Wyszukiwarka
Wyniki wyszukiwania dla: ANTYGENY REKOMBINANTOWE, BORELIOZA, BORRELIA BURGDORFERI SENSU LATO, IMMUNODIAGNOSTYKA
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ANTYGENY REKOMBINANTOWE W DIAGNOSTYCE SEROLOGICZNEJ BORELIOZY
PublikacjaBorelioza jest najczęstszą chorobą odkleszczową dotykającą mieszkańców półkuli północnej. Chorobę tę wywołują bakterie zakwalifikowane do grupy Borrelia burgdorferi sensu lato. Współcześnie podstawą diagnostyki laboratoryjnej boreliozy jest dwustopniowe badanie serologiczne. Pierwszym etapem jest test immunoenzymatyczny (ELISA), jeżeli wynik badania jest dodatni lub wątpliwy jako test potwierdzający stosuje się technikę Western...
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PRZYDATNOŚĆ DIAGNOSTYCZNA REAKCJI PCR W ROZPOZNAWANIU BORELIOZY
PublikacjaBorelioza jest wieloukładową chorobą wywoływaną przez bakterie zaliczane do kompleksu Borrelia burgdorferi sensu lato. Wektorem przenoszącym infekcję są kleszcze z rodzaju Ixodes, które zakażają kolejnych żywicieli krętka podczas spożywania ich krwi. Zróżnicowany przebieg boreliozy uniemożliwia jej rozpoznanie na podstawie objawów klinicznych. W związku z tym diagnostyka choroby z Lyme opiera się głównie na metodach laboratoryjnych,...
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Novel monovalent and multivalent recombinant proteins of Borrelia burgdorferi sensu lato with potential diagnostic value – construction and biotechnological production
PublikacjaThe diverse antigenic structure of Borrelia burgdorferi sensu lato (s.l.) and the low degree of protein sequence conservation between genospecies causes many limitations in serodiagnosis of Lyme disease (LD). Using expression systems based on Escherichia coli, five monovalent B. burgdorferi s.l. recombinant proteins were produced. i.e., BB0108, BB0126, BB0298, BB0323, BB0689 (each in three variants derived from Borrelia afzelii,...
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New BB0108, BB0126, BB0298, BB0323, and BB0689 Chromosomally Encoded Recombinant Proteins of Borrelia burgdorferi sensu lato for Serodiagnosis of Lyme Disease
PublikacjaFive chromosomally encoded proteins, BB0108, BB0126, BB0298, BB0323, and BB0689, from Borrelia burgdorferi sensu lato (s.l.), were obtained in three variants each, representing the most common genospecies found in Europe (Borrelia afzelii, Borrelia burgdorferi sensu stricto (s.s.), and Borrelia garinii). The reactivity of these recombinant proteins with the IgM and IgG antibodies present in human serum was assessed using Western...
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Nowe monowalentne i multiwalentne rekombinowane białka Borrelia burgdorferi sensu lato jako potencjalne antygeny do wykrywania swoistych przeciwciał w surowicach pacjentów z boreliozą
ProjektyProjekt realizowany w Katedra Biotechnologii Molekularnej i Mikrobiologii zgodnie z porozumieniem UMO-2023/49/B/NZ6/02881 z dnia 2024-03-01
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In silico epitope prediction of Borrelia burgdorferi sensu lato antigens for the detection of specific antibodies
PublikacjaDespite many years of research, serodiagnosis of Lyme disease still faces many obstacles. Difficulties arise mainly due to the low degree of amino acid sequence conservation of the most immunogenic antigens among B. burgdorferi s.l. genospecies, as well as differences in protein production depending on the environment in which the spirochete is located. Mapping B-cell epitopes located on antigens allows for a better understanding...
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Epitope Mapping of BmpA and BBK32 Borrelia burgdorferi Sensu Stricto Antigens for the Design of Chimeric Proteins with Potential Diagnostic Value
PublikacjaLyme disease is a tick-borne zoonosis caused by Gramnegative bacteria belonging to the Borrelia burgdorferi sensu lato (s.l.) group. In this study, IgM- and IgG-specific linear epitopes of two B. burgdorferi sensu stricto (s.s.) antigens BmpA and BBK32 were mapped using a polypeptide array. Subsequently, two chimeric proteins BmpABBK32-M and BmpA-BBK32-G were designed to validate the construction of chimeras using the identified...
