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Search results for: BIOFILM, STAPHYLOCOCCUS, PROPOLIS
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Study of the process of biofilm formation by UPEC IH11128 in the M9+0,5% casamino acids medium
Open Research DataBiofilm formation is a direct reaction of bacteria to environmental factors. In order to determine the influence of the medium composition on the ability of IH11128 on biofilm production, we cultured bacteria under the following conditions: LB medium, M9 medium + 0,2% glucose, M9 + 0,5% casamino acids, M9 + 0,5% casamino acids + 0,2% glycerol, M9 +...
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Study of the process of biofilm formation by UPEC DR14 in the M9+0,5% casamino acids medium
Open Research DataBiofilm formation is a direct reaction of bacteria to environmental factors. In order to determine the influence of the medium composition on the ability of DR14 on biofilm production, we cultured bacteria under the following conditions: LB medium, M9 medium + 0,2% glucose, M9 + 0,5% casamino acids, M9 + 0,5% casamino acids + 0,2% glycerol, M9 + 0,5%...
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Novel staphylococci nucH taxonomical marker used in identification of human-associated Staphylococcus succinus subsp. casei
PublicationThe aim of our study was to assess the sequencing of unique nucH gene fragment based on performed bioinformatics analysis as a novel diagnostic method for the identification of difficult to identify staphylococcal human pathogenic strains. Initially, PCR-RFLP-rrn analysis specific to the spacers between 16SrDNA and 23SrDNA followed by HhaI restriction analysis was performed. Further, sequencing of nucH and 16S rDNA genes fragments...
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Molecular typing of Staphylococcus aureus isolated from cows with mastitis in the east of Poland on the basis of polymorphism of genes coding protein A and coagulase
PublicationPolimorfizm genów kodujących białko A i koagulazę wykorzystano do badania zmienności genetycznej bakterii Staphylococcus aureus wyizolowanych z gruczołów mlekowych krów u których stwierdzono stan zapalny. W badanej grupie 82 szczepów wyróżniono 24 genotypy.
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Intra-operative biopsy in chronic sinusitis detects pathogenic Escherichia coli that carry fimG/H, fyuA and agn43 genes coding biofilm formation
PublicationThe aim of this study was to investigate whether or not surgical biopsy of sinus tissue in chronic sinusitis, not responsive to treatment, would detect E. coli. We intended to evaluate E. coli virulence genes, therefore dispute the causal role of such an unusual microorganism in chronic sinusitis, as well as consider effective pathogen-targeted therapy. Patients with E. coli isolated by intra-operative puncture biopsy were included...
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Study of the process of biofilm formation by UPEC DR14 in the M9+0,5% casamino acids+0,2% glycerol medium
Open Research DataBiofilm formation is a direct reaction of bacteria to environmental factors. In order to determine the influence of the medium composition on the ability of DR14 on biofilm production, we cultured bacteria under the following conditions: LB medium, M9 medium + 0,2% glucose, M9 + 0,5% casamino acids, M9 + 0,5% casamino acids + 0,2% glycerol, M9 + 0,5%...
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Study of the process of biofilm formation by UPEC IH11128 in the M9+0,5% casamino acids+0,2% glucose medium
Open Research DataBiofilm formation is a direct reaction of bacteria to environmental factors. In order to determine the influence of the medium composition on the ability of IH11128 on biofilm production, we cultured bacteria under the following conditions: LB medium, M9 medium + 0,2% glucose, M9 + 0,5% casamino acids, M9 + 0,5% casamino acids + 0,2% glycerol, M9 +...
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Study of the process of biofilm formation by UPEC DR14 in the M9+0,5% casamino acids+0,2% glucose medium
Open Research DataBiofilm formation is a direct reaction of bacteria to environmental factors. In order to determine the influence of the medium composition on the ability of DR14 on biofilm production, we cultured bacteria under the following conditions: LB medium, M9 medium + 0,2% glucose, M9 + 0,5% casamino acids, M9 + 0,5% casamino acids + 0,2% glycerol, M9 + 0,5%...
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Study of the process of biofilm formation by UPEC IH11128 in the M9+0,5% casamino acids+0,2% glycerol medium
Open Research DataBiofilm formation is a direct reaction of bacteria to environmental factors. In order to determine the influence of the medium composition on the ability of IH11128 on biofilm production, we cultured bacteria under the following conditions: LB medium, M9 medium + 0,2% glucose, M9 + 0,5% casamino acids, M9 + 0,5% casamino acids + 0,2% glycerol, M9 +...
