Filters
total: 4306
filtered: 3708
-
Catalog
- Publications 3708 available results
- Journals 39 available results
- Conferences 10 available results
- Publishing Houses 1 available results
- People 62 available results
- Projects 3 available results
- Laboratories 2 available results
- e-Learning Courses 41 available results
- Events 5 available results
- Open Research Data 435 available results
Chosen catalog filters
displaying 1000 best results Help
Search results for: PROTEIN FOLDING
-
Recent Developments in Data-Assisted Modeling of Flexible Proteins
PublicationMany proteins can fold into well-defined conformations. However, intrinsically-disordered proteins (IDPs) do not possess a defined structure. Moreover, folded multi-domain proteins often digress into alternative conformations. Collectively, the conformational dynamics enables these proteins to fulfill specific functions. Thus, most experimental observables are averaged over the conformations that constitute an ensemble. In this...
-
Dynamics of Chains as a Tool to Study Thermomechanical Properties of Proteins.
PublicationPolymer dynamics can be formulated on different levels of detail. One approach eliminates microscopic degrees of freedom and a polymer molecule is represented by a simplified structure—a chain. In the simplest case monomers of ideal chain have fixed length, and their orientation is independent of the orientations and positions of neighbouring monomers. This is reason that two monomers can co-exist at the same place. Ideal chain...
-
Mechanism of Osmolyte Stabilization–Destabilization of Proteins: Experimental Evidence
PublicationIn this work, we investigated the influence of stabilizing (N,N,N-trimethylglycine) and destabilizing (urea) osmolytes on the hydration spheres of biomacromolecules in folded forms (trpzip-1 peptide and hen egg white lysozyme─hewl) and unfolded protein models (glycine─GLY and N-methylglycine─NMG) by means of infrared spectroscopy. GLY and NMG were clearly limited as minimal models for unfolded proteins and should be treated with...
-
The construction of bifunctional fusion proteins consisting of MutS and GFP
PublicationSkonstruowano białka chimeryczne zawierające białko MutS z Thermus thermophilus i białko zielonej fluoresceiny GFP z Aequorea victoria, posiadające dla łatwiejszego oczyszczania domeny oligo-histydynowe na N- lub C-końcu. Fuzyjne białka rozpoznawały niekomplementarności w DNA podobnie do MutS T. thermophilus. Fluorescencyjne białka rozpoznające niekomplementarne DNA mogą być użyteczne w wykrywaniu jednonukleotydowych polimorfizmów...
-
Fading modelling in dynamic off-body channels
Publication -
Tensor-product versus geometric-product coding
PublicationKodowanie przy pomocy iloczynów tensorowych, a kodowanie przy pomocy iloczynów geometrycznych. Formalizm jest zilustrowany przy pomocy paru przykładów.
-
Transient detection algorithms for speech coding applications
Publication -
Fading Modelling in Dynamic Off-Body Channels
PublicationThis paper presents an off-body fading channel model for Body Area Networks (BANs) in indoor environments. The proposed model, which is based on both simulations and measurements in a realistic environment, consists of three components: mean path loss, body shadowing, and multipath fading. Seven scenarios in an indoor environment (a medium-size room with furniture, mostly consisting of wooden tables and chairs) have been measured:...
-
Fading Modeling in Maritime Container Terminal Environments
PublicationIn this paper, an analytical model for slow and fast fading effects in maritime container terminals is derived, from fitting distributions to the results of measurements performed in an actual operational environment. The proposed model is composed of a set of equations, enabling to evaluate fading statistical distribution parameters for different system and environments conditions, as a function of frequency, base station antenna...
-
Fading Modelling in Dynamic Off-Body Channels
PublicationThis paper presents an off-body fading channel model for Body Area Networks. The proposed model, based on both simulations and measurements at 2.45 GHz in a realistic indoor environment, consists of three components: mean path loss, body shadowing, and multipath fading. The first is modelled as a log function of distance, the path loss exponent being in between 0.4 and 1.6. A statistical perspective is taken for the other two components,...
-
Proton affinity and proton transfer energy for selected organic molecules
PublicationThe Hartree-Fock method in 6-311G** molecular orbitals basis set has been applied to calculations of proton affinities and proton transfer reaction energies for water, methanol, acetaldehyde, ethanol, acetone, acetic acid, methyl acetate and ethyl acetate.
