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Search results for: ENZYMATIC CONVERSIONS
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Enzymatic activities behind degradation of glucosinolates
PublicationMyrosinase (thioglucosidase, EC 3.2.1.147) is the enzyme responsible for the hydrolysis of glucosinolates (GLs). In plant tissue, myrosinase and GLs are sequestered in separate cellular compartments. As a result of cell disruption, e.g., after pathogen attack or on chopping or grinding during food preparation, the myrosinase comes into contact with GLs and catalyzes the hydrolysis of thioglucosidic bond in GL structure. Consequently,...
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Enantioselective enzymatic desymmetrization of the prochiral pyrimidine acyclonucleoside
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Production of trehalose in a single step enzymatic reaction
PublicationThe aim of this study was isolation and cloning of trehalose synthase gene derived from extremophilic microorganisms Deinoccoci to the expressive E. coli vectors and its biosythesis in different hosts.
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Performance of ASON/GMPLS architecture in condition of wavelength conversion and without wavelength conversion
PublicationThe paper regards the problem of ASON/GMPLS performance in condition of wavelength and without wavelength conversion. The authors present influence analysis in condition of wavelength and non-wavelength conversion on the ASON/GMPLS control plane performance represented by mean Connection Set-up Time E (CST) and request loss probabilities. The offered traffic to the transport layer and request intensity have been changed for evaluation...
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Photocatalysts for Solar Energy Conversion
PublicationPhotocatalytic oxidation over suspended solid photocatalysts (e.g., titanium dioxide) has been proposed as a sustainable process for treatment and purification of water and wastewater as well as the process for photoconversion of solar energy to hydrogen, methane, and other low hydrocarbons. Solar radiation could be used to drive heterogeneous electrochemical reactions at the surface of an optically active photocatalyst (e.g.,...
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Thermal Biomass Conversion: A Review
PublicationIn this paper, the most important methods of thermal conversion of biomass, such as: hydrothermal carbonization (180–250 °C), torrefaction (200–300 °C), slow pyrolysis (carbonization) (300–450 °C), fast pyrolysis (500–800 °C), gasification (800–1000 °C), supercritical steam gasification, high temperature steam gasification (>1000 °C) and combustion, were gathered, compared and ranked according to increasing temperature. A comprehensive...
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Modeling organic nitrogen conversions in activated sludge bioreactors
PublicationFor biological nutrient removal (BNR) systems designed to maximize nitrogen removal, the effluent total nitrogen (TN) concentration may range from 2.0 to 4.0 g N/m3 with about 25-50% in the form of organic nitrogen (ON). In this study, current approaches to modeling organic N conversions (separate processes vs. constant contents of organic fractions) were compared. A new conceptual model of ON conversions was developed and combined...
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Modeling organic nitrogen conversions in activated sludge bioreactors
PublicationOpracowano model przemian azotu organicznego (Norg)jako rozszerzenie Modelu Osadu Czynnego Nr 2d (ASM2d). Nowy model zawiera trzy frakcje Norg: rozpuszczoą, koloidalną i zawesinową. Każda frakcja została podzielona na dwie podfrakcje - rozkładalną i nierozkładalną. Model został zweryfikowany w oparciu o wyniki badań eksperymentalnych w oczyszczalni ścieków Gdańsk-Wschód.
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Enzymatic Activity as New Moorsh-Forming Process Indicators of Peatlands
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Sandwich Biobattery with Enzymatic Cathode and Zinc Anode Integrated with Sensor
PublicationCarbon paper covered with side-naphthylated multi walled carbon nanotubes was used as the conducting support for the construction of a biocathode in a hybrid biofuel cell. Laccase Carrena unicolor enzyme was employed as the catalyst for the 4e reduction of oxygen and a zinc disc covered with hopeite was used as the anode. Derivatized carbon nanotubes increase the working surface of the electrode and provide direct contact with...
