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Wyniki wyszukiwania dla: SAC
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Real-time generator of AIS/ARPA/GPS data
PublikacjaThe paper presents a real-time generator of AIS/ARPA/GPS position data in the standardized NMEA 0183 text format. Position data are produced by a set of various types of ships simulated on the Polish part of the Baltic Sea and are generated on defined IP/UDP addresses and RS-232 ports. In the simulation an extensive set of input parameters is taken into account including the number of particular types of ships, their dimensions,...
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Analysis of IMS/NGN call processing performance using phase-type distributions
PublikacjaThis work is a continuation of our research on the traffic model dedicated for design and analysis of the Next Generation Network (NGN), which is standardized for distribution of current and future multimedia services based on the IP Multimedia Subsystem (IMS). Our analytical and simulation models allow evaluation of mean Call Set-up Delay E(CSD) as well as mean Call Disengagement Delay E(CDD) in a single domain of IMS/NGN. Ensuring...
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Analysis of IMS/NGN call processing performance using G/G/1 queuing systems approximations
PublikacjaThe paper concerns the Next Generation Network (NGN), which is standardized for distribution of multimedia services based on the IP Multimedia Subsystem (IMS). To guarantee quality, accurate traffic models of IMS/NGN should be proposed. In the paper we compare results obtained with our analytical and simulation model of a single IMS/NGN domain, where various queuing systems are used in the analytical model (M/G/1 and approximations...
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Approximation of Message Inter-Arrival and Inter-Departure Time Distributions in IMS/NGN Architecture Using Phase-Type Distributions
PublikacjaCurrently it is assumed that requirements of the information society for delivering multimedia services will be satisfied by the Next Generation Network (NGN) architecture, which includes elements of the IP Multimedia Subsystem (IMS) solution. In order to guarantee Quality of Service (QoS), NGN has to be appropriately designed and dimensioned. Therefore, proper traffic models should be proposed and applied. This requires determination...
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Traffic model for evaluation of call processing performance parameters in IMS-based NGN
PublikacjaIn the modern world requirements for accurate and fast information distribution are becoming more and more important, which creates a strong necessity for appropriate telecommunication network architecture. Proposition of such an architecture is the Next Generation Network (NGN) concept, which in order to guarantee Quality of Service (QoS), should be correctly designed and dimensioned. For this reason proper traffic models must...
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Zagadnienia inżynierii ruchu w sieciach NGN bazujących na IMS
PublikacjaSpełnienie wymagań dotyczących szybkości i dokładności dostarczania informacji powoduje konieczność wprowadzenia i poprawnego zaprojektowania sieci telekomunikacyjnych następnej generacji. W tym celu należy wskazać i zastosować odpowiednie modele ruchowe i metody inżynierii ruchu, czemu poświęcono niniejszą pracę. W pracy zaprezentowano standaryzację i architektury koncepcji IMS (IP Multimedia Subsystem) oraz bazujących na niej...
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Analysis of IMS/NGN Call Processing Performance Using Phase-Type Distributions Based on Experimental Histograms
PublikacjaThe paper describes our further research done with the proposed analytical and simulation traffic models of the Next Generation Network (NGN), which is standardized for delivering multimedia services with strict quality and includes elements of the IP Multimedia Subsystem (IMS). The aim of our models of a single IMS/NGN domain is to evaluate two standardized call processing performance parameters, which appropriate values are very...
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Gain deficit effect in the fractional delay filter design by the window method
PublikacjaMetoda okien jest sposobem szybkiego i skutecznego projektowania filtrów ułamkowoopóźniających. Jednakże wzmocnienie tak zaprojektowanego filtru różni się od zakładanej wartości idealnej. To zjawisko, nazywane deficytem wzmocnienia, negatywnie wpływa na jakoś projektowanego filtru. Artykuł poświęcono wynikom badań oraz metodom korekcji tego problemu. Omówiono wpływ współczynnika korekcji deficytu wzmocnienia na charakterystykę...
