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- Publikacje 196 wyników po odfiltrowaniu
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Wyniki wyszukiwania dla: błękitna biotechnologia
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A cryptic ribosome binding site, false signals in reporter systems and avoidance of protein translation chaos
PublikacjaThe expression of reporter gene may be induced by activation of cryptic signalling sequences, as we found while constructing the mutS-lacZ fusion gene. We cloned the Escherichia coli lacZ gene encoding beta-galactosidase into a plasmid vector carrying the Thermus thermophilus mutS gene. The clones expected to produce beta-galactosidase as the C-terminal fusion were selected for the complementation of beta-galactosidase activity...
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Production of trehalose in a single step enzymatic reaction
PublikacjaThe aim of this study was isolation and cloning of trehalose synthase gene derived from extremophilic microorganisms Deinoccoci to the expressive E. coli vectors and its biosythesis in different hosts.
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Isolation and properties of recombinant trehalose synthase
PublikacjaBiosynthesis of trehalose is carried out via various matabolic pathways, including the use of trehalose synthase. Bacteria of the genus Deionococcus are microorganisms which produce trehalose synthase catalizing conversion of maltose into trehalose. In this work recombinant trehalose synthase from D.radiodurans was characterized.
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Proteases immobilized on nanomaterials for biocatalytic, environmental and biomedical applications: Advantages and drawbacks
PublikacjaProteases have gained significant scientific and industrial interest due to their unique biocatalytic characteristics and broad-spectrum applications in different industries. The development of robust nanobiocatalytic systems by attaching proteases onto various nanostructured materials as fascinating and novel nanocarriers has demonstrated exceptional biocatalytic performance, substantial stability, and ease of recyclability over...
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Interspecies distances between propionic acid degraders and methanogens in syntrophic consortia for optimal hydrogen transfer
PublikacjaA mixed culture from an anaerobic biowaste digester was enriched on propionate and used to investigate interspecies hydrogen transfer in dependence of spatial distances between propionate degraders and methanogens. From 20.3 mM propionate, 20.8 mM acetate and 15.5 mM methane were formed. Maximum specific propionate oxidation and methane formation rates were 49 and 23 mmol mg−1day−1, respectively. Propionate oxidation was inhibited by...
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Cloning and characterization of a novel cold-active glycoside hydrolase family 1 enzyme with beta-glucosidase, beta-fucosidase and beta-galactosidase activities.
PublikacjaBackground: Cold-active enzymes, sourced from cold-adapted organisms, are characterized by high catalytic efficiencies at low temperatures compared with their mesophilic counterparts, which have poor activity. This property makes them advantageous for biotechnology applications as it: (i) saves energy costs, (ii) shortens the times for processes operated at low temperatures, (iii) protects thermosensitive substrates or products...
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Production of tropane alkaloids in Hyoscyamus niger (black henbane) hairy roots grown in bubble-column and spray bioreactors
PublikacjaHairy root cultures of Hyoscyamus niger were cultivated in shake-flasks, a bubble-column bioreactor and a hybrid bubble-column/spray bioreactor and evaluated for alkaloid production.
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In vitro affinity of Deinococcus radiodurans MutS towards mismatched DNA exceeds that of its orthologues from Escherichia coli and Thermus thermophilus
PublikacjaThe mismatch binding protein MutS is responsible for the recognition of mispaired and unpaired bases, which is the initial step in DNA repair. Among the MutS proteins most extensively studied in vitro are those derived from Thermus thermophilus, Thermus aquaticus and Escherichia coli. Here, we present the first report on the in vitro examination of DNA mismatch binding activity of MutS protein from Deinococcus radiodurans and confront...
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cDNA fingerprint from the hepatopancreatic glands of pond snails (Lymnaea stagnalis) exposed to benzo[a]pyrene
PublikacjaIdentification of differentially expressed genes that could be potentially used as biomarkers of PAH exposure of common invertebrate animal (like freshwater snail) would be a valuable resource for investigators interested in toxicology and biomonitoring of aquatic environments. Therefore, the aim of this research was to investigate effects of waterborne benzo[a]pyrene (B[a]P) exposure on mRNA expression in the pond snail’s (Lymnaea...
