Search results for: SSDNA

• Molecular dynamics simulation and MM-PBSA analysis of Oxytricha nova TEBP ternary complex with ssDNA

  Publication

  • M. Wojciechowski
  • F. Fogolari
  • M. Bagiński

  - Year 2005

  Praca dotyczy badań metodami chemii obliczeniowej właściwości elektrostatycznych i termodynamicznych kompleksu telomerowego białka TEBP pochodzącego z organizmu Oxytricha z jednoniciowym telomerowym DNA (ssDNA).

• Novel thermostable ssDNA-binding proteins from Thermus thermophilus and T. aquaticus - expression and purification

  Publication

  • S. Dąbrowski
  • M. Olszewski
  • R. Piątek
  • J. Kur

  - Year 2005

  Białka SSB są niezbędne do życia każdemu organizmowi. W tej pracy prezentujemy wyniki otrzymania i oczyszczania białek SSB Thermus thermophilus i T. aquaticus. Dotąd były znane białka SSB jedynie o budowie homotetrameru lub heterotrimeru. Białka SSB Thermus thermophilus i T. aquaticus stanowią nową klasę białek SSB - białek homodimerycznych. Ponadto dowiedziono, iż białka te są użyteczne w reakcji PCR.

• Novel thermostable ssDNA-binding proteins from Thermus thermophilus and T. aquaticus-expression and purification

  Publication

  • S. Dąbrowski
  • M. Olszewski
  • R. Piątek
  • J. Kur

  - PROTEIN EXPRESSION AND PURIFICATION - Year 2002

  Sklonowano w system ekspresyjny pET-30LIC Escherichia coli gen kodujący białka SSB-podobne Thermus thermophilus i T. aquaticus. Oczyszczono do homogenności białka SSB-podobne, a uzyskane preparaty umożliwiają wykonanie badań strukturalnych w/w białek.

• CaZnO-based nanoghosts for the detection of ssDNA, pCRISPR and recombinant SARS-CoV-2 spike antigen and targeted delivery of doxorubicin

  Publication

  • N. Rabiee
  • O. Akhavan
  • Y. Fatahi
  • A. M. Ghadiri
  • M. Kiani
  • P. Makvandi
  • M. Rabiee
  • M. H. Nicknam
  • M. Saeb
  • R. S. Varma... and 4 others

  - CHEMOSPHERE - Year 2022

  Overexpression of proteins/antigens and other gene-related sequences in the bodies could lead to significant mutations and refractory diseases. Detection and identification of assorted trace concentrations of such proteins/antigens and/or gene-related sequences remain challenging, affecting different pathogens and making viruses stronger. Correspondingly, coronavirus (SARS-CoV-2) mutations/alterations and spread could lead to overexpression...

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• Characterization of a single-stranded DNA-binding-like Protein from Nanoarchaeum equitans - a nucleic acid binding protein with broad substrate specificity

  Publication

  • M. Olszewski
  • J. Balsewicz
  • M. Nowak
  • N. Maciejewska
  • A. Cyranka-Czaja
  • B. Zalewska-Piątek
  • R. Piątek
  • J. Kur

  - PLOS ONE - Year 2015

  Background SSB (single-stranded DNA-binding) proteins play an essential role in all living cells and viruses, as they are involved in processes connected with ssDNA metabolism. There has recently been an increasing interest in SSBs, since they can be applied in molecular biology techniques and analytical methods. Nanoarchaeum equitans, the only known representative of Archaea phylum Nanoarchaeota, is a hyperthermophilic, nanosized,...

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• RecA Proteins from Deinococcus geothermalis and Deinococcus murrayi - Cloning, Purification and Biochemical Characterisation

  Publication

  • M. Wanarska
  • B. Krawczyk
  • P. Hildebrandt
  • J. Kur

  - BMC MOLECULAR BIOLOGY - Year 2011

  Escherichia coli RecA plays a crucial role in recombinational processes, the induction of SOS response and mutagenic lesion bypasses. It has also been demonstrated that RecA protein is indispensable when it comes to the reassembly of shattered chromosomes in gamma-irradiated Deinococcus radiodurans, one of the most radiation-resistant organisms known. Moreover, some functional differences between E. coli and D. radiodurans RecA...