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Message Inter-Arrival and Inter-Departure Time Distributions in IMS/NGN Architecture
PublikacjaCurrently is it assumed that requirements of the information society for delivering multimedia services will be satisfied by the Next Generation Network (NGN) architecture, which includes elements of the IP Multimedia Subsystem (IMS) solution. In order to guarantee Quality of Service (QoS), NGN has to be appropriately designed and dimensioned. Therefore, proper traffic models should be proposed and applied. This requires determination...
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Review of Diameter protocol implementations for realization of communication in NGN networks
PublikacjaIn the paper Diameter protocol as well as its applications in the context of Next Generation Network (NGN) are described. Diameter message content and Attribute-Value Pairs (AVPs) concept are presented. Critical review of open source Diameter implementations is carried out. The performed analysis takes into consideration programming language, availability, support and documentation aspects. Basing on the review project fulfilling...
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A nearly optimal fractional delay filter design with asymmetric window
PublikacjaW pracy przedstawiono wyniki badań nad zastosowaniem asymetrycznego okna wyodrębnionego zawczasu z filtru optymalnego. Pokazano, że takie podejście przy zastosowaniu dodatkowej korekcji wzmocnienia filtru zależnej od opóźnienia filtru pozwala na projektowanie filtrów prawie optymalnych. W efekcie zaproponowana metoda łączy prostotę metody okien z jakością porównywalną do jakości filtrów optymalnych. Efektywność zaprezentowanej...
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Quality Parameters in IMS/NGN Networks
PublikacjaThe Next Generation Network (NGN) architecture, including elements of the IP Multimedia Subsystem (IMS) concept, is a proposition of a telecommunication network dedicated to the needs of current and future information society. The main goal of NGN is to provide Quality of Service (QoS), for which proper network design is necessary with respect to among others standardized call processing performance parameters, including expected...
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Performance Models of a Multidomain IMS/NGN Service Stratum
PublikacjaThis paper quantifies call processing performance (CPP) of a multidomain IMS/NGN architecture, which was proposed to deliver current and future telecommunication services with strict quality requirements, independently of the transport network technologies. A realistic simulation model is used as a reference for evaluation of the analytical results, in which vari-ous types of queuing systems are applied to model the opera-tion...
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Variable Fractional Delay Filter Design Using a Symmetric Window
PublikacjaIn this paper a numerically efficient method for designing a nearly optimal variable fractional delay (VFD) filter based on a simple and well-known window method is presented. In the proposed method a single window extracted from the optimal filter with fixed fractional delay (FD) is divided into even and odd part. Subsequently, the odd part is discarded and symmetric even part of the extracted window is used to design a family...
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Design of a Multidomain IMS/NGN Service Stratum
PublikacjaThe paper continues our research concerning the Next Generation Network (NGN), which is standardized for delivering multimedia services with strict quality and includes elements of the IP Multimedia Subsystem (IMS). A design algorithm for a multidomain IMS/NGN service stratum is proposed, which calculates the necessary CSCF servers CPU message processing times and link bandwidths with respect to the given maximum values of mean...
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Conservation and diversity of MutS proteins
PublikacjaThe homologues of MutS, mismatch repair protein, exist in all prokaryotes, with the exception of Actinobacteria, Mollicutes and part of the Archaea. Multiple alignments of 316 MutS amino acid sequences from 169 species revealed conserved residues and sequence motifs distinguishing MutS homologues. All MutS homologues show high conservation within the ATPase domain. MutS1, the homologue responsible for DNA mismatch recognition,...
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A bifunctional chimeric protein consisting of MutS and beta-galactoside
PublikacjaPraca pokazuje konstrukcję DNA plazmidowego kodującego bifunkcjonalne białko chimeryczne złożone z MutS Thermus thermophilus i beta-galaktozydazy E.coli, optymalizację jego ekspresji i oczyszczania. Białko to testowano w metodzie wykrywania mutacji punktowych, wykorzystując kolorymetryczne mierzenie aktywności domeny reporterowej beta galaktozydazy.
