Search results for: TRANSCRIPTION
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Transcription-Austin
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Methodology and technology for the polymodal allophonic speech transcription
PublicationA method for automatic audiovisual transcription of speech employing: acoustic, electromagnetical articulography and visual speech representations is developed. It adopts a combining of audio and visual modalities, which provide a synergy effect in terms of speech recognition accuracy. To establish a robust solution, basic research concerning the relation between the allophonic variation of speech, i.e., the changes in the articulatory...
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Methodology and technology for the polymodal allophonic speech transcription
PublicationA method for automatic audiovisual transcription of speech employing: acoustic and visual speech representations is developed. It adopts a combining of audio and visual modalities, which provide a synergy effect in terms of speech recognition accuracy. To establish a robust solution, basic research concerning the relation between the allophonic variation of speech, i.e. the changes in the articulatory setting of speech organs for...
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Overview of the Virtual Transcription Laboratory Usage Scenarios and Architecture
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Material for Automatic Phonetic Transcription of Speech Recorded in Various Conditions
PublicationAutomatic speech recognition (ASR) is under constant development, especially in cases when speech is casually produced or it is acquired in various environment conditions, or in the presence of background noise. Phonetic transcription is an important step in the process of full speech recognition and is discussed in the presented work as the main focus in this process. ASR is widely implemented in mobile devices technology, but...
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The role of Snail1 transcription factor in colorectal cancer progression and metastasis
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Description of the Dataset Hanow – Praecepta de Arte Disputandi – Transcription and Photographs
PublicationThis article briefly characterises the “Hanow – Praecepta de arte disputandi – transcription and photographs” research dataset. The dataset was created based on photographs and transcriptions of the manuscript of the Latin lectures on the rules of effective discussion (the title of the manuscript: Praecepta de arte disputandi) by Michael Chris-toph Hanow (1695–1773), professor of Gdańsk Academic Gymnasium. The original document...
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Expression of Selected Epithelial-Mesenchymal Transition Transcription Factors in Endometrial Cancer
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DNAffinity: a machine-learning approach to predict DNA binding affinities of transcription factors
PublicationWe present a physics-based machine learning approach to predict in vitro transcription factor binding affinities from structural and mechanical DNA properties directly derived from atomistic molecular dynamics simulations. The method is able to predict affinities obtained with techniques as different as uPBM, gcPBM and HT-SELEX with an excellent performance, much better than existing algorithms. Due to its nature, the method can...
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The Phytoestrogen Genistein Modulates Lysosomal Metabolism and Transcription Factor EB (TFEB) Activation
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Multiple transcriptional factors regulate transcription of the rpoE gene in Escherichia coli under different growth conditions and when the lipopolysaccharide biosynthesis is defective.
PublicationThe RpoE sigma factor is essential for the viability of Escherichia coli. RpoE regulates extracytoplasmic functions including lipopolysaccharide (LPS) translocation and some of its non-stoichiometric modifications. Transcription of the rpoE gene is positively autoregulated by EσE and by unknown mechanisms that control the expression of its distally located promoter(s). Mapping of 5′ ends of rpoE mRNA identified five new transcriptional...
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Description of the Dataset Rhetoric at School – a Selection of the Syllabi from the Academic Gymnasium in Gdańsk – Transcription and Photographs
PublicationThe research dataset described in the article was based on photographs and transcription of a textual record from Latin syllabi for classes at the Gdańsk Academic Gymnasium. The syllabi concern the years 1645/1648/1652/1653. The original document is held in the collection of the Gdańsk Library of the Polish Academy of Sciences [reference number: Ma 3920 8o]. The collected research material can be used for studying the practical...
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The effect of cabbage juice and its active components on transcription factor Nrf2 in breast cell lines
PublicationObniżenie zagrożenia chorobami nowotworowymi w przypadku diety bogatej w kapustę wiąże się ze zdolnością bioaktywnych związków tego warzywa do indukowania enzymów ochronnych 2. fazy będacych pod kontrolą czynnika transkrypcyjnego Nrf2. Zbadano wpływ soków z kapusty na poziom Nrf2 w komórkach unieśmiertelnionych i nowotworowych linii odpowiednio MCF10A i MCF-7. W obu przypadkach obserwowano wzrost poziomu tego czynnika oraz jego...
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Hyponastic Leaves 1 Interacts with RNA Pol II to Ensure Proper Transcription of MicroRNA Genes
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New markers for regulation of transcription and macromolecule metabolic process in porcine oocytes during in vitro maturation
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Excess nitrogen responsive HvMADS27 transcription factor controls barley root architecture by regulating abscisic acid level
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Expression of zinc finger transcription factors (ZNF143 and ZNF281) in serous borderline ovarian tumors and low-grade ovarian cancers
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Expression of selected epithelial–mesenchymal transition transcription factors in serous borderline ovarian tumors and type I ovarian cancers
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GlaR (YugA)—a novel RpiR‐family transcription activator of the Leloir pathway of galactose utilization in Lactococcus lactis IL 1403
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Are 8-oxoguanine (8-oxoGua) and 5-hydroxymethyluracil (5-hmUra) oxidatively damaged DNA bases or transcription (epigenetic) marks?
