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Search results for: restriction digest analysis
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DNA restriction analysis as a supportive tool in mechanistic studies carried out by 32P-postlabelling
PublicationNumerous antitumor and carcinogenic compounds are able to modify DNA by forming covalent bonds with its constituents, while some anticarcinogenic compounds are known to prevent such a modification. All these processes are of vital biological import_ance, though deeper inside into factors influencing formation of DNA adducts is difficult due to the low level of their occurrence. 32P-Postlabelling approach ensures very sensitive...
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The extended version of restriction analysis approach for the examination of the ability of low-molecular-weight compounds to modify DNA in a cell-free system
PublicationOne of the primary requirements in toxicology is the assessment of ability of chemicals to induce DNA covalent modification. There are several well-established methods used for this purpose such as 32P-Postlabeling or HPLC-MS. However, all of these approaches have difficult to overcome limitations, which prevents their use in genotoxin screening. Here, we describe the simple protocol exploiting specificity of restriction enzymes...
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DNA modification by 1-nitroacridine derivatives. Comparison of 32P-Postlabeling and restriction enzyme analysis method for the detection of DNA adducts formed by 1-nitroacridines C-1748 and C-857
Publication4-podstawione 1-nitroakrydyny reprezentują nową grupę pochodnych akrydyny, zsyntetyzowanych na Politechnice Gdańskiej. Metoda 32P-postlabellingu wykazała, że pochodne C-1748 i C-857 tworzą od 4-6 adduktów DNA w systemie komórkowym i bezkomórkowym o innym rozkładzie plam chromatograficznych, jednakże niezależnym od zastosowanego systemu aktywacyjnego. Metoda enzymatycznej analizy restrykcyjnej pozwoliła natomiast śledzić kinetykę...
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Milena Marycz dr inż.
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Czynniki rozwojowe w dokumentach strategicznych miast powiatowych na obszarze peryferyjnym województwa pomorskiego
PublicationAutor postawił dwa cele: 1) zweryfikowanie hipotezy, że każde terytorium posiada endogeniczny potencjał rozwojowy; 2) sprawdzenie, czy sposób rozpoznawania i analizy czynników rozwojowych są odpowiednie dla sformułowanie właściwej strategii. Autor badał czynniki rozwojowe miast powiatowych ze względu na ich rolę w aktywizacji obszarów peryferyjnych (czyli poza obszarem metropolitalnym). Głównym źródłem informacji były elementy...
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Optical Fibre Technology - A Digest
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Development of particle accelerator technology in Europe: digest of infrastructural and research projects
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The New LM-PCR/Shifter Method for the Genotyping of Microorganisms Based on the Use of a Class IIS Restriction Enzyme and Ligation Mediated PCR
PublicationThis study details and examines a novel Ligation-Mediated - Polymerase Chain Reaction (LM-PCR) method. Named the LM-PCR/Shifter, it relies on the use of a Class IIS restriction enzyme giving restriction fragments with different 4 base, 5' overhangs, this being the Shifter, and the ligation of appropriate oligonucleotide adapters. A sequence of 4-base, 5' overhangs of the adapter and a 4-base sequence of the 3' end of the primer(s)...
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SAAD digest
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Characterization of restriction endonucleaseAjoI fromAcinetobacter johnsonii
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Błażej Prusak dr hab.
PeopleBłażej Prusak is Head of the Department of Finance at the Faculty of Management and Economics, Gdansk University of Technology and Editor-in-Chief of the journal Research on Enterprise in Modern Economy - theory and practice (REME), as well as a member of editorial boards of such journals as Intellectual Economics; Space. Economics. Society; Academy of Management. He is the author or co-author of several scientific monographs including:...
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ENGINEERING FAILURE ANALYSIS
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A simple modification to improve the accuracy of methylation-sensitive restriction enzyme quantitative polymerase chain reaction
PublicationDNA digestion with endonucleases sensitive to CpG methylation such as HpaII followed by polymerase chain reaction (PCR) quantitation is commonly used in molecular studies as a simple and inexpensive solution for assessment of region-specific DNA methylation. We observed that the results of such analyses were highly overestimated if mock-digested samples were applied as the reference.We determined DNA methylation levels in several...
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Financial Analysis exercises - New
e-Learning CoursesTo obtain theoretical and practical experience of financial and economic analysis. Methods of economic analysis: Elements of production and human resources analysis, tangible assets analysis, inventory analysis, which would enable to understand the business case, to measure them and find suitable decision. To obtain theoretical and practical experience of financial analysis.
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A new assay based on terminal restriction fragment length polymorphism of homocitrate synthase gene fragments for Candida species identification
PublicationCandida sp. have been responsible for an increasing number of infections, especially in patients with immunodeficiency. Species specific differentiation of the Candida sp. is difficult in routine diagnosis. This identification can have a highly significant association in therapy and prophylaxis. This work has shown a new application of terminal restriction fragment length polymorphism (t-RFLP) method in molecular identification...
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15th Conference on Calorimetry and Thermal Analysis
EventsRegistration for the workshops with Sponsors, which will take place during the free time on Tuesday, September 10, 2024, from 2:30 PM, will be conducted by signing up on a list at the registration desk. We kindly inform you that posters should be prepared in size B1 (707 x 1000 mm). Templates:pptx, pdf Please submit your presentations...
