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Search results for: recombinant strains
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Study of the influence of the presence of Dr fimbriae on the adherence of cells of the recombinant Escherichia coli strain: BL21(DE3)/pACYCpBAD to polystyrene in a dynamic system
Open Research DataThe attachment of bacteria begins the process of surface colonization, called biofilm development, characterized by a number of physicochemical and molecular interactions. Adherence to inert surfaces typically involves nonspecific interactions, whereas adherence to biological surfaces is associated with specific ligand-receptor interactions. In this...
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Isolation and bioinformatic analysis of seven genes encoding potato apyrase. Bacterial overexpresssion, refolding and initial kinetic studies on some recombinant potato apyrases
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Toxoplasma gondii: Enzyme-Linked Immunosorbent Assay using different fragments of recombinant microneme protein 1 (MIC1) for detection of immunoglobulin G antibodies
PublicationTrzy rekombinantowe fragmenty białka antygenowego MIC1 Toxoplasma gondii (r-MIC1ex2, r-MIC1ex34 i r-MIC1) zostały wyprodukowane jako białka fuzyjne (zawierające dwie domeny oligohistydynowe na N- i C-końcu) w komórkach bakteryjnych Escherichia coli. Homogenne preparaty antygenów rekombinantowych otrzymane z zastosowaniem jednoetapowego oczyszczania metodą chromatografii metalopowinowactwa, wykorzystano następnie do immunoidentyfikacji...
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Prime-Boost Vaccination with Toxoplasma Lysate Antigen, but Not with a Mixture of Recombinant Protein Antigens, Leads to Reduction of Brain Cyst Formation in BALB/c Mice
PublicationIntroduction Infection with the ubiquitous parasite Toxoplasma gondii is a threat for immunocompro- mised patients and pregnant women and effective immune-prophylaxis is still lacking. Methods Here we tested a mixture of recombinant T . gondii antigens expressed in different develop- mental stages, i.e., SAG1, MAG1 and GRA7 (SMG), and a lysate derived from T . gondii tachyzoites (TLA) for prophylactic vaccination against cyst formation....
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Investigation of the reliability of local strain analysis by means of the triangle modelling
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The pressure–strain work indices in response to isometric handgrip exercise
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Weak Solutions within the Gradient-Incomplete Strain-Gradient Elasticity
PublicationIn this paper we consider existence and uniqueness of the three-dimensional static boundary-value problems in the framework of so-called gradient-incomplete strain-gradient elasticity. We call the strain-gradient elasticity model gradient-incomplete such model where the considered strain energy density depends on displacements and only on some specific partial derivatives of displacements of first- and second-order. Such models...
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Refinement of the Hardening Soil model within the small strain range
PublicationThe popularity of the elasto-plastic Hardening Soil (HS) model is based on simple parameter identification from standard testing and empirical formulas. The HS model is implemented in many commercial FE codes designed to analyse geotechnical problems. In its basic version, the stress–strain behaviour within the elastic range is subject to the hypoelastic power law, which assures the barotropy of the elastic stiffness. However,...
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Ewaluation of a PCR Melting Profile technique for bacterial strain differentiation
PublicationBadano przydatność techniki PCR MP w efektywnym typowaniu szczepów w badaniach epidemiologicznych zakażeń szpitalnych. Technika oparta jest na użyciu niskiej temperatury denaturacji podczas LM PCR. Badaniu zostały poddane izolaty Escherichia coli pochodzące od pacjentów Szpitala Klinicznego w Gdańsku. Wykazano, że technika jest szybka, o wysokim potencjale różnicującym i bardzo dobrej powtarzalności i może być zastosowana w badaniach...
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Strain field of abdominal surface due to patients live excitations
PublicationPodano metodę opisu pola odkształceń powierzchni brzucha pacjenta podczas ruchów jego tułowia. Wyróżniono strefy brzucha o zróżnicowanej wielkości odkształceń ekstremalnych.
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Time-of-flight fiber optic sensors for strain and temperature measurement
PublicationW pracy przedstawiono światłowodowy sensor czasu przelotu do pomiaru odkształceń i temperatury mogący pracować zarówno w modzie transmisyjnym, jak i odbiciowym. W pomiarach czasu przelotu wykorzystano krótkie impulsy światłowodowe wprowadzane do jednodomowego światłowodu. Pod wpływem czynników zewnętrznych (temperatura, odkształcenie, siła rozciągająca) zmieniała się droga optyczna w światłowodzie, co powodowało zmiany czasu przelotu...
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Stress strain relations and modulus of elascity of two stage concrete.
PublicationOmówiono badania doświadczalne przeprowadzone dla betonu dwuetapowego. Beton wykonano z trzech różnych kruszyw przy zastosowaniu trzech różnych proporcji w/c i c/p. Wyznaczono związki pomiędzy naprężeniami i odkształceniami. Na drodze statystycznej opracowano wzory umożliwiające wstępne szacowanie wartości siecznego modułu Younga. Ponieważ metoda dwuetapowa pozwala na zachowanie kontaktu pomiędzy poszczególnymi elementami kamiennego...
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Scale effects in granular bodies during plane strain compression.
PublicationW arykule omówiono efekty skali w materiałach granulowanych spowodowanych wielkością próbek. Obliczenia wykonano stosując MES na bazie prawa hipoplastycznego rozszerzonego o długość charakterystyczną. Porównano różne metody regularyzacji.
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Trans Theorizing
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Trans Theorizing
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Antimicrobial Resistance and Virulence Characterization of Listeria monocytogenes Strains Isolated from Food and Food Processing Environments
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Biofilm Formation and Prevalence of Biofilm-Related Genes Among Clinical Strains of Multidrug-Resistant Staphylococcus aureus
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Rotating Magnetic Field Increases β-Lactam Antibiotic Susceptibility of Methicillin-Resistant Staphylococcus aureus Strains
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A comparative pathogenicity analysis of two adenovirus strains, 1/A and 8a/E, isolated from poultry in Poland
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Genotyping and characterization of virulence factors in Escherichia coli strains isolated from patients from Polish hospital
PublicationZakażenia układu moczowego (UTIs - ang. urinary track infections) są jedną z najczęstszy infekcji, za które w 85% przypadków odpowiedzialne są bakterie Escherichia coli. Infekcje układu moczowego mogą prowadzić do groźnych powikłań m. in. odmiedniczkowego zapalenia nerek. Śmiertelność wśród tej grupy pacjentów wynosi 1-3%. Zakażenia układu moczowego występują również u ok. 7% kobiet ciężarnych. W pracy przebadano 82 szczepy E....
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Increased Levels of (p)ppGpp Correlate with Virulence and Biofilm Formation, but Not with Growth, in Strains of Uropathogenic Escherichia coli
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Molecular characterization of drug-resistant Mycobacterium tuberculosis strains from Poland by use of multiple genetic markers
PublicationW pracy przedstawiono wyniki genotypowania wielolekoopornych szczepów Mycobacterium tuberculosis, izolowanych od pacjentów z Polski.
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Self-organising maps in the analysis of strains of human abdominal wall to identify areas of similar mechanical behaviour.
PublicationThe study refers to the application of a type of artificial neural network called the Self-Organising Map (SOM) for the identification of areas of the human abdominal wall that behave in a similar mechanical way. The research is based on data acquired during in vivo tests using the digital image correlation technique (DIC). The mechanical behaviour of the human abdominal wall is analysed during changing intra-abdominal pressure....
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Condition Monitoring of Metallized Polypropylene Film Capacitors in Railway Power Trains
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A method for predicting main propulsion power for inland waterways push trains
PublicationPraca zawiera założenia teoretyczne i model matematyczny metody służącej do wyznaczania wstępnej, przybliżonej prognozy wielkości zapotrzebowanej mocy napędu głównego śródlądowego zestawu pchanego.
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Influence of Antimony Substitution on Spontaneous Strain and Thermodynamic Stability of Lanthanum Orthoniobate
PublicationThe analysis of the antimony substitution influence on temperature dependence of unit cell distortion has been done. The values of spontaneous strain and Landau order parameter for three different antimony contents varied had been calculated. The phase transition occurring for antimony substituted lanthanum orthoniobate was found to be second order. The high temperature solution calorimetry method has been used to investigate...
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Direct shear stress vs strain relation for fiber reinforced composites
PublicationThe majority of fiber reinforced composites exhibit strong non-linear behavior in in-plane shear state. The effect is attributed to the micro-cracks appearing in the matrix and can be modeled on the micro and macro level. In this work the author proposes constitutive laws describing the non-linear in-plane shear response, which can be alternative for the relations commonly considered in the literature. The proposed equations are...
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Determination of equivalent axle load factors with the use of strain energy of distortion
PublicationThe paper proposes a new method for calculation of equivalent axle load factors based on the analysis of strain energy of distortion induced in road pavements by traffic loads. The main advantage of the method is the more accurate calculation of the effects of multiple axles and super single versus dual tyres. The methodconsiders the location of critical points, at which strain energy of distortion reaches extreme values. When...
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Surface effects of network materials based on strain gradient homogenized media
PublicationThe asymptotic homogenization of periodic network materials modeled as beam networks is pursued in this contribution, accounting for surface effects arising from the presence of a thin coating on the surface of the structural beam elements of the network. Cauchy and second gradient effective continua are considered and enhanced by the consideration of surface effects. The asymptotic homogenization technique is here extended to...
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FE-modelling of strain localization in concrete with coupled elasto-plasticity and damage
PublicationW artykule przedstawiono wyniki numerycznej dla zginania belki betonowej przy zastosowaniu modelu sprężysto-plastycznego, modelu z degradacja sztywności i modelu połączonego sprężysto-plastycznego z degradacją sztywności. Wszystkie modele rozszerzono o długość charakterystyczną mikrostruktury za pomocą teorii nielokalnej.
