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Search results for: mcts
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Multimedia Communications, Services and Security MCSS. 10th International Conference, MCSS 2020, Preface
PublicationMultimedia surrounds us everywhere. It is estimated that only a part of the recorded resources are processed and analyzed. These resources offer enormous opportunities to improve the quality of life of citizens. As a result, of the introduction of a new type of algorithms to improve security by maintaining a high level of privacy protection. Among the many articles, there are examples of solutions for improving the operation of...
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MutS as a tool for mutation detection
PublicationArtykuł przeglądowy pokazujący różne możliwości zastosowania różnych form białka MutS Thermus thermophilus do wykrywania mutacji punktowych.
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Conservation and diversity of MutS proteins
PublicationThe homologues of MutS, mismatch repair protein, exist in all prokaryotes, with the exception of Actinobacteria, Mollicutes and part of the Archaea. Multiple alignments of 316 MutS amino acid sequences from 169 species revealed conserved residues and sequence motifs distinguishing MutS homologues. All MutS homologues show high conservation within the ATPase domain. MutS1, the homologue responsible for DNA mismatch recognition,...
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The impact of ICTs on women’s economic empowerment
PublicationIt is widely argued that ICTs enable the inclusion of low-skilled and traditionally marginalized groups, such as women, people with disabilities, and workers at the base of the pyramid (BoP), in the labor market. In this paper, we investigate the determinants of female participation in the labor market in developing countries with a focus on the impact of the use of ICTs on female labor force participation. We conduct a panel study...
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The Impact of ICTs on Women’s Economic Empowerment
PublicationIt is widely argued that ICTs enable the inclusion of low-skilled and traditionally marginalized groups, such as women, people with disabilities, and workers at the base of the pyramid (BoP), in the labor market. In this paper, we investigate the determinants of female participation in the labor market in developing countries with a focus on the impact of the use of ICTs on female labor force participation. We conduct a panel study...
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Banque des mots
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Concentration in ICTs sector - cross country analysis
PublicationIn the chapter below, the author present inequalities in sector of new information and communication technologies. Having in mind inequalities we can also discuss concentration issues, and the concentration itself is a problem to which the author refers on first place. The paper is organized as following: first basic measures of concentration are defined, after we can read a discussion referring to concentration in ICTs sector...
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MutS3: a MutS homologue of unknown biological function
PublicationThe homologues of MutS proteins are widespread among both Prokaryotes and Eukaryotes. MutS designated as MutS1 is a part of MMR (mismatch repair) system which is responsible for removal of mispaired bases and small insertion/deletion loops in DNA. Initially, the only MutS homologues known were those engaged in mismatch repair and these were later designated as MutS1. Subsequently, the MutS2 homologue was distinguished. MutS2 does...
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Construction and purification of his6-Thermus thermophilus MutS protein.
PublicationGen mutS z termofilnej bakterii Thermus thermophilus zamplifikowano przy użyciu PCR, sklonowano i przeprowadzono jego ekspresję w E. coli. Rekombinantowe białko MutS zawierające oligohistydynową domenę na N-końcu oczyszczono w jednoetapowej procedurze przy wykorzystaniu chromatografii powinowactwaNi(2+). Zdolność do rozpoznania niekomplementarności białka his(6)-MutS potwierdzono w eksperymentach ochrony DNA przed trawieniem...
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Construction and purification of his6-Thermus thermophilus MutS protein
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A bifunctional chimeric protein consisting of MutS and beta-galactosidase
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The construction of bifunctional fusion proteins consisting of MutS and GFP
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A bifunctional chimeric protein consisting of MutS and beta-galactoside
PublicationPraca pokazuje konstrukcję DNA plazmidowego kodującego bifunkcjonalne białko chimeryczne złożone z MutS Thermus thermophilus i beta-galaktozydazy E.coli, optymalizację jego ekspresji i oczyszczania. Białko to testowano w metodzie wykrywania mutacji punktowych, wykorzystując kolorymetryczne mierzenie aktywności domeny reporterowej beta galaktozydazy.
