A method for a comprehensive lipidomic analysis of flaxseed (Linum usitatissimum) with the use of LC–Q-TOF-MS and dispersive micro-solid-phase (μDSPE) extraction
Abstrakt
Flaxseed (FS) is one of the richest sources of α-linolenic acid oil and lignans, and it is suggested that the consumption of flaxseed may contribute to the prevention of certain chronic diseases such as many types of cancer, diabetes, cardiovascular diseases and cerebrovascular stroke. Here, we demonstrate a new method for comprehensive FS lipidome profiling with the use of LC–Q-TOF-MS and dispersive micro-solid-phase extraction. The effects of stationary phase amount, flaxseed amount and different organic solvents for non-polar lipid elution on the FS lipidome coverage were investigated. The developed and validated protocol allowed for improved monitoring of both polar and non-polar lipids simultaneously, overcoming the challenge of low- and high-abundance lipid species. Furthermore, the method was applied to characterize a set of brown flaxseed and yellow flaxseed samples, as well as flaxseed meal.
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Informacje szczegółowe
- Kategoria:
- Publikacja w czasopiśmie
- Typ:
- artykuły w czasopismach
- Opublikowano w:
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FOOD CHEMISTRY
nr 381,
ISSN: 0308-8146 - Język:
- angielski
- Rok wydania:
- 2022
- Opis bibliograficzny:
- Bakhytkyzy I., Hewelt-Belka W., Kot-Wasik A.: A method for a comprehensive lipidomic analysis of flaxseed (Linum usitatissimum) with the use of LC–Q-TOF-MS and dispersive micro-solid-phase (μDSPE) extraction// FOOD CHEMISTRY -Vol. 381, (2022), s.132290-
- DOI:
- Cyfrowy identyfikator dokumentu elektronicznego (otwiera się w nowej karcie) 10.1016/j.foodchem.2022.132290
- Źródła finansowania:
-
- Publikacja bezkosztowa
- Weryfikacja:
- Politechnika Gdańska
wyświetlono 503 razy
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