Construction of glucosamine-6-phosphate synthase with an internal hexahistydyl fragment

Karolina Kwiatkowska; Justyna Czarnecka

Construction of glucosamine-6-phosphate synthase with an internal hexahistydyl fragment

Abstrakt

L-Glutamine: D-fructose-6-phosphate aminotransferase (EC 2.6.1.16), known also as glucosamine-6-phosphate (GlcN-6-P) syntase, catalyzes the first committed step in the amino sugar biosynthetic pathway in prokaryotic and eukaryotic organisms. The enzyme carries out a complex reaction involving ammonia transfer and sugar phosphate isomerization. GlcN-6-P syntase is an important point of metabolic control of amino sugar biosynthesis and postulated a putative target for antifungal chemotherapy. The enzyme is well known for its relative instability and difficulties in purification. One of the possible approaches to facilitate enzyme purification is a construction of its hexaHistag-containing version that can be easily isolated from complex mixtures by the one-step metal affinity chromatography. Unfortunately, introduction of hexahistydyl tags at either N- or C-terminus of GlcN-6-P synthase results in lost of the enzyme activity.

Autorzy (2)

Karolina Kwiatkowska
Justyna Czarnecka

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Kategoria:: Aktywność konferencyjna
Typ:: publikacja w wydawnictwie zbiorowym recenzowanym (także w materiałach konferencyjnych)
Tytuł wydania:: 13th International Symposium of Students and Young Mechanical Engineers : Advances in Chemical and Mechanical Engineering, Gdańsk, 20th-22nd May 2010 strony 216 - 217
Język:: polski
Rok wydania:: 2010
Opis bibliograficzny:: Kwiatkowska K., Czarnecka J.: Construction of glucosamine-6-phosphate synthase with an internal hexahistydyl fragment// 13th International Symposium of Students and Young Mechanical Engineers : Advances in Chemical and Mechanical Engineering, Gdańsk, 20th-22nd May 2010/ ed. ed. C. Fijało. - Gdansk University of Technology. Gdańsk: GUT, 2010, s.216-217
Weryfikacja:: Politechnika Gdańska