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Search results for: CANDIDA KRUSEI
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Molecular detection of Candida krusei
PublicationThe species identification of fungi belonging to genus Candida is an important issue as this genus becomes the emerging problem of nosocomial infections. As Candida krusei presents intrinsicresistance to the fluconazole that is the drug of the first choice in case of invasive candidiasis the PCR identifying the DNA of C. krusei was elaborated. The analytical sensitivity of the assay on spiked blood samples was estimated at 3-5...
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Essential Oils, Silver Nanoparticles and Propolis as Alternative Agents Against Fluconazole Resistant Candida albicans, Candida glabrata and Candida krusei Clinical Isolates
PublicationDevelopment of effective and safe therapeutic treatment of fungal infections remains one of the major challenge for modern medicine. The aim of presented investigation was to analyze the in vitro antifungal activity of selected essential oils, ethanolic extracts of propolis and silver nanoparticles dropped on TiO2 against azole-resistant C. albicans (n = 20), C. glabrata (n = 14) and C. krusei (n = 10) clinical isolates. Among...
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Peptide Permeases in Candida albicans
PublicationAims: The aim of this work was to characterize oligopeptide uptake in Candida albicans mutants in which the genes encoding putative peptide permeases were selectively disrupted. Initial velocities of transport of model oligopeptides, (Ala)2, (Ala)3, (Ala)4, and sensitivity of mutants to antifungal oligopeptides containing N3-(4-methoxyfumaroyl)-L-2,3-diaminopropanoic acid (FMDP) were determined and compared to those of the wild-type...
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Virulence of Clinical Candida Isolates
PublicationThe factors enablingCandidaspp. infections are secretion of hydrolytic enzymes, adher-ence to surfaces, biofilm formation or morphological transition, and fitness attributes. The aim of thisstudy was to investigate the correlation between known extracellular virulence factors and survivalofGalleria mellonellalarvae infected with clinicalCandida. The 25 isolates were tested and the activityof proteinases among 24/24, phospholipases...
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Removal of cyclohexane and ethanol from air in biotrickling filters inoculated with Candida albicans and Candida subhashii
PublicationThis paper presents investigations on the removal of cyclohexane and ethanol from air in polyurethane- -packed biotrickling filters, inoculated with Candida albicans and Candida subhashii fungal species. Results on process performance together with flow cytometry analyses of the biofilm formed over packing elements are presented and discussed. The results indicate that the presence of ethanol enhances the removal efficiency of cyclohexane...
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Fibronectin-, vitronectin- and laminin-binding proteins at the cell walls of Candida parapsilosis and Candida tropicalis pathogenic yeasts
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Voriconazole and multidrug resistance in Candida albicans
PublicationPorównano aktywność grzybostatyczną in vitro worikonazolu, flukonazolu, ketokonazolu i klotrimazolu wobec opornych na flukonazol szczepów klinicznych Candida albicans oraz rekombinowanych szczepów Saccharomyces cerevisiae eksprymujących jeden z genów kodujących białka oporności wielolekowej (MDR) C. albicans: Cdr1p. Cdr2p lub CaMdr1p. Stwierdzono, że nadekspresja genów MDR czyni komórki drożdżaków mniej wrażliwymi na działanie...
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Characterization of two aminotransferases from Candida albicans
PublicationAminoadipate aminotransferase (AmAA) is an enzyme of α-aminoadipate pathway (AAP) for l-lysine biosynthesis. AmAA may also participated in biosynthesis or degradation of aromatic amino acids and in d-tryptophan based pigment production. The AAP is unique for fungal microorganisms. Enzymes involved in this pathway have specific structures and properties. These features can be used as potential molecular markers. Enzymes catalyzing...
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WIELOLEKOOPORNE ZAKAŻENIA CANDIDA - PROBLEM XXI WIEKU!
PublicationW ostatnich latach obserwuje się dramatyczny wzrost zakażeń grzybiczych, których przyczyną są grzyby z rodzaju Candida (C). Większość zakażeń wywoływana jest przez C. albicans, C. glabrata, C. krusei, C. parapsilosis i C. tropicalis. Dotychczas najczęściej stosowanymi lekami przeciwko Candida spp były azole, jednakże ze względu na występowanie naturalnej oporności na te antybiotyki (C. glabrata i C. krusei) coraz częściej azole...
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SKUTECZNOŚĆ DZIAŁANIA 5-FLUOROCYTOZYNY W STOSUNKU DO KLINICZNYCH IZOLATÓW CANDIDA ALBICANS I CANDIDA GLABRATA OPORNYCH NA FLUKONAZOL
PublicationCelem prowadzonych badań było sprawdzenie skuteczność działania 5-fluorocytozyny (5-FC) w stosunku do klinicznych izolatów C. albicans i C. glabrata wyizolowanych od pacjentów z trzech polskich szpitali. W przebadanej populacji wszystkie przebadane szczepy C. albicans były wrażliwe na działanie 5-FC, natomiast w przypadku gatunku C. glabrata wystąpiły 3 szczepy oporne i 4 średniooporne. Zjawisko nabywania lekooporności jest coraz...