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Search results for: RENAL TRANSPLANT, ABU, KLEBSIELLA PNEUMONIAE, URINARY TRACT INFECTION, WHOLE GENOME SEQUENCING, HORIZONTAL GENE TRANSFER
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The New Klebsiella pneumoniae ST152 Variants with Hypermucoviscous Phenotype Isolated from Renal Transplant Recipients with Asymptomatic Bacteriuria-Genetic Characteristics by WGS.
PublicationKlebsiella pneumoniae (Kp) is one of the most important etiological factors of urinary tract infections in renal transplant (RTx) recipients. We described the antimicrobial susceptibility phenotypes and genomic features of two hypermucoviscous (HM) Kp isolates recovered from RTx recipients with asymptomatic bacteriuria (ABU). Using whole genome sequencing (WGS) data, we showed that the strains belong to the ST152 lineage with the...
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Host and pathogen factors in Klebsiella pneumoniae upper urinary tract infections in renal transplant patients
PublicationPurpose . To analyse the role of virulence factors (VFs) and host in Klebsiella pneumoniae upper urinary tract infections (UTIs) in renal transplant (RTx) recipients. Methodology. Clinical and demographic data were registered prospectively. Phylogenetic background of K. pneumoniae isolates was analysed by PCR melting profiles (MP) and the following VFs genes: fimH-1, uge, kpn, ycfM, mrkD, rmpA, magA, hlyA, cnf-1, irp-1, irp-2,...
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Genetic Background and Antibiotic Resistance Profiles of K. pneumoniae NDM-1 Strains Isolated from UTI, ABU, and the GI Tract, from One Hospital in Poland, in Relation to Strains Nationally and Worldwide
PublicationIn recent years, there has been an observed increase in infections caused by carbapenem-resistant Klebsiella pneumonia (Kp) strains. The aim of this study was the phenotypic and genotypic analysis of eight K. pneumoniae NDM (Kp NDM) isolates, recovered in Poland during the years 2016 and 2018 from seven patients with urinary tract infections (UTIs), asymptomatic bacteriuria (ABU), or colonization of the gut. PCR melting profile...
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Urinary Tract Infections Caused by K. pneumoniae in Kidney Transplant Recipients – Epidemiology, Virulence and Antibiotic Resistance
PublicationUrinary tract infections are the most common complication in kidney transplant recipients, possibly resulting in the deterioration of a long-term kidney allograft function and an increased risk of recipient’s death. K. pneumoniae has emerged as one of the most prevalent etiologic agents in the context of recurrent urinary tract infections, especially with multidrug resistant strains. This paper discusses the epidemiology and risk...
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Charakterystyka oporności Klebsiella pneumoniae New Delhi na karbapenemy
PublicationBakterie Klebsiella pneumoniae New Delhi, posiadające gen oporności na karbapenemy (blaNDM) zostały po raz pierwszy wyizolowane w 2008 roku w Szwecji od pacjenta wcześniej hospitalizowanego w Indiach. Bakterie te szybko rozprzestrzeniają się po całym świecie, a od 2011 roku występują również w Polsce. Stanowią one wysokie ryzyko epidemiologiczne, ponieważ gen oporności na karbapenemy przekazywany jest pomiędzy bakteriami poprzez...
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A shear stress micromodel of urinary tract infection by the Escherichia coli producing Dr adhesin
PublicationIn this study, we established a dynamic micromodel of urinary tract infection to analyze the impact of UT-segment-specific urinary outflow on the persistence of E. coli colonization. We found that the adherence of Dr+ E. coli to bladder T24 transitional cells and type IV collagen is maximal at lowest shear stress and is reduced by any increase in flow velocity. The analyzed adherence was effective in the whole spectrum of physiological...
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Whole-genome sequencing and antimicrobial potential of bacteria isolated from Polish honey
PublicationThe aim of this study was the whole-genome analysis and assessment of the antimicrobial potential of bacterial isolates from honey harvested in one geographical location—the north of Poland. In total, 132 strains were derived from three honey samples, and the antimicrobial activity of CFAM (cell-free after-culture medium) was used as a criterion for strain selection and detailed genomic investigation. Two of the tested isolates...