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Conversion of a diesel engine bus into a trolleybus
PublicationPrzedsiębiorstwo Komunikacji Trolejbusowej w Gdyni od 2004 roku we własnym zakresie buduje trolejbusy w oparciu o nadwozia używanych autobusów. W monografii przedstawiono przyczyny rozpoczęcia tego przedsięwzięcie, jego przebieg oraz techniczny budowy trolejbusu w oparciu o nadwozie autobusowe.
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Nanomaterials for Environmental Purification and Energy Conversion
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Electrochemical simulation of enzymatic transformations studied for the selected antitumor acridine derivatives
PublicationThe elucidation of the metabolic pathways and the biotransformation mechanisms of potential drugs is a crucial point in drug development. It allows to know the activation routes of the new biologically active compounds, especially in respect to their possible toxicity. Generally, in vivo or in vitro experiments with liver microsomes or hepatocytes are performed. However, these testing schemes are tedious, time consuming and of...
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Green chemical and hybrid enzymatic pretreatments for lignocellulosic biorefineries: Mechanism and challenges
PublicationThe greener chemical and enzymatic pretreatments for lignocellulosic biomasses are portraying a crucial role owing to their recalcitrant nature. Traditional pretreatments lead to partial degradation of lignin and hemicellulose moieties from the pretreated biomass. But it still restricts the enzyme accessibility for the digestibility towards the celluloses and the interaction of lignin-enzymes, nonproductively. Moreover, incursion...
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Enzymatic electrodes nanostructured with functionalized carbon nanotubes for biofuel cell applications
PublicationNanostructured bioelectrodes were designed and assembled into a biofuel cell with no separating membrane.
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The role of the enzymatic antioxidant system in cylindrospermopsin-induced toxicity in human lymphocytes
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Studies on the application of the Passerini reaction and enzymatic procedures to the synthesis of tripeptide mimetics
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Rat cathepsin K: Enzymatic specificity and regulation of its collagenolytic activity
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Effect of pesticides on microorganisms, enzymatic activity and plant in biochar-amended soil
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Enzymatic degradation and transport of endothiopeptides into Escherichia coli K12 mutant strains
PublicationZbadano transport trzech otrzymanych na drodze syntezy ditiopeptydów do komórek Escherichia coli K12. Ponadto zbadano szybkość hydrolizy tych związków. Wszystkie peptydy są transportowane przez permeazy, natomiast są bardziej oporne na hydrolizę enzymatyczną w stosunku do naturalnych peptydów.
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Psychogenic Belching – a rare manifestation of conversion disorder
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Psychogenic Belching – a rare manifestation of conversion disorder
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Sample Rate Conversion with Fluctuating Resampling Ratio
PublicationIn this paper a sample rate conversion with continuously changing resampling ratio has been presented. The proposed implementation is based on variable fractional delay filter implemented using a Farrow structure. It have been demonstrated that using the proposed approach instantaneous resampling ratio can be freely changed. This allows for simulation of audio recored on magnetic tape with nonuniform velocity as well as removal...
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Sample Rate Conversion with Fluctuating Resampling Ratio
PublicationIn this paper a sample rate conversion with continuouslychanging resampling ratio has been presented. The proposed implementation is based on variable fractional delay filter implemented using a Farrow structure. It have been demonstrated that using the proposed approach instantaneous resampling ratio can be freely changed. This allows for simulation of audio recored on magnetic tape with nonuniform velocity as well as removal...
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Automatic conversion of legacy applications into services in beesycluster
PublicationPrzedstawiono rozwiązanie, dzieki któremu uzytkownikw prosty sposób uzyskuje mozliwość automatycznej konwersji aplikacji dostępnych w systemach Unix do usług w systemie BeesyCluster. system BeesyCluster stanowi warstę pośredniczącą w dostepie do sieci klasrów poprzez WWW. Aby zapewnić szeroki zakres dostępnych usług mozliwa jest konwersja wielu pakietów linuksowych jednoczesnie. narzędzie umozliwia na podstawie wydobytych informacji...
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Nitrobenzimidazoles as Substrates for DT-Diaphorase and Redox Cycling Compounds: Their Enzymatic Reactions and Cytotoxicity
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Molecular mechanism of the enzymatic oxidation investigated for imidazoacridinone antitumor drug C-1311.