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Characterization of d-xylose reductase, XyrB, from Aspergillus niger
PublikacjaD-xylose reductase is a member of the aldo-keto reductase family, and is involved in D-xylose and L-arabinose conversion through the Pentose Catabolic Pathway (PCP) in fungi. In this study, we biochemically characterized a newly identified second D-xylose reductase (XyrB) from Aspergillus niger. This NADPH-dependent reductase is able to efficiently convert D-xylose and L-arabinose, and it has the highest affinity for these sugars...
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A dependency between PRL gene polymorphism and breeding parameters in Polish Black-and-White Holstein-Friesian cows
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Direct cloning of heavy metal resistance genes from metagenomic DNA
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Effect of addition of buserelin acetate to the extender on motility and viability of bovine spermatozoa
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Correlation between chosen polymorphisms in genes coding 5HTT, 5HT2A and COMT with obesity.
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Relationship between polymorphism in the CRH gene and the traits of body conformation of Salers cows
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Flavonoid biosynthetic pathways in plants: Versatile targets for metabolic engineering
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Rapid eradication of bacterial phytopathogens by atmospheric pressure glow discharge generated in contact with a flowing liquid cathode
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Isolation and characterization of the restriction endonucleasePpel fromPhormidium persicinum
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Enhanced extraction of proteins using cholinium-based ionic liquids as phase-forming components of aqueous biphasic systems
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Hot spot identification by ligand-protein surface interactions mapping – in silico study
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Software tools for identification, visualization and analysis of protein tunnels and channels
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Evolution of functionally important compartments of proteins – what can we learn from studying epoxide hydrolases?
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Water tracing as an alternative method for tunnels exploration in macromolecules
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A bifunctional chimeric protein consisting of MutS and beta-galactosidase
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The construction of bifunctional fusion proteins consisting of MutS and GFP
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Discovery and characterization of RecA protein of thermophilic bacterium Thermus thermophilus MAT72 phage Tt72 that increases specificity of a PCR-based DNA amplification
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Reaction of different microorganisms to novel graphene-based nanomaterials
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Construction and Use of a Broad-Host-Range Plasmid Expressing the lamB Gene for Utilization of Bacteriophage λ Vectors in the Marine Bacterium Vibrio harveyi
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Bacterial SSB-like proteins of family Deinococcaceae - indentification, isolation, obtaining, purification and molecular characteristic
PublikacjaWiększość poznanych białek SSB jest aktywna w formie homotetramerycznej. Od 2002 roku znane są białka organizmów ekstremofilnych. Scharakteryzowano białka mikroorganizmów rodziny Deinococaceae. Uzyskane termostabilne białka stanowią atrakcyjną alternatywę w stosunku do znanych białek SSB w metodach diagnostyki molekularnej, technikach biologii molekularnej i analityce.
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A bifunctional chimeric protein consisting of MutS and beta-galactoside
PublikacjaPraca pokazuje konstrukcję DNA plazmidowego kodującego bifunkcjonalne białko chimeryczne złożone z MutS Thermus thermophilus i beta-galaktozydazy E.coli, optymalizację jego ekspresji i oczyszczania. Białko to testowano w metodzie wykrywania mutacji punktowych, wykorzystując kolorymetryczne mierzenie aktywności domeny reporterowej beta galaktozydazy.
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The enhancement of anti-staphylococcal potential of plant derived naphthoquinones as a result of combination with silver nanoparticles
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Fingerprinting, structure, and genetic relationships among selected accessions of blue honeysuckle (Lonicera caerulea L.) from European collections
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Bacterial cellulose as a support for yeast immobilization – Correlation between carrier properties and process efficiency
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Significant enhancement of citric acid production by Yarrowia lipolytica immobilized in bacterial cellulose-based carrier
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The construction of bifunctional fusion proteins consisting of MutS and GFP
PublikacjaSkonstruowano białka chimeryczne zawierające białko MutS z Thermus thermophilus i białko zielonej fluoresceiny GFP z Aequorea victoria, posiadające dla łatwiejszego oczyszczania domeny oligo-histydynowe na N- lub C-końcu. Fuzyjne białka rozpoznawały niekomplementarności w DNA podobnie do MutS T. thermophilus. Fluorescencyjne białka rozpoznające niekomplementarne DNA mogą być użyteczne w wykrywaniu jednonukleotydowych polimorfizmów...