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Optical method supported by machine learning for urinary tract infection detection and urosepsis risk assessment
PublicationThe study presents an optical method supported by machine learning for discriminating urinary tract infections from an infection capable of causing urosepsis. The method comprises spectra of spectroscopy measurement of artificial urine samples with bacteria from solid cultures of clinical E. coli strains. To provide a reliable classification of results assistance of 27 algorithms was tested. We proved that is possible to obtain...
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Whole genome sequencing of Fowl aviadenovirus A - a causative agent of gizzard erosion and ulceration, in adult laying hens
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Virulence analysis and antibiotic resistance of Klebsiella pneumoniae isolates from hospitalised patients in Poland
PublicationKlebsiella pneumoniae (KP) is a nosocomial pathogen causing difficult-to-treat infections. The presence of virulence genes and antibiotic resistance of 109 KP isolates from hospitalized patients were investigated. Among them, 68.8% were multi-drug resistant (MDR) and 59.6% produced extended-spectrum beta-lactamases (ESBLs). Metallo-β-lactamases (MBLs) were produced by 22% of isolates (mainly from anus), including 16.5% of isolates...
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Urinary tract infection in children: Diagnosis, treatment, imaging – Comparison of current guidelines
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Importance of biofilm formation by uropathogenic E. coli Dr+ strains in urinary tract infections
PublicationImportance of biofilm formation by uropathogenic E. coli Dr+ strains in urinary tract infections
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Extended-spectrum beta-lactamase-producing Klebsiella pneumoniae in a neonatal unit: control of an outbreak using a new ADSRRS technique
PublicationSzczepy Klebsiella pneumoniae (EPKP)produkujące beta-laktamazy o rozszerzonym spektrum substratowym (ESBL)stanowią często problem zakażeń na oddziałach noworodkowych. Izolaty od 149 pacjentów poddano typowaniu nową metodą opartą na zjawisku supresji PCR, ADSRRS-fingerprinting, wykazując pochodzenie klonalne badanych szczepów. Użyteczność techniki ADSRRS-finerprinting została potwierdzona innymi metodami - RAPD i PFGE.
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Phage therapy as a novel strategy in the treatment of urinary tract infections caused by E. coli
PublicationUrinary tract infections (UTIs) are regarded as one of the most common bacterial infections affecting millions of people, in all age groups, annually in the world. The major causative agent of complicated and uncomplicated UTIs are uropathogenic E. coli strains (UPECs). Huge problems with infections of this type are their chronicity and periodic recurrences. Other disadvantages that are associated with UTIs are accompanying complications...
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Alternative treatment approaches of urinary tract infections caused by uropathogenic Escherichia coli strains
PublicationUrinary tract infections (UTIs) are the most widespread and annoying infections affecting millions of people every year annually. The biggest problem of urinary diseases are recurrences, the increasing resistance of uropathogens to commonly used antibiotics, as well as the high health care costs of afflicted persons. Uropathogenic Escherichia coli strains (UPECs) are the most dominant etiologic agent of community-acquired infections...
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Asymptomatic Bacteriuria in Kidney Transplant Recipients—A Narrative Review
PublicationUrinary tract infections (UTIs) are the most prevalent complications in kidney transplant (KTx) recipients. The most frequent finding in this group of patients is asymptomatic bacteriuria (ASB). Here, we provide an overview of the available evidence regarding ASB in KTx recipients, including its etiopathology, clinical impact and management. There is a growing body of evidence from clinical trials that screening for and treating...
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Discrimination of hospital isolates of Acinetobacter baumannii using repeated sequences and whole genome alignment differential analysis
PublicationAn optimized method for bacterial strain differentiation, based on combination of Repeated Sequences and Whole Genome Alignment Differential Analysis (RS&WGADA), is presented in this report. In this analysis, 51 Acinetobacter baumannii multidrug-resistance strains from one hospital environment and patients from 14 hospital wards were classified on the basis of polymorphisms of repeated sequences located in CRISPR region, variation...