PublicationPraca miala na celu określenie molekularnego mechanizmu enzymatycznej utleniającej aktywacji wymienionego związku przeciwnowotworowego w takim układzie modelowym, w którym wcześniej wykazano jego kowalencyjne wiązanie się do DNA. Badania struktur chemicznych produktów enzymatycznej aktywacji wykazały, że dwa z nich powstają w wyniku dealkilacji łańcucha bocznego cząsteczki, natomiast dwa inne w wyniku aktywacji pierścienia imidazoakrydonu....
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Membrane Filtration-Assisted Enzymatic Hydrolysis Affects the Biological Activity of Potato Juice
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A Redundant Role of Human Thyroid Peroxidase Propeptide for Cellular, Enzymatic, and Immunological Activity
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The propeptide of human thyroid peroxidase is not required for its cellular, enzymatic or immunological activity
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Materials for Mediator-free Electron Transfer in the Enzymatic Electrodes of Biobatteries and Biofuel Cells
PublicationWe present functionalization of single-walled carbon nanotubes (SWCNTs) and their application in the enzymatic fuel cells construction. SWCNTs were covalently modified with different types of aromatic moieties. Free radical reaction was employed to create direct carbon-carbon bond between moiety and SWCNTs. In second approach functionalization takes place through amide bonding. Functionalized SWCNTs were characterized by spectroscopy...
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Heteropolyanion-doped polypyrrole as catalyst for ethyl alcohol conversion
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H3PMo12O40-doped polyacetylene as a catalyst for ethyl alcohol conversion
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Conversion of Potato Industry Waste into Fodder Yeast Biomass
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Fractional Delay Filter Design for Sample Rate Conversion
PublicationRosnąca liczba różnych standardów szybkości próbkowania stwarza potrzebę stosowania algorytmów konwersji szybkości próbkowania ciągów dyskretnych. Jeżeli współczynnik przepróbkowania nie jest wyrażony jako stosunek małych liczb całkowitych albo gdy nie jest on stały, to rozwiązaniem może być użycie filtrów ułamkowoopóźniających. Umożliwiają one realizację przepróbkowania z dowolną krotnością. Dokładność przepróbkowania algorytmów...
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Early Stages of RNA-Mediated Conversion of Human Prions
PublicationPrion diseases are characterized by the conversion of prion proteins from a PrPC fold into a disease-causing PrPSC form that is self-replicating. A possible agent to trigger this conversion is polyadenosine RNA, but both mechanism and pathways of the conversion are poorly understood. Using coarse-grained molecular dynamic simulations we study the time evolution of PrPC over 600 μs. We find that both the D178N mutation and interacting...
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Hydrogen activated axial inter-conversion in SiC nanowires
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Modelling, thermography and digital processing of energy conversion processes
PublicationW pracy przedstawiono zagadnienia modelowania fizycznego i numerycznego procesów wymiany ciepła i konwersji energii. Do analizy wymiany ciepła w przestrzeni wypełnionej płynem zastosowano termografie krystaliczne i komputerowy system analizy otrzymanych laserowych obrazów. Wykonano również termogramy powierzchni pokrytych także ciekłokrystaliczniei jako przykład przeanalizowano rozkład temperatury na powierzchniach ciał stałych...
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Expression of Deinococcus geothermalis Trehalose Synthase Gene in Escherichia coli and Its Enzymatic Properties
PublicationA novel trehalose synthase gene from Deinococcus geothermalis (DSMZ 11300) containing 1,692 bp reading-frame encoding 564 amino acids was amplified using PCR. The gene was ligated into pET30Ek/LIC vector and expressed after isopropyl alfa-D-thiogalactopyranoside induction in Escherichia coli BL21(DE3)pLysS. The recombinant trehalose synthase (DgeoTreS) containing a His6 tag at the C-terminus was purified by metal affinity chromatography...