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• Identification and characterization of single-stranded DNA-binding protein from the facultative psychrophilic bacteria Pseudoalteromonas haloplanktis

  Publication

  • M. Olszewski
  • M. Nowak
  • A. Cyranka-Czaja
  • J. Kur

  - MICROBIOLOGICAL RESEARCH - Year 2014

  Single-stranded DNA-binding protein (SSB) plays an important role in DNA metabolism such as DNAreplication, repair, and recombination, and is essential for cell survival. This study reports on the ssb-likegene cloning, gene expression and characterization of a single-stranded DNA-binding protein of Pseudoal-teromonas haloplanktis (PhaSSB) and is the first report of such a protein from psychrophilic microorganism.PhaSSB possesses...

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• Identification and characterization of single-stranded-DNA-binding proteins from Thermus thermophilus and Thermus aquaticus - new arrangement of binding domains

  Publication

  • S. Dąbrowski
  • M. Olszewski
  • R. Piątek
  • A. Brillowska-Dąbrowska
  • G. Konopa
  • J. Kur

  - MICROBIOLOGY-SGM - Year 2002

  Określono molekularne właściwości białek SSB-podobnych z Thermus thermophi-lus i T. aquaticus. Zbadano aktywność białek oraz termostabilność. Ponadto, dowiedziono homodimeryczność obu białek i ustalono minimalną sekwencję wiążącą ssDNA.

• Characterization of single-stranded DNA-binding proteins from the psychrophilic bacteria

  Publication

  • M. Olszewski
  • M. Nowak
  • M. Skwarecka

  - Acta Biochimica Polonica - Year 2013

  Single-stranded DNA-binding proteins (SSBs) are indispensable elements in the cells of all living organisms. SSB proteins interact with ssDNA insequence in an independent manner, preventing them from forming secondary structures and from degradation by nucleases. In this way, SSB-binding proteins participate in all processes involving ssDNA, such as replication, repair and recombination. Although there are differences in amino...

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• Novel single-stranded DNA-binding proteins from extreme psychrophilic bacterium Psychromonas ingrahamii 37

  Publication

  • M. Olszewski
  • M. Nowak
  • N. Maciejewska
  • J. Kur

  - Year 2010

  We report the identification and characterization of the single-stranded DNA binding protein (SSB) from extreme psychrophilic bacterium Psychromonas ingrahamii 37 (PinSSB) that grows expotentially at -12°C and may well grow at even lower temperatures. PinSSB is one of the largest known bacterial SSB protein consisting 222 amino acid residues with a calculated molecular mass of 25.1 kDa. The analysis by gel filtration chromatography...

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• Characterization of single-stranded DNA-binding proteins from the psychrophilic bacteria

  Publication

  • M. Olszewski
  • M. Nowak
  • M. Skwarecka

  - Acta Biochimica Polonica - Year 2013

  Single-stranded DNA-binding proteins (SSBs) are indispensable elements in the cells of all living organisms. SSB proteins interact with ssDNAinsequence in an independent manner, preventing them from forming secondary structures and from degradation by nucleases. In this way, SSB-binding proteins participate in all processes involving ssDNA, such as replication, repair and recombination.Although there are differences in amino acid...

• Single-stranded DNA-binding proteins (SSBs) - sources and applicationsin molecular biology

  Publication

  • J. Kur
  • M. Olszewski
  • A. Długołęcka
  • P. Filipkowski

  - Year 2005

  Białka wiążące się do jednoniciowego DNA (SSB) odgrywają ważną rolę w replikacji, rekombinacji i naprawie DNA u bakterii, archea i eukariota. Ostatnio wzrosło zainteresowanie białkami SSB jako przydatnymi narzędziami w metodach badawczych biologii molekularnej, analitycznych i diagnostycznych. Większość znanych prokariotycznych białek SSB zbudowanych jest z czterech podjednostek (homotetramer) z jedną domeną wiążąca (OB) jednoniciowe...

