Filters
total: 745
filtered: 527
-
Catalog
- Publications 527 available results
- Journals 30 available results
- Publishing Houses 1 available results
- People 20 available results
- Inventions 1 available results
- Projects 3 available results
- Research Teams 2 available results
- e-Learning Courses 7 available results
- Events 2 available results
- Open Research Data 152 available results
Chosen catalog filters
Search results for: ROZBICIE GENU
-
Transcriptomic responses to wounding: meta-analysis of gene expression microarray data
PublicationBackground A vast amount of microarray data on transcriptomic response to injury has been collected so far. We designed the analysis in order to identify the genes displaying significant changes in expression after wounding in different organisms and tissues. This meta-analysis is the first study to compare gene expression profiles in response to wounding in as different tissues as heart, liver, skin, bones, and spinal cord, and...
-
C421 allele-specific ABCG2 gene amplification confers resistance to the antitumor triazoloacridone C-1305 in human lung cancer cells
PublicationGen ABCG2 charakteryzuje polimorficzność pozycji C341, która jest związana ze znacznym obniżeniem ekspresji tego genu i zdolności do transportu przez błonę komórkową. W pracy wykazaliśmy, że komórki raka płuc, które nabyły oporności na związek C-1305, zmieniły fenotyp z heterozygotycznego w odniesieniu do genu ABCG2 i wykazują amplifikację jedynie allelu C341 genu oraz zwiększoną zawartość mRNA i zmutowanego białka pompy ABCG2...
-
Extracellular secretion of Pseudoalteromonas sp. cold-adapted esterase in Escherichia coli in the presence of Pseudoalteromonas sp. components of ABC transport system
PublicationEnzymy lipolityczne, głównie lipazy i esterazy, stanowią cenną grupę biokatalizatorów wykorzystywanych w przemyśle. Coraz większe zainteresowanie tą klasą enzymów sprawiło, że poszukuje się alternatywnych źródeł ich pozyskiwania. W celu zbadania wpływu białek transportu ABC Pseudoalteromonas sp. 643A na ekspresję genu, sekrecję i aktywność esterazy EstA z tego mikroorganizmu w systemie ekspresyjnym E. coli skonstruowano układ do...
-
Różnicowanie C. albicans i C. dubliniensis w oparciu o polimorfizm genu syntazy homocytrynianowej z wykorzystaniem metody PCR RFLP
PublicationNajczęstszym czynnikiem etiologicznym zakażeń wywołanych przez drożdżaki z rodzaju Candida jest C. albicans. Gatunek ten posiada bardzo zbliżone cechy fenotypowe do C. dubliniensis co często wiąże się z błędną identyfikacją. Skutkuje to wtórnym zakażeniem wywołanym przez współwystepujący gatunek C. dubliniensis, który nabywa oporność na flikonazol stosowany w terapii C. albicans. Celem badań była konstrukcja układu diagnostycznego...
-
Zimnolubna β-D-galaktozydaza Pseudoalteromonas sp. 22b - identyfikacja genu, klonowanie, ekspresja w komórkach Escherichia coli, oczyszczanie i charakterystyka.
Publication.
-
Adaptive filtering of microarray gene expression data based on Gaussian mixture decomposition
Publication -
Proteins Structure Models in the Evaluation of Novel Variant (C.472_477del) in the MOCS2 Gene
Publication -
Optimised expression cassettes of hpt marker gene for biolistic transformation of Miscanthus sacchariflorus
Publication -
Association of ENAM gene single nucleotide polymorphisms with dental caries in Polish children
Publication -
Cloning of the thermostable alpha-amylase gene from Pyrococcus woesei in Escherichia coli.
PublicationGen kodujący alfa-amylazę z Pyrococcus woesei klonowano do plazmidów pET21d(+)lub pYTB2, którymi transformowano komórki Escherichia coli. Otrzymana rekombinantowa alfa amylaza wykazuje maksymalną aktywność przy pH 5.6 w temperaturze 95 stC i przejawia około 24 % początkowej aktywności nawet po 2 godz. inkubacji w 120 stC. Duża termostabilność otrzymanego preparatu świadczy o jego przydatności do enzymatycznego upłynniania skrobi.
-
Genome-Wide DNA Methylation and Gene Expression in Patients with Indolent Systemic Mastocytosis
PublicationMastocytosis is a clinically heterogenous, usually acquired disease of the mast cells with a survival time that depends on the time of onset. It ranges from skin-limited to systemic disease, including indolent and more aggressive variants. The presence of the oncogenic KIT p. D816V gene somatic mutation is a crucial element in the pathogenesis. However, further epigenetic regulation may also affect the expression of genes that...
-
Prognostic Significance of TOP2A Gene Dosage in HER-2-Negative Breast Cancer
Publication -
Germline mutations in theCHEK2 kinase gene are associated with an increased risk of bladder cancer
Publication -
Gene set enrichment for reproducible science: comparison of CERNO and eight other algorithms
Publication -
The role of FNDC5 gene expression in irisin level changes accompanying thyroid pathologies
Publication -
Research Trends of Vitamin D Metabolism Gene Polymorphisms Based on a Bibliometric Investigation
Publication -
Biologically Active Preparations from the Leaves of Wild Plant Species of the Genus Rubus
Publication -
Relationship between polymorphism in the CRH gene and the traits of body conformation of Salers cows
Publication -
Description of plants and assessment of chemical properties of three species from the Amelanchier genus
Publication -
The role of TGF superfamily gene expression in the regulation of folliculogenesis and oogenesis in mammals: a review
Publication -
The Relationship Between Gene Polymorphisms and Dipping Profile in Patients With Coronary Heart Disease
Publication -
Homocitrate synthase gene as a new molecular target in diagnostics of Candida spp. infections
PublicationNew potential molecular target which will be investigated during this research (homocitrate synthase gen) is the innovative target other than ribosomal DNA. This gene encodes enzyme present only in fungi and some Archaea what significantly increases specificity. It also reduces risk of contamination or influence of other factors (reaction with other DNA present in sample derived from bacteria or host cells) what excludes false...
