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Search results for: REAL-TIME PCR
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Short-term Cultivation of Porcine Cumulus Cells Influences the Cyclin-dependent Kinase 4 (Cdk4) and Connexin 43 (Cx43) Protein Expression—A Real-time Cell Proliferation Approach
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Novel analytical approach for real-time monitoring of volatile Maillard reaction products emitted from the sugar-amino acid model system using proton transfer reaction mass spectrometry
PublicationIn the presented research, volatile Maillard reaction products formation in the two sugar-amino acid model systems, namely glucoselysine and ribose-lysine model systems were investigated using proton transfer reaction mass spectrometry. Obtained data were supported by the reference method, i.e., UV/Vis spectrometry. A number of volatile organic compounds were selected based on the correlation of the effect of Maillard reaction...
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Euromicro Conference on Real-Time Systems
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Active and Real-Time Database Systems
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Ontology groups representing angiogenesis and blood vessels development are highly up-regulated during porcine oviductal epithelial cells long-term real-time proliferation – a primary cell culture approach
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International Conference on Real-Time and Network Systems
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Australasian Conference on Parallel and Real-Time Systems
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Formal Techniques in Real-Time and Fault Tolerant Systems
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IEEE Real-Time and Embedded Technology and Applications Symposium
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International Symposium on Distributed Simulation and Real Time Applications
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International Conference on Embedded and Real Time Computing Systems and Applications (International Conference on Real-Time and Embedded Computing Systems and Applications)
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IEEE International Symposium on Real-Time Distributed Computing (IEEE International Symposium on Object-Oriented Real-Time Distributed Computing)
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Real-Time Operating Microsystems 2023/2024
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IEEE International Workshop on Distributed Intelligent Simululation and Real-Time Applications
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IEEE International Workshop on Object-Oriented Real-Time Dependable Systems
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Real-time Operating Systems - Seminar 2023/4
e-Learning CoursesPage to support seminar clases of Real-time Operating Systems
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Real Time Operating Systems - Seminar 2021/22L
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Real time operating systems Seminars 2020-2021
e-Learning CoursesRTOS seminary classes.
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Real-time Operating Systems - Seminar 2022/2023 Summer
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Real time operating systems - lectures & exercises, 2023 summer
e-Learning CoursesSupport course for real-time operating systems
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Real-time Operating Systems - L&E 2023/4
e-Learning CoursesWebsite supporting the course: "Real-time Operating Systems"
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Real Time Operating Systems - Lecture & exercises 2021/22L
e-Learning CoursesSupporting course for RTOS seminr classes.
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New PCR test for detection of Candida glabrata based on the molecular target chosen by the RAPD technique
PublicationRapid, reliable diagnosis is a necessary condition for the successful treatment of infections. Such diagnostic assays are continually being developed. #e paper presents a method for selecting the molecular target for PCR-based diagnostics based on the comparison of RAPD patterns. A sequence encoding Candida glabrata CBS138 hypothetical protein was selected. The limit of detection for PCR and real-time PCR reactions with DNA extracted...
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Antiviral activity of bee bread derived from polish apiaries.
Open Research DataBee bread is a product of fermentation of bee-collected pollen and revealed a high nutritional value. Other bee products, such as honey and propolis, are known for their antiviral activity, but bee bread is still under investigation, thus its antiviral potential is still unspecified. For investigation antiviral activity of bee bread samples, cytotoxicity...
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Rapid Assays for Specific Detection of Fungi of Scopulariopsis and Microascus Genera and Scopulariopsis brevicaulis Species
PublicationPurpose Fungi of Scopulariopsis and Microascus genera cause a wide range of infections, with S. brevicaulis being the most prevalent aetiological agent of mould onychomycosis. Proper identification of these pathogens requires sporulating culture, which considerably delays the diagnosis. So far, sequencing of rDNA regions of clinical isolates has produced ambiguous results due to the lack of reference sequences in publicly available...
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Molecular detection of Candida krusei
PublicationThe species identification of fungi belonging to genus Candida is an important issue as this genus becomes the emerging problem of nosocomial infections. As Candida krusei presents intrinsicresistance to the fluconazole that is the drug of the first choice in case of invasive candidiasis the PCR identifying the DNA of C. krusei was elaborated. The analytical sensitivity of the assay on spiked blood samples was estimated at 3-5...