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Genome-Wide DNA Methylation and Gene Expression in Patients with Indolent Systemic Mastocytosis
PublicationMastocytosis is a clinically heterogenous, usually acquired disease of the mast cells with a survival time that depends on the time of onset. It ranges from skin-limited to systemic disease, including indolent and more aggressive variants. The presence of the oncogenic KIT p. D816V gene somatic mutation is a crucial element in the pathogenesis. However, further epigenetic regulation may also affect the expression of genes that...
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Response to Letter to the Editor re ‘Urinary tract infection in children: Diagnosis, treatment, imaging – Comparison of current guidelines’
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Heat Transfer During Pool Boiling of Water, Methanol and R141b on Porous Coated Horizontal Tube Bundles
PublicationThis paper presents the results of experimental investigation of water, methanol and R141b refrigerant boiling on horizontal porous coated tube bundles supposed to represent a flooded-type evaporator. Experiments were carried out for a bundle of 19 tubes in triangular layout for two pitch-to-diameter ratio values – 1.7 and 2.0 – in atmospheric and sub-atmospheric pressure conditions. Average heat transfer coefficients – both local,...
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Simultaneous determination of creatinine and acetate by capillary electrophoresis with contactless conductivity detector as a feasible approach for urinary tract infection diagnosis
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Evaluation of Chemical Changes in Laboratory-Induced Colistin-Resistant Klebsiella pneumoniae
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Beata Krawczyk dr hab.
PeopleBeata Krawczyk, PhD, Professor Academic degrees, education, qualifications: B. Krawczyk earned his master degree in Biology at the University of Gdansk, Biology Faculty, in 1986, and her PhD in Molecular Biology at the University of Gdansk, Biology Faculty, in 1996. Postdoctoral degree (habilitation) in biological sciences in the discipline biology, Faculty of Natural Sciences, University of Szczecin, Szczecin, in 2009. In 2012...
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LUTS-Lower Urinary Tract Symptoms
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Charakterystyka genetyczna szczepów Klebsiella pneumoniae izolowanych od pacjentów po przeszczepieniu nerki oraz badanie wpływu leków immunosupresyjnych na ekspresję genów bakteryjnych
PublicationBakterie z gatunku Klebsiella pneumoniae należą do oportunistycznych patogenów odpowiedzialnych za zakażenia szpitalne. Drobnoustroje te wykazują skłonność do oporności wielolekowej i wysokiej wirulencji, co stanowi poważne zagrożenie dla pacjentów nieimmunokompetentnych. Wśród pacjentów po transplantacji nerki zakażenia układu moczowego (ZUM) stanowią najczęstszą przyczynę powikłań infekcyjnych. Mechanizmy molekularne leżące u...
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Identification of antigen Ag43 in uropathogenic Escherichia coli Dr+ strains and defining its role in the pathogenesis of urinary tract infections
PublicationUrinary tract infections (UTIs) caused by uropathogenic Escherichia coli (UPEC) are among the most common bacterial infectious diseases in the developed world. The urovirulence of UPEC is mainly associated with the surface-exposed fimbrial adhesins and adhesins of the autotransporter (AT) family. The best studied of theses proteins is antigen 43 (Ag43) mediating cell aggregation, adhesion and biofilm development as the causes of...
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Free Convection Heat Transfer from Horizontal Cylinders
PublicationThe results of experimental investigation of free convection heat transfer in a rectangular container are presented. The ability of the commonly accepted correlation equations to reproduce present experimental data was tested as well. It was assumed that the examined geometry fulfils the requirement of no-interaction between heated cylinder and bounded surfaces. In order to check this assumption recently published correlation equations...
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A new assay for the simultaneous identification and differentiation of Klebsiella oxytoca strains.
PublicationKlebsiella oxytoca is the second most frequently identified species of Klebsiella isolated from hospitalized patients. Klebsiella spp. is difficult to identify using conventional methods and is often misclassified in clinical microbiology laboratories. K. oxytoca is responsible for an increasing number of multi-resistant infections in hospitals because of insufficient detection and identification. In this study, we propose a new...
