Influence of Urea and Dimethyl Sulfoxide on K-Peptide Fibrillation - Publikacja - MOST Wiedzy

Wyszukiwarka

Influence of Urea and Dimethyl Sulfoxide on K-Peptide Fibrillation

Abstrakt

Protein fibrillation leads to formation of amyloids—linear aggregates that are hallmarks of many serious diseases, including Alzheimer’s and Parkinson’s diseases. In this work, we investigate the fibrillation of a short peptide (K-peptide) from the amyloidogenic core of hen egg white lysozyme in the presence of dimethyl sulfoxide or urea. During the studies, a variety of spectroscopic methods were used: fluorescence spectroscopy and the Thioflavin T assay, circular dichroism, Fourier-transform infrared spectroscopy, optical density measurements, dynamic light scattering and intrinsic fluorescence. Additionally, the presence of amyloids was confirmed by atomic force microscopy. The obtained results show that the K-peptide is highly prone to form fibrillar aggregates. The measurements also confirm the weak impact of dimethyl sulfoxide on peptide fibrillation and distinct influence of urea. We believe that the K-peptide has higher amyloidogenic propensity than the whole protein, i.e., hen egg white lysozyme, most likely due to the lack of the first step of amyloidogenesis—partial unfolding of the native structure. Urea influences the second step of K-peptide amyloidogenesis, i.e., folding into amyloids.

Cytowania

  • 0

    CrossRef

  • 0

    Web of Science

  • 0

    Scopus

Słowa kluczowe

Informacje szczegółowe

Kategoria:
Publikacja w czasopiśmie
Typ:
artykuły w czasopismach
Opublikowano w:
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES nr 23,
ISSN: 1661-6596
Język:
angielski
Rok wydania:
2022
Opis bibliograficzny:
Wawer J., Karczewski J., Aranowski R., Piątek R., Augustin-Nowacka D., Bruździak P.: Influence of Urea and Dimethyl Sulfoxide on K-Peptide Fibrillation// INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES -Vol. 23,iss. 6 (2022), s.3027-
DOI:
Cyfrowy identyfikator dokumentu elektronicznego (otwiera się w nowej karcie) 10.3390/ijms23063027
Weryfikacja:
Politechnika Gdańska

wyświetlono 57 razy

Publikacje, które mogą cię zainteresować

Meta Tagi