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Konstrukcja nowych układów do diagnostyki molekularnej zakażeń o etiologii Candida spp.
PublicationDrożdżaki z rodzaju Candida należą do jednych z najczęściej izolowanych czynników etiologicznych zakażeń krwi. Szybka i bezbłędna identyfikacja gatunkowa pozwala na wdrożenie odpowiedniego leczenia i zwiększa szansę na powodzenie terapii. Celem badań była konstrukcja nowych układów wykorzystujących narzędzia diagnostyki molekularnej do specyficznej identyfikacji gatunkowej i rodzajowej drożdżaków Candida. Poszukiwano również nowych...
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Expression of cyclin D1 after treatment with doxorubicin in the HL-60 cell line
PublicationIncreased levels of cyclin D1 and amplification of CCND1 gene occur in many types of cancers. We have followed the expression of cyclin D1 after treatment with doxorubicin with reference to cell death and other possible therapeutic implications. The effect of the treatment on the cell cycle, survival, intracellular level (flow cytometry), and intracellular localization of cyclin D1 (fluorescence microscopy) and expression of...
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Olgun Aydin dr
PeopleOlgun Aydin finished his PhD by publishing a thesis about Deep Neural Networks. He works as a Principal Machine Learning Engineer in Nike, and works as Assistant Professor in Gdansk University of Technology in Poland. Dr. Aydin is part of editorial board of "Journal of Artificial Intelligence and Data Science" Dr. Aydin served as Vice-Chairman of Why R? Foundation and is member of Polish Artificial Intelligence Society. Olgun is...
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Structure and the Biochemical Potential of the Bacterial Community in Wastewater Treatment Plant Effluent and in Receiving Waters: Northern Poland Case Study
PublicationWastewater treatment processes are monitored mainly in terms of biogenic substance removal efficiency. Little is known about the treated wastewater microbiome and its influence on the recipient. In this study, a wide range of methods (cultivation, microscopic analysis, New Generation Sequencing and Real Time PCR) were employed to determine the bacterial community composition of the treated wastewater and its receiver. Preliminary...
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Molecular identification and genotyping of Staphylococci: genus, species, strains, colnes, lineages, and interspecies exchanges
PublicationStaphylococci are increasingly recognized as etiological agents of many opportunistic human and animal infections, emphasizing the need for a rapid and accurate identification, even to a genotypical level of these bacteria. In the recent years, there has been a significant progress in typing and phylogenetic study of Staphylococcus species. Here, we describe molecular methods used in taxonomy as well as staphylococci characterization....
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cDNA fingerprint from the hepatopancreatic glands of pond snails (Lymnaea stagnalis) exposed to benzo[a]pyrene
PublicationIdentification of differentially expressed genes that could be potentially used as biomarkers of PAH exposure of common invertebrate animal (like freshwater snail) would be a valuable resource for investigators interested in toxicology and biomonitoring of aquatic environments. Therefore, the aim of this research was to investigate effects of waterborne benzo[a]pyrene (B[a]P) exposure on mRNA expression in the pond snail’s (Lymnaea...
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Ewaluation of a PCR Melting Profile technique for bacterial strain differentiation
PublicationBadano przydatność techniki PCR MP w efektywnym typowaniu szczepów w badaniach epidemiologicznych zakażeń szpitalnych. Technika oparta jest na użyciu niskiej temperatury denaturacji podczas LM PCR. Badaniu zostały poddane izolaty Escherichia coli pochodzące od pacjentów Szpitala Klinicznego w Gdańsku. Wykazano, że technika jest szybka, o wysokim potencjale różnicującym i bardzo dobrej powtarzalności i może być zastosowana w badaniach...
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Optimized 5-hour multiplex PCR test for the detection of tinea unguium: performance in a routine PCR laboratory
PublicationWe recently reported the development of a 5-hour multiplex PCR test for the detection of tinea unguium and the optimization of this test by the inclusion of an inhibition control. Here we report the performance of this procedure as used in a routine clinical laboratory as compared to conventional microscopy and culture-based techniques performed in a mycology reference laboratory. We found in processing 109 samples that 22 (20.2%)...
