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Wyniki wyszukiwania dla: cell culture
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A New Double Digestion Ligation Mediated Suppression PCR Method for Simultaneous Bacteria DNA-Typing and Confirmation of Species: An Acinetobacter sp. Model
PublikacjaWe have designed a new ddLMS PCR (double digestion Ligation Mediated Suppression PCR) method based on restriction site polymorphism upstream from the specific target sequence for the simultaneous identification and differentiation of bacterial strains. The ddLMS PCR combines a simple PCR used for species or genus identification and the LM PCR strategy for strain differentiation. The bacterial identification is confirmed in the...
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Praca z drewnem - Przestrzeń eksperymentu w tworzeniu architektury; Crafting Wood - Space of experiment in making architecture
PublikacjaW artykule przedstawiono eksperymenty z materiałem w tworzeniu architektury prowadzące do praktycznych rozwiązań w konstrukcji budynku. Takie podejście do tematyki zastosowania materiałów w architekturze nakreśla praktyczne aspekty tworzenia przestrzeni podczas pracy np. z drewnem. Podejście przedstawiono w kontekście dziedzictwa kulturowego podczas intensywnych warsztatów dotyczących tradycji i kultury budownictwa wybranych regionów...
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City and Urbanity in the Social Discourse
PublikacjaThe aim of our article is an attempt to present the concept of urbanity that has been shaped throughout centuries along with the development of European civilisation and now entered a new phase of social production of space based on cultural dimensions. The future of the majority of World’s population is connected currently with the urban life with the assumption that qualitative characteristics of life in the 21st century define...
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Possibility of Using Tea Fungus for Fermentation of Beetroot and Carrot Pomace Beverages
PublikacjaFermented beverages obtained from plant-based raw materials are becoming more popular due to their beneficial effects on human health. These include kombucha beverages, which are obtained by fermenting tea brew using the so-called tea fungus, which includes acetic acid bacteria and osmophilic yeast. A consortium of these microorganisms could also be used to prepare functional fermented beverages obtained from extracts of vegetable...
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Use of Daylight and Aesthetic Image of Glass Facades in Contemporary Buildings
PublikacjaThe paper deals with the architecture of contemporary buildings in respect to their aesthetic image created by the use of natural light. Sustainability is regarded as a principle of contemporary architecture, where daylighting is an important factor as it affects energy consumption and environmental quality of the space inside a building. Environmental awareness of architecture, however, involves a much wider and more holistic...
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The Main Buildings of Technical Universities in Lviv, Warsaw, Gdańsk and Wrocław. Neo-Renaissance „Architecture parlante”
PublikacjaThe article compares the buildings of historical campuses of four technical universities located in areas related to Polish culture. At the turn of the 19th and 20th centuries, several new technical universities were established in Central and Eastern Europe. In 1844, the Technical Academy was opened in Lviv. In 1899, the Russian who were occupying the east and central part of Poland established the Polytechnic Institute in Warsaw....
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"Shaping space" programme as a tool for educating youth about architecture
PublikacjaThe Polish Architectural Policy’s vision of a systematic promotion of spatial culture has made its way into the national curriculum for 2009 – 2016 designed for various stages of child and teenager education. The objective of this effort was to furnish a basis for a system of architectural education which allows teaching society to be more conscious in their decisions as to spatial order with the effect of improving the quality...
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Road Infrastructure Safety Management in Poland
PublikacjaThe objective of road safety infrastructure management is to ensure that when roads are planned, designed, built and used road risks can be identified, assessed and mitigated. Road transport safety is significantly less developed than that of rail, water and air transport. The average individual risk of being a fatality in relation to the distance covered is thirty times higher in road transport that in the other modes. This is...
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Changes in Functional and Spatial Layouts of Polish Single-Family Houses
PublikacjaArticle presents the evolution of functional and spatial layouts of single-family houses in Poland, with particular emphasis on the functions of a contemporary house. Singlefamily housing constitutes a significant part of the construction market in Poland. The way of forming functions in single-family buildings in the historical cycle has characteristic features resulting from the culture of the society in a given period. The functional...
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The usefulness of Toxoplasma gondii MIC1-MAG1-SAG1 chimeric antigen in the serodiagnosis of ovine toxoplasmosis
PublikacjaToxoplasmosis is a zoonotic disease caused by the protozoan Toxoplasma gondii, which infects humans and most warm-blooded animals throughout the world. Although human toxoplasmosis in healthy adults is usually asymptomatic, a serious disease can occur in the case of congenital infection and immunocompromised individuals. Among food animals, sheep, along with goats and pigs, possess the highest incidence of T. gondii cysts in meat,...
