human and animal pathogen, Staphylococcus aureus are becoming an important problem due to its emerging resistance mechanisms and variety of virulence factors. Biofilm formation is considered to be one of the most important factor attributed to high pathogenicity of S. aureus. Described in the literature methods used in identification of biofilm forming abilities of bacteria consist of phenotypic and...

• No Quantum Realization of Extremal No-Signaling Boxes

  Publication

  • R. Ramanathan
  • J. Tuziemski
  • M. Horodecki
  • P. Horodecki

  - PHYSICAL REVIEW LETTERS - Year 2016

  The study of quantum correlations is important for fundamental reasons as well as for quantum communication and information processing tasks. On the one hand, it is of tremendous interest to derive the correlations produced by measurements on separated composite quantum systems from within the set of all correlations obeying the no-signaling principle of relativity, by means of information-theoretic principles. On the other hand,...

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• Stardust - Investigation of Microbes in the Stratosphere

  Publication

  • M. Jasiukowicz
  • B. Rybacki
  • M. Grzybowski
  • N. Czortek
  • D. Rekowski
  • J. Pawłowski
  • P. Podpirko
  • K. Loret
  • D. Tomaszewska
  • S. Magrian... and 4 others

  - Year 2022

  The stratospheric microbiome has been investigated several times using the methods of classical microbiology. In this experiment, we have combined them with some novel approaches including whole- metagenome amplification, Maldi TOF mass spectrometry and Sanger DNA sequencing. The results of the experiment may provide the scientists with knowledge about the mechanisms of survivability of microorganisms in stratospheric conditions...

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• Invasive breast carcinoma of no special type - Female, 58 - Tissue image [6180730013791631]

  Open Research Data

  open access

  • Gdański Uniwersytet Medyczny

  This is the histopathological image of BREAST tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.

• Mucinous carcinoma - Female, 73 - Tissue image [3150630013128701]

  Open Research Data

  open access

  • Gdański Uniwersytet Medyczny

  This is the histopathological image of BREAST tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.

• Lobular carcinoma, NOS - Female, 66 - Tissue image [6260730021888051]

  Open Research Data

  open access

  • Gdański Uniwersytet Medyczny

  This is the histopathological image of BREAST tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.

• Mucinous carcinoma - Female, 73 - Tissue image [315063001312661]

  Open Research Data

  open access

  • Gdański Uniwersytet Medyczny

  This is the histopathological image of BREAST tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.

• Lobular carcinoma, NOS - Female, 66 - Tissue image [6260730021886431]

  Open Research Data

  open access

  • Gdański Uniwersytet Medyczny

  This is the histopathological image of BREAST tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.

• Mucinous carcinoma - Female, 73 - Tissue image [3150630013121201]

  Open Research Data

  open access

  • Gdański Uniwersytet Medyczny

  This is the histopathological image of BREAST tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.

• Mucinous carcinoma - Female, 73 - Tissue image [3300730069471511]

  Open Research Data

  open access

  • Gdański Uniwersytet Medyczny

  This is the histopathological image of BREAST tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.

• Lobular carcinoma, NOS - Female, 41 - Tissue image [6270730022323671]

  Open Research Data

  open access

  • Gdański Uniwersytet Medyczny

  This is the histopathological image of BREAST tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.

• Lobular carcinoma, NOS - Female, 41 - Tissue image [6270730022321301]

  Open Research Data

  open access

  • Gdański Uniwersytet Medyczny

  This is the histopathological image of BREAST tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.

• Invasive breast carcinoma of no special type - Female, 58 - Tissue image [618073001379851]

  Open Research Data

  open access

  • Gdański Uniwersytet Medyczny

  This is the histopathological image of BREAST tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.

• Invasive breast carcinoma of no special type - Female, 58 - Tissue image [618073001379291]

  Open Research Data

  open access

  • Gdański Uniwersytet Medyczny

  This is the histopathological image of BREAST tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.

• Invasive breast carcinoma of no special type - Female, 58 - Tissue image [61807300137911]

  Open Research Data

  open access

  • Gdański Uniwersytet Medyczny

  This is the histopathological image of BREAST tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.

