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Wyniki wyszukiwania dla: ls-dyna
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Analysis of induction of DNA DSBs on telomeres in A549 cells
Dane BadawczeData consist of an analysis of DNA DSBs on telomeres. A549 were treated with compounds (TXT4, MTX, DMSO) at IC90 concentration for the indicated time. Images were acquired with an LSM 800 inverted laser scanning confocal microscope (Carl Zeiss; Dresden, Germany) equipped with an Airyscan detector using a ×63 1.4 NA Plan Apochromat objective (Carl Zeiss)....
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Phylogenetic trees of genus Oncidium Sw. based on analysis of DNA sequences
Dane BadawczeGenus Oncidium Sw. is widely regarded as a polyphiletic, and the taxonomic boundaries between him and such genera as Odontoglossum Kunth. or Miltonia Lindley remain blurred. The goal of the study was to determine the phylogenetic relationships within the genus Oncidium s.lato based on the DNA sequences analysis. The correlation between molecular data...
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Molecular differences in mitochondrial DNA (mtDNA) genomes of dogs with malignant mammary tumours
Dane BadawczeThe aim of this study was to determine molecular defects in mitochondrial DNA with the use of large-scale genome analysis in malignant canine mammary gland tumours and indicate whether these changes were linked with the carcinogenesis process. With the use of the NGS technology, we sequenced 27 samples of mtDNA isolated from blood and tumours obtained...
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Confocal microscopy analysis of DNA DSB in telomerase negative cells after exposure to TXT2 and TXT4
Dane BadawczeThe data sets contain confocal microscopic images showing the γ-H2AX with TRF2 after treatment of NHBE and U2OS cell lines with TXT2 and TXT4 in equitoxic concentrations. Images were acquired with an LSM 800 inverted laser scanning confocal microscope (Carl Zeiss; Dresden, Germany) equipped with an Airyscan detector using a ×63 1.4 NA Plan Apochromat...
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Flow cytometry analysis of DNA DSBs in telomerase positive and negative cell lines after treatment with TXT2 and TXT4
Dane BadawczeFlow cytometry analysis of DNA DSBs in telomerase positive (A549, H460) and negative (NHBE2594, U2OS) cell lines after treatment with equitoxic concentrations of TXT2 and TXT4. MTX and DMSO were used as positive and negative controls, respectively.
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Study of the ability of TXT-compounds to promote telomeric G4 DNA thermal stabilization using FRET melting assay
Dane BadawczeThe data set contains a DNA melting screen obtained from the FRET melting assay. Thermal stabilities of G-quadruplex structures were analysed in the presence and in absence of ligands. TMPyP4 and MTX were used as references. Measurements were carried out in triplicate using a LightCycler 480 thermocycler (Roche Life Science) with a filter setting of...
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ArchBGal32cB 441Glu mutein gene analysis dataset
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Effect of new bisacridines IKE16, IKE18 and IE10 on the yeast topoisomerase II relaxation activity
Dane BadawczeThe datasets contain the results of new bisacridines IKE16, IKE18 and IE10 inhibition activity against yeast topoisomerase II. DNA topoisomerases (Topo) are enzymes that catalyze changes in the spatial structure of DNA and play an important role in replication, transcription and recombination. Beyond their normal functions, DNA topo are significant...
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Simulations of wave propagation and attenuation in fields of colliding ice floes
Dane BadawczeThis dataset contains results of numerical smulations of sea ice-wave interactions, corresponding to laboratory experiments conducted at the Large Ice Model Basin (LIMB) at the Hamburg Ship Model Basin (HSVA) as part of the LS-WICE ("Loads on Structure and Waves in Ice"; https://zenodo.org/record/1067170#.XrLt_dhpxhE) project. THe simulations were conducted...
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D-loop sequences retrieved from Canis lupus familiaris mitochondrial genome
Dane BadawczeCanine mitochondrial genome is built of 16727 bp. Non-coding control region (mtCR), called also D-loop, begins with 15458 nucleotide and ends with 16727 nucleotide. The length of this fragment is 1270 bp (Kim et al., 1998). D-loop region is responsible for replication and transcription of mitochondrial DNA. Mutations that occur within it may cause irregularity...
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Sequences of internal transcribed spacer 1, 5.8S ribosomal RNA gene and internal transcribed spacer 2 of psychrotolerant pigment-producing yeast-like fungi
Dane BadawczeDNA encoding ITS1-5.8S-ITS2 fragments of psychrotolerant pigment-producing yeast-like fungi named Red, Pink and Black were PCR amplified using ITS1 5' TCCGTAGGTGAACCTGCGG 3' and ITS4 5’ TCCTCCGCTTATTGATATGC 3’ primers and sequenced by Sanger method using the same primers.
