Wyszukiwarka
Gentiana asclepiadea exerts antioxidant activity and enhances DNA repair of hydrogen peroxide- and silver nanoparticles-induced DNA damage
Abstrakt
Exposure to high levels of different environmental pollutants is known to be associated with induction of DNA damage in humans. Thus DNA repair is of great importance in preventing mutations and contributes crucially to the prevention of cancer. In our study we have focused on quantitative analysis of Gentiana asclepiadea aqueous or methanolic extracts obtained from flower and haulm, their antioxidant potency in ABTS post-column derivatisation, and their potential ability to enhance DNA repair in human lymphocytesafter hydrogen peroxide (H2O2) treatment (250 lM, 5 min). We also studied DNA repair in human kidney HEK 293 cells after exposure to 20 nm silver nanoparticles (AgNPs) (100 lg/ml, 30 min) in the presence and absence of the plant extract. We have found that mangiferin along with unidentified polar compounds are the most pronounced antioxidants in the studied extracts. Extract from haulm exhibited slightly stronger antioxidant properties compared to flower extracts. However, all four extracts showed significant ability to enhance DNA repair in both cell types after H2O2 and AgNP treatments.
Cytowania
-
2 6
CrossRef
-
0
Web of Science
-
3 2
Scopus
Autorzy (8)
Cytuj jako
Pełna treść
- Wersja publikacji
- Accepted albo Published Version
- Licencja
-
otwiera się w nowej karcie
Słowa kluczowe
Informacje szczegółowe
- Kategoria:
- Publikacja w czasopiśmie
- Typ:
- artykuł w czasopiśmie wyróżnionym w JCR
- Opublikowano w:
-
FOOD AND CHEMICAL TOXICOLOGY
nr 50,
strony 3352 - 3359,
ISSN: 0278-6915 - Rok wydania:
- 2012
- Opis bibliograficzny:
- Hudecova A., Kusznierewicz B., Hasplova K., Huk A., Magdolenova Z., Miadokova E., Galova E., Dusinska M.: Gentiana asclepiadea exerts antioxidant activity and enhances DNA repair of hydrogen peroxide- and silver nanoparticles-induced DNA damage// FOOD AND CHEMICAL TOXICOLOGY. -Vol. 50, nr. iss. 9 (2012), s.3352-3359
- DOI:
- Cyfrowy identyfikator dokumentu elektronicznego (otwiera się w nowej karcie) 10.1016/j.fct.2012.06.017
- Weryfikacja:
- Politechnika Gdańska
wyświetlono 127 razy
Publikacje, które mogą cię zainteresować
Silver nanoparticles induce premutagenic DNA oxidation that can be prevented by phytochemicals from Gentiana asclepiadea
- A. HUDECOVá,
- B. Kusznierewicz,
- E. RUNDéN-PRAN
- + 16 autorów
Chromatographic analyses of Lavandula angustifolia and Rosmarinus officinalis extracts and their biological effects in mammalian cells and cell-free systems
- E. Horvathova,
- K. Kozicsa,
- M. Melusowa
- + 5 autorów
Folding‐upon‐repair DNA nanoswitches for monitoring DNA repair enzymes activity
- N. Farag,
- R. Mattossovich,
- R. Merlo
- + 5 autorów