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Characterization of Aliphatic Polyesters Synthesized via Enzymatic Ring-Opening Polymerization in Ionic Liquids
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Non-enzymatic flexible glucose sensing platform based on nanostructured TiO2–Au composite
PublicationAll over the world the number of people suffering from diabetes and related complications is drastically growing. Therefore, the need for accurate, reliable and stable sensor for monitoring of glucose in human body fluids is becoming highly desirable. In this work we show that material composed of gold layers deposited onto TiO2 nanotubes (NTs) formed onto the flexible Ti foil exhibits great response toward glucose oxidation and...
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Hydrogen Production from Energy Poplar Preceded by MEA Pre-Treatment and Enzymatic Hydrolysis
PublicationThe need to pre-treat lignocellulosic biomass prior to dark fermentation results primarily from the composition of lignocellulose because lignin hinders the processing of hard wood towards useful products. Hence, in this work a two-step approach for the pre-treatment of energy poplar, including alkaline pre-treatment and enzymatic saccharification followed by fermentation has been studied. Monoethanolamine (MEA) was used as the...
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Enzymatic cross-linking of β-lactoglobulin in solution and at air–water interface: Structural constraints
PublicationEffective and controlled use of cross-linking enzymes in structure engineering of food systems depends on characterization of the favorable conditions for enzyme-substrate complex and the limiting factors for the desired modification. In this respect, we analyzed the susceptibility of bovine β-lactoglobulin (BLG) to enzymatic cross-linking by Trichoderma reesei tyrosinase (TrTyr) and transglutaminase (TG). Changes in BLG molecular...
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Optimization of Saccharification Conditions of Lignocellulosic Biomass under Alkaline Pre-Treatment and Enzymatic Hydrolysis
PublicationPre-treatment is a significant step in the production of second-generation biofuels from waste lignocellulosic materials. Obtaining biofuels as a result of fermentation processes requires appropriate pre-treatment conditions ensuring the highest possible degree of saccharification of the feed material. An influence of the following process parameters were investigated for alkaline pre-treatment of Salix viminalis L.: catalyst concentration...
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Enzymatic and Chemical Cross-Linking of Bacterial Cellulose/Fish Collagen Composites—A Comparative Study
PublicationThis article compares the properties of bacterial cellulose/fish collagen composites (BC/Col) after enzymatic and chemical cross-linking. In our methodology, two transglutaminases are used for enzymatic cross-linking—one recommended for the meat and the other proposed for the fish industry—and pre-oxidated BC (oxBC) is used for chemical cross-linking. The structure of the obtained composites is characterized by scanning electron...
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Offshore project management based on jack-up rig conversion
PublicationChapter presents aspects of Offshore project management during conversion drilling rig to accommodation rig in Batam Indonesia
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Annual variations in the solar energy conversion efficiency in will coppice stand.
PublicationMonitorowano produkcyjność plantacji wiklinowych w Uppsali w Szwecji w latach 1985-1994. Okazało się, że największa sprawność przetwarzania energii słonecznej w biomasę ma miejsce w drugim i trzecim roku uprawy wiklinowej (około 1%), natomiast w pierwszym i w czwartym roku sprawność ta jest 2 razy niższa i wynosi około 0,5%. Dyskutuje się przyczyny takiej sytuacji.
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Polarization Conversion of Solitons Propagating in Birefringent Microstructured Optical Fibers
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On Sample Rate Conversion Based on Variable Fractional Delay Filters
PublicationThe sample rate conversion algorithm based on variable fractional delay filters is often used if the resampling ratio cannot be expressed as the ratio of small integer numbers or if it is not constant. The main advantage of such solution is that it allows for arbitrary resampling ratios which can even be changed during the resampling process. In this paper a discussion on influence of different approaches to fractional filter...
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Sample Rate Conversion Based on Frequency Response Masking Filter
PublicationThe sample rate conversion with high resampling ratios requires low-pass digital filters with very narrow transition band which results in high computational complexity and makes filter design problematic. Therefore in this work we propose to use the FRM method, which breaks the filter with a narrow transition band into a group of filters with reduced design requirements. These decreases the number of non-zero coefficients and...