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Borrelia burgdorferi BmpA-BBK32 and BmpA-BBA64: New Recombinant Chimeric Proteins with Potential Diagnostic Value
PublikacjaCurrently, the diagnosis of Lyme disease is based mostly on two-tiered serologic testing. In the new generation of immunoenzymatic assays, antigens comprise whole-cell lysates of members of the Borrelia burgdorferi sensu lato (s.l.) species complex, with the addition of selected recombinant proteins. Due to the high diversity of members of the B. burgdorferi s.l. genospecies and the low degree of conservation among the amino acid...
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Anxiety and depression in patients infected with Borrelia burgdorferi
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Antygeny rekombinantowe Toxoplasma gondii jako narzędzie w diagnostyce toksoplazmozy
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Variability in feeding habitats of red deer sensu lato in Eurasia in the Late Pleistocene and Holocene
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Szczepionki DNA i antygeny rekombinantowe w prewencji zarażeń Toxoplasma gondii - aktualny stan badań
PublikacjaToksoplazmoza wywoływana przez wewnątrzkomórkowego pasożyta Toxoplasma gondii jest jedną z najbardziej rozpowszechnionych odzwierzęcych chorób na świecie o bardzo dużym znaczeniu medycznym. Stanowi ponadto bardzo poważny problem weterynaryjny, ponieważ jest główną przyczyną strat reprodukcyjnych w hodowli zwierząt (głównie owiec). Z tego względu bardzo ważne są prace dotyczące konstrukcji i opracowania nowych, skutecznych i tanich...
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Construction of recombinant plasmid coding Borrelia burgdorferi sl B1 protein
Dane BadawczeDiagnosis of Lyme borreliosis (LB), an infection caused by spirochaetes of the B. burgdorferi sl species complex, is mainly based on clinical symptoms supported with serology and is often misdiagnosed in areas of endemicity. The complexity of the antigenic composition among the Borrelia genospecies and differential expression of proteins in host and...
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Construction of recombinant plasmid coding Borrelia burgdorferi sl B6 protein
Dane BadawczeDiagnosis of Lyme borreliosis (LB), an infection caused by spirochaetes of the B. burgdorferi sl species complex, is mainly based on clinical symptoms supported with serology and is often misdiagnosed in areas of endemicity. The complexity of the antigenic composition among the Borrelia genospecies and differential expression of proteins in host and...
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Construction of recombinant plasmid coding Borrelia burgdorferi sl B2 protein
Dane BadawczeDiagnosis of Lyme borreliosis (LB), an infection caused by spirochaetes of the B. burgdorferi sl species complex, is mainly based on clinical symptoms supported with serology and is often misdiagnosed in areas of endemicity. The complexity of the antigenic composition among the Borrelia genospecies and differential expression of proteins in host and...
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Construction of recombinant plasmid coding Borrelia burgdorferi sl B12 protein
Dane BadawczeDiagnosis of Lyme borreliosis (LB), an infection caused by spirochaetes of the B. burgdorferi sl species complex, is mainly based on clinical symptoms supported with serology and is often misdiagnosed in areas of endemicity. The complexity of the antigenic composition among the Borrelia genospecies and differential expression of proteins in host and...
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Construction of recombinant plasmid coding Borrelia burgdorferi sl B3 protein
Dane BadawczeDiagnosis of Lyme borreliosis (LB), an infection caused by spirochaetes of the B. burgdorferi sl species complex, is mainly based on clinical symptoms supported with serology and is often misdiagnosed in areas of endemicity. The complexity of the antigenic composition among the Borrelia genospecies and differential expression of proteins in host and...
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Construction of recombinant plasmid coding Borrelia burgdorferi sl P protein
Dane BadawczeDiagnosis of Lyme borreliosis (LB), an infection caused by spirochaetes of the B. burgdorferi sl species complex, is mainly based on clinical symptoms supported with serology and is often misdiagnosed in areas of endemicity. The complexity of the antigenic composition among the Borrelia genospecies and differential expression of proteins in host and...