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Isolation and characterization of the restriction endonucleasePpel fromPhormidium persicinum
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Financial Analysis exercises - New (copy)
e-Learning CoursesTo obtain theoretical and practical experience of financial and economic analysis. Methods of economic analysis: Elements of production and human resources analysis, tangible assets analysis, inventory analysis, which would enable to understand the business case, to measure them and find suitable decision. To obtain theoretical and practical experience of financial analysis.
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Decisions Analysis 2023/2024
e-Learning CoursesThis is content to the subject: "Decisions Analysis" to be used as a template for futher courses.
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Qualitative data analysis methods
e-Learning CoursesThis is the continuation of Qualitatative Data Analysis Methods course provided online
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Water and Wastes Digest
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Agricultural Science Digest
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Decisions Analysis STAC 22/23
e-Learning CoursesThis is content to the subject: "Decisions Analysis" 22/23
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TspGWI, a thermophilic class-IIS restriction endonuclease from Thermus sp.,recognizes novel asymmetric sequence 5´-ACGGA(N11/9)-3
PublicationA novel prototype class-IIS restriction endonuclease, TspGWI, was isolated from the thermophilic bacterium Thermus sp. GW. The recognition sequence and cleavage positions have been established: TspGWI recognizes the non-palindromic 5-bp sequence 5′-ACGGA-3′ and cleaves the DNA 11 and 9 nt downstream in the top and bottom strand, respectively. In addition, an accompanying endonuclease, TspGWII, an isoschizomer of Pst I, was found...
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Platelets in fetal growth restriction: role of reactive oxygen species, oxygen metabolism, and aggregation.
PublicationFetal growth restriction (FGR) is mainly caused by failure of the uteroplacental unit. Theexact pathogenesis remains unclear. The cause is thought to be related to abnormal platelet activation,which may...
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Data Analysis 2023/24
e-Learning CoursesData Analysisdr inż. Karol Flisikowski, prof. PG - winter semester 2023/24
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Macroeconomic analysis
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Market analysis
e-Learning CoursesKurs przeznaczony dla studentów studiów stacjonarnych Inżynierii Danych, I stopnia, semestr 7 (zimowy) w roku akademickim 2021/2022.
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Financial Analysis
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Decision analysis
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Digest Journal of Nanomaterials and Biostructures
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Digest of Middle East Studies
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Isolation and identification of the restriction endonuclease Ptal fromPhormidium tadzschicicum, an isoschizomer of BspMII
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The altered platelet mineral ratios in pregnancy complicated with intrauterine growth restriction
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Resistance Training with Blood Flow Restriction and Ocular Health: A Brief Review
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Novel application of terminal restriction fragments length polymorphism method (t-RFLP) for identification of six clinically important Candida sp.
PublicationTerminal restriction fragments length polymorphism method (t-RFLP) is a modified classical RFLP method. The main difference is based on carrying out PCR using a fluorescently labeled primer or primers. The result of such modification is that after digestion of amplicons only terminal fragments of PCR product (containing fluorescent dye) are visualized. T-RFLP method has found application in environmental microbiology and epidemiology....
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Decision analysis (l/lab) winter 2020/21
e-Learning CoursesDecision analysis (l/lab)_winter 2020/21
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Deep neural networks for data analysis
e-Learning CoursesThe aim of the course is to familiarize students with the methods of deep learning for advanced data analysis. Typical areas of application of these types of methods include: image classification, speech recognition and natural language understanding. Celem przedmiotu jest zapoznanie studentów z metodami głębokiego uczenia maszynowego na potrzeby zaawansowanej analizy danych. Do typowych obszarów zastosowań tego typu metod należą:...
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Advanced OCT techniques for biomedical applications - theoretical ideas versus technical restriction
PublicationW pracy przedstawiono dyskusję nad możliwościami pomiarowymi współczesnych systemów OCT o różnych konfiguracjach ( w tym o konfiguracji Mach-Zehndera i Michelsona) pracujących w dziedzinie czasu lub częstotliwości. Przedstawiono między innymi konfiguracje czułe na stan polaryzacji, na ruch w wykonaniu światłowodowym lub objętościowym.
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METHODS OF QUANTITATIVE ANALYSIS (TEAM PROJECT) (2022/2023)
e-Learning CoursesMETHODS OF QUANTITATIVE ANALYSIS (TEAM PROJECT) - Economic Analytics, Lecture and Lab, Winter Semester 2022/2022. Teacher: dr Piotr Paradowski
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Selected aspects of modern X-ray structural analysis 2024
e-Learning CoursesSelected Aspects of Modern X-ray Structural Analysis
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Selected Aspects of Modern X-ray Structural Analysis 2023
e-Learning CoursesSelected Aspects of Modern X-ray Structural Analysis
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Fundamentals of microscopic analysis
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Human Resource Management International Digest
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JOHNS HOPKINS APL TECHNICAL DIGEST
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Analysis
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ESAIM: Mathematical Modelling and Numerical Analysis
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Acute Effects of Different Blood Flow Restriction Protocols on Bar Velocity During the Squat Exercise
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Acute impact of blood flow restriction on strength-endurance performance during the bench press exercise
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PHYTOCHEMICAL ANALYSIS
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