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Ogród muz II
Publikacja"Gdańsk nowożytny a świat antyczny" – to tytuł drugiego tomu publikacji stanowiącej zapis projektu "W nadbałtyckim ogrodzie muz…", zorganizowanego w roku 2015 przez PAN Bibliotekę Gdańską i Katedrę Filologii Klasycznej Uniwersytetu Gdańskiego. W tomie pierwszym, wydanym w 2016 roku pod tytułem W gdańskim ogrodzie muz, obok podstawowych dla recepcji kultury grecko-rzymskiej sfer ekspresji, jaką jest poezja i sztuka perswazji,...
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Gdańsk nowożytny a świat antyczny
Publikacja"Gdańsk nowożytny a świat antyczny" – to tytuł drugiego tomu publikacji stanowiącej zapis projektu "W nadbałtyckim ogrodzie muz…", zorganizowanego w roku 2015 przez PAN Bibliotekę Gdańską i Katedrę Filologii Klasycznej Uniwersytetu Gdańskiego. W tomie pierwszym, wydanym w 2016 roku pod tytułem W gdańskim ogrodzie muz, obok podstawowych dla recepcji kultury grecko-rzymskiej sfer ekspresji, jaką jest poezja i sztuka perswazji,...
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Transcriptional activity of epigenetic remodeling genes declines in keratinocytes after in vitro expansion
PublikacjaPURPOSE: In vitro expansion is an invaluable method to obtain keratinocytes in amounts necessary for effective transplantation therapies. In vitro cell culturing provokes questions concerning potential epigenetic alterations occurring in expanded cells in the context of usefulness for transplantation and safety. The purpose of this study was to investigate as to whether keratinocyte expansion is associated with changes in the activity...
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Typing of Scopulariopsis and Microascus fungi by Random Amplified Polymorphic DNA (RAPD)
PublikacjaOBJECTIVES Scopulariopsis species and their teleomorphs of the genus Microascus are commonly isolated from soil, decaying plant material and indoor environments. Moreover, certain Scopulariopsis and Microascus species are recognised as opportunistic human pathogens. Although most species can be identified by detailed morphological study, phenotypic characters appear to overlap in several cases and morphology seems to be insufficient...
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Ogród muz I
PublikacjaNiniejsza książka składa się z dwóch części. W pierwszej znajdują się eseje wprowadzające Czytelnika w tematykę obecności tradycji antycznej w Gdańsku wczesnonowożytnym. Ich autorami są młodzi badacze z różnych ośrodków akademickich. Część druga to katalog wystawy, która miała miejsce w siedzibie Polskiej Akademii Nauk Biblioteki Gdańskiej (19 czerwca do 31 lipca 2015 roku). Na ekspozycji zaprezentowano stare druki i rękopisy...
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W gdańskim ogrodzie muz. Gimnazjum Akademickie w Gdańsku wobec kultury starożytnych Greków i Rzymian: idee, teksty, artefakty.
PublikacjaNiniejsza książka składa się z dwóch części. W pierwszej znajdują się eseje wprowadzające Czytelnika w tematykę obecności tradycji antycznej w Gdańsku wczesnonowożytnym. Ich autorami są młodzi badacze z różnych ośrodków akademickich. Część druga to katalog wystawy, która miała miejsce w siedzibie Polskiej Akademii Nauk Biblioteki Gdańskiej (19 czerwca do 31 lipca 2015 roku). Na ekspozycji zaprezentowano stare druki i rękopisy...
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Diagnostic PCR tests for Microsporum audouinii, M. canis and Trichophyton infections
PublikacjaSince traditional diagnosis of dermatophyte infections is slow, we present a rapid new pcr test for detection of trichophyton spp., microsporum canis and m. audouinii infections. the performance of the test was evaluated with: 58 dermatophyte isolates; 10 yeast, mould and human dna control samples; 25 routine specimens from patients suspected of having dermatophytosis; 10 hair specimens from guinea pigs experimentally infected...
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Биополитика согласно Чеславу Милошу
PublikacjaThe paper shows the origins of the transformation of history into biohistory as well as politics into biopolitics in the writings of Miłosz. It takes into account his view of history when he was a child and his social experiences when he was at school. It proves that the fact that Miłosz comprehends the world within the categories of biopolitics results from projecting Darwinism on the historical past, and then projecting on...
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Architektura a dekonstrukcja. Przypadek Petera Eisenmana i Bernarda Tschumiego
PublikacjaArchitecture and Deconstruction Case of Peter Eisenman and Bernard Tschumi Introduction Towards deconstruction in architecture Intensive relations between philosophical deconstruction and architecture, which were present in the late 1980s and early 1990s, belong to the past and therefore may be described from a greater than...
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A demand for innovation support in samll and medium-sized enterprises in the baltic sea region
Publikacjathe aim of the expertise is to analyze the actual demand of smes from the baltic sea region for innovation support. the results of the conducted study can help formulate recommendations designed to increase innovation and competitiveness of smes in the baltic sea region in the future. research activities of this study include: the evaluation of innovation level of the baltic sea region enterprises (type and intensity of implemented...