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Search results for: FILAGGRIN, EXTRACELLULAR VESICLES, CD1A
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Keratinocyte-derived small extracellular vesicles supply antigens for CD1a-resticted T cells and promote their type 2 bias in the context of filaggrin insufficiency
PublicationIntroduction: Exosome-enriched small extracellular vesicles (sEVs) are nanosized organelles known to participate in long distance communication between cells, including in the skin. Atopic dermatitis (AD) is a chronic inflammatory skin disease for which filaggrin (FLG) gene mutations are the strongest genetic risk factor. Filaggrin insufficiency affects multiple cellular function, but it is unclear if sEV-mediated cellular communication...
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O07 Filaggrin insufficiency renders keratinocyte-derived small extracellular vesicles capable of affecting CD1a-mediated T-cell responses and promoting allergic inflammation
PublicationThe association between FLG loss-of-function mutations and the development of atopic dermatitis (AD) indicates that filaggrin is critical to skin barrier function; the mutations are also linked to additional allergic manifestations, but it is unknown how the skin may influence inflammation in distant tissues. Here we investigated the impact of filaggrin insufficiency on keratinocyte-derived exosome-enriched small extracellular...
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Perspectives and challenges in extracellular vesicles untargeted metabolomics analysis
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MicroRNA composition of plasma extracellular vesicles: a harbinger of late cardiotoxicity of doxorubicin
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Isolation of Citrus lemon extracellular vesicles: Development and process control using capillary electrophoresis
PublicationA new and scalable method for the isolation of extracellular vesicles (EV) from Citrus lemon juice samples was developed. The methodology included preliminary preconcentration of the sample using ultrafiltration (UF) followed by size-exclusion chromatography (SEC) purification and final preconcentration of the eluates. Transmission electron microscopy and proteomic analysis showed that isolates contained exosome-like vesicles, exocyst-positive...
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Nanoparticle Tracking Analysis of Urinary Extracellular Vesicle Proteins as a New Challenge in Laboratory Medicine
PublicationUrinary extracellular vesicle (uEV) proteins may be used as specific markers of kidney damage in various pathophysiological conditions. The nanoparticle-tracking analysis (NTA) appears to be the most useful method for the analysis of uEVs due to its ability to analyze particles below 300 nm. The NTA method has been used to measure the size and concentration of uEVs and also allows for a deeper analysis of uEVs based on their protein...
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Ionizing radiation affects the composition of the proteome of extracellular vesicles released by head-and-neck cancer cells in vitro
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Proteomic profile of melanoma cell‐derived small extracellular vesicles in patients’ plasma: a potential correlate of melanoma progression
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Quality Control of Bacterial Extracellular Vesicles with Total Protein Content Assay, Nanoparticles Tracking Analysis, and Capillary Electrophoresis
PublicationExtracellular vesicles (EVs) were isolated from Pectobacterium zantedeschiae culturing media using direct ultracentrifugation (UC), iodixanol cushion ultracentrifugation (ICUC), and iodixanol density gradient ultracentrifugation (IDGUC) techniques. The isolates were characterized with total protein content assay (bicinchoninic acid assay, BCA), nanoparticles tracking analysis (NTA), and capillary electrophoresis (CE). A satisfactory...
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Induced Pluripotent Stem Cell (iPSC)–Derived Extracellular Vesicles Are Safer and More Effective for Cardiac Repair Than iPSCs
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Direct injection liquid chromatography-mass spectrometry (DI-LC-MS) analysis for rapid lipidomic profiling of extracellular vesicles
PublicationExtracellular vesicles (EVs) are small, spherical particles produced by eukaryotic and prokaryotic cells, surrounded by a bilayer membrane and carrying various bioactive molecules, such as proteins, surface receptors, membrane and soluble proteins, lipids, and nucleic acids. EVs are of substantial interest because of their important roles in cell communication, epigenetic regulation and possible application in disease diagnosis...
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MicroRNA composition of plasma extracellular vesicles: a harbinger of late cardiotoxicity in long-term survivors of acute lymphoblastic leukemia treated with doxorubicin
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Electric Vehicles and Vehicle–Grid Interaction in the Turkish Electricity System
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Extracellular proteolytic activity of Deinococcus geothermalis
PublicationProduction of extracellular protease by extremophilic bacteria Deinococcus geothermalis cultivated in liquid media containing 0.1% (w/v) of peptone K, 0.1% yeast extract and 0.2% marine salt reached a maximum in 14 h of the cell growth at 45°C and pH 8.0. The enzyme was purified by a two-step procedure using fractionation by a graded ammonium sulphate precipitation technique and gel filtration on Sephadex G-100 column. Protease...
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Surface-enhanced Raman scattering (SERS) and tip-enhanced Raman scattering (TERS) in label-free characterization of erythrocyte membranes and extracellular vesicles at the nano-scale and molecular level
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Neutrophil extracellular traps as the main source of eDNA
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Extracellular miRNAs as Biomarkers of Head and Neck Cancer Progression and Metastasis
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Conceptual methods of old-timer vehicles inspection tests based on current technical inspection methods at vehicle inspection stations
PublicationPrzedstawiono koncepcję metodyki okresowych badań technicznych pojazdów zabytkowych w stacjach kontroli pojazdów.
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The Extracellular Bacterial HtrA Proteins as Potential Therapeutic Targets and Vaccine Candidates
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Intelligent Autonomous Vehicles
PublicationAdvanced mobile vehicles and robots have long been one of the main issues in engineering. They have various applications in emergency, lifeguarding and entertainment as well as in various industrial, civil and military systems. Among them, you can distinguish robots that can move in an open environment or operate only in predetermined confined spaces. Open world robots are very demanding because they have...
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Application of CDMA modulated signals in seafloor remote sensing
PublicationW artykule omowiono zastosowanie modulacji CDMA w procesie detekcji echa od dna. Wyniki badan przedstawone zostaly dla symulacji z wykorzystaniem symulatora BORIS.
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Magnetic signatures of land vehicles
PublicationThe results of numerical simulations of the magnetic field surrounding of land vehicles' models have been presented in this paper. Vehicle containing of ferromagnetic elements distorts the Earth's magnetic field. This distortion can be measured by magnetometers. It is possible to detect and identify an object on the basis of its magnetic signature.
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Extracellular Matrix Proteins Expression Profiling in Chemoresistant Variants of the A2780 Ovarian Cancer Cell Line
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Aggregated neutrophil extracellular traps resolve inflammation by proteolysis of cytokines and chemokines and protection from antiproteases
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Certyfikate of roadworthiness for antique veicles
PublicationPrzedstawiono definicję pojazdu zabytkowego oraz zaprezentowano procedurę rejestacji takiego pojazdu. Przeanalizowano warunki dopuszczenia do ruchu pojazdów zabytkowych podając niedoskonałości obecnego systemu. Podano koncepcję nowej metodyki badań technicznych pojazdów zabytkowych mającą na celu poprawę bezpieczeństwa w ruchu drogowym. Przedstawiono zasady pomiaru sił hamowania na stanowisku rolkowym i płytowym.
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Enhanced method of DS-CDMA low level singnals detection
PublicationThe following article comprises three main parts. The first one generally describes two methods of low level signals detection which are in the interest of this study. The signal spectrum averaging technique is shown as well as the method exploiting averaged spectrum of the signal raised to the power of 2n (nN). Additionally, this section briefly presents proposed enhancements and modifications of these two solutions, which allow...
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Theoretical and Measurement Investigations of DS CDMA Signal Detection Problem
PublicationPrzedstawiono opis teoretyczny wybranej motody wykrywania sygnałów z widmem rozproszonym DS CDMA. Przedstawiono prototypowe stanowisko laboratoryjne do wykrywania sygnałów DS CDMA i zaprezentowano wyniki pomiarowe.
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Transmission Quality in MC-CDMA Radio Interface with Turbo Codes
PublicationMulti-Carrier Code Division Multiple Access techiques is a combination of two radio access techniques: Code Division Multiple Access (CDMA) and Orthogonal Frequency-called Division Multiplexing (OFDM) and has the advantages of both techniques. The paper presents the design of transmitter and receiver for MC-CDMA radio interface and the simulation results of the transmission quality in AWGN channel.
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Badanie jakości transmisji w interfejsie radiowym MC-CDMA
PublicationTechnika MC-CDMA (ang. Multi-Carrier Code Division Multiple Access) powstała z połączenia dwóch wcześniej stosowanych technik dostępu radiowego: CDMA (ang. Code Division Multiple Access) i OFDM (ang. Orthogonal Frequency-Division Multiplexing) i posiada zalety obu tych technologii. W referacie przedstawiona została budowa nadajnika i odbiornika dla tego interfejsu radiowego oraz wyniki badań symulacyjnych jakości transmisji w tym...
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The extracellular loop of Man-PTS subunit IID is responsible for the sensitivity of Lactococcus garvieae to garvicins A, B and C
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The effects of NP-POL, the nonapeptide constituent of Colostrinin, on intracellular antioxidant system
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Safety assurance strategies for autonomous vehicles
PublicationAssuring safety of autonomous vehicles requires that the vehicle control system can perceive the situation in the environment and react to actions of other entities. One approach to vehicle safety assurance is based on the assumption that hazardous sequences of events should be identified during hazard analysis and then some means of hazard avoidance and mitigation, like barriers, should be designed and implemented. Another approach...
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Injuries to Users of Single-Track Vehicles
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Sensors and Sensor’s Fusion in Autonomous Vehicles
PublicationAutonomous vehicle navigation has been at the center of several major developments, both in civilian and defense applications. New technologies such as multisensory data fusion, big data processing, and deep learning are changing the quality of areas of applications, improving the sensors and systems used. New ideas such as 3D radar, 3D sonar, LiDAR, and others are based on autonomous vehicle revolutionary development. The Special...
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Changes in Nuclear Gene Expression Related to Mitochondrial Function Affect Extracellular Matrix, Collagens, and Focal Adhesion in Keratoconus
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cDNA sequencing improves the detection of P53 missense mutations in colorectal cancer
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Poprawa rozdzielczości pomiarów TDOA w radionawigacyjnym odbiorniku DS-CDMA
PublicationReferat przedstawia propozycję poprawy rozdzielczości pomiaru różnic czasów nadejścia sygnałów (TDOA) w odbiorniku DS-CDMA do celów radiolokalizacyjnych i radionawigacyjnych przy użyciu pętli śledzenia fazy ciągu rozpraszającego wykorzystującej filtry ułamkowoopóźniające. Opisana jest budowa i funkcjonowanie proponowanego rozwiązania wraz z wynikami badań laboratoryjnych.
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Analizowanie transmisji DS CDMA : badania w środowisku sygnałