Filters
total: 882
-
Catalog
- Publications 560 available results
- Journals 30 available results
- Publishing Houses 1 available results
- People 22 available results
- Inventions 1 available results
- Projects 8 available results
- Research Teams 3 available results
- e-Learning Courses 21 available results
- Events 1 available results
- Open Research Data 235 available results
Search results for: EKSPRESJA GENU
-
Virulence gene profiles in Staphylococcus aureus isolated from cows with subclinical mastitis in eastern Poland
PublicationStaphylococcus aureus is arguably the most important pathogen involved in bovine mastitis. The aim of this study was to determine the virulence gene profiles of 124 Staph. aureus isolates from subclinical mastitis in cows in eastern Poland. The presence of 30 virulence genes encoding adhesins, proteases and superantigenic toxins was investigated by PCR. The 17 different combinations of adhesin genes were identified. Occurrence...
-
The Relationship between Gene Activity and Nitrous Oxide Production during Nitrification in Activated Sludge Systems
PublicationNitrite is an important factor which inhibits the first stage of the nitrification, the effect is stronger with the decrease of the DO concentrations. NO2- presence at concentration above 15 mg N – NO2 in the aeration tanks stimulates N2O production regardless of the DO concentration. The significant nirS gene induction, observed especially during the experiments with the nitrite addition indicates that N2O production is basically...
-
Characterization of immobilized Escherichia coli cells transformed by β-galactosidase gene from Pyrococcus woesei
PublicationThermostable β-galactosidase from Escherichia coli transformant containing theenzyme gene from Pyrococcus woesei was immobilized on alginate gel.The benefits of using whole bacterial cells not only exclude expensive, laborious proteinisolation and purification but also stabilize enzymes by cytosol components. Increase inproductivity of enzyme can be achieved by cells permeabilization. To increase permeability ofcytoplasmic membrane...
-
NADPH Oxidase Gene Polymorphism is Associated with Mortality and Cardiovascular Events in 7-Year Follow-Up
PublicationThe CYBA gene encodes the regulatory subunit of NADPH oxidase, which maintains the redox state within cells and in the blood vessels. That led us to investigate the course of coronary artery disease (CAD) with regards to CYBA polymorphisms. Thus, we recruited 1197 subjects with coronary atherosclerosis and observed them during 7-year follow-up. Three CYBA polymorphisms: c.214C>T (rs4673), c.-932G>A (rs9932581), and c.*24G>A (1049255)...
-
Phylogenetic trees of genus Oncidium Sw. based on analysis of DNA sequences
Open Research DataGenus Oncidium Sw. is widely regarded as a polyphiletic, and the taxonomic boundaries between him and such genera as Odontoglossum Kunth. or Miltonia Lindley remain blurred. The goal of the study was to determine the phylogenetic relationships within the genus Oncidium s.lato based on the DNA sequences analysis. The correlation between molecular data...
-
GENE THERAPY
Journals -
Comparison of Chemical Composition and Colour Parameters of Different Mentha Genus Plants Grown under Organic Conditions
Publication -
Gene and Protein Accumulation Changes Evoked in Porcine Aorta in Response to Feeding with Two Various Fructan Sources
Publication -
Clinical and Biological Significance of ESR1 Gene Alteration and Estrogen Receptors Isoforms Expression in Breast Cancer Patients
Publication -
Comparative Analysis of MicroRNA-Target Gene Interaction Prediction Algorithms Based on Integrated P-Value Calculation
Publication -
Comprehensive Analysis of MILE Gene Expression Data Set Advances Discovery of Leukaemia Type and Subtype Biomarkers
Publication -
Comparative Analysis of microRNA-Target Gene Interaction Prediction Algorithms - The Attempt to Compare the Results of Three Algorithms
Publication -
Selected mice models based on APP, MAPT and presenilin gene mutations in research on the pathogenesis of Alzheimer’s disease
Publication -
Up-regulation of ferritin ubiquitination in skeletal muscle of transgenic rats bearing the G93A hmSOD1 gene mutation
Publication -
A dependency between PRL gene polymorphism and breeding parameters in Polish Black-and-White Holstein-Friesian cows
Publication -
A novel method of Mycobacterium tuberculosis complex strain differentiation using polymorphic GC-rich gene sequences.
PublicationTuberculosis is one of the leading infectious diseases. In this work, a new genotyping method of Mycobacterium tuberculosis (Mtb) complex strain is presented. 27 Mtb genomes were analyzed for the presence of length polymorphism within polymorphic GC-rich gene sequences. Four genes, Rv3345c, Rv3507, Rv0747 and Rv3511, showing variation in length depending on the Mtb strain were selected for designing primer sequences flanking variable...
-
Biochimica et Biophysica Acta-Gene Regulatory Mechanisms
Journals -
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION
Journals -
XbaI and PvuII Polymorphisms of Estrogen Receptor 1 Gene in Females with Idiopathic Scoliosis: No Association with Occurrence or Clinical Form
Publication -
Methylation level of the regulatory regions of the estrogen receptor type 1 gene in paravertebral muscles of girls with idiopathic scoliosis
Publication -
Fluorescence resonance energy transfer usage to track the sequence promoter changes in CGB5 gene in ovarian cancer patients
Publication -
Methylation of Estrogen Receptor 1 Gene in the Paraspinal Muscles of Girls with Idiopathic Scoliosis and Its Association with Disease Severity
Publication -
Deep analysis of Loop L1 HVRs1-4 region of the hexon gene of adenovirus field strains isolated in Poland
Publication -
Association of LBX1 Gene Methylation Level with Disease Severity in Patients with Idiopathic Scoliosis: Study on Deep Paravertebral Muscles
Publication -
The Role of Dimethyl Sulfoxide (DMSO) in Gene Expression Modulation and Glycosaminoglycan Metabolism in Lysosomal Storage Disorders on an Example of Mucopolysaccharidosis
Publication -
Genetic variation in C-reactive protein (CRP) gene is associated with retinopathy and hypertension in adolescents with type 1 diabetes
Publication -
Hydrolytic and transgalactosylation activities of immobilized Escherichia coli cells transformed by β-galactosidase gene from Pyrococcus woesei
PublicationPrzedstawiono wyniki badań dotyczących skuteczności transformacji laktozy katalizowanej immobilizowanymi w żelu alginianowym rekombinantowymi komórkami Escherichia coli, transformowanymi genem b-galaktozydazy z Pyrococcus woesei. Wykazano, że aktywność hydrolityczna rekombinantowych komórek wzrasta o 35% po permeabilizacji w środowisku 40% etanolu. Optymalna temperatura i pH katalizowanej reakcji wynosiły odpowiednio 98C i 5,4....
-
A new cold-active β-galactosidase from Arthrobacter sp. S3* - gene cloning, overexpression, purification and properties
PublicationA psychrotrophic bacterium producing a cold-active β-galactosidase was isolated from Spitsbergen soil and classified as Arthrobacter sp. S3*. The gene encoding β-galactosidase was isolated from the genomic DNA library, sequenced, cloned, expressed in Escherichia coli, purified by ion exchange chromatography and characterized. The Arthrobaster sp. S3* β-galactosidase is a homotrimeric enzyme composed of 74,4 kDa subunits. It is...
-
Vitamin D Receptor Gene Polymorphism and Vitamin D Status in Population of Patients with Cardiovascular Disease—A Preliminary Study
Publication -
Amplification of c-myc gene and overexpression of c-Myc protein in breast cancer and adjacent non-neoplastic tissue
Publication -
Combining CDKN1A gene expression and genome-wide SNPs in a twin cohort to gain insight into the heritability of individual radiosensitivity
Publication -
Changes in Nuclear Gene Expression Related to Mitochondrial Function Affect Extracellular Matrix, Collagens, and Focal Adhesion in Keratoconus
Publication -
Species - specific detection of the toxic mushrooms Amanita virosa and Amanita verna by PCR amplification of the gpd gene fragment
PublicationZaprojektowano dwie pary starterów do reakcji PCR, które umożliwiają gatunkowo specyficzną amplifikację unikalnych fragmentów genów gpd dla dwóch grzybów kapeluszowych: muchomora wiosennego (Amanita verna) i muchomora jadowitego (Amanita virosa).
-
Induction of immune responses in sheep by vaccination with liposome-entrapped DNA complexes encoding Toxoplasma gondii MIC3 gene
PublicationToxoplasma gondii is a parasite that has been extensively studied due to its medical and veterinary importance in terminating pregnancies. Consequently, a satisfactory vaccine is required to control itsadverse effects on pregnant animals. The microneme protein, MIC3, is a major adhesion protein that binds to the surface of host cells and parasites, and is therefore a potential vaccine against T. gondii.The viability of MIC3 as...
-
Sequence variants of p21 gene of the MRL/MpJ mouse: a possible association with the deficit of p21 protein and regenerative phenotype.
PublicationThe MRL/MpJ mouse is known for its enhanced regeneration abilities that manifested themselves by scarless ear-hole wound closure. The deficit of p21 found in the MRL is associated with the regenerative capacity as the p21 gene knockout in a mouse strain unrelated to the MRL mouse results in an ear-hole closure effect, similar to that observed in the MRL mouse. Cdkn1a gene encodes p21 protein, a cyclin-dependent kinase inhibitor,...
-
Isolation of the GFA1 gene encoding glucosamine-6-phosphate synthase of Sporothrix schenckii and its expression in Saccharomyces cerevisiae
PublicationGlucosamine-6-phosphate synthase (GlcN-6-P synthase) is an essential enzyme involved in cell wall biogenesis that has been proposed as a strategic target for antifungal chemotherapy. Here we describe the cloning and functional characterization of Sporothrix schenckii GFA1 gene which was isolated from a genomic library of the fungus. The gene encodes a predicted protein of 708 amino acids that is homologous to GlcN-6-P synthases...