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The construction of bifunctional fusion proteins consisting of MutS and GFP
PublicationSkonstruowano białka chimeryczne zawierające białko MutS z Thermus thermophilus i białko zielonej fluoresceiny GFP z Aequorea victoria, posiadające dla łatwiejszego oczyszczania domeny oligo-histydynowe na N- lub C-końcu. Fuzyjne białka rozpoznawały niekomplementarności w DNA podobnie do MutS T. thermophilus. Fluorescencyjne białka rozpoznające niekomplementarne DNA mogą być użyteczne w wykrywaniu jednonukleotydowych polimorfizmów...
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Role of Dean’s Offices in Developing Internal Normative Acts at HEIs
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Wdrożenie ICTs a rozwój społeczeństwa informacyjnego w warunkach gospodarki rynkowej
PublicationArtykuł dotyczy zagadnień związanych z rozwojem społeczeństwa informacyjnego w poszczególnych krajach. Pokazano różne typy społeczeństwa informacyjnego ze względu na stadium jego rozwoju w kraju. Ponadto dokonano prostej analizy statystycznej zależności pomiędzy stopniem rozwoju społeczestwa informacyjnego a stopnień swobody prowadzenia działalności gospodarczej.
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Wdrożenie ICTs w krajach słabo rozwiniętych gospodarczo - analiza statystyczna
PublicationW artykule poddano analizie statystycznej relację pomiędzy poziomie rozwoju gospodarczego, a poziomiem wdrożenia nowoczesnych technologii informacyjnych i komunikacyjnych.
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An Approach to the Detection of Bank Robbery Acts Employing Thermal Image Analysis
PublicationA novel approach to the detection of selected security-related events in bank monitoring systems is presented. Thermal camera images are used for the detection of people in difficult lighting conditions. Next, the algorithm analyses movement of objects detected in thermal or standard monitoring cameras using a method evolved from the motion history images algorithm. At the same time, thermal images are analyzed in order to detect...
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MTS pilot tone tracking for "wow" distortion determination
PublicationArtykuł przedstawia nowy algorytm do wyznaczania charakterystyki kołysania dźwięku na podstawie śledzenia zmian tonu pilota MTS. Śledzenie zmian częstotliwości nośnej tonu MTS pozwala określić zmienną w czasie głębokość pasożytniczych modulacji częstotliwości będących konsekwencją kołysania. Na tej podstawie tworzona jest charakterystyka zniekształcenia. Proponowany algorytm bazuje na zaproponowanej wcześniej metodzie śledzenie...
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A Colorimetric Microplate Assay for DNA-Binding Activity of His-Tagged MutS Protein
PublicationA simple microplate method was designed for rapid testing DNA-binding activity of proteins. The principle of the assay involves binding of tested DNA by his-tagged protein immobilized on a nickel-coated ELISA plate, following colorimetric detection of biotinylated DNA with avidin conjugated to horseradish peroxidase. The method was used to compare DNA mismatch binding activities of MutS proteins from three bacterial species. The...
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Mots: Les langages du politique
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CTS-Clinical and Translational Science
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MCS-Masculinities and Social Change
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Preliminary studies on DNA retardation by MutS applied to the detection of point mutations in clinical samples
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Zastosowanie immobilizowanego białka MutS do wychwytywania fragmentów DNA zawierających niesparowane zasady
PublicationBiałko MutS wiąże DNA zawierające błędnie sparowane oraz niesparowane zasady w DNA. Podjęto szereg prób wykorzystania tego białka jako narzędzia do detekcji nieznanych mutacji lub zagęszczania frakcji DNA z niesparowaną zasadą. Dotychczas żadna z metod oparta na użyciu białka MutS nie została wdrożona powszechnie do praktyki laboratoryjnej. Głównym powodem takiego stanu rzeczy jest stosunkowo silne powinowactwo MutS do DNA w pełni...
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Snow cover and its variability in the Polish Sudetes Mts. and the Sudetic Foreland
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Large red cyanobacterial mats (Spirulina subsalsa Oersted ex Gomont) in the shallow sublittoral of the southern Baltic
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Duration of snow cover and its variability in the Polish part of the Sudetes Mts. and their foreland
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In vitro affinity of Deinococcus radiodurans MutS towards mismatched DNA exceeds that of its orthologues from Escherichia coli and Thermus thermophilus
PublicationThe mismatch binding protein MutS is responsible for the recognition of mispaired and unpaired bases, which is the initial step in DNA repair. Among the MutS proteins most extensively studied in vitro are those derived from Thermus thermophilus, Thermus aquaticus and Escherichia coli. Here, we present the first report on the in vitro examination of DNA mismatch binding activity of MutS protein from Deinococcus radiodurans and confront...
