Electrochemical and in silico approaches for liver metabolic oxidation of antitumor-active triazoloacridinone C -1305 - Publikacja - MOST Wiedzy

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Electrochemical and in silico approaches for liver metabolic oxidation of antitumor-active triazoloacridinone C -1305

Abstrakt

5-Dimethylaminopropylamino-8-hydroxytriazoloacridinone (C-1305) is a promising antitumor compound developed in our laboratory. A better understanding of its metabolic transformations is still needed to explain the multidirectional mechanism of pharmacological action of triazoloacridinone derivatives at all. Thus, the aim of the current work was to predict oxidative pathways of C-1305 that would reflect its phase I metabolism. The multi-tool analysis of C-1305 metabolism included electrochemical conversion and in silico sites of metabolism predictions in relation to liver microsomal model. In the framework of the first approach, an electrochemical cell was coupled on-line to an electrospray ionization mass spectrometer. The effluent of the electrochemical cell was also injected onto a liquid chromatography column for the separation of different products formed prior to mass spectrometry analysis. In silico studies were performed using MetaSite software. Standard microsomal incubation was employed as a reference procedure. We found that C-1305 underwent electrochemical oxidation primarily on the dialkylaminoalkylamino moiety. An unknown so far N-dealkylated and hydroxylated C-1305 products have been identified. The electrochemical system was also able to simulate oxygenation reactions. Similar pattern of C-1305 metabolism has been predicted using in silico approach. Both proposed strategies showed high agreement in relation to the generated metabolic products of C-1305. Thus, we concluded that they can be considered as simple alternatives to enzymatic assays, affording time and cost efficiency.

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Informacje szczegółowe

Kategoria:
Publikacja w czasopiśmie
Typ:
artykuły w czasopismach
Opublikowano w:
Journal of Pharmaceutical Analysis nr 10, strony 376 - 384,
ISSN: 2095-1779
Język:
angielski
Rok wydania:
2020
Opis bibliograficzny:
Potęga A., Żelaszczyk D., Mazerska Z.: Electrochemical and in silico approaches for liver metabolic oxidation of antitumor-active triazoloacridinone C -1305// Journal of Pharmaceutical Analysis -Vol. 10,iss. 4 (2020), s.376-384
DOI:
Cyfrowy identyfikator dokumentu elektronicznego (otwiera się w nowej karcie) 10.1016/j.jpha.2020.03.011
Weryfikacja:
Politechnika Gdańska

wyświetlono 21 razy

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