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Recent advances in transcription regulation
e-Learning CoursesRecent advances in transcription regulation
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Drastically decreased transcription from CII-activated promoters is responsible for impaired lysogenization of the Escherichia coli rpoA341 mutant by bacteriophage λ
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Can mutations in the gene encoding transcription factor EKLF (Erythroid Krüppel-Like Factor) protect us against infectious and parasitic diseases?
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Hanow - Praecepta de arte disputandi - transcription and photographs
Open Research DataPraecepta de arte disputandi by Enlightenment Gdańsk scholar Michael Christoph Hanow (1695-1773) are a combination of rhetorical theory and practical tips on how to effectively conduct discussions.
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Replicating DNA by cell factories: roles of central carbon metabolism and transcription in the control of DNA replication in microbes, and implications for understanding this process in human cells
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Impact of heat shock transcription factor 1 on global gene expression profiles in cells which induce either cytoprotective or pro-apoptotic response following hyperthermia
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Wernsdorf - a biography of an 18th century scholar - transcription, photographs and partial translation
Open Research DataThe data set contains the transcription of a large passage of the Latin biography of the eighteenth-century philologist and theologian Gottlieb Wernsdorf (1717-1774), as well as photos of the entire print. A translation from Latin into English and Polish was added to the data set as well (that is, excerpts on the education of both Gottlieb himself,...
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Multicopy Suppressor Analysis of Strains Lacking Cytoplasmic Peptidyl-Prolyl cis/trans Isomerases Identifies Three New PPIase Activities in Escherichia coli That Includes the DksA Transcription Factor
PublicationConsistent with a role in catalyzing rate-limiting step of protein folding, removal of genes encoding cytoplasmic protein folding catalysts belonging to the family of peptidyl-prolyl cis/trans isomerases (PPIs) in Escherichia coli confers conditional lethality. To address the molecular basis of the essentiality of PPIs, a multicopy suppressor approach revealed that overexpression of genes encoding chaperones (DnaK/J and GroL/S),...
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Rhetoric at school - a selection of the syllabi from the Academic Gymnasium in Gdańsk - transcription and photographs
Open Research DataThe following data set comprises a transcription (in txt and docx formats) and photographs (in jpg format) of the selected records from the Latin-language teaching syllabi termed 'Typus' or 'Catalogus lectionum' (an abbreviated title). Rhetoric is the main thematic criterion behind this choice. Various topics related to rhetoric have been taught not...
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2 Latin letters by Georg Pauli (b.1586-d.1650) - transcription, translation and photographs
Open Research DataThe data set contains two Latin letters by Georg Pauli (b. 1586 – d. 1650) to his brother Adrian (d. 1622) in photographs, transcriptions, and translations into Polish and English. The first letter was sent by Georg from Gdańsk (formerly Danzig) in 1604 when he was still a student at the local Academic Gymnasium. The second one, in turn, was written...
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The database of localization and expression of aquaporin 3 (AQP3), aquaporin 7 (AQP7) and aquaporin 9 (AQP9) in the male reproductive system in cattle. Morphometric studies. Localization of zinc finger transcription factor GATA-4.
Open Research DataThe data present research results being part of the OPUS-22 project entitled “In search of new markers of male fertility in cattle. Aquaporins expression in the reproductive organs and sperm of the bulls (Bos taurus)” obtained from the National Science Center in Poland (grant no. 2021/43/B/NZ9/00204). The aim of this part of the project was (i) to determine...
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Transcriptional activity of epigenetic remodeling genes declines in keratinocytes after in vitro expansion
PublicationPURPOSE: In vitro expansion is an invaluable method to obtain keratinocytes in amounts necessary for effective transplantation therapies. In vitro cell culturing provokes questions concerning potential epigenetic alterations occurring in expanded cells in the context of usefulness for transplantation and safety. The purpose of this study was to investigate as to whether keratinocyte expansion is associated with changes in the activity...
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Salt Stress Reveals a New Role for ARGONAUTE1 in miRNA Biogenesis at the Transcriptional and Posttranscriptional Levels
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Is the Identification of SNP-miRNA Interactions Supporting the Prediction of Human Lymphocyte Transcriptional Radiation Responses?
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Immunophenotyping and transcriptional profiling of in vitro cultured human adipose tissue derived stem cells
PublicationAdipose-derived stem cells (ASCs) have become an important research model in regenerative medicine. However, there are controversies regarding the impact of prolonged cell culture on the ASCs phenotype and their differentiation potential. Hence, we studied 10 clinical ASCs replicates from plastic and oncological surgery patients, in six-passage FBS supplemented cultures. We quantified basic mesenchymal cell surface marker transcripts...
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The Escherichia coli RNA polymerase alpha subunit and transcriptional activation by bacteriophage lambda CII protein.