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Experimental Investigation of Free Convection Heat Transfer from Horizontal Cylinder to Nanofluids
PublicationThe results of free convection heat transfer investigation from a horizontal, uniformly heated tube immersed in a nanofluid are presented. Experiments were performed with five base fluids, i.e., ethylene glycol (EG), distilled water (W) and the mixtures of EG and water with the ratios of 60/40, 50/50, 40/60 by volume, so the Rayleigh (Ra) number range was 3 104 Ra 1.3 106 and the Prandtl (Pr) number varied from 4.4 to 176....
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Urinary Tract Infections of Escherichia coli Strains of Chaperone-Usher System
PublicationZakażenia dróg moczowych stanowią bardzo poważny problem zdrowotny i ekonomiczny dotyczący milionów ludzi, każdego roku na całym świecie. Najbardziej rozpowszechnionym czynnikiem etiologicznym tego typu infekcji bakteryjnych, obejmujących górne i dolne drogi moczowe, są szczepy E. coli, reprezentujące około 80% przypadków. Uropatogenne szczepy E. coli produkują kilka czynników urowirulentncyh, które można podzielić na dwa główne...
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Bacteriophage‐based approach for treatment of urinary tract infections: a quick outlook
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Study of free convective heat transfer from horizontal conic.
PublicationPrzedstawiono teoretyczne rozważania i badania eksperymentalne konwekcji swobodnej od izotermicznej powierzchni poziomego stożka w przestrzeni nieograniczonej. Rozwiązanie przeprowadzono dla wprowadzonego krzywoliniowego układu współrzędnych, opartego na krzywej linii prądu wokół powierzchni stożkowej.
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ArchBGal32cB 441Glu mutein gene analysis dataset
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Prof. Haitham Abu-Rub - A Visit to Poland's Gdansk University of Technology
PublicationReport on visit of Prof. Haitham Abu-Rub in Gdansk University of Technology. Speech on the Smart Grid Centre. Visit in the new smart grid laboratory of the GUT, the Laboratory for Innovative Power Technologies and Integration of Renewable Energy Sources (LINTE^2).
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Enterococci from ready-to-eat food - horizontal gene transfer of antibiotic resistance genes and genotypic characterization by PCR melting profile
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Identification of Antigen 43 in uropathogenic Escherichia coli IH11128 responsible for persistence in the urinary tract
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Statistical-based approach in potential diagnostic application of urinary nucleosides in urogenital tract cancer
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Nonradiative long range energy transfer in donor-acceptor systems with excluded volume
PublicationW pracy analizowano bezpromienisty długozasięgowy transfer energii w układach donor-akceptor z uwzględnieniem objętości wyłączonej. Stwierdzono, że zanik fluorescencji pierwotnie wzbudzonych donorów jest wolniejszy, jeżeli uwzględnia się objętość wyłączoną. Ten efekt jest znacznie wzmocniony, gdy akceptory znajdują się w pewnej objętości niedostępnej dla donorów. Analizę numeryczną przeprowadzono stosując metodę symulacji Monte...
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Results from a Genome-Wide Association Study (GWAS) in Mastocytosis Reveal New Gene Polymorphisms Associated with WHO Subgroups
PublicationMastocytosis is rare disease in which genetic predisposition is not fully understood. The aim of this study was to analyze associations between mastocytosis and single nucleotide polymorphisms (SNPs) by a genome-wide association study (GWAS) approach. A total of 234 patients were enrolled in our study, including 141 with cutaneous mastocytosis (CM; 78 children and 63 adults) and 93 with systemic mastocytosis (SM, all adults)....
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Adiponectin is unrelated to kidney function or injury markers in renal transplant recipients: A one-year follow-up study
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Influence of nanoparticle concentration on convective heat transfer of water-Al2O3 nanofluids inside horizontal tubes
PublicationNanofluids came to be seen as a new generation of coolants, especially in single-phase systems. Conducted experiments indicate that the increase in thermal conductivity is not the only reason for the intensification of heat transfer when using nanofluids. Preliminary results of the experimental investigation of the forced convection heat transfer of water-Al2O3 nanofluids inside stainless steel tube with 8 mm internal diameter...
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The safety issue of roadside advertising – comparison of polish and abu dhabi regulations
PublicationIn Poland a large number of advertisements are located by the roadside. These ads do not support road traffic management and unlike the road marking system are not subject to any regulations. The advertiser’s goal is to communicate a message to as many recipients as possible. Drivers with different individual abilities, such as attention focusing, eye accommodation, speed of information processing, can be distracted, blinded or...
