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Search results for: hplc-dad
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Simultaneous detrmination of selected phenoxyacid herbicides and chlorophenols in surface and seawater by HPLC coupled to DAD.
PublicationDo badań wykorzystano metodę ekstrakcji do fazy stałej (SPE) w celu wzbogacenia analitów. Ekstrakty analizowano techniką wysokosprawnej chromatografii cieczowej (HPLC) z detekcją DAD. Opracowana metoda umożliwia oznaczenie 14 związków z grupy kwasów fenoksyoctowych i chlorofenoli w czasie 22 min. Odzysk analitów wynosił powyżej 60% dla fenoksykwasów i powyżej 70% w przypadku chlorofenoli. Granica oznaczalności dla oznaczania...
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In Vitro Anticariogenic Effects of Drymocallis rupestris Extracts and Their Quality Evaluation by HPLC-DAD-MS3 Analysis
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In vitro anticariogenic effects of Rubus caesius extracts and their quality evaluation by HPLC-DAD-MS3 analysis
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Phenolic composition and antioxidant properties of polish blue-berried honeysuckle genotypes by HPLC-DAD-MS, HPLC postcolumn derivatization with ABTS or FC, and TLC with DPPH visualization
PublicationIn this study, different Polish cultivars of blue-berried honeysuckles (Lonicera caerulea L.), wild and bog bilberry, were analyzed for bioactive compounds. The chemical properties verified included composition of anthocyanins and other polyphenols, antioxidant activity, and profiles of antioxidants by HPLC postcolumn derivatization or TLC. The antioxidant activities of different blue-berried honeysuckle cultivars were similar...
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The profiles and spectrum of aqua solution of tannic acid by HPLC-DAD-MS analysis.
Open Research DataThe profiling of tannic acid is determined by examining the content and proportion / ratio of gallotannins [Aras et al. 2016, Clifford et al. 2007, Gauri et al. 2012, Nishizawa et al. 1982]. In order to check the ion mass distribution (TIC) in an aqueous solution of commercial tannic acid, a direct sample injection (TA) was performed into the mass spectrometer....
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HPLC–DAD Analysis, Antimicrobial and Antioxidant Properties of Aromatic Herb Melissa officinalis L., Aerial Parts Extracts
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HPLC-DAD in identification and quantification of selected coumarins in crude extracts from plant cultures of Ammi majus and Ruta graveolens.
PublicationW pracy opisano wyniki badań nad opracowaniem metody rozdzielania, identyfikacji i oznaczania wybranych kumaryn i funarokumaryn w surowych ekstraktach roślinnych z Ammi majus i Ruta graveolens hodowanych pojedynczo lub w kokulturach. Zbadano przydatność trzech głównych składników eluentu, stosowanych w układach faz odwróconych i różnych programów elucji: woda- acetonitryl(ACN)- metanol (MeOH)- tetrahydrofuran (THF). W optymalnej...
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Exploring DAD and ADD Methods for Dealing with Urban Heat Island Effect
PublicationThe Urban Heat Island (UHI) effect in the context of climate change and temperature fluctuations is an increasing challenge for contemporary cities. Numerous activities focus on mitigation and adaptation to the UHI effect using both appropriately selected design strategies and technological solutions. However, not all of these technologies support the postulates of ecological and low-carbon cities. Their design, implementation,...
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Simultaneous determination of individual isothiocyanates in plant samples by HPLC-DAD-MS following SPE and derivatization with N-acetyl-l-cysteine
PublicationThe procedure for the isothiocyanates (ITCs) determination that involves derivatization with N-acetyl-l-cysteine (NAC) and separation by HPLC was developed. Prior to derivatization, plant ITCs were isolated and purified using solid-phase extraction (SPE). The optimum conditions of derivatization are: 500 μL of isopropanolic eluate obtained by SPE combined with 500 μL of derivatizing reagent (0.2 M NAC and 0.2 M NaHCO3 in water)...
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Metodyka oznaczania sumarycznej zawartości inhibitorów fermentacji ciemnej oraz monocukrów w brzeczkach fermentacyjnych techniką HPLC-RID-UV-VIS/DAD
PublicationW pracy przedstawiono wyniki badań oraz opracowaną w ich rezultacie metodykę ilościowego oznaczania sumarycznej zawartości inhibitorów fermentacji ciemnej oraz monocukrów w brzeczkach fermentacyjnych z wykorzystaniem techniki chromatografii cieczowej (RP-HPLC-RID-UV-VIS/DAD). Najbardziej przydatną do zbadania sumarycznej zawartości inhibitorów w próbkach rzeczywistych okazało się zastosowanie warunków chromatografii wykluczania...