-
Justification of quasi-stationary approximation in models of gene expression of a self-regulating protein
PublicationWe analyse a model of Hes1 gene transcription and protein synthesis with a negative feedback loop. The effect of multiple binding sites in the Hes1 promoter as well as the dimer formation process are taken into account. We consider three, possibly different, time scales connected with: (i) the process of binding to/dissolving from a binding site, (ii) formation and dissociation of dimers, (iii) production and degradation of Hes1...
-
Changes in gene methylation patterns in neonatal murine hearts: Implications for the regenerative potential
PublicationBackground The neonatal murine heart is able to regenerate after severe injury; this capacity however, quickly diminishes and it is lost within the first week of life. DNA methylation is an epigenetic mechanism which plays a crucial role in development and gene expression regulation. Under investigation here are the changes in DNA methylation and gene expression patterns which accompany the loss of regenerative potential. Results The...
-
Sensitivity, Specificity and Prioritization of Gene Set Analysis When Applying Different Ranking Metrics
Publication -
Association between uridin diphosphate glucuronosylotranserase 1A1 (UGT1A1) gene polymorphism and neonatal hyperbilirubinemia
Publication -
Liver steatosis correlates with iron overload but not with HFE gene mutations in chronic hepatitis C
Publication -
Associations of ESR1 and ESR2 gene polymorphisms with metabolic syndrome and its components in postmenopausal women
Publication -
Morphogenesis-related gene-expression profile in porcine oocytes before and after in vitro maturation
Publication -
Evolving gene regulatory networks controlling foraging strategies of prey and predators in an artificial ecosystem
PublicationCo-evolution of predators and prey is an example of an evolutionary arms race, leading in nature to selective pressures in positive feedback. We introduce here an artificial life ecosystem in which such positive feedback can emerge. This ecosystem consists of a 2-dimensional liquid environment and animats controlled by evolving artificial gene regulatory networks encoded in linear genomes. The genes in the genome encode chemical...
-
Prognostic Value of TOP2A Gene Amplification and Chromosome 17 Polysomy in Early Breast Cancer
Publication -
Metabolic Gene Expression in the Muscle and Blood Parameters of Broiler Chickens Stimulated In Ovo with Synbiotics
Publication -
Plants of the Genus Terminalia: An Insight on Its Biological Potentials, Pre-Clinical and Clinical Studies
Publication -
Cellular and Gene Expression Response to the Combination of Genistein and Kaempferol in the Treatment of Mucopolysaccharidosis Type I
Publication -
TGF-β gene polimorphisms as risk factors for asthma control among clinic patients
Publication -
TGF-β gene polimorphisms as risk factors for asthma control among clinic patients.
Publication -
TGF-β gene polimorphisms as risk factors for asthma control among clinic patients.
Publication -
A review of the traditional uses, phytochemistry, pharmacology, and clinical evidence for the use of the genus Alchemilla (Rosaceae)
Publication -
The effects of p53 gene inactivation on mutant proteome expression in a human melanoma cell model
Publication -
Taxonomic Position and Phylogeny of the Genus Vargasiella (Orchidaceae, Vandoideae) Based on Molecular and Morphological Evidence
Publication -
Graphene-encapsulated iron nanoparticles as a non-viral vector for gene delivery into melanoma cells
Publication -
Expression of Deinococcus geothermalis Trehalose Synthase Gene in Escherichia coli and Its Enzymatic Properties
PublicationA novel trehalose synthase gene from Deinococcus geothermalis (DSMZ 11300) containing 1,692 bp reading-frame encoding 564 amino acids was amplified using PCR. The gene was ligated into pET30Ek/LIC vector and expressed after isopropyl alfa-D-thiogalactopyranoside induction in Escherichia coli BL21(DE3)pLysS. The recombinant trehalose synthase (DgeoTreS) containing a His6 tag at the C-terminus was purified by metal affinity chromatography...
-
Molecular identification and genotyping of Staphylococci: genus, species, strains, colnes, lineages, and interspecies exchanges
PublicationStaphylococci are increasingly recognized as etiological agents of many opportunistic human and animal infections, emphasizing the need for a rapid and accurate identification, even to a genotypical level of these bacteria. In the recent years, there has been a significant progress in typing and phylogenetic study of Staphylococcus species. Here, we describe molecular methods used in taxonomy as well as staphylococci characterization....
-
New, fast and cheap prediction tests for BRCA1 gene mutations identification in clinical samples.
PublicationDespite significant progress in cancer therapy, cancer is still the second cause of mortality in the world. The necessity to make quick therapeutic decisions forces the development of procedures allowing to obtain a reliable result in a quick and unambiguous manner. Currently, detecting predictive mutations, including BRCA1, is the basis for effectively treating advanced breast cancer. Here, we present new insight on gene mutation...
-
Evidence of adaptation, niche separation and microevolution within the genus Polaromonas on Arctic and Antarctic glacial surfaces
Publication -
p53 Gene status in relation to ex vivo chemosensitivity of non-small cell lung cancer
Publication -
Single median maxillary central incisor syndrome and variant in SMO gene associated with SHH pathway
Publication -
Application of PCR-HRM method for microsatellite polymorphism genotyping in the LDHA gene of pigeons (Columba livia)
Publication -
P-666 Detecting gene variants correlated with MII oocytes developed during ovarian stimulation
Publication -
A new potential mode of cardiorenal protection of KLOTHO gene variability in type 1 diabetic adolescents
Publication