-
Effect of ultrasound treatment on water holding properties and microstructure of beef (m. semimembranosus) during ageing
Publication -
Novel thermostable single-stranded DNA-binding protein (SSB) from Deinococcus geothermalis
PublicationW celu zbadania właściwości biochemicznych białka wiążącego się do jednoniciowego DNA (SSB) pochodzącego z Deinococcus geothermalis (DgeSSB), sklonowano gen ssb otrzymany w reakcji PCR, a następnie uzyskano wydajną biosyntezę białka DgeSSB. Gen składa się z 900 par zasad, kodujących białko złożone z 300 reszt aminokwasowych, o wyliczonej masie molekularnej równej 32.45 kDa. Sekwencja aminokwasowa wykazuje 43, 44% i 75% identyczności...
-
Expression profiles and genomic organisation of group A protein phosphatase 2C genes inBrassica oleracea
Publication -
An analysis and evaluation of the WeFold collaborative for protein structure prediction and its pipelines in CASP11 and CASP12
Publication -
Adipocyte Fatty Acid‐Binding Protein as a Novel Marker of Psoriasis and Clinical Response to Acitretin
Publication -
Novel Drying Methods for Sustainable Upcycling of Brewers’ Spent Grains as a Plant Protein Source
Publication -
The effect of topical treatment and psoriasis severity on serum retinol-binding protein-4 levels
Publication -
A Data Driven Model for Predicting RNA-Protein Interactions based on Gradient Boosting Machine
Publication -
Patients with obstructive sleep apnea present with chronic upregulation of serum HIF-1α protein
Publication -
Characterization of Structural and Energetic Differences between Conformations of the SARS-CoV-2 Spike Protein
Publication -
Comparison of protein precipitation methods for various rat brain structures prior to proteomic analysis
Publication -
Similarities and Differences in the Protein Composition of Cutaneous Melanoma Cells and Their Exosomes Identified by Mass Spectrometry
Publication -
The plasma concentration of advanced oxidation protein products and arterial stiffness in apparently healthy adults
Publication -
Blood plasma protein and lipid profile changes in calves during the first week of life
Publication -
Novel single-stranded DNA-binding protein from psyvhrophilic bacterium Psychrobacter arcticus
PublicationTo study the biochemical properties of SSB from Psychrobacter arcticus (ParSSB), we have cloned the ssb genes obtained by PCR and have developed Escherichia coli overexpression systems. The gene consists of an open reading frame of 642 nucleotides encoding SSB protein of 213 amino acids with a calculated molecular mass of 22.8 kDa. The amino-acid sequence of ParSSB exhibits 49% identity and 57% similarity to Escherichia coli SSB....
-
MIC1-MAG1-SAG1 chimeric protein, a most effective antigen for detection of human toxoplasmosis
PublicationThis study describes a Toxoplasma gondii IgG ELISA test based on a new chimeric antigen containing three immunodominant regions from MIC1, MAG1 and SAG1 proteins of a parasite and shows that this test is useful for diagnostic purposes and may replace the lysed and whole-cell antigens.
-
Unusual dynamic properties of water near the ice-binding plane of hyperactive antifreeze protein
PublicationThe dynamical properties of solvation water of hyperactive antifreeze protein from Choristoneura fumiferana (CfAFP) are analyzed and discussed in context of its antifreeze activity. The protein comprises of three well-defined planes and one of them binds to the surface of ice. The dynamical properties of solvation water around each of these planes were analyzed separately; the results are compared with the dynamical properties...
-
A highly thermostable, homodimeric single-stranded DNA-binding protein from Deinococcus radiopugnans
PublicationWe report the identification and characterization of the single-stranded DNA-binding protein (SSB) from the mesophile and highly radiation-resistant Deinococcus radiopugnans (DrpSSB). PCR-derived DNA fragment containing the complete structural gene for DrpSSB protein was cloned and expressed in Escherichia coli. The gene consisting of an open reading frame of 900 nucleotides encodes a protein of 300 amino acids with a calculated...
-
Aqueous solutions of NMA, Na2HPO4 , and NaH2PO4 as models for interaction studies in phosphate–protein systems
PublicationPhosphate buffers are essential for many areas of studies. However, their influence on buffered systems is often ignored. The phosphate salts can interact with biologically important macromolecules (e.g. proteins) and stabilize or destabilize them. With our research, we want to answer question what kind of interactions, if any, occur between phosphate ions and a protein backbone model — N-methylacetamide (NMA). ATR-FTIR spectroscopy...
-
Expression of goose parvovirus whole VP3 protein and its epitopes in Escherichia coli cells
PublicationThe aim of this study was the expression of goose parvovirus capsid protein (VP3) and its epitopes in Escherichia coli cells. Expression of the whole VP3 protein provided an insufficient amount of protein. In contrast, the expression of two VP3 epitopes (VP3ep4, VP3ep6) in E. coli, resulted in very high expression levels. This may suggest that smaller parts of the GPV antigenic determinants are more efficiently expressed than the...
