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total: 399
Search results for: glicyna
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The impact of land use and season on the riverine transport of mercury into the marine coastal zone
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Mercury loads into the sea associated with extreme flood
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Distribution of mercury in different environmental compartments in the aquatic ecosystem of the coastal zone of the Southern Baltic Sea
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Design of selective substrates of proteinase 3 using combinatorial chemistry methods
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Efficient production of the Toxoplasma gondii GRA6, p35 and SAG2 recombinant antigens and their applications in the serodiagnosis of toxoplasmosis
PublicationSkonstruowano system ekspresji rekombinantowych białek antygenowych GRA6, p35 i SAG2 Toxoplasma gondii. Uzyskano miligramowe ilości białek fuzyjnych po zastosowaniu jednoetapowego oczyszczania z wykorzystaniem metody chromatografii metalopowinowactwa. Przydatność otrzymanych antygenów do konstrukcji serologicznego testu diagnostycznego oszacowano z wykorzystaniem testu ELISA, w którym zastosowano 99 surowic pobranych od pacjentów...
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A new human IgG avidity test, using mixtures of recombinant antigens (rROP1, rSAG2, rGRA6), for the diagnosis of difficult-to-identify phases of toxoplasmosis
PublicationThe preliminary diagnostic utility of two mixtures of Toxoplasma gondii recombinant antigens (rROP1+rSAG2 and rROP1+rGRA6) in IgG ELISA and IgG avidity test has been evaluated. A total of 173 serum samples from patients with toxoplasmosis and seronegative people were examined. The sensitivity of IgG ELISA for rROP1+rSAG2 and rROP1+rGRA6 was 91.1% and 76.7%, respectively, while the reactivity for sera from patients where acute toxoplasmosis...
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Epitope Mapping of BmpA and BBK32 Borrelia burgdorferi Sensu Stricto Antigens for the Design of Chimeric Proteins with Potential Diagnostic Value
PublicationLyme disease is a tick-borne zoonosis caused by Gramnegative bacteria belonging to the Borrelia burgdorferi sensu lato (s.l.) group. In this study, IgM- and IgG-specific linear epitopes of two B. burgdorferi sensu stricto (s.s.) antigens BmpA and BBK32 were mapped using a polypeptide array. Subsequently, two chimeric proteins BmpABBK32-M and BmpA-BBK32-G were designed to validate the construction of chimeras using the identified...
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Specjalne Techniki Betonowania 2023
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Podniesienie jakości przestrzeni kampusu PG poprzez wysokiej jakości architekturę i wprowadzenie rozwiązań proekologicznych
e-Learning CoursesProjekt realizowany będzie w 8 zespołach warsztatowych (3-4 studentów WA + student inżynierii środowiska WILIŚ). 4 zespoły przygotują propozycję przebudowy Gmachu B Politechniki Gdańskiej wraz z opracowaniem krajobrazowym fragmentu terenu kampusu jako laboratorium innowacyjnych rozwiązań technologicznych i środowiskowych. Zadanie będzie polegało na znalezieniu ciekawej formuły ukształtowania (rozbudowy) strefy przyziemniej...
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Research project: CAD. Integrated archictural design + Methodology of scientific work 2022/2023
e-Learning CoursesKurs e-learningowy stanowi uzupełnienie zajęć stacjonarnych Projekt Naukowy (Techniki Komputerowe i Metodyka Pracy Naukowej) dla studentów II stopnia (2 semestr) Zajęcia dotyczą zastosowania środowiska GIS i oprogramowania BIM oraz odpowiednich metod naukowych w procesie projektowania i przekształcania obszarów waterfrontów.
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Biologia molekularna_laboratorium 2023/2024
e-Learning CoursesKurs jest formą dydaktyczną, która ma na celu uzupełnienie zajęć laboratoryjnych z przedmiotu "Biologia molekularna" prowadzonych w roku akademickim 2023/2024 w sposób stacjonarny na uczelni.
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PG_00050844 Projekt Architektoniczny II sem. 2 mgr_20/21_EDU CENTRUM ŁEBA
e-Learning CoursesKurs przeznaczony dla studentów sem. 2 stopnia II realizujących przedmiot Projekt Architektoniczny II w Katedrze Architektury Miejskiej i Przestrzeni Nadwodnych.
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Similarities among glacials and interglacials in the LR04 benthic oxygen isotope stack over the last 1.014 million years revealed by cluster analysis and a DTW algorithm
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Elemental and organic carbon in aerosols over urbanized coastal region (southern Baltic Sea, Gdynia)
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Proteomic analysis of stipe explants reveals differentially expressed proteins involved in early direct somatic embryogenesis of the tree fern Cyathea delgadii Sternb
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The Development of Toxoplasma gondii Recombinant Trivalent Chimeric Proteins as an Alternative to Toxoplasma Lysate Antigen (TLA) in Enzyme-Linked Immunosorbent Assay (ELISA) for the Detection of Immunoglobulin G (IgG) in Small Ruminants
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Gold Nanoparticles (AuNPs)—Toxicity, Safety and Green Synthesis: A Critical Review
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The immune responses of sheep after DNA immunization with Toxoplasma gondii MAG1 antigen - with and without co-expression of ovine interleukin 6
PublicationW niniejszej pracy porównano odpowiedź immunologiczną owiec, którym podano domięśniowo plazmid DNA zawierający gen kodujący białko macierzy cyst tkankowych (MAG1) Toxoplasma gondii - pojedynczo bądź z DNA plazmidu kodującego owczą interleukinę 6 (IL-6). Dzięki wykorzystaniu plazmidu zawierającego gen mag1 odnotowano podwyższony poziom przeciwciał IgG1 oraz IgG2 w surowicach szczepionych zwierząt.
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Application of recombinant antigens in serodiagnosis of swine toxoplasmosis and prevalence of Toxoplasma gondii infection among pigs in Poland
PublicationNiniejsza praca przedstawia częstość występowania toksoplazmozy w populacji świń obejmującej 1754 osobników pochodzących z różnych regionów Polski, która określona została z wykorzystaniem testu IgG ELISA opartego na antygenie natywnym pasożyta. Specyficzne przeciwciała anty-T. gondii klasy IgG zostały wykryte u 19,2% zwierząt z testowanej grupy. Ponadto w pracy oszacowano użyteczność diagnostyczną w rozpoznawaniu toksoplazmozy...
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Zero-Emissions, Off-grid, Autonomous Houseboat – a Case Study of Selected Locations in Europe
PublicationThe aim of this study was to assess the feasibility of creating a prototype of a self-sufficient houseboat that is capable of prolonged independence from power grids and freshwater sources. In this design, electricity demand is met by a photovoltaic installation, and the energy is stored in batteries. Fresh water for living needs may be obtained from many sources, depending on the environmental conditions, such as through purifying...
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Immunogenic and protective activity of recombinant chimeric Toxoplasma gondii proteins
PublicationToxoplasmosis, one of the most common parasitic invasions worldwide, may pose a great threat for individuals with weakened immune system. The parasite also causes considerable economic losses due to infection of livestock. To date there is no efficient immunoprophylaxis for humans and animals, which would provide long-lasting protection against T. gondii invasion. Chimeric proteins, containing several selected fragments of parasite...
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The first study on the usefulness of recombinant tetravalent chimeric proteins containing fragments of SAG2, GRA1, ROP1 and AMA1 antigens in the detection of specific anti-Toxoplasma gondii antibodies in mouse and human sera
PublicationThis study presents an evaluation of four tetravalent recombinant chimeric proteins containing fragments of the Toxoplasma gondii antigens, SAG2, GRA1, ROP1 and AMA1, as potential replacements of a the soluble, whole-cell tachyzoite lysate (TLA) used in serological assays. Recombinant chimeric proteins (SAG2-GRA1-ROP1-AMA1N, AMA1N-SAG2-GRA1-ROP1, AMA1C-SAG2-GRA1-ROP1, and AMA1-SAG2-GRA1-ROP1) obtained by genetic engineering were...
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Zespół Katedry Architektury Miejskiej i Przestrzeni Nadwodnych
Research TeamsWszystkie programy badań prowadzone w Katedrze powstają w odpowiedzi na problemy środowiskowe, wiążą się ze zmianami demograficznymi i wyzwaniami wynikającymi z rozwoju miast. Badane są urbanistyczne i architektoniczne możliwości związane z przekształceniami terenów poprzemysłowych. Zespół katedralny zaangażowany jest w opracowywanie kreatywnych rozwiązań dla ponownego wykorzystania struktur historycznych w oparciu o koncepcję...
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Mykologia i parazytologia molekularna 2023/2024
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Advanced Assessment of the Endogenous Hormone Level as a Potential Biomarker of the Urogenital Tract Cancer
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A targeted mass spectrometry immunoassay to quantify osteopontin in fresh-frozen breast tumors and adjacent normal breast tissues
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Status and trends of mercury pollution of the atmosphere and terrestrial ecosystems in Poland
PublicationThe goal of this paper is to assess the current status and trends of total mercury (THg) contamination of the atmosphere and terrestrial ecosystems in Poland. The study shows that the reduced domestic and worldwide atmospheric emission of Hg resulted in decreased THg level in the terrestrial biotope and biosphere. Considering that Poland is one of the main Hg emitters in Europe, the THg concentrations in its abiotic environment...
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The Osteopontin Tissue Level as a Breast Cancer Biomarker in Females After Mastectomy Measured by the Capillary Gel Electrophoresis Technique
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Mercury and Chlorinated Pesticides on the Highest Level of the Food Web as Exemplified by Herring from the Southern Baltic and African Penguins from the Zoo
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Cutaneous larva migrans imported from a tropical trip – Case report and literature review
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The Effects of Two Kinds of Dietary Interventions on Serum Metabolic Profiles in Haemodialysis Patients
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The immune responses of sheep after DNA immunization with, Toxoplasma gondii MAG1 antigen—with and without co-expression of ovine interleukin 6
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The Development of Toxoplasma gondii Recombinant Trivalent Chimeric Proteins as an Alternative to Toxoplasma Lysate Antigen (TLA) in Enzyme-Linked Immunosorbent Assay (ELISA) for the Detection of Immunoglobulin G (IgG) in Small Ruminants
PublicationThis study presents an evaluation of seventeen newly produced recombinant trivalent chimeric proteins (containing the same immunodominant fragment of SAG1 and SAG2 of Toxoplasma gondii antigens, and an additional immunodominant fragment of one of the parasite antigens, such as AMA1, GRA1, GRA2, GRA5, GRA6, GRA7, GRA9, LDH2, MAG1, MIC1, MIC3, P35, and ROP1) as a potential alternative to the whole-cell tachyzoite lysate (TLA) used...