• Novel primosomal protein B from thermophilic bacterium Thermoanerobacter tengcongensis

  Publication

  • M. (. Olszewski
  • M. Nowak
  • J. Kur

  - Acta Biochimica Polonica - Year 2011

  We report the identification and characterization of theprimosomal protein B (PriB) from thermophilic bacteriumThermoanerobacter tengcongensis (TtePriB). It is the largestknown bacterial PriB protein consisting 216 amino acidresidues with a calculated molecular mass of 25 kDa. Surprisingly,it is functional as monomer containing two single-stranded DNA binding domain (OB-fold) and it is thecompletely new kind structure of SSB protein....

• Multisine impedimetric probing of biocatalytic reactions for label-free detection of DEFB1 gene: How to verify that your dog is not human?

  Publication

  • P. Niedziałkowski
  • P. Ślepski
  • J. Wysocka
  • J. Chamier-Ciemińska
  • Ł. Burczyk
  • M. Sobaszek
  • A. Wcisło
  • T. Ossowski
  • R. Bogdanowicz
  • J. Ryl

  - SENSORS AND ACTUATORS B-CHEMICAL - Year 2020

  Albert is a dog (Canis familiaris), but he does not realize this. Albert loves human food (and beer), watching movies on the internet, sleeping in bed, and more. But he should not do all these things. To convince him that, we have desinged a test procedure. The DEFB1 gene is unique to human species. Detecting its presence from saliva and in short periods may offer an advantage in the field of forensic medicine, and influence Albert’s...

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• Synthesis of green benzamide-decorated UiO-66-NH2 for biomedical applications

  Publication

  • N. Rabiee
  • A. M. Ghadiri
  • V. Alinezhad
  • A. Sedaghat
  • S. Ahmadi
  • Y. Fatahi
  • P. Makvandi
  • M. Saeb
  • M. Bagherzadeh
  • M. Asadnia... and 2 others

  - CHEMOSPHERE - Year 2022

  Metal-organic frameworks (MOFs) biocompatible systems can host enzymes/bacteria/viruses. Herein we synthesized a series of fatty acid amide hydrolase (FAAH)-decorated UiO-66-NH2 based on Citrus tangerine leaf extract for drug delivery and biosensor applications. Five chemically manipulated FAAH-like benzamides were localized on the UiO-66-NH2 surface with physical interactions. Comprehensive cellular and molecular analyses were...

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• Characterization of exceptionally thermostable single-stranded DNA-binding proteins from Thermotoga maritima and Thermotoga neapolitana

  Publication

  • M. Olszewski
  • A. Grot
  • M. Wojciechowski
  • M. Nowak
  • M. Mickiewicz
  • J. Kur

  - BMC MICROBIOLOGY - Year 2010

  BACKGROUND: In recent years, there has been an increasing interest in SSBs because they find numerous applications in diverse molecular biology and analytical methods.RESULTS: We report the characterization of single-stranded DNA binding proteins (SSBs) from the thermophilic bacteria Thermotoga maritima (TmaSSB) and Thermotoga neapolitana (TneSSB). They are the smallest known bacterial SSB proteins, consisting of 141 and 142 amino...

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• Identification and properties of the Deinococcus grandis andDeinococcus proteolyticus single-stranded DNA binding proteins (SSB)

  Publication

  • P. Filipkowski
  • J. Kur

  - Acta Biochimica Polonica - Year 2007

  W celu poznania właściwości biochemicznych białek SSB pochodzących z Deinococcus grandis (DgrSSB) i Deinococcus proteolyticus (DprSSB) sklonowano zaamplifikowane w reakcji PCR geny ssb w systemie ekspresyjnym Escherichia coli. Geny składają się z 891(DgrSSB) i 876 (DprSSB) nukleotydów kodujących odpowiednio 296 i 291 reszt aminokwasowych o teoretycznie wyznaczonej masie cząsteczkowej dla białek, równej 32,29 i 31,33 kDa. Sekwencja...

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• Recombinant !ermostable AP Exonuclease from Thermoanaerobacter tengcongensis: Cloning, Expression, Purification, Properties and PCR Application

  Publication

  • S. Dąbrowski
  • A. Brillowska-Dąbrowska
  • B. Ahring

  - Polish Journal of Microbiology - Year 2013

  Apurinic/apyrimidinic (AP) sites in DNA are considered to be highly mutagenic and must be corrected to preserve genetic integrity, especially at high temperatures. !e gene encoding a homologue of AP exonuclease was cloned from the thermophilic anaerobic bacterium Thermoanaerobacter tengcongensis and transformed into Escherichia coli. The protein product showed high identity (80%) to human Ape1 nuclease, whereas to E. coli exonuclease...