-
Integration of protein tethering in a rapid and label-free SERS screening platform for drugs of abuse
PublicationSurface enhanced Raman spectroscopy (SERS) has emerged as a promising technique for the rapid and ultrasensitive detection of molecular species such as drugs of abuse in biofluids. Yet, it remains a significant challenge to create a viable screening tool for multiple drug classes, owing to the lack of affinity of certain species for the SERS substrate and to the matrix interference in complex media. Here we report a protein tethering...
-
Proteolysis of whey protein isolates in nanoemulsion systems: impact of nanoemulsification and additional synthetic emulsifiers
PublicationNanoemulsions are currently of interest in the functional food sector because their small droplet size (100–500 nm) provides a number of potential advantages over conventional emulsions. This study concerned the behavior of nanoemulsions stabilized with whey proteins and two synthetic emulsifiers (Tween 80 and Croduret), and exposed to conditions simulating the human upper gastrointestinal tract. In particular, the effect of synthetic...
-
The Role of Electrostatics in Enzymes: Do Biomolecular Force Fields Reflect Protein Electric Fields?
PublicationPreorganization of large, directionally oriented, electric fields inside protein active sites has been proposed as a crucial contributor to catalytic mechanism in many enzymes, and it may be efficiently investigated at the atomistic level with molecular dynamics simulations. Here, we evaluate the ability of the AMOEBA polarizable force field, as well as the additive Amber ff14SB and Charmm C36m models, to describe the electric...
-
A Data Driven Model for Predicting RNA-Protein Interactions based on Gradient Boosting Machine
PublicationRNA protein interactions (RPI) play a pivotal role in the regulation of various biological processes. Experimental validation of RPI has been time-consuming, paving the way for computational prediction methods. The major limiting factor of these methods has been the accuracy and confidence of the predictions, and our in-house experiments show that they fail to accurately predict RPI involving short RNA sequences such as TERRA RNA....
-
ESCASA : Analytical estimation of atomic coordinates from coarse‐grained geometry for nuclear‐magnetic‐resonance ‐assisted protein structure modeling. I. Backbone and Hβ protons
PublicationA method for the estimation of coordinates of atoms in proteins from coarse-grained geometry by simple analytical formulas (ESCASA), for use in nuclear-magnetic-resonance (NMR) data-assisted coarse-grained simulations of proteins is proposed. In this paper, the formulas for the backbone Hα and amide (HN) protons, and the side-chain Hβ protons, given the Cα-trace, have been derived and parameterized, by using the interproton distances...
-
Artificial neural network based fatigue life assessment of friction stir welding AA2024-T351 aluminum alloy and multi-objective optimization of welding parameters
PublicationIn this paper, the fracture behavior and fatigue crack growth rate of the 2024-T351 aluminum alloy has been investigated. At first, the 2024-T351 aluminum alloys have been welded using friction stir welding procedure and the fracture toughness and fatigue crack growth rate of the CT specimens have been studied experimentally based on ASTM standards. After that, in order to predict fatigue crack growth rate and fracture toughness,...
-
Resistance of Polypropylene Membrane to Oil Fouling during Membrane Distillation
Publication -
Fouling of nanofiltration membranes used for separation of fermented glycerol solutions
Publication -
Long-Term Performance of Ultrafiltration Membranes: Corrosion Fouling Aspect
Publication -
Numerical Simulations of Laser and Hybrid S700MC T-Joint Welding
Publication -
Computational Techniques in Numerical Simulations of Arc and Laser Welding Processes
Publication -
APPLICATION OF NUMERICAL SIMULATIONS ON X10CRWMOVNB9-2 STEEL MULTILAYER WELDING
Publication -
Bonding of doxorubicin to nanosilica and human serum albumin in various media
Publication -
Destructive Consequences of PWM Inverter Feeding for Traction AC Motors
PublicationThe electric traction drives with an AC motor fed by a frequency converter are used more and more often nowadays. The most significant side effects, of a converter's output voltage forming PWM method for AC motor windings, are steep voltage changes, which can initiate several destructive phenomena. The paper presents the adverse influence of a converter common mode voltage on the motor windings' increased voltage exposure and the...
-
The rolling contact fatigue of WC/C-coated spur gears
Publication -
Vibration Diagnostics of Rolling Mills Based on Nonlinear Effects in Dynamics
Publication -
NMR and MD Analysis of the Bonding Interaction of Vancomycin with Muramyl Pentapeptide
Publication -
Enzymatic Activity as New Moorsh-Forming Process Indicators of Peatlands
Publication -
Reproducibility of Finding Enriched Gene Sets in Biological Data Analysis
Publication