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Investigations of size effects in granular bodies during plane strain compression
PublicationArtykuł omawia deterministyczny i statystyczny efekt skali w materiałach granulowanych podczas ściskania w płaskim stanie odkształcenia. Obliczenia wykonano metodą elementów skończonych na bazie mikropolarnego prawa hipoplastycznego. Wykorzystano metodę hiperłacińskiego próbkowania w celu redukcji ilości realizacji.
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Boundary effects on behaviour of granular material during plane strain compression
PublicationW artykule przedstawiono wyniki symulacji lokalizacji odkształceń w materiałach granulowanych podzcas sciskania dwuosiowego. Obliczenia wykonano przy zastosowaniu metody elementów skończonych i mikropolarnego modelu hipoplastycznego. W pracy pokazano wpływ warunków brzegowych na globalne i loklane zachowanie sie materiału.
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Effect of heterogeneity on shear zone formation during plane strain compression.
PublicationW artykule przedstawiono wyniki numerycznej analizy ściskania w płaskim stanie odkształcenia dla materiałów granulowanych. Obliczenia przeprowdzono z modelem hipoplastycznym rozszerzonym o obroty i długość charakterystyczną. Obliczenia wykonno dla różnych rodzajów imperfekcji w próbce piasku zagęszczonego i luźnego.
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Anti-Helicobacter pylori activities of selected N-substituted cinnamamide derivatives evaluated on reference and clinical bacterial strains
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Joint Genomic and Proteomic Analysis Identifies Meta-Trait Characteristics of Virulent and Non-virulent Staphylococcus aureus Strains
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Physico‐chemical and proteolytic changes during cold storage of dry‐cured pork loins with probiotic strains ofLAB
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Stability of Antiradical Activity of Protein Extracts and Hydrolysates from Dry-Cured Pork Loins with Probiotic Strains of LAB
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Deep analysis of Loop L1 HVRs1-4 region of the hexon gene of adenovirus field strains isolated in Poland
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Characterisation of adenovirus strains represented species B and E isolated from broiler chicken flocks in eastern Poland
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Linezolid-resistant Enterococcus faecium strains isolated from one hospital in Poland –commensals or hospital-adapted pathogens?
PublicationOne of the most pressing problems of enterococci infections is occurring resistance to linezolid, which is an antibiotic used in the treatment of infections caused by vancomycin-resistant strains (VRE). The main objective of our research was to investigate the relationship of 19 linezolid-resistant E. faecium isolates from 18 patients hospitalized at Clinical Hospital in Gdansk (Poland). One of the LZDREF was isolated in 2003...
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Full-field in vivo experimental study of the strains of a breathing human abdominal wall with intra-abdominal pressure variation
PublicationThe presented study aims to assess the mechanical behaviour of the anterior abdominal wall based on an in vivo experiment on humans. Full-field measurement of abdominal wall displacement during changes of intra-abdominal pressure is performed using a digital image correlation (DIC) system. Continuous measurement in time enables the observation of changes in the strain field during breathing. The understanding of the mechanical...
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How the Depths of the Danish Straits Shape Gdańsk's Port and City Spatial Development
PublicationThe depths of the Danish Straits limit the drafts of ships entering the Baltic Sea. The largest ships calling the Baltic in a laden condition are called Baltimax. The article presents how the dredging works carried out in the Danish Straits in the 1970s enabled the development of the Port of Gdańsk and consequently also influenced the city, being a residential base for employees of the new port and shipyards. The analysed case...
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New possible pharmacological targets for statins and ezetimibe
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COVID-19: Direct and Indirect Mechanisms of Statins
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Strain gauge results
Open Research DataThe aim of the research project is to determine the rotational stiffness of the connection between the purlin and the part of the truss top chord. The attached files are referred to strain gauge results placed on 16 specimens. For the specimens number from 1 to 8 the magnitudes should be multiplied by factor -2 and for the others by factor -1.
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Train the trainer course
PublicationThis chapter presents the concept, evaluation and evaluation results for the train the trainer. This concept of train the trainers is prepared within Workpackage 5 of EU-funded project: MASTER BSR (Erasmus+ Strategic Partnership Programme). Due to the nature of adult learning the content is designed for the use of participatory methods (involved, active). This method uses various techniques of active learning e.g. group work,...
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Visual impairment and traits of autism in children
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Fast Approximate String Search for Wikification
PublicationThe paper presents a novel method for fast approximate string search based on neural distance metrics embeddings. Our research is focused primarily on applying the proposed method for entity retrieval in the Wikification process, which is similar to edit distance-based similarity search on the typical dictionary. The proposed method has been compared with symmetric delete spelling correction algorithm and proven to be more efficient...
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Transient torsional vibrations control in the geared drive trains of the hot rolling mills
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The thermodynamic critical field and specific heat of superconducting state in phosphorene under strain
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