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Biotechnological production and purification of Borrelia burgdorferi sl B1 protein
Dane BadawczeBorrelia burgdorferi B1 protein could be used in the detection of specific immunoglobulins from patients' sera with Lyme disease that may be infected with different genospecies of B. burgdorferii sl. The goal of the study was to obtain the highest possible yield of protein expression in prokaryotic cells and purified protein preparations. This dataset...
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Biotechnological production and purification of Borrelia burgdorferi sl B2 protein
Dane BadawczeBorrelia burgdorferi B2 protein could be used in the detection of specific immunoglobulins from patients' sera with Lyme disease that may be infected with different genospecies of B. burgdorferii sl. The goal of the study was to obtain the highest possible yield of protein expression in prokaryotic cells and purified protein preparations. This dataset...
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Biotechnological production and purification of Borrelia burgdorferi sl B12 protein
Dane BadawczeBorrelia burgdorferi B12 protein could be used in the detection of specific immunoglobulins from patients' sera with Lyme disease that may be infected with different genospecies of B. burgdorferii sl. The goal of the study was to obtain the highest possible yield of protein expression in prokaryotic cells and purified protein preparations. This dataset...
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Biotechnological production and purification of Borrelia burgdorferi sl B3 protein
Dane BadawczeBorrelia burgdorferi B3 protein could be used in the detection of specific immunoglobulins from patients' sera with Lyme disease that may be infected with different genospecies of B. burgdorferii sl. The goal of the study was to obtain the highest possible yield of protein expression in prokaryotic cells and purified protein preparations. This dataset...
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Biotechnological production and purification of Borrelia burgdorferi sl B6 protein
Dane BadawczeBorrelia burgdorferi B6 protein could be used in the detection of specific immunoglobulins from patients' sera with Lyme disease that may be infected with different genospecies of B. burgdorferii sl. The goal of the study was to obtain the highest possible yield of protein expression in prokaryotic cells and purified protein preparations. This dataset...
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Recent phytochemical and pharmacological advances in the genus Potentilla L. sensu lato – An update covering the period from 2009 to 2020
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Biotechnological production and purification of Borrelia burgdorferi sl P protein
Dane BadawczeBorrelia burgdorferi P protein could be used in the detection of specific immunoglobulins G (IgG) from patients' sera with Lyme disease that may be infected with different genospecies of B. burgdorferii sl. The goal of the study was to obtain the highest possible yield of protein expression in prokaryotic cells and purified protein preparations. This...
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Biotechnological production and purification of Borrelia burgdorferi sl F protein
Dane BadawczeBorrelia burgdorferi F protein could be used in the detection of specific immunoglobulins G (IgG) from patients' sera with Lyme disease that may be infected with different genospecies of B. burgdorferii sl. The goal of the study was to obtain the highest possible yield of protein expression in prokaryotic cells and purified protein preparations. This...
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Nowe testy diagnostyczne oparte o antygeny rekombinantowe Toxoplasma gondii do wykrywania oraz różnicowania fazy wczesnej i przewlekłej toksoplazmozy
PublikacjaToksoplazmoza jest jedną z najbardziej powszechnych chorób pasożytniczych człowieka i zwierząt stałocieplnych. Na świecie jedna trzecia ludzkiej populacji jest chronicznie zarażona Toxoplasma gondii. Mimo, iż choroba ta u zdrowych,dorosłych osób zazwyczaj ma charakter bezobjawowy, bardzo poważne objawy mogą wystąpić w przypadku wrodzonego zarażenia oraz u osób z niedoborem odporności. Ponadto, pomimo dokładnego poznania jej etiologii...
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Lucyna Holec-Gąsior dr hab. inż.
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Antibody Cross-Reactivity in Serodiagnosis of Lyme Disease
PublikacjaLyme disease is a tick-borne disease caused by spirochetes belonging to the Borrelia burgdorferi sensu lato complex. The disease is characterized by a varied course; therefore, the basis for diagnosis is laboratory methods. Currently, a two-tiered serological test is recommended, using an ELISA as a screening test and a Western blot as a confirmatory test. This approach was introduced due to the relatively high number of false-positive...