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TRNA-Leu (UUR) gene haplotypes observed in canine mammary gland tumours and its deleterious effect assessment according to the comparative analysis with TRNL1 human gene
Open Research DataThe aetiology and pathogenesis of many canine tumours are likely to be similar to cancers found in humans. This study aimed to present a plausible link between changes in tRNA-Leu (UUR) gene and the carcinogenesis process in dogs with mammary gland tumours. The whole mitochondrial DNA (mtDNA) isolated from blood and tumour tissues of 13 dogs with malig-nant...
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Influence of nanoparticle concentration on convective heat transfer of water-Al2O3 nanofluids inside horizontal tubes
PublicationThis work presents preliminary results of the experimental investigation of the forced convection heat transfer of water-Al2O3 nanofluids inside stainless steel tube with 8 mm internal diameter and 2000 mm length. Nanoparticles were tested at the concentrations of 0.01%, 0.1%, 1% and 5% by weight and the Reynolds number range encompasses laminar as well as turbulent flows.
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Parasites of the digestive tract of sheep and goats from organic farms in Western Pomerania, Poland
PublicationThe aim of the study was to determine the prevalence and intensity of parasite infection of the digestive tract in sheep and goats from the West Pomerania region following anti-parasite treatment. Feces were freely collected from sheep and goats kept on organic farms and subjected to analysis by the Willis-Schlaf and McMaster’s flotation methods. The mean extensity of infection by gastrointestinal parasites...
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Phenotypic consequences of the LYS4 gene disruption in Candida albicans
PublicationThe main scientific purpose of our studies was to verify the hypothesis that homoaconitase (HA) from Candida albicans, an enzyme catalyzing a second step of the α-aminoadipate pathway (AAP) of L-Lys biosynthesis may become a new target for antifungal chemotherapy. Previous studies indicated that the A. fumigatus mutant lacking the functional lysF gene, encoding HA, exhibited attenuated virulence in a low-dose mouse infection model...
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Experimental study of natural convection heat transfer from horizontal conic
PublicationPrzedstawiono badania eksperymentalne i ich wyniki konwekcji swobodnej w wodzie i powietrzu poziomych stożków o kącie przy podstawie 30, 45 i 60 deg. Wyniki badań wykazują dobrą zgodność z rozwiązaniem teoretycznym i z danymi literaturowymi.
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An examination of an Antarctic soil metagenomic-derivate putative methylthioadenosine phosphorylase gene as a novel reporter gene for promoter trapping
PublicationThe rsfp gene was discovered during examination ofan Antarctic soil-derived metagenome library and encodesa bacterial methylthioadenosine phosphorylase (GenBank accession number GQ202582). In a previous study, we found that E. coli colonies expressing the rsfp gene reveal strong pink fluorescence after exposure to UV light when grown on culture medium supplemented with rhodamine B (Cieśliński et al., 2009). In contrast to that,...
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A Comparability of Renal Length and Volume Measurements in MRI and Ultrasound in Children
PublicationIntroduction: Despite the significant increase in use of magnetic resonance imaging (MRI) in children, there is still a lack of normal reference values of renal size in this method and reference values are being interpolated from the ultrasound (US) studies. The study provides comparative analysis of agreement in renal length and volume measurements between MRI and ultrasound. Materials and Methods: Ninety-three children with a...
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Tetracycline and ampicillin resistance of Escherichia coli strains of surface water origin: the potential for horizontal transfer of resistance genes = Oporność na tetracyklinę i ampicylinę szczepów Escherichia Coli wyizolowanych z wody powierzchniowej : możliwość horyzontalnego przenoszenia genów oporności
PublicationThe aim of this preliminary study was to assess the occurrence and molecular diversity of tetracycline and ampicillin resistance genes carried by Escherichia coli present in surface water. Bacterial strains were isolated from two watercourses (the Reda River and the Oliwski Stream) that influence the quality of coastal waters in the Gdańsk Bay (Northern Poland) by direct discharge. The bacterial drug susceptibility, tested against...