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Evaluation of antimicrobial activities of powdered cuttlebone against Klebsiella oxytoca, Staphylococcus aureus, and Aspergillus flavus
PublicationBackground: The presence of medicines in the environment is considered as a serious threat to the human health. The entrance of these substances into the water sources causes soil pollution, which eventually leads to the environmental pollution and it creates some problems for the public health. Also, increasing antibiotic resistant bacteria has attracted the attention of researchers to the use of natural resources such as marine...
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Effect of C-1748 derivatives on the biofilm formation of C. albicans ATCC 10231 cells
Open Research DataThe datasets contain the results of the impact of five C-1748 derivatives (IKE28 - IKE32) on the biofilm formation of Candida albicans strain ATCC 10231 cells. Photographic documentation prepared with the TECAN Spark 10M titration plate reader.
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Comprehensive methodology for Staphylococcus aureus lipidomics by liquid chromatography and quadrupole time-of-flight mass spectrometry
PublicationStaphylococcus aureus is a common pathogen known to cause relatively minor infections as well as severe disorders in humans. Although there is fair amount of published data concerning various aspects of its biology, epidemiology, genetics, etc., there is still a scarce amount of data presenting reliable and thorough investigations regarding high-throughput analysis of total S. aureus lipid content. Therefore, the aim of this study...
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Chemical composition of Polish propolis and its antiproliferative effect in combination with Bacopa monnieri on glioblastoma cell lines
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Method development and validation for optimized separation of the major polyphenolics in propolis extracts using GC-MS method
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Polylactide-Based Films with the Addition of Poly(ethylene glycol) and Extract of Propolis—Physico-Chemical and Storage Properties
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A study on the use of the BioBall® as a biofilm carrier in a sequencing batch reactor
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Optimization of Quantitative Analysis of Biofilm Cell from Pipe Materials
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Virulence gene profiles in Staphylococcus aureus isolated from cows with subclinical mastitis in eastern Poland
PublicationStaphylococcus aureus is arguably the most important pathogen involved in bovine mastitis. The aim of this study was to determine the virulence gene profiles of 124 Staph. aureus isolates from subclinical mastitis in cows in eastern Poland. The presence of 30 virulence genes encoding adhesins, proteases and superantigenic toxins was investigated by PCR. The 17 different combinations of adhesin genes were identified. Occurrence...
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Investigation of the Effect of the Rocket's Suborbital Flight on Biofilm, Enzymes and Biosynthesis on Autonomous, Modular and Scalable Platforms For Conducting Experiments of an Astrobiotechnological Nature
PublicationWith new incentives for human space exploration, biotechnological experiments in orbit became imperative. Answering this need, we build an autonomous, modular, and scalable platform that enables those experiments on rockets. We called it AMBER. The aim of the payload of the R6 suborbital rocket is to perform an experiment to study the influence of rocket flights on biofilm, molecular biology enzymes and biosynthesis using our platform....
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The combined effect of moderate pressure and chitosan on Escherichia coli and Staphylococcus aureus cells suspended in a buffer and on natural microflora of apple juice and minced pork
PublicationW pracy określono przeżywalność bakterii Escherichia coli i Staphylococcus aureus oraz naturalnej mikroflory soku jabłkowego i rozdrobnionego mięsa świńskiego po działaniu ciśnienia 193 MPa w temp. -20 C w obecności chitozanu. Stwierdzono, że traktowanie mikroorganizmów ciśnieniem w obecności chitozanu pozwala na przedłużenie trwałości badanych produktów przechowywanych w temperaturze chłodniczej o około 8-10 dni.
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Untargeted Lipidomics Reveals Differences in the Lipid Pattern among Clinical Isolates of Staphylococcus aureus Resistant and Sensitive to Antibiotics
PublicationStaphylococcus aureus resistance to antibiotics is a significant clinical problem worldwide. In this study, an untargeted lipidomics approach was used to compare the lipid fingerprints of S. aureus clinical isolates that are resistant and sensitive to antibiotics. High-performance liquid chromatography coupled with time-of-flight mass spectrometry was employed to rapidly and comprehensively analyze bacterial lipids. Chemometric...
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INVESTIGATION OF THE EFFECT OF THE ROCKET'S SUBORBITAL FLIGHT ON BIOFILM, ENZYMES AND BIOSYNTHESIS ON AUTONOMOUS, MODULAR AND SCALABLE PLATFORM FOR CONDUCTING EXPERIMENTS OF AN ASTROBIOTECHNOLOGICAL NATURE
PublicationWith new incentives for human space exploration, biotechnological experiments in orbit became imperative.Answering this need, we build an autonomous, modular, and scalable platform that enables those experiments onrockets. We called it AMBER. The aim of the payload of the R6 suborbital rocket is to perform an experiment tostudy the influence of rocket flights on biofilm, molecular...