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Evaluation of long‑term immunity and protection against T. gondii after immunization with multivalent recombinant chimeric T. gondii proteins
PublicationToxoplasmosis caused by the opportunistic, cosmopolitan protozoan Toxoplasma gondii is one of the most common parasitoses in the world. Although it may prove dangerous or even fatal for immunocompromised individuals, immunoprophylaxis for humans is still nonexistent. Thus, the aim of the current work was to assess the ability of two immunogenic recombinant chimeric T. gondii proteins, SAG2-GRA1-ROP1 (SGR) and SAG1-MIC1-MAG1-GRA2...
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Methodology of research on the impact of ITS services on the safety and efficiency of road traffic using transport models
PublicationThe current assessment of the impact of Intelligent Transport System (ITS) services on the level of traffic safety and efficiency is based mainly on expert assessments, statistical surveys or several traffic safety models requiring development. There is no structured, uniform assessment method that would give the opportunity to compare the impact of ITS services and their different configurations. The paper presents the methodology...
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Lucyna Damaszk
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Lucyna Nagórska
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Coincidence of PTPN22 c.1858CC and FCRL3 -169CC genotypes as a biomarker of preserved residual β-cell function in children with type 1 diabetes
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Species differences in total mercury concentration in gulls from the Gulf of Gdansk (Southern Baltic)
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High pressure impact on changes in potato starch granules
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Induction of immune responses in sheep by vaccination with liposome-entrapped DNA complexes encoding Toxoplasma gondii MIC3 gene
PublicationToxoplasma gondii is a parasite that has been extensively studied due to its medical and veterinary importance in terminating pregnancies. Consequently, a satisfactory vaccine is required to control itsadverse effects on pregnant animals. The microneme protein, MIC3, is a major adhesion protein that binds to the surface of host cells and parasites, and is therefore a potential vaccine against T. gondii.The viability of MIC3 as...
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Materiały Budowlane i Technologia Betonu - 2023/24
e-Learning CoursesZapoznanie z klasyfikacją cech technicznych materiałów budowlanych w zależności od ich rodzaju i przeznaczenia. Analiza wybranych właściwości wyrobów materiałów określających ich przydatność zastosowania w budownictwie ogólnym.
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Inżynieria ruchu drogowego - 2021-2022
e-Learning CoursesKurs z Inżynierii ruchu na kierunku Transport (sem. 5), semestr zimowy 2021/2022.Koordynator przedmiotu: dr inż. Aleksandra Romanowska
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Elective design I: Cultural Heritage Routes – Gdańsk Fortification System - 2022/2023 Project PROMETHEUS
e-Learning CoursesElective design I:Cultural Heritage Routes – Gdańsk Fortification Systemfor MSc in Arch. Seminars & assignments: 30 hTeacher: akub Szczepański, Justyna Borucka, Robert Hirsch Piotr Samól, Szymon Kowalski, Joanna Badach + Visiting Prof. Sandro Parrinello (from Pavia University) Cultural Heritage Routes – Gdańsk Fortification System This course explores challenges, conflicts and concepts of cultural heritage preservation...
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Materiały budowlane z technologią betonów - 2022/23
e-Learning CoursesZapoznanie z klasyfikacją i oznaczaniem cech technicznych materiałów budowlanych, składników betonu, mieszanek betonowych i stwardniałych betonów; dobór składników betonu i ustalanie składu betonu, klasyfikacja i stosowanie betonów, podstawowe procesy technologiczne w produkcji betonu.
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Steroid profiles as potential biomarkers in patients with urogenital tract cancer for diagnostic investigations analyzed by liquid chromatography coupled to mass spectrometry
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Influence of ozonation and biodegradation on toxicity of industrial textile wastewater
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Modulation of immune response to Toxoplasma gondii in sheep by immunization with a DNA vaccine encoding ROP1 antigen as a fusion protein with ovine CD154
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Mercury in the Polish part of the Baltic Sea: A response to decreased atmospheric deposition and changing environment
PublicationOur review of the literature showed that since the beginning of the socio-economic transformation in Poland in the 1990s, the downward trend in Hg emissions and its deposition in the southern Baltic Sea was followed by a simultaneous decrease in Hg levels in water and marine plants and animals. Hg concentrations in the biota lowered to values that pose no or low risk to wildlife and seafood consumers. However, in the first decade...
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Interplay between Selected Chemical and Biochemical Soil Properties in the Humus Horizons of Grassland Soils with Low Water Table Depth
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