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• Characterization of single-stranded DNA-binding proteins from the psychrophilic bacteria Desulfotalea psychrophila, Flavobacterium psychrophilum, Psychrobacter arcticus, Psychrobacter cryohalolentis, Psychromonas ingrahamii, Psychroflexus torquis, and Photobacterium profundum

  Publication

  • M. Nowak
  • M. Olszewski
  • M. Skwarecka
  • J. Kur

  - BMC MICROBIOLOGY - Year 2014

  Background: Single-stranded DNA-binding proteins (SSBs) play essential roles in DNA replication, recombination and repair in Bacteria, Archaea and Eukarya. In recent years, there has been an increasing interest in SSBs, since they find numerous applications in diverse molecular biology and analytical methods. Results: We report the characterization of single-stranded DNA-binding proteins from the psychrophilic bacteria Desulfotalea...

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• Techniki amplifikacji kwasów nukleinowych -2024-2025 - Nowy

  e-Learning Courses

  • B. Krawczyk
  • I. Sinkiewicz
  • A. Brillowska-Dąbrowska

  Specjalność: Biotechnologia molekularna (WCh), II stopnia, stacjonarne Prowadzący wykład: dr hab. Beata Krawczyk, prof. uczelni - stacjonarnie na uczelni Prowadzący laboratoria: dr hab. Beata Krawczyk, prof. uczelni - zajęcia stacjonarne na PG, sala 304 WCH B. Kontakt: (58) 347-23-83 Beata Krawczyk (wykłady): e-mail: beata.krawczyk@pg.edu.pl; WCH B; p.217; Konsultacje: stacjonarnie we wtorki o godzinie 13.  Opis kursu Kurs...

• Techniki amplifikacji kwasów nukleinowych -2022-2023

  e-Learning Courses

  • B. Krawczyk
  • I. Sinkiewicz

  Specjalność: Biotechnologia molekularna (WCh), II stopnia, stacjonarne Prowadzący wykład: dr hab. Beata Krawczyk, prof. uczelni - forma stacjonarna Prowadzący laboratoria: mgr inż. Bartosz Ostrowski - zajęcia stacjonarne na PG, sala 17 WCH C. Kontakt: (58) 347-23-83 Beata Krawczyk (wykłady): e-mail: beata.krawczyk@pg.edu.pl; WCH B; p.218; Bartosz Ostrowski, WCH B, p.218 tel. (58) 347-13-83 Konsultacje: stacjonarnie we wtorki ...

• Techniki amplifikacji kwasów nukleinowych -2021-2022

  e-Learning Courses

  • B. Krawczyk
  • I. Sinkiewicz
  • M. Burzyńska
  • A. Brillowska-Dąbrowska

  Specjalność: Biotechnologia molekularna (WCh), II stopnia, stacjonarne Prowadzący wykład: dr hab. Beata Krawczyk, prof. uczelni - stacjonarnie na uczelni Prowadzący laboratoria: mgr inż. Magdalena Fordon - zajęcia stacjonarne na PG, sala 17 WCH C. Kontakt: (58) 347-23-83 Beata Krawczyk (wykłady): e-mail: beata.krawczyk@pg.edu.pl; WCH B; p.218; Magdalena Fordon (laboratoria): magda.fordon@gmail.com; WCH B; p.218 Konsultacje:...

• Techniki Amplifikacji Kwasów Nukleinowych -2024-2025

  e-Learning Courses

  Specjalność: Biotechnologia molekularna (WCh), II stopnia, stacjonarne Prowadzący wykład: dr hab. Beata Krawczyk, prof. uczelni - forma stacjonarna Prowadzący laboratoria:  dr hab. Beata Krawczyk, prof. uczelni- zajęcia stacjonarne na PG, sala 304 WCH B (bud.7); III piętro. Kontakt: (58) 347-23-83 Beata Krawczyk (wykłady): e-mail: beata.krawczyk@pg.edu.pl; WCH B; p.217;tel. (58) 347-23-83 Bartosz Ostrowski, WCH B, p.218 tel....