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Preferences Towards Working in Culturally Diverse Teams at Different Stages of the Entrepreneurial Process
PublicationAs entrepreneurial teams become prevalent, it is important to study factors related to their creation and effectiveness. The goal of the current research was to analyze preferences towards working in a multicultural team (MCT) versus a single culture team (SCT) at different stages of the entrepreneurial process. The study involved students originating from various cultures with previous experience in both SCTs and MCTs as participants....
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A New Phenylalanine Derivative Acts as an Antagonist at the AMPA Receptor GluA2 and Introduces Partial Domain Closure: Synthesis, Resolution, Pharmacology, and Crystal Structure
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Giving Moral Competence High Priority in Medical Education. New MCT-based Research Findings from the Polish Context
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The Jurassic–Cretaceous transition in the High-Tatric succession (Giewont Unit, Western Tatra Mts, Poland): integrated stratigraphy and microfacies
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Multimedia manual of cardiothoracic surgery : MMCTS / European Association for Cardio-Thoracic Surgery
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Tracing the latest Jurassic–earliest Cretaceous paleoenvironment evolution in swell carbonate facies: a case study of the High-Tatric succession (Central Western Carpathians, Tatra Mts, Poland)
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Internal legal acts of technical and medical universities in Poland regulating classes conducted in-person during the Covid-19 pandemic
Open Research DataA database of legal acts and other internal documents of medical and technical universities in Poland regulating the way of organizing in-person or hybrid classes during the COVID-19 pandemic from the summer semester 2019/2020 to the winter semester 2020/2021.Documents were encoded in two separate coding systems using the MAXQDA program for qualitative...
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Badania nad wykorzystaniem rekombinantowego białka MutS do wykrywania muta-cji punktowych.**2002, ????s. ???rys. ??? tab. bibliogr.???poz. maszyn. Rozprawa doktorska 10.04.2002, P. Gdań. Wydz. Chem. Promotor: prof. dr hab. J. Kur.
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Magdalena Brzozowska-Woś dr hab. inż.
PeopleMagdalena Brzozowska-Woś is a graduate of the Faculty of Management and Economics of the Gdańsk University of Technology (specialization: management systems). She is also a graduate of Postgraduate Studies in Advertising (Faculty of Management and Economics, GUT) and Postgraduate Studies in Public Relations (SWPS University of Humanities and Social Sciences). In the years 2000-2003, she cooperated with Panorama Internet sp. z o....
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Paweł Sachadyn prof. dr hab. inż.
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INFLUENCE OF A VERTEX REMOVING ON THE CONNECTED DOMINATION NUMBER – APPLICATION TO AD-HOC WIRELESS NETWORKS
PublicationA minimum connected dominating set (MCDS) can be used as virtual backbone in ad-hoc wireless networks for efficient routing and broadcasting tasks. To find the MCDS is an NP- complete problem even in unit disk graphs. Many suboptimal algorithms are reported in the literature to find the MCDS using local information instead to use global network knowledge, achieving an important reduction in complexity. Since a wireless network...
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Poszukiwanie sankcji optymalnej w perspektywie projektu ustawy z dnia 7 lipca 2022 r. o zmianie ustawy Kodeks karny oraz niektórych innych ustaw. The search for the optimal sanction in the perspective of the draft act of 7 July 2022 amending the Penal Code and some other acts.
PublicationPolski ustawodawca opublikował w połowie września 2021 r. na stronie Rządowego Centrum Legislacji projekt gruntownej zmiany – między innymi – Kodeksu karnego. W dniu 7 lipca 2022 r. Sejm RP przyjął ww. projekt ustawy (druk sejmowy nr 2024). W zasadniczym stopniu nowelizacja - między innymi - zwiększa wysokość kar grożących za poszczególne przestępstwa. Ustawodawca – poprzez zabiegi legislacyjne –...