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The agr function and polymorphism: Impact on Staphylococcus aureus susceptibility to photoinactivation
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Biochemical and Structural Characterization of SplD Protease from Staphylococcus aureus
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Occurrence and Characteristics of Staphylococcus aureus Isolated from Dairy Products
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Manipulation of Autophagy in Phagocytes Facilitates Staphylococcus aureus Bloodstream Infection
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Identification and characterization of Staphylococcus spp. and their susceptibility to insect apolipophorin III
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Biofilm formation as a virulence determinant of uropathogenic Escherichia coli Dr+ strains
PublicationInfekcje dróg moczowych stanowią największy problem zdrowotny dotykający miliony osób populacji ludzkiej każdego roku. Uropatogenne szczepy Escherichia coli są najczęstszym czynnikiem etiologicznym odpowiedzialnym za wywoływanie górnych i dolnych dróg moczowych. Powyższe szczepy bakteryjne produkują szereg czynników wirulentnych pośród, których wyróżnić można eksponowane powierzchniowo polimeryczne struktury adhezyjne (fimbrie/pile)...
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Synergistic effect of fennel essential oil and hydrogen peroxide on bacterial biofilm
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Cinnamaldehyde incorporated gellan/PVA electrospun nanofibers for eradicating Candida biofilm
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Effect of novel bisacridines (IKE16, IKE18, IKE19, IKE21) and IE10 on the biofilm formation of C. albicans ATCC 10231 cells
Open Research DataThe datasets contain the results of the impact of novel bisacridines (IKE16, IKE18, IKE19, IKE21) and IE10 on the biofilm formation of Candida albicans strain ATCC 10231 cells. Photographic documentation prepared with the TECAN Spark 10M titration plate reader.
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Significance of polymethylmethacrylate (PMMA) modification by zinc oxide nanoparticles for fungal biofilm formation
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The FT-IR and Raman Spectroscopies as Tools for Biofilm Characterization Created by Cariogenic Streptococci
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Mechanism of Biofilm Formation on Installation Materials and Its Impact on the Quality of Tap Water
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Association between susceptibility to photodynamic oxidation and the genetic background of Staphylococcus aureus
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Structural Determinants of Substrate Specificity of SplF Protease from Staphylococcus aureus
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The Bactericidal Activity of Temporin Analogues Against Methicillin Resistant Staphylococcus aureus
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Substrate specificity of Staphylococcus aureus cysteine proteases – Staphopains A, B and C
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A five-year retrospective study shows increasing rates of antimicrobial drug resistance in Cabo Verde for both Staphylococcus aureus and Escherichia coli
PublicationObjectives Data on baseline drug resistance is important in informing future antimicrobial stewardship programs. So far, no data on the antimicrobial drug resistance of clinical isolates was available for the African archipelago of Cabo Verde. Methods We have performed a retrospective analysis over five-years (2013-17) of the antimicrombial drug susceptibility profiles of clinical isolates in the two main hospitals of Cabo Verde....
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Evaluation of the Role of the Pharmacological Inhibition of Staphylococcus aureus Multidrug Resistance Pumps and the Variable Levels of the Uptake of the Sensitizer in the Strain-Dependent Response of Staphylococcus aureus to PPArg2-Based Photodynamic Ina
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Antimicrobial Activity of Selected Antimicrobial Peptides Against Planktonic Culture and Biofilm of Acinetobacter baumannii
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Application of Rotating Magnetic Fields Increase the Activity of Antimicrobials Against Wound Biofilm Pathogens
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Potential of Novel Bacterial Cellulose Dressings Chemisorbed with Antiseptics for the Treatment of Oral Biofilm Infections
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Acinetobacter baumannii isolated from hospital‐acquired infection: biofilm production and drug susceptibility
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Decolonization of Staphylococcus aureus in patients with atopic dermatitis: a reason for increasing resistance to antibiotics?
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Sub-lethal photodynamic inactivation renders Staphylococcus aureus susceptible to silver nanoparticles
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Silver Nanoparticles Combined With Naphthoquinones as an Effective Synergistic Strategy Against Staphylococcus aureus
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Tribenzoporphyrazines with dendrimeric peripheral substituents and their promising photocytotoxic activity against Staphylococcus aureus
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Possiblities of application of recombined lysostaphin as an alternative agent in the therapy of Staphylococcus aureus mastitis
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Detection of UPEC IH11128 ability to form biofilm in a standard congo red (CR) method of colony staining.
Open Research DataA standard method of testing E. coli's ability to form biofilm is based on the analysis of morphologyof bacterial colonies grown on yeast extract/casamino acids (YESCA) nutrient agar medium containing congored (CR) as an indicator dye of ECM production. The CR dye adsorbed from the culture medium bybacteria binds to the components of the ECM, including...