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Principles and applications of Ligation Mediated PCR methods for DNA-based typing of microbial organisms
PublicationA significant number of DNA-based techniques has been introduced into the field of microorganisms’ characterization and taxonomy. These genomic fingerprinting methods were developed to detect DNA sequence polymorphisms by using general principles, such as restriction endonuclease analysis, molecular hybridization, and PCR amplification. In recent years, some alternative techniques based on ligation of oligonucleotide adapters before...
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A simple modification of PCR thermal profile applied to evade persisting contamination
PublicationThe polymerase chain reaction (PCR), one of the most commonly applied methods of diagnostics and molecular biology has a frustrating downside known as the false positive signal or contamination. Several solutions to avoid and to eliminate PCR contaminations have been worked out to date but the implementation of these solutions to laboratory practice may be laborious and time consuming. A simple approach to circumvent the problem...
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Genotyping Techniques for Determining the Diversity of Microorganisms
PublicationTyping of microbial pathogens, or identifying bacteria at the strain level, is particularly important for diagnosis, treatment, and epidemiological surveillance of bacterial infections. This is especially the case for bacteria exhibiting high levels of antibiotic resistance or virulence, and those involved in nosocomial or pandemic infections. Strain typing also has applications in studying bacterial population dynamics. The part...
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PCR-ELISA: inexpensive alternative to quantitative PCR
PublicationPCR-ELISA (polymerase chain reaction-enzyme linked immunosorbent assay), a combination of PCR and ELISA methods, has been used since late 1980s. The technique is based on specially labelled DNA fragments which are captured by specific DNA sequences and detected by antibodies. The whole procedure of PCR-ELISA is divided into three steps: DNA extraction, PCR reaction and detection by ELISA. The method has been found as very specific...
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Rozdział 5. Nowoczesne metody diagnostyki zakażeń grzybiczych wywoływanych przez dermatofity = Novel diagnostic methods of funfal infections caused by dermatophytes
PublicationDermatofity są głównym czynnikiem etiologicznym grzybic powierzchownych. Do tej klasy grzybów należą przedstawiciele trzech rodzajów: Epidermophyton, Microsporum i Trichophyton. Klasyczne metody diagnostyczne polegają na bezpośredniej ocenie mikroskopowej oraz badaniu hodowlanym. Identyfikacja gatunkowa jest możliwa na podstawie cech makroskopowych kolonii: ich struktury, koloru, kształtu, oraz obserwacji mikroskopowych (kształt...
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Józef Kotus dr hab. inż.
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Techniki amplifikacji kwasów nukleinowych -2022-2023
e-Learning CoursesSpecjalność: Biotechnologia molekularna (WCh), II stopnia, stacjonarne Prowadzący wykład: dr hab. Beata Krawczyk, prof. uczelni - forma stacjonarna Prowadzący laboratoria: mgr inż. Bartosz Ostrowski - zajęcia stacjonarne na PG, sala 17 WCH C. Kontakt: (58) 347-23-83 Beata Krawczyk (wykłady): e-mail: beata.krawczyk@pg.edu.pl; WCH B; p.218; Bartosz Ostrowski, WCH B, p.218 tel. (58) 347-13-83 Konsultacje: stacjonarnie we wtorki ...
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Techniki amplifikacji kwasów nukleinowych -2021-2022
e-Learning CoursesSpecjalność: Biotechnologia molekularna (WCh), II stopnia, stacjonarne Prowadzący wykład: dr hab. Beata Krawczyk, prof. uczelni - stacjonarnie na uczelni Prowadzący laboratoria: mgr inż. Magdalena Fordon - zajęcia stacjonarne na PG, sala 17 WCH C. Kontakt: (58) 347-23-83 Beata Krawczyk (wykłady): e-mail: beata.krawczyk@pg.edu.pl; WCH B; p.218; Magdalena Fordon (laboratoria): magda.fordon@gmail.com; WCH B; p.218 Konsultacje:...
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Techniki Amplifikacji Kwasów Nukleinowych -2023-2024
e-Learning CoursesSpecjalność: Biotechnologia molekularna (WCh), II stopnia, stacjonarne Prowadzący wykład: dr hab. Beata Krawczyk, prof. uczelni - forma stacjonarna Prowadzący laboratoria: mgr inż. Bartosz Ostrowski - zajęcia stacjonarne na PG, sala 209 WCH B (bud.7); II piętro. Kontakt: (58) 347-23-83 Beata Krawczyk (wykłady): e-mail: beata.krawczyk@pg.edu.pl; WCH B; p.217;tel. (58) 347-23-83 Bartosz Ostrowski, WCH B, p.218 tel. (58) 347-13-83 Konsultacje:...