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A bifunctional chimeric protein consisting of MutS and beta-galactosidase
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A simple modification of PCR thermal profile applied to evade persisting contamination
PublikacjaThe polymerase chain reaction (PCR), one of the most commonly applied methods of diagnostics and molecular biology has a frustrating downside known as the false positive signal or contamination. Several solutions to avoid and to eliminate PCR contaminations have been worked out to date but the implementation of these solutions to laboratory practice may be laborious and time consuming. A simple approach to circumvent the problem...
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Transcriptomic Effects of Estrogen Starvation and Induction in the MCF7 Cells. The Meta-analysis of Microarray Results
PublikacjaEstrogen is one of the most important signaling molecules which targets a number of genes. Estrogen levels regulate cell proliferation and a plethora of metabolic processes, which may interfere with a range of medical conditions and drug metabolism. The MCF7 breast cancer cell line, expressing the estrogen receptor α, is a well-studied model of cellular answer to estrogen. The aim of this study was to characterize transcriptomic...
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Religious and Sacred Poetry: An International Quarterly of Religion, Culture and Education
Czasopisma -
Badania nad wykorzystaniem rekombinantowego białka MutS do wykrywania muta-cji punktowych.**2002, ????s. ???rys. ??? tab. bibliogr.???poz. maszyn. Rozprawa doktorska 10.04.2002, P. Gdań. Wydz. Chem. Promotor: prof. dr hab. J. Kur.
Publikacja.
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Construction and purification of his6-Thermus thermophilus MutS protein
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Construction and purification of his6-Thermus thermophilus MutS protein.
PublikacjaGen mutS z termofilnej bakterii Thermus thermophilus zamplifikowano przy użyciu PCR, sklonowano i przeprowadzono jego ekspresję w E. coli. Rekombinantowe białko MutS zawierające oligohistydynową domenę na N-końcu oczyszczono w jednoetapowej procedurze przy wykorzystaniu chromatografii powinowactwaNi(2+). Zdolność do rozpoznania niekomplementarności białka his(6)-MutS potwierdzono w eksperymentach ochrony DNA przed trawieniem...
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In vitro affinity of Deinococcus radiodurans MutS towards mismatched DNA exceeds that of its orthologues from Escherichia coli and Thermus thermophilus
PublikacjaThe mismatch binding protein MutS is responsible for the recognition of mispaired and unpaired bases, which is the initial step in DNA repair. Among the MutS proteins most extensively studied in vitro are those derived from Thermus thermophilus, Thermus aquaticus and Escherichia coli. Here, we present the first report on the in vitro examination of DNA mismatch binding activity of MutS protein from Deinococcus radiodurans and confront...
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Resovia Sacra. Studia Teologiczno-Filozoficzne Diecezji Rzeszowskiej
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Simulation Model for Application of the SDN Concept in IMS/NGN Network Transport Stratum
PublikacjaThe paper presents a simulation model allowing examination of cooperation between two currently used telecommunication networks concepts: IP Multimedia Subsystem/Next Generation Network (IMS/NGN) and Software-Defined Networking (SDN). Application of the SDN architecture elements in IMS/NGN networks will enable unified control and management of transport resources for various transport technologies and equipment manufacturers. However,...
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Analytical Traffic Model for a Multidomain IMS/NGN Network Including Service and Transport Stratum
PublikacjaThis paper addresses the problem of modelling call processing performance (CPP) in a multidomain Next Generation Network (NGN) architecture including the elements of the IP Multimedia Subsystem (IMS) in service stratum and based on the Multiprotocol Label Switching (MPLS) technology in transport stratum. An analytical traffic model for such an architecture is proposed by integrating the formerly implemented submodels of service...
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Zastosowanie koncepcji SDN w warstwie transportowej sieci IMS/NGN
PublikacjaKoncepcja sieci Next Generation Network, wykorzystująca w warstwie usług architekturę IP Multimedia Subsystem, zakłada centralizację funkcji sterowania zasobami sieciowymi w warstwie transportowej. Analogiczne założenie jest podstawą koncepcji Software Defined Networks. Na podstawie tej analogii, zaproponowano integrację obu koncepcji, przedstawiono architekturę zintegrowanej sieci, scenariusze sterowania zasobami oraz przygotowano...
