Wyszukiwarka
Abstrakt
The current surge in bacterial multi-drug resistance (MDR) is one of the largest challenges to public health, threatening to render ineffective many therapies we rely on for treatment of serious infections. Understanding different factors that contribute to MDR is hence crucial from the global “one health” perspective. In this contribution, we focus on the prototypical broad-selectivity proton-coupled antiporter EmrE, one of the smallest known ligand transporters that confers resistance to aromatic cations in a number of clinically relevant species. As an asymmetric homodimer undergoing an “alternating access” protomer-swap conformational change, it serves as a model for the mechanistic understanding of more complex drug transporters. Here, we present a free energy and solvent accessibility analysis that indicates the presence of two complementary ligand translocation pathways that remain operative in a broad range of conditions. Our simulations show a previously undescribed desolvated apo state and anticorrelated accessibility in the ligand-bound state, explaining on a structural level why EmrE does not disrupt the pH gradient through futile proton transfer. By comparing the behavior of a number of model charged and/or aromatic ligands, we also explain the origin of selectivity of EmrE towards a broad class of aromatic cations. Finally, we explore unbiased pathways of ligand entry and exit to identify correlated structural changes implicated in ligand binding and release, as well as characterize key intermediates of occupancy changes.
Cytowania
-
9
CrossRef
-
0
Web of Science
-
6
Scopus
Autorzy (4)
Cytuj jako
Pełna treść
- Wersja publikacji
- Accepted albo Published Version
- DOI:
- Cyfrowy identyfikator dokumentu elektronicznego (otwiera się w nowej karcie) 10.1371/journal.pcbi.1009454
- Licencja
-
otwiera się w nowej karcie
Słowa kluczowe
Informacje szczegółowe
- Kategoria:
- Publikacja w czasopiśmie
- Typ:
- artykuły w czasopismach
- Opublikowano w:
-
PLoS Computational Biology
nr 17,
ISSN: 1553-7358 - Język:
- angielski
- Rok wydania:
- 2021
- Opis bibliograficzny:
- Jurasz J., Bagiński M., Czub J., Wieczór M.: Molecular mechanism of proton-coupled ligand translocation by the bacterial efflux pump EmrE// PLoS Computational Biology -Vol. 17,iss. 10 (2021), s.e1009454-
- DOI:
- Cyfrowy identyfikator dokumentu elektronicznego (otwiera się w nowej karcie) 10.1371/journal.pcbi.1009454
- Weryfikacja:
- Politechnika Gdańska
wyświetlono 82 razy
Publikacje, które mogą cię zainteresować
Imidazoacridinone-dependent lysosomal photodestruction: a pharmacological Trojan horse approach to eradicate multidrug-resistant cancers
- Y. Adar,
- M. Stark,
- E. E. Bram
- + 7 autorów