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An MTA-phosphorylase gene discovered in the metagenomic library derived from Antarctic top soil during screening for lipolytic active clones confers strong pink fluorescence in the presence of rhodamine B.
PublikacjaIn this work, we present the construction of a metagenomic library in Escherichiacoli using the pUC19 vector and environmental DNA directly isolated fromAntarctic topsoil and screened for lipolytic enzymes. Unexpectedly, the screeningon agar supplemented with olive oil and rhodamine B revealed one unusual pinkfluorescent clone (PINKuv) out of 85 000 clones. This clone harbored a plasmid,pPINKuv, which has an insert of 8317 bp that...
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Regulated Proenkephalin Expression in Human Skin and Cultured Skin Cells
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CD73 Expression as a Potential Marker of Good Prognosis in Breast Carcinoma
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Nicotinamide phosphoribosyltransferase expression in thyroid glands of patients with Graves' disease
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Comprehensive analysis of microRNA expression profile in malignant glioma tissues
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A new B-D-galactosidase with a low temperature optimum isolated from the Antarctic Arthrobacter sp. 20B: gene cloning, purification and characterization.
PublikacjaA psychrotrophic bacterium producing a coldadaptedB-galactosidase upon growth at low temperatureswas classiWed as Arthrobacter sp. 20B. A genomic DNAlibrary of strain 20B introduced into Escherichia coliTOP10F' and screening on X-Gal (5-bromo-4-chloro-3-indolyl-B-D-galactopyranoside)-containing agar plates ledto the isolation of B-galactosidase gene. The B-galactosidasegene (bgaS) encoding a protein of 1,053 amino acids,with a...
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Genes Involved in the Processes of Cell Proliferation, Migration, Adhesion, and Tissue Development as New Potential Markers of Porcine Granulosa Cellular Processes In Vitro: A Microarray Approach
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Ion homeostasis and transport are regulated by genes differentially expressed in porcine buccal pouch mucosal cells during long-term culture in vitro – a microarray approach
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Cloning, expression, and biochemical characterization of a coldactive GDSL-esterase of a Pseudomonas sp. S9 isolated from Spitsbergen island soil
PublikacjaAn estS9 gene, encoding an esterase of the psychrotolerant bacterium Pseudomonas sp. S9 was cloned and sequenced. The deduced sequence revealed a protein of 636 amino acid residues with a molecular mass of 69 kDa.Further amino acid sequence analysis revealed that the EstS9 enzyme contained a G-D-S-L motif centered at a catalytic serine, an N-terminal catalytic domain and a C-terminal autotransporter domain. Two recombinant E. coli...
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Relationship between polymorphism in the CRH gene and the traits of body conformation of Salers cows
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Association of ENAM gene single nucleotide polymorphisms with dental caries in Polish children
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Proteins Structure Models in the Evaluation of Novel Variant (C.472_477del) in the MOCS2 Gene
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Prognostic Significance of TOP2A Gene Dosage in HER-2-Negative Breast Cancer
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Germline mutations in theCHEK2 kinase gene are associated with an increased risk of bladder cancer
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Gene set enrichment for reproducible science: comparison of CERNO and eight other algorithms
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Research Trends of Vitamin D Metabolism Gene Polymorphisms Based on a Bibliometric Investigation
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The Relationship Between Gene Polymorphisms and Dipping Profile in Patients With Coronary Heart Disease
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Cloning of the thermostable alpha-amylase gene from Pyrococcus woesei in Escherichia coli.
PublikacjaGen kodujący alfa-amylazę z Pyrococcus woesei klonowano do plazmidów pET21d(+)lub pYTB2, którymi transformowano komórki Escherichia coli. Otrzymana rekombinantowa alfa amylaza wykazuje maksymalną aktywność przy pH 5.6 w temperaturze 95 stC i przejawia około 24 % początkowej aktywności nawet po 2 godz. inkubacji w 120 stC. Duża termostabilność otrzymanego preparatu świadczy o jego przydatności do enzymatycznego upłynniania skrobi.