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Microalgal strains from the Culture Collection of Baltic Algae (CCBA)
Dane BadawczeThe dataset contains information on the cyanobacterial and microalgal strains maintained at the Culture Collection of Baltic Algae (CCBA) at the Institute of Oceanography UG. The collection maintains cyanobacterial and algal strains isolated from the Baltic Sea and additionally several strains collected from a wide range of habitats. The culture collection...
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Filozofia - Bio - 21/22
Kursy Onlinenazwa przedmiotu: Filozofia - tryb studiów - stacjonarne - stopień studiów - I stopnia - inżynierskie - kierunek studiów: Biotechnologia(WCh)
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Filozofia - Bio - 22/23
Kursy Onlinenazwa przedmiotu: Filozofia - tryb studiów - stacjonarne - stopień studiów - I stopnia - inżynierskie - kierunek studiów: Biotechnologia(WCh)
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Milena Marycz dr inż.
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A universal method for the identification of genes encoding amatoxins and phallotoxins in poisonous mushrooms
PublikacjaABSTRACT Background. As the currently known diagnostic DNA targets amplified in the PCR assays for detection of poisonous mushrooms have their counterparts in edible species, there is a need to design PCR primers specific to the genes encoding amanitins and phallotoxins, which occur only in poisonous mushrooms. Objective. The aim of the study was testing of PCR-based method for detection of all genes encoding hepatotoxic cyclic...
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Anna Bartosiewicz mgr inż.
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Bartosz Rybacki
OsobyA scientist, engineer, graduate of molecular biotechnology, R&D of the Stardust project, coordinator of the AMBER rocket project, member of the Polish Astrobiological Society, Polish Rocketry Association, Innspace Team and PTAstrobio Working Group. Employee of the Blirt S.A.. Ad Astra!
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Analiza żywności 2021/2022
Kursy OnlineKurs przeznaczony jest dla studentów kierunku Biotechnologia, I stopień, semestr VII realizujących przedmiot Analiza żywności w roku akademickim 2021/2022.
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Typing of Scopulariopsis and Microascus fungi by Random Amplified Polymorphic DNA (RAPD)
PublikacjaOBJECTIVES Scopulariopsis species and their teleomorphs of the genus Microascus are commonly isolated from soil, decaying plant material and indoor environments. Moreover, certain Scopulariopsis and Microascus species are recognised as opportunistic human pathogens. Although most species can be identified by detailed morphological study, phenotypic characters appear to overlap in several cases and morphology seems to be insufficient...
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PODSTAWY EKONOMII - Biotechnologia zima 2022 2023 [PG_00037482]
Kursy OnlineKierunek: Biotechnologia (WCh), I stopnia - inżynierskie, stacjonarne, 2022/2023 - zimowy (obecnie sem. 3) , Wykład (W) , Godziny zajęć: czwartki, 7:30 - 9:00Data rozpoczęcia zajęć: 06.10.2022Częstotliwość: Co tydzieńLiczba zajęć w semestrze: 15 Zapraszam!
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Chemoterapeutyki przeciwnowotworowe - wykład, Biotechnologia leków, 2023/2024
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Biochemia - seminarium, Biotechnologia sem. VI, 2021/2022
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Oksana Bielikowa Biotechnologia II st I sem
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Biochemia - ćwiczenia, Biotechnologia sem. V, 2022/2023
Kursy OnlineCelem ćwiczeń jest przygotowanie studentów do sprawnego prowadzenia obliczeń biochemicznych, m.in. związanych z przygotowywaniem odpowiednich stężeń roztworów buforowych, roztworów do hodowli komórek, jak i badań szybkości i mechanizmów reakcji enzymatycznych.
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Biochemia - ćwiczenia, Biotechnologia sem. V, 2021/2022
Kursy Online