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Transcriptional profile of in vitro expanded human epidermal progenitor cells for the treatment of non-healing wounds
PublicationBackground Epidermal progenitor cells (EPCs) have been under extensive investigation due to their increasing potential of application in medicine and biotechnology. Cultured human EPCs are used in the treatment of chronic wounds and have recently became a target for gene therapy and toxicological studies. One of the challenges in EPCs culture is to provide a high number of undifferentiated, progenitor cells displaying high viability...
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Genome-Wide Identification of Dickeya solani Transcriptional Units Up-Regulated in Response to Plant Tissues From a Crop-Host Solanum tuberosum and a Weed-Host Solanum dulcamara
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Modelling gene expression of a self-regulating protein
PublicationWe analyze a model of gene transcription and protein synthesis. We take into account the number of sites on the protein’s promoter at which the protein’s dimers can bind blocking transcription of protein mRNA.
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Optimizing Medical Personnel Speech Recognition Models Using Speech Synthesis and Reinforcement Learning
PublicationText-to-Speech synthesis (TTS) can be used to generate training data for building Automatic Speech Recognition models (ASR). Access to medical speech data is because it is sensitive data that is difficult to obtain for privacy reasons; TTS can help expand the data set. Speech can be synthesized by mimicking different accents, dialects, and speaking styles that may occur in a medical language. Reinforcement Learning (RL), in the...
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Hopf bifurcation in time‐delayed gene expression model with dimers
PublicationWe study a mathematical model of gene transcription and protein synthesis with negative feedback. We consider a system of equations taking into account the formation of dimers (i.e., complex formed by two protein monomers), the way in which dimers bind to DNA and time delay in translation process. For the model consisting of three ordinary differential equations with time delay, we derive conditions for stability of the positive...
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Regulated assembly of lipopolysaccharide and sensing of its alterations in Escherichia coli
PublicationThis thesis describes mechanism of the regulation of the transcription of the rpoE gene encoding an essential RNA polymerase subunit in Escherichia coli. The RpoE regulates extracytoplasmic stress response regulon and is required to initiate transcription of genes, whose products are involvedin the folding of periplasmic proteins and synthesis and transport of outer membrane components. The transcriptional regulation of the rpoE...
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Data on solutions of Hes1 system
Open Research DataHes1 protein (hairy and enhancer of split 1) belongs to the helix-loop-helix (bHLH) family of transcription proteins, i.e. DNA-binding proteins in the promoter region or in another region where regulation of transcription processes occurs. The database collects data on solutions of the Hes1 systems with multiple binding sites and the dimer formation...
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Interaction of the conserved region 4.2 of sigma(E) with the RseA anti-sigma factor
PublicationEo-E RNA polymerase transcribes a regulon of folding factors for the bacterial envelope and is induced by physical and chemical stresses. The RseA anti-sigma factor inhibits the activity of Esigma(E) RNA, polymerase. It is shown here that the N-terminal portion of sigma(E), residues 1-153, binds core RNA polymerase. RseA interacts with residues 154-191 of sigma(E), a site that is homologous to region 4, the sigma factor binding...
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Bimodal classification of English allophones employing acoustic speech signal and facial motion capture
PublicationA method for automatic transcription of English speech into International Phonetic Alphabet (IPA) system is developed and studied. The principal objective of the study is to evaluate to what extent the visual data related to lip reading can enhance recognition accuracy of the transcription of English consonantal and vocalic allophones. To this end, motion capture markers were placed on the faces of seven speakers to obtain lip...
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Gracjana Klein-Raina dr hab.
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Epitaph of Bartholomew Wagner in St. Mary's Church in Gdańsk
Open Research DataThe data set concerns epigraphy. It refers to the epitaph placed in St. Mary’s Church in Gdańsk, that is dedicated to Bartholomew Wagner, city physician from 1562, who came to Gdańsk from Konigsberg. Data set contains one general photo of the epitaph, transcription of its text in Latin, its translation in Polish, and the biography of the deceased, also...
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Foundation text of St. Mary's Church in Gdańsk
Open Research DataThe data set concerns epigraphy. It refers to the medieval foundation preserved on the wall above the sacristy entrance in St. Mary’s Church in Gdańsk, which confirms that the foundation stone of the temple was laid on 28th of March 1343. The data set contains one general photo of the foundation text, transcription of its text in Latin and its Polish...
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Epitaph of John Schroeder in St. Mary's Church in Gdańsk
Open Research DataThe data set concerns epigraphy. It refers to the epitaph placed in St. Mary’s Church in Gdańsk, that is dedicated to John Schröder, son of Simon. John, as he was a single, erected this epitaph for himself. Data set contains one general photo of the epitaph, transcription of its text in Latin, its translation in Polish, and the biography of the deceased,...
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Epitaph of Henry Giese in St. Mary's Church in Gdańsk
Open Research DataThe data set concerns epigraphy. It refers to the epitaph placed in St. Mary’s Church in Gdańsk, that is dedicated to Henry Giese, a member of merchant family that came from Germany and settled down in Gdańsk in XVI century. Data set contains one general photo of the epitaph, transcription of its text in Latin, its translation in Polish, and the biography...