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Reactivity of recombinant B12 protein with specific anti-B. burgdorferi s.l. antibodies present in human sera
Dane BadawczeBiotechnologically produced B12 antigens from three genospecies of Borrelia burgdorferi s.l. were assessed for their reactivity with specific anti-B. burgdorferi s.l. antibodies by Western blot to determine their utility for serological diagnosis of Borrelia infection in human sera.
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Reactivity of recombinant B6 protein with specific anti-B. burgdorferi s.l. antibodies present in human sera
Dane BadawczeBiotechnologically produced B6 antigens from three genospecies of Borrelia burgdorferi s.l. were assessed for their reactivity with specific anti-B. burgdorferi s.l. antibodies by Western blot to determine their utility for serological diagnosis of Borrelia infection in human sera.
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Reactivity of recombinant B2 protein with specific anti-B. burgdorferi s.l. antibodies present in human sera
Dane BadawczeBiotechnologically produced B2 antigens from three genospecies of Borrelia burgdorferi s.l. were assessed for their reactivity with specific anti-B. burgdorferi s.l. antibodies by Western blot to determine their utility for serological diagnosis of Borrelia infection in human sera.
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Reactivity of recombinant B1 protein with specific anti-B. burgdorferi s.l. antibodies present in human sera
Dane BadawczeBiotechnologically produced B1 antigens from three genospecies of Borrelia burgdorferi s.l. were assessed for their reactivity with specific anti-B. burgdorferi s.l. antibodies by IgG/IgM Western blot and IgG/IgM ELISA to determine their utility for serological diagnosis of Borrelia infection in human sera.
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Reactivity of recombinant B3 protein with specific anti-B. burgdorferi s.l. antibodies present in human sera
Dane BadawczeBiotechnologically produced B3 antigens from three genospecies of Borrelia burgdorferi s.l. were assessed for their reactivity with specific anti-B. burgdorferi s.l. antibodies by IgG/IgM Western blot and IgG/IgM ELISA to determine their utility for serological diagnosis of Borrelia infection in human sera.
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Toxoplasma gondii: Chimeric Dr fimbriae as a recombinant vaccine against toxoplasmosis
PublikacjaW publikacji przedstawiono protekcyjne i immunogenne właściowści chimerycznych fimbrii typu Dr zawierających sekwencje epitopowe antygenów SAG1, MAG1 oraz GRA1 Toxoplasma gondii. Uzyskane wyniki porównano z właściwościami immunogennymi antygenowych białek rekombinantowych oraz poliwalentnego antygenu natywnego. Antygeny rekombinantowe indukują silną odpowiedź immunologiczną i w 89% zapobiegają formowaniu się cyst w mózgu myszy....
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Construction of recombinant plasmid coding Borrelia burgdorferii sl F protein
Dane BadawczeDiagnosis of Lyme borreliosis (LB), an infection caused by spirochaetes of the B. burgdorferi sl species complex, is mainly based on clinical symptoms supported with serology and is often misdiagnosed in areas of endemicity. The complexity of the antigenic composition among the Borrelia genospecies and differential expression of proteins in host and...
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Nowe chimeryczne antygeny rekombinantowe Toxoplasma gondii - badanie aktywności immunogennej i ochronnej
ProjektyProjekt realizowany w Wydział Chemiczny zgodnie z porozumieniem UMO-2015/17/B/NZ6/03480 z dnia 2016-04-20
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GRA2 and ROP1 recombinant antigens as potential markers for detection of Toxoplasma gondii-specific immunoglobulin G in humans with acute toxoplasmosis.
PublikacjaArtykuł przedstawia oszacowanie przydatności w serodiagnostyce toksoplazmozy dwóch rekombinatowych białek antygenowych GRA2 i ROP1 Toxoplasma gondii. Białka rekombinantowe otrzymano z wykorzystaniem bardzo wydajnego, bakteryjnego systemu ekspresyjnego, który pozwolił na otrzymanie miligramowych ilości antygenów z 1 litra hodowli po zastosowaniu jednoetapowego oczyszczania metodą chromatografii metalopowinowactwa. Użyteczność diagnostyczna...
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Bartłomiej Ferra dr inż.
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