• Invasive breast carcinoma of no special type - Female, 58 - Tissue image [6180730013795811]

  Open Research Data

  open access

  • Gdański Uniwersytet Medyczny

  This is the histopathological image of BREAST tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.

• Mucinous carcinoma - Female, 73 - Tissue image [3150630013121491]

  Open Research Data

  open access

  • Gdański Uniwersytet Medyczny

  This is the histopathological image of BREAST tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.

• Lobular carcinoma, NOS - Female, 41 - Tissue image [6270730022329521]

  Open Research Data

  open access

  • Gdański Uniwersytet Medyczny

  This is the histopathological image of BREAST tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.

• Lobular carcinoma, NOS - Female, 41 - Tissue image [6270730022321451]

  Open Research Data

  open access

  • Gdański Uniwersytet Medyczny

  This is the histopathological image of BREAST tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.

• Lobular carcinoma, NOS - Female, 41 - Tissue image [6270730022323751]

  Open Research Data

  open access

  • Gdański Uniwersytet Medyczny

  This is the histopathological image of BREAST tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.

• Lobular carcinoma, NOS - Female, 41 - Tissue image [6270730022317671]

  Open Research Data

  open access

  • Gdański Uniwersytet Medyczny

  This is the histopathological image of BREAST tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.

• Wind Conditions at Pedestrian Level in Different Types of Residential Urban Development for a High Degree of Land Use Efficiency

  Publication

  • K. Zielonko-Jung
  • M. Poćwierz
  • R. Idem

  - Sustainability - Year 2021

  The paper concerns wind conditions around urban building development at the pedestrian level. The article aims to identify aerodynamic phenomena around three types of multi-family housing developmentswith different forms and the same urban parameters of building development intensity (high intensity was taken into account). The aim of the research was mainly to achieve qualitative results that would lead to understanding fundamental...

  Full text available to download

• Lobular carcinoma, NOS - Female, 64 - Tissue image [3200630011577501]

  Open Research Data

  open access

  • Gdański Uniwersytet Medyczny

  This is the histopathological image of BREAST tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.

• Lobular carcinoma, NOS - Female, 64 - Tissue image [3200630011575701]

  Open Research Data

  open access

  • Gdański Uniwersytet Medyczny

  This is the histopathological image of BREAST tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.

• Tubular carcinoma - Female, 65 - Tissue image [6270730022325571]

  Open Research Data

  open access

  • Gdański Uniwersytet Medyczny

  This is the histopathological image of BREAST tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.

• Tubular carcinoma - Female, 65 - Tissue image [6270730022322841]

  Open Research Data

  open access

  • Gdański Uniwersytet Medyczny

  This is the histopathological image of BREAST tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.

• Lobular carcinoma, NOS - Female, 64 - Tissue image [3200630011577991]

  Open Research Data

  open access

  • Gdański Uniwersytet Medyczny

  This is the histopathological image of BREAST tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.

• Tubular carcinoma - Female, 65 - Tissue image [6270730022323831]

  Open Research Data

  open access

  • Gdański Uniwersytet Medyczny

  This is the histopathological image of BREAST tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.

• Lobular carcinoma, NOS - Female, 64 - Tissue image [3200630011577381]

  Open Research Data

  open access

  • Gdański Uniwersytet Medyczny

  This is the histopathological image of BREAST tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.

• Infiltrating lobular carcinoma, NOS - Female, 60 - Tissue image [8160730027009471]

  Open Research Data

  open access

  • Gdański Uniwersytet Medyczny

  This is the histopathological image of BREAST tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.

• Lobular carcinoma, NOS - Female, 64 - Tissue image [3200630011579731]

  Open Research Data

  open access

  • Gdański Uniwersytet Medyczny

  This is the histopathological image of BREAST tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.

• Lobular carcinoma, NOS - Female, 64 - Tissue image [3200630011579231]

  Open Research Data

  open access

  • Gdański Uniwersytet Medyczny

  This is the histopathological image of BREAST tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.

• Tubular carcinoma - Female, 65 - Tissue image [6270730022325041]

  Open Research Data

  open access

  • Gdański Uniwersytet Medyczny

  This is the histopathological image of BREAST tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.