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Implementation of IMS/NGN Transport Stratum Based on the SDN Concept
PublikacjaThe paper presents the development and verification of software and a testbed aiming to demonstrate the ability of two telecommunication network concepts—Next Generation Network (NGN) and Software-Defined Networking (SDN)—to cooperate. The proposed architecture includes components of the IP Multimedia Subsystem (IMS) in its service stratum and of the SDN (controller and programmable switches) in its transport stratum, providing...
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Simulation Model of IMS/NGN with Transport Stratum Based on MPLS Technology
PublikacjaIn the paper we continue our research on traffic modelling in the Next Generation Network (NGN) architecture, which includes the IP Multimedia Subsystem (IMS) elements. A simulation model of a single NGN domain with transport stratum based on the Multiprotocol Label Switching (MPLS) technology is proposed, which allows evaluation of mean response time of this stratum. The simulation model is applied to verify the results obtained...
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Database for integration of information in distributed data exchange system elements of Border Guard
PublikacjaThe paper presents the database solution for integration of information in distributed data exchange system elements of the Polish Border Guard. The proposed database solution is described in the context of data exchange system elements which control position and store identification data of vessels (fishing, sports and sailing boats) and other suspicious objects on the territorial sea, sea-coast and the internal sea-waters controlled...
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Simulation model of IMS/NGN call processing performance
PublikacjaIn current telecommunications it is assumed that demands of the information society for quickly delivered services will be satisfied by the Next Generation Network (NGN) architecture, which includes IP Multimedia Subsystem (IMS) elements. To guarantee Quality of Service (QoS), proper design and dimensioning of NGN is absolutely necessary, for which appropriate models have to be proposed. As the NGN architecture is very complicated,...
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Call processing performance in multidomain IMS/NGN architecture
PublikacjaThe Next Generation Network (NGN) architecture, which bases on the IP Multimedia Subsystem (IMS) concept, is a proposition of a telecommunication network dedicated to the needs of the modern information society. The main goal of NGN is to provide Quality of Service (QoS), for which proper network design and dimensioning are necessary. This also requires appropriate traffic models, which should be efficient and not excessively complicated...
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Traffic Model of IMS/NGN Architecture with Transport Stratum Based on MPLS Technology
PublikacjaGrowing expectations for a fast access to information create strong demands for a universal telecommunication network architecture, which provides various services with strictly determined quality. Currently it is assumed that these requirements will be satisfied by Next Generation Network (NGN), which consists of two stratums and includes IP Multimedia Subsystem (IMS) elements. To guarantee Quality of Service (QoS) all NGN stratums...
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Simulation model for assessment of IMS-based NGN call processing performance
PublikacjaIn current telecommunications it is assumed that demands of information society for quickly delivered services will be satisfied by Next Generation Network (NGN) architecture, which includes IP Multimedia Subsystem (IMS) elements. To guarantee Quality of Service (QoS), proper design and dimensioning of NGN is absolutely necessary, for which appropriate models have to be proposed. As NGN architecture is very complicated, the most...
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Uwierzytelnienie i autoryzacja w systemie STRADAR
PublikacjaPrzedstawiono rozwiązanie serwera uwierzytelnienia i autoryzacji (AA) w rozproszonym systemie STRADAR, udostępniającym funkcjonalności dla prowadzenia działań operacyjnych Morskiego Oddziału Straży Granicznej. System umożliwia prezentację na stanowisku wizualizacji zdarzeń (SWZ) bieżącej i archiwalnej sytuacji na mapie (AIS, radary), obrazu z kamer, zdjęć, notatek, rozmów telefonicznych oraz plików i wiadomości tekstowych (SMS)...
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Emulator and simulator of Terma SCANTER and ARPA radar data server
PublikacjaThe software solutions presented in this paper generate real-time data compatible with ARPA radar standard as well as Terma SCANTER 2001 radar cooperating with Video Distribution and Tracking (VDT) server. Two different approaches to this problem are considered: emulation based on the data captured from real devices and simulation of objects on the sea. For both of them architecture, implementation details and functional test results...
