Modified DNA polymerases for PCR troubleshooting


PCR has become an essential tool in biological science. However, researchers often encounter problems with difficult targets, inhibitors accompanying the samples, or PCR trouble related to DNA polymerase. Therefore, PCR optimization is necessary to obtain better results. One solution is using modified DNA polymerases with desirable properties for the experiments. In this article, PCR troubleshooting, depending on the DNA polymerase used, is shown. In addition, the reasons that might justify the need for modification of DNA polymerases, type of modifications, and links between modified DNA polymerases and PCR efficiency are described.


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Informacje szczegółowe

Kategoria: Publikacja w czasopiśmie
Typ: artykuł w czasopiśmie wyróżnionym w JCR
Opublikowano w: JOURNAL OF APPLIED GENETICS nr 58, strony 133 - 142,
ISSN: 1234-1983
Język: angielski
Rok wydania: 2017
Opis bibliograficzny: Śpibida M., Krawczyk B., Olszewski M., Kur J.: Modified DNA polymerases for PCR troubleshooting// JOURNAL OF APPLIED GENETICS. -Vol. 58, nr. 1 (2017), s.133-142
DOI: 10.1007/s13353-016-0371-4
wyświetlono 15 razy
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