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Education at the Faculty of Mechanical Engineering of the Gdańsk University of Technology
PublicationFaculty of Mechanical Engineering is one of the largest departments of the Gdańsk University of Technology (GUT), currently educating about 3 000 student. Lectures are conducted in five Chairs, which include 18 Divisions. In 2000 GUT introduced the European Credit Transfer and Accumulation System (ECTS). Each student should get during one academic year 60 ECTS points. Obtaining a diploma of BEng requires the acquisition of no less...
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Education at the Faculty of Mechanical Engineering of the Gdańsk University of Technology
PublicationFaculty of Mechanical Engineering is one of the largest departments of the Gdańsk University of Technology (GUT), currently educating about 3 000 student. Lectures are conducted in five Chairs, which include 18 Divisions. In 2000 GUT introduced the European Credit Transfer and Accumulation System (ECTS). Each student should get during one academic year 60 ECTS points. Obtaining a diploma of BEng requires the acquisition of no less...
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Technologie informacyjne i komunikacyjne w Polsce : stan obecny i perspektywy rozwoju
PublicationNowoczesne technologie informacyjne i komunikacyjne (icts - information and communication technologies) to jeden z najważniejszych elementów, który stanowi o stanie krajowej - jak i światowej - gospodarki. w literaturze można spotkać się z pew-ną dychotomią pojęciową icts. z jednej strony icts są traktowane jako sektor gospodarki, który dostarcza dóbr i usług it, przyczyniając się do wzrostu go-spodarczego i zmieniając strukturę...
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Digital divide - inequalities in level of implementation of new information and telecommunication technologies. Cross country study
PublicationTekst stanowi analizę nierówności stopnia wdrożenia oraz implementacji ICTs w różnych krajach świata. Autor szacuje wskaźnik GINI dla możliwych krajów świata.In the paper presented, the author considers few aspects of the so-called "digital divide". It is easily noticeable worldwide, that new information and communication technologies (ICTs) "possess" a great ability to spread, at high pace, among countries from all around the...
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Does technology matter for economic development? Empirical evidence for Latin American Countries
PublicationThe spread of new Information and Communication Technologies (ICTs) has been recognized worldwide. ICTs are broadly perceived as tools facilitating economic growth and development, especially in backward countries. They are easy and cheap to adopt, require minimum skills for effective use, and bring opportunities for disadvantaged societies. They enable education, knowledge dissemination and sharing, and processing and storing...
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Technology convergence and digital divides. A country-level evidence for the period 2000–2010
PublicationThe paper, mostly empirical in nature, investigates issues on cross-national new information and communication technologies (ICTs) adoption patterns and growth directions. In the period of 2000–2010, a great number of countries underwent substantial changes on the field of ICTs implementation. Many of them made a great “jump” starting with almost “zero level” of ICTs adoption in the year 2000 and during the ten- year period were...
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Przemysław Kalitowski dr inż.
PeoplePrzemyslaw Kalitowski (born 26 July 1991) currently works at the Gdansk University of Technology in Gdansk, Poland. He acts as the Professor's Assistant at the Department of Rail Transportation and Bridges. In 2015 he started PhD studies at the Department of Rail Transportation and Bridges. He has over 4 years experience in static and dynamic analysis of structures - primarily with FEM programs. The scope of analysis are mainly...
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A New Approach for the Mitigating of Flow Maldistribution in Parallel Microchannel Heat Sink
PublicationThe problem of flow maldistribution is very critical in microchannel heat sinks (MCHS). It induces temperature nonuniformity, which may ultimately lead to the breakdown of associated system. In the present communication, a novel approach for the mitigation of flow maldistribution problem in parallel MCHS has been proposed using variable width microchannels. Numerical simulation of copper made parallel MCHS consisting of 25 channels...
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A cryptic ribosome binding site, false signals in reporter systems and avoidance of protein translation chaos
PublicationThe expression of reporter gene may be induced by activation of cryptic signalling sequences, as we found while constructing the mutS-lacZ fusion gene. We cloned the Escherichia coli lacZ gene encoding beta-galactosidase into a plasmid vector carrying the Thermus thermophilus mutS gene. The clones expected to produce beta-galactosidase as the C-terminal fusion were selected for the complementation of beta-galactosidase activity...