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Silver nanoparticles induce premutagenic DNA oxidation that can be prevented by phytochemicals from Gentiana asclepiadea
Abstrakt
Among nanomaterials, silver nanoparticles (AgNPs) have the broadest and most commercial applications due to their antibacterial properties, highlighting the need for exploring their potential toxicity and underlying mechanisms of action. Our main aim was to investigate whether AgNPs exert toxicity by inducing oxidative damage to DNA in human kidney HEK 293 cells. In addition, we tested whether this damage could be counteracted by plant extracts containing phytochemicals such as swertiamarin, mangiferin and homoorientin with high antioxidant abilities. We show that AgNPs (20nm) are taken up by cells and localised in vacuoles and cytoplasm. Exposure to 1, 25 or 100 µg/ml AgNPs leads to a significant dose-dependent increase in oxidised DNA base lesions (8-oxo-7,8-dihydroguanine or 8-oxoG) detected by the comet assay after incubation of nucleoids with 8-oxoG DNA glycosylase. Oxidised DNA base lesions and strand breaks caused by AgNPs were diminished by aqueous and methanolic extracts from both haulm and flower of Gentiana asclepiadea.
Cytowania
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3 9
CrossRef
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0
Web of Science
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4 3
Scopus
Autorzy (19)
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Wiggo Johan Sandberg
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Pełna treść
- Wersja publikacji
- Accepted albo Published Version
- Licencja
- Copyright (The Author 2012. Published by Oxford University Press)
Słowa kluczowe
Informacje szczegółowe
- Kategoria:
- Publikacja w czasopiśmie
- Typ:
- artykuł w czasopiśmie wyróżnionym w JCR
- Opublikowano w:
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MUTAGENESIS
nr 27,
wydanie 6,
strony 759 - 769,
ISSN: 0267-8357 - Język:
- angielski
- Rok wydania:
- 2012
- Opis bibliograficzny:
- Hudecová A., Kusznierewicz B., Rundén-Pran E., Magdolenová Z., Haąplová K., Rinna A., Fjellsbø L., Kruszewski M., Lankoff A., Sandberg W., Refsnes M., Skuland T., Schwarze P., Brunborg G., Bjøras M., Collins A., Miadoková E., Gálová E., Duąinská M.: Silver nanoparticles induce premutagenic DNA oxidation that can be prevented by phytochemicals from Gentiana asclepiadea// MUTAGENESIS. -Vol. 27, iss. 6 (2012), s.759-769
- DOI:
- Cyfrowy identyfikator dokumentu elektronicznego (otwiera się w nowej karcie) 10.1093/mutage/ges046
- Weryfikacja:
- Politechnika Gdańska
wyświetlono 163 razy
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