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Image based analysis of emotional expression development in facial transplant patient
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The significance of lumican expression in ovarian cancer drug-resistant cell lines
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The significance of caveolin-1 expression in parietal epithelial cells of Bowman’s capsule
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Expression of intermediate filaments of podocytes within nephrotic syndrome glomerulopathies in children
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The effect of cabbage juices on the activity and expression of GST isozymes in HepG2 cells
PublikacjaWcześniejsze badania pokazały zdolność soków z kapusty białej do indukowania aktywności enzymów detoksykacyjnych. Prezentowane wyniki potwierdziły zdolność fitokompleksu kapusty do stymulacji aktywności enzymów z grupy GST w komórkach HepG2 - modelu ludzkiej wątroby - w szczególności izoenzymów GST-alfa i GST-mi.
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Clinical Implications of mTOR Expression in Papillary Thyroid Cancer—A Systematic Review
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Modulation of expression of genes involved in glycosaminoglycan metabolism and lysosome biogenesis by flavonoids
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Expression of Selected Epithelial-Mesenchymal Transition Transcription Factors in Endometrial Cancer
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Increased expression of PELP1 in human sperm is correlated with decreased semen quality
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Expression of ghrelin and ghrelin functional receptor GHSR1a in human pituitary adenomas
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TOLLIP Protein Expression Predicts Unfavorable Outcome in Renal Cell Carcinoma
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Association between uridin diphosphate glucuronosylotranserase 1A1 (UGT1A1) gene polymorphism and neonatal hyperbilirubinemia
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Liver steatosis correlates with iron overload but not with HFE gene mutations in chronic hepatitis C
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Associations of ESR1 and ESR2 gene polymorphisms with metabolic syndrome and its components in postmenopausal women
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Prognostic Value of TOP2A Gene Amplification and Chromosome 17 Polysomy in Early Breast Cancer
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Sensitivity, Specificity and Prioritization of Gene Set Analysis When Applying Different Ranking Metrics
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TGF-β gene polimorphisms as risk factors for asthma control among clinic patients
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TGF-β gene polimorphisms as risk factors for asthma control among clinic patients.
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TGF-β gene polimorphisms as risk factors for asthma control among clinic patients.
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Gene Therapy Approaches to Prevent Corneal Graft Rejection: Where Do We Stand?
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Genetic and structural characterization of the growth hormone gene and protein from tench, Tinca tinca
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Graphene-encapsulated iron nanoparticles as a non-viral vector for gene delivery into melanoma cells
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The effect of sunscreen 4‐methylbenzylidene camphor in different and reproductive models, its bioaccumulation and molecular effects on ligand‐receptor interaction, and protein expression
Publikacja4-Methylbenzylidene camphor (4-MBC) is a photo-absorbing UV filter prevalently used in cosmetics, which can be absorbed into circulation and cause systemic effects. 4-MBC is continued to be released in the environment despite the growing knowledge about its bioaccumulation and endocrine disrupting effects. Previous reviews have mentioned UV-filter together but this review considers 4-MBC alone, due to its prevalence and concerning...
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Closed-form expression for the magnetic shielding constant of the relativistic hydrogenlike atom in an arbitrary discrete energy eigenstate: Application of the Sturmian expansion of the generalized Dirac–Coulomb Green function
PublikacjaWe present analytical derivation of the closed-form expression for the dipole magnetic shielding constant of a Dirac one-electron atom being in an arbitrary discrete energy eigenstate. The external magnetic field, by which the atomic state is perturbed, is assumed to be weak, uniform, and time independent. With respect to the atomic nucleus we assume that it is pointlike, spinless, motionless, and of charge Ze. Calculations are...
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Characteristics of the peanut chain in Europe – implications for peanut allergy
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Analysis of polymorphism and hepatic expression of duodenal cytochrome b in chronic hepatitis C
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Altered circadian genes expression in breast cancer tissue according to the clinical characteristics
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Stromal expression of ALDH1 in human breast carcinomas indicates reduced tumor progression
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Expression of estrogen receptors, PELP1, and SRC in human spermatozoa and their associations with semen quality
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Brain-Inspired Deep Networks for Facial Expression Recognition. Frontiers in Biomedical Technologies
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Differential Expression of HIF1A, EPAS1, and VEGF Genes in Benign and Malignant Ovarian Neoplasia
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Changes in the Microrna Expression Profile in Patients Undergoing Autologous Steam Cell Transplantation
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