-
Neotenic phenomenon in gene expression in the skin of Foxn1- deficient (nude) mice - a projection for regenerative skin wound healing
PublicationMouse fetuses up to 16 day of embryonic development and nude (Foxn1- deficient) mice are examples of animals that undergo regenerative (scar-free) skin healing. The expression of transcription factor Foxn1 in the epidermis of mouse fetuses begins at embryonic day 16.5 which coincides with the transition point from scar-free to scar-forming skin wound healing. In the present study, we tested the hypothesis that Foxn1 expression...
-
A novel cold-active beta-D-galactosidase from the Paracoccus sp. 32d - gene cloning, purification and characterization
PublicationBeta-D-galactosidase (EC 3.2.1.23) catalyze the hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides. Cold-active beta-D-galactosidases have recently become a focus of attention of researchers and dairy product manufactures owing to theirs ability to: (I) eliminate of lactose from refrigerated milk for people afflicted with lactose intolerance, (II) convert lactose to glucose and galactose which...
-
Post-Effort Changes in Autophagy- and Inflammation-Related Gene Expression in White Blood Cells of Healthy Young Men
PublicationAcute, strenuous physical exertion requiring high levels of energy production induces the production of reactive oxygen species and metabolic disturbances that can damage the mitochondria. Thus, selective autophagic elimination of defective mitochondria may improve resistance to oxidative stress and potentially to inflammation. The main goal of this study was to evaluate the impacts of intense effort on changes in the expression...
-
Józef Kur prof. dr hab.
People -
MOST IM. GEN. GROTA-ROWECKIEGO W WARSZAWIE – ANALIZY POMOSTU TECHNIKĄ ,,TREPANACJI”
PublicationW pracy przedstawione są wybrane zagadnienia związane z analizą obliczeniową modernizowanego mostu przez Wisłę im. gen Stefana Grota-Roweckiego w Warszawie. Obiekt jest stalową konstrukcją o schemacie belki ciągłej i całkowitej długości 646 m. Po przebudowie most ma być przeznaczony dla ruchu drogowego i pieszo-rowerowego o parametrach drogi ekspresowej S-8. W programie funkcjonalno–użytkowym założono znaczne poszerzenie obiektu,...
-
MOST IM. GEN. GROTA-ROWECKIEGO W WARSZAWIE – ANALIZY POMOSTU TECHNIKĄ ,,TREPANACJI”
PublicationW pracy przedstawione są wybrane zagadnienia związane z analizą obliczeniową modernizowanego mostu przez Wisłę im. gen Stefana Grota-Roweckiego w Warszawie. Obiekt jest stalową konstrukcją o schemacie belki ciągłej i całkowitej długości 646 m. Po przebudowie most ma być przeznaczony dla ruchu drogowego i pieszo-rowerowego o parametrach drogi ekspresowej S-8. W programie funkcjonalno–użytkowym założono znaczne poszerzenie obiektu,...
-
Kreowanie wartości publicznej przez wykorzystanie wolnego i otwartego oprogramowania GNU Health
PublicationGłównym celem pracy jest prezentacja roli, potencjału i funkcjonalności oprogramowania GNU Health. Ten zaawansowany i dojrzały, wysokiej jakości produkt - opracowany i nieustannie rozwijany w ramach projektu wolnego i otwartego oprogramowania - jest z sukcesem wdrażany w placówkach ochrony zdrowia w wielu krajach. Jego zastosowanie przyczynia się nie tylko do usprawnień w zarządzaniu praktyką medyczną lecz również odgrywa ważną...
-
REPORT Verification expertise of Detroit Diesel engines 16V149TI on the vessel ORP GEN. PUŁASKI
PublicationVerification expertise of propulsion engines for generating sets of the ORP GEN. PUŁASKI - type 16V149TI related to a complaint sent by the shipowner to Shiprepair Yard NAUTA S.A.
-
GRA1 protein vaccine confers better immune response compared to codon-optimized GRA1 DNA vaccine
PublicationW publikacji oszacowano immunogenność i właściwości protekcyjne szczepionki anty-Toxoplasma gondii opartej na białku rekombinantowym GRA1 oraz dwóch rodzajach szczepionek DNA (plazmidu zawierającego gen kodujący białko GRA1 dzikiego typu oraz modyfikowanego genu kodującego białko GRA1). Uzyskane wyniki sugerują lepsze właściowści immunoprotekcyjne rekombinantowego białka antygenowego niż szczepionek DNA.
-
Polymorphism in the chemokine receptor 7 gene (CCR7) is associated with previous myocardial infarction in patients undergoing elective coronary angiography
Publication -
DNA methylation analysis with methylation‐sensitive high‐resolution melting (MS‐HRM) reveals gene panel for glioma characteristics
Publication -
Angiotensin converting enzyme gene polymorphism is associated with severity of coronary artery disease in men with high total cholesterol levels
Publication -
An insertion/deletion polymorphism of the dihydrofolate reductase (DHFR) gene is associated with serum and red blood cell folate concentrations in women
Publication -
Identification of High-Risk Stage II Colorectal Tumors by Combined Analysis of the NDRG1 Gene Expression and the Depth of Tumor Invasion
Publication