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Centralny serwer w multimedialnym systemie dyspozytorsko-teleinformatycznym STRADAR
PublikacjaPrzedstawiono architekturę i najnowsze funkcje centralnego serwera (Centrum) w rozproszonym systemie monitorowania akwenów morskich opracowanym dla straży granicznej. System umożliwia prezentację bieżącej i archiwalnej sytuacji na mapie, obrazu z kamer, rozmów telefonicznych oraz plików i wiadomości tekstowych (SMS) wymienianych przez funkcjonariuszy straży granicznej. W artykule skupiono się na nowych funkcjonalnościach centrum,...
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Adjuvant, neoadjuvant, and experimental regimens in overcoming pancreatic ductal adenocarcinoma
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Sacrum et Decorum. Materiały i studia z historii sztuki sakralnej
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Conserved motifs of MutL proteins
PublikacjatThe MutL protein is best known for its function in DNA mismatch repair (MMR). However, there isevidence to suggest that MutL is not only the linker connecting the functions of MutS and MutH in MMR,but that it also participates in other repair systems, such as Very Short Patch (VSP), Base Excision (BER)and Nucleotide Excision Repair (NER). This study set out to identify the most highly conserved aminoacid sequence motifs in MutL...
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A Proposal for Using Modified Site-specific Recombination Systems for Making Insertions into a Chosen Chromosomal Sitein vivoBased on the Analysis of I Phage Integration
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Reducing the Number of Microlocations in Oligonucleotide Microchip Matrices by the Application of Degenerate Oligonucleotides
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A Colorimetric Microplate Assay for DNA-Binding Activity of His-Tagged MutS Protein
PublikacjaA simple microplate method was designed for rapid testing DNA-binding activity of proteins. The principle of the assay involves binding of tested DNA by his-tagged protein immobilized on a nickel-coated ELISA plate, following colorimetric detection of biotinylated DNA with avidin conjugated to horseradish peroxidase. The method was used to compare DNA mismatch binding activities of MutS proteins from three bacterial species. The...
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Sequence variants of p21 gene of the MRL/MpJ mouse: a possible association with the deficit of p21 protein and regenerative phenotype.
PublikacjaThe MRL/MpJ mouse is known for its enhanced regeneration abilities that manifested themselves by scarless ear-hole wound closure. The deficit of p21 found in the MRL is associated with the regenerative capacity as the p21 gene knockout in a mouse strain unrelated to the MRL mouse results in an ear-hole closure effect, similar to that observed in the MRL mouse. Cdkn1a gene encodes p21 protein, a cyclin-dependent kinase inhibitor,...
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CARDINAL REGENERATIVE FEATURES OF THE MRL MOUSE — AN UPDATE
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Between therapy effect and false-positive result in animal experimentation
PublikacjaDespite the animal models’ complexity, researchers tend to reduce the number of animals in experiments for expenses and ethical concerns. This tendency makes the risk of false-positive results, as statistical significance, the primary criterion to validate findings, often fails if testing small samples. This study aims to highlight such risks using an example from experimental regenerative therapy and propose a machine-learning...
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A simple modification to improve the accuracy of methylation-sensitive restriction enzyme quantitative polymerase chain reaction
PublikacjaDNA digestion with endonucleases sensitive to CpG methylation such as HpaII followed by polymerase chain reaction (PCR) quantitation is commonly used in molecular studies as a simple and inexpensive solution for assessment of region-specific DNA methylation. We observed that the results of such analyses were highly overestimated if mock-digested samples were applied as the reference.We determined DNA methylation levels in several...
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Understanding the capacitance of thin composite films based on conducting polymer and carbon nanostructures in aqueous electrolytes
PublikacjaIn this work electrochemical performance of thin composite films consisted of poly(3,4-ethylenedioxythiophene) (PEDOT), graphene oxide (GOx) and oxidized multiwalled carbon nanotubes (oxMWCNTs) is investigated in various sulphates (Li2SO4, Na2SO4, K2SO4, MgSO4) and acidic (H2SO4) electrolytes. Capacitance values, rate capability and cycling stability achieved for the composite layers are correlated with the electrolytes’ properties...