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ADSRRS-fingerprinting and PCR MP techniques for studies of intraspecies genetic relatedness in Staphylococcus aureus
PublicationW pracy pokazano użyteczność dwóch nowych metod do typowania molekularnego Staphylococcus aureus, ADSRRS-fingerprinting (Amplification of DNA fragments Surrounding Rare Restriction Sites) i PCR MP (PCR melting profile). Przebadano 37 szczepów Staphylococcus aureus izolowanych od pacjentów z czyracznością. Jako metodę referencyjną zastosowano REA-PFGE. Okazało się, że izolaty Staphylococcus aureus z nosa od niektórych pacjentów...
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PCR melting profile (PCR MP) - a new tool for differentiation of Candida albicans strains
PublicationPo raz pierwszy przedstawiliśmy użyteczność techniki PCR MP opartej na metodzie ligacji adaptorów (LM PCR) i obniżonej temperaturze denaturacji w cyklach PCR do wewnątrzgatunkowego różnicowania szczepów Candida albicans. Przebadaliśmy 123 szczepy Candida albicans z zastosowaniem trzech metod genotypowania: PCR MP, REA-PFGE i RAPD i uzyskaliśmy odpowiednio 27, 26 i 25 unikalnych genotypów. Zaprezentowane dane pokazały, że technika...
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Michał Mazur dr inż.
PeopleAktualne zainteresowania inżynieria mechaniczna, robotyka, drgania mechaniczne, analiza modalna, sterowanie, systemy czasu rzeczywistego Wybrane publikacje Kaliński K., Galewski M., Mazur M., Chodnicki M, 2017, Modelling and Simulation Of A New Variable Stiffness Holder for Milling Of Flexible Details, Polish Maritime Research, vol 24, ss. 115-124 Kaliński K. J., Mazur M.: Optimal control at energy performance index of the mobile...
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Modified DNA polymerases for PCR troubleshooting
PublicationPCR has become an essential tool in biological science. However, researchers often encounter problems with difficult targets, inhibitors accompanying the samples, or PCR trouble related to DNA polymerase. Therefore, PCR optimization is necessary to obtain better results. One solution is using modified DNA polymerases with desirable properties for the experiments. In this article, PCR troubleshooting, depending on the DNA polymerase...
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The New LM-PCR/Shifter Method for the Genotyping of Microorganisms Based on the Use of a Class IIS Restriction Enzyme and Ligation Mediated PCR
PublicationThis study details and examines a novel Ligation-Mediated - Polymerase Chain Reaction (LM-PCR) method. Named the LM-PCR/Shifter, it relies on the use of a Class IIS restriction enzyme giving restriction fragments with different 4 base, 5' overhangs, this being the Shifter, and the ligation of appropriate oligonucleotide adapters. A sequence of 4-base, 5' overhangs of the adapter and a 4-base sequence of the 3' end of the primer(s)...
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Technika PCR MP (PCR Melting Profile) - możliwości zastosowania w badaniach epidemiologicznych
PublicationUżyteczność PCR MP (ang. PCR Melting Profile), stosunkowo nowej techniki różnicowania genetycznego szczepów mikroorganizmów, pokazano na przykładach badań epidemiologicznych, dotyczących wykrywania zakażeń szpitalnych, analizy sytuacji epidemiologicznej i identyfikacji transmisji bakteryjnej u poszczególnych pacjentów. Technikę PCR MP charakteryzuje prostota, niskie koszty wykonania oraz potencjał różnicujący porównywalny do metody...
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Molecular typing of clinical isolates of Escherichia coli by a new PCR MP method
PublicationTypowanie epidemiologiczne bakterii jest rutynową procedurą stosowaną w badaniach zakażeń szpitalnych i wymaga stosowania odpowiednich metod o wysokim potencjale różnicującym. Przedstawiliśmy możliwości nowej techniki PCR MP w różnicowaniu szczepów E. coli. Rezultaty zostały porównane z wynikami uzyskanymi metodą PFGE.