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Wyniki wyszukiwania dla: CRISPR-CAS9
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Establishing the allosteric mechanism in CRISPR‐Cas9
PublikacjaAllostery is a fundamental property of proteins, which regulates biochemical information transfer between spatially distant sites. Here, we report on the critical role of molecular dynamics (MD) simulations in discovering the mechanism of allosteric communication within CRISPR‐Cas9, a leading genome editing machinery with enormous promises for medicine and biotechnology. MD revealed how allostery intervenes during at least three...
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Principles of target DNA cleavage and the role of Mg2+ in the catalysis of CRISPR–Cas9
PublikacjaAt the core of the CRISPR–Cas9 genome-editing technology, the endonuclease Cas9 introduces site-specific breaks in DNA. However, precise mechanistic information to ameliorate Cas9 function is still missing. Here, multimicrosecond molecular dynamics, free energy and multiscale simulations are combined with solution NMR and DNA cleavage experiments to resolve the catalytic mechanism of target DNA cleavage. We show that the conformation...
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CRISPR Journal
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Catalytic Mechanism of Non-Target DNA Cleavage in CRISPR-Cas9 Revealed by Ab Initio Molecular Dynamics
PublikacjaCRISPR-Cas9 is a cutting-edge genome editing technology, which uses the endonuclease Cas9 to introduce mutations at desired sites of the genome. This revolutionary tool is promising to treat a myriad of human genetic diseases. Nevertheless, the molecular basis of DNA cleavage, which is a fundamental step for genome editing, has not been established. Here, quantum–classical molecular dynamics (MD) and free energy methods are used...
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Crisia
Czasopisma -
Anti-crisis activities and export performance in the Covid-19 pandemic: The case of Polish exporters
PublikacjaThe article aims to investigate the impact of anti-crisis activities undertaken by the Polish exporting firms on their export sales during the Covid-19 pandemic. We used a quantitative research design. We conducted the survey on the sample of 161 manufacturing Polish exporting firms between April 21 and June 25, 2021. To verify the assumed relationships, we used the probit regression model. The main novelty is a firm-level analysis...
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Crisis – The Journal of Crisis Intervention and Suicide Prevention
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Economic Crisis in Croatia
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Economic crisis in Croatia
PublikacjaCroatia is becoming the 28th member of the European Union on July 1st, 2013. Croatia has gone a long way from a socialistic republic to an independent country recognized as one of the economic tigers of the Western Balkans in the first decade of the 21st century. Croatia has been hit by a global crisis which turned out to be a huge external shock for the region of the Western Balkans. Although it does not enter the economy through...
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Boiling crisis - danger or a must?
PublikacjaW artykule przedstawiono zjawisko kryzysu wrzenia w przepływie. W głównej mierze skupiono się na zjawisku dryout'u podczas przepływu pierścieniowego. W pracy przedstawiono teoretyczny model wysychania filmu cieczowego na ściance wraz z jego modyfikacją w oparciu o model dwurównaniowy zaprezentowany przez Sedlera i Mikielewicza (1981). Model ten opiera się na bilansie cieczy w rdzeniu przepływu i w filmie cieczowym, gdzie głównym...
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TQM tools in crisis management
PublikacjaThe current article is concerned with basic aspects connected with improvement of crisis management activities employed by economic operators. The work presents the crisis management model with its fundamental phases and identifies TQM tools that might be applied in all of these phases. The analysis included in the work has been supported with research conducted on companies located in northern Poland
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Illness Crisis and Loss
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Una crisi diversa?
PublikacjaIl capitolo tratta della recente crisi finanziaria, analizzandone cause e conseguenze per il sistema bancario e finanziario mondiale
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Headspace liquid phase microextraction for quantitation of hexanal in potato crisps by gas chromatography
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Case Study and Case Report
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Assessment of port facilities security in crisis management
PublikacjaZ punktu widzenia transportu międzynarodowego oraz przemysłowego charakteru systemu portowego, bezpieczeństwo obiektów portowych stanowi ważny element zarówno w zarządzaniu strategicznym portów morskich, jak również element ogólnej koncepcji zarządzania kryzysowego w ujęciu lokalnym, regionalnym, krajowym i międzynarodowym. W celu zapewnienia bezpieczeństwa portów morskich, muszą być prowadzone działania w celu przygotowania administracji...
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The trajectories of the financial crisis of companies at risk of bankruptcy
PublikacjaThis article concerns the assessment of the trajectory of the collapse of enterprises in Central Europe. The author has developed a model of a Kohonen artificial neural network. This model was used to determine 6 different classes of risk and was allowed to graphically determine the 5- to 10-year trajectory of going bankrupt. The study used data on 140 companies listed on the Warsaw Stock Exchange. This population was divided into...
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Digital Innovations for Sustainable Development in the Time of Crisis
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Business Risk Management in Times of Crises and Pandemics
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CRISIS MANAGEMENT PLANS IN CONTEXT OF CONTEMPORARY HAZARDS
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