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Sequences of large subunit ribosomal RNA gene, partial sequence encoding D1/D2 domain of psychrotolerant pigment-producing yeast-like fungi
Dane BadawczeDNA fragments encoding D1/D2 domain of large subunit ribosomal RNA of psychrotolerant pigment-producing yeast-like fungi named Red, Pink and Black were PCR amplified using NL1 5’ GCATATCAATAAGCGGAGGAAAAG 3’ and NL4 5’ GGTCCGTGTTTCAAGACGG 3’ primers and sequenced by Sanger method using the same primers.
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TRNA-Leu (UUR) gene haplotypes observed in canine mammary gland tumours and its deleterious effect assessment according to the comparative analysis with TRNL1 human gene
Dane BadawczeThe aetiology and pathogenesis of many canine tumours are likely to be similar to cancers found in humans. This study aimed to present a plausible link between changes in tRNA-Leu (UUR) gene and the carcinogenesis process in dogs with mammary gland tumours. The whole mitochondrial DNA (mtDNA) isolated from blood and tumour tissues of 13 dogs with malig-nant...
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Identification and cloning of C. albicans SC5314 genes encoding L-methionine biosynthetic pathway enzymes.
Dane BadawczeEnzymes of fungal L-methionine biosynthetic pathway: homoserine O-acetyltransferase (Met2p), O-acetylhomoserine sulfhydrylase (Met15p) and cystathionine-γ-synthase (Str2p) could be exploited as molecular targets for antifungal chemotherapy. The goal of the study was to identify and clone genes encoding mentioned above enzymes. MET2, MET15 and STR2 genes...
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Data on solutions of Hes1 system
Dane BadawczeHes1 protein (hairy and enhancer of split 1) belongs to the helix-loop-helix (bHLH) family of transcription proteins, i.e. DNA-binding proteins in the promoter region or in another region where regulation of transcription processes occurs. The database collects data on solutions of the Hes1 systems with multiple binding sites and the dimer formation...
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IKE1-IKE3 (C-1305 derivatives) inhibitory effect of the Yeast Topoisomerase II relaxation activity
Dane BadawczeInhibition of Yeast Topoisomerase II were analyzed according to relaxation assay kit from Inspiralis. Briefly, 250 ng of supercoiled pBR322 DNA, 1 mM ATP, 1-200 μM of analyzed compound were mixed with reaction buffer (1 mM Tris-HCl (pH 7.9), 10 mM KCl, 0.5 mM MgCl 2, 0.2 % (v/v) glycerol). The reaction was initiated by the addition of an enzyme, allowed...
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Topographic AFM imaging of the leaf surface with magnification of details of its morphological structure
Dane BadawczeTopographic imaging of the leaf surface with magnification of details of its morphological structure. Measurements in semi-contact mode. NTEGRA Prima (NT-MDT) device. NSG 01 probe.
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Dataset of non-isomorphic graphs of the coloring types (K4,K4;n), 1<n<R(4,4)
Dane BadawczeFor K4 graph, a coloring type (K4,K4;n) is such an edge coloring of the full Kn graph, which does not have the K4 subgraph in the first color (representing by no edges in the graph) or the K4 subgraph in the second color (representing by edges in the graph).The Ramsey number R(4,4) is the smallest natural number n such that for any edge coloring of...
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Dataset of non-isomorphic graphs being coloring types (K5-e,Km-e;n), 2<m<5, 1<n<R(K5-e,Km-e)
Dane BadawczeFor K5-e and Km-e graphs, the type coloring (K5-e,Km-e;n) is such an edge coloring of the full Kn graph, which does not have the K5-e subgraph in the first color (no edge in the graph) or the Km-e subgraph in the second color (exists edge in the graph). Km-e means the full Km graph with one edge removed.The Ramsey number R(K5-e,Km-e) is the smallest...
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Dataset of non-isomorphic graphs being coloring types (K6-e,Km-e;n), 2<m<5, 1<n<R(K6-e,Km-e)
Dane BadawczeFor K6-e and Km-e graphs, the type coloring (K6-e,Km-e;n) is such an edge coloring of the full Kn graph, which does not have the K6-e subgraph in the first color (no edge in the graph) or the Km-e subgraph in the second color (exists edge in the graph). Km-e means the full Km graph with one edge removed. The Ramsey number R(K6-e,Km-e) is the smallest...