Rapid methods for microorganism quantification in human milk: Possibilities and evaluation

Katarzyna Mazur; Edyta Malinowska-Pańczyk; Izabela Drążkowska; Magdalena Chmara

doi:10.1016/j.idairyj.2025.106223

Rapid methods for microorganism quantification in human milk: Possibilities and evaluation

Abstrakt

This study evaluates the potential of rapid microbiological methods, traditionally used for food and environmental samples, to assess human milk (HM) quality in Human Milk Banks. Several methods, including a bioluminescence, the Micro Biological Survey (MBS) colorimetric method, triphenyltetrazolium chloride (TTC) reductase test, and flow cytometry (BacSomatic™️), were compared to the PN-EN ISO 4833–1:2013-12 reference method. Flow cytometry showed a strong correlation with the reference method for estimating bacterial counts in HM, indicating its potential as a fast alternative. Despite its speed − providing results in 10 min without pre-processing − flow cytometry showed some limitations suggesting it may not yet be suitable as a standalone tool. Other methods, such as TTC reduction test and bioluminescence, showed weaker correlations, indicating that external factors influence their accuracy, while flow cytometry is promising, further optimization is required before it can replace conventional methods.

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Słowa kluczowe

HUMAN MILK MICROBIOLOGICAL QUALITY FLOW CYTOMETRY BACTERIAL CONTAMINATION HUMAN MILK BANKING MICROBIOLOGICAL SCREENING

Informacje szczegółowe

Kategoria:

Publikacja w czasopiśmie

Typ:

artykuły w czasopismach

Opublikowano w:

INTERNATIONAL DAIRY JOURNAL nr 166,
ISSN: 0958-6946

Język:

angielski

Rok wydania:

2025

Opis bibliograficzny:

Mazur K., Malinowska-Pańczyk E., Drążkowska I., Chmara M.: Rapid methods for microorganism quantification in human milk: Possibilities and evaluation// INTERNATIONAL DAIRY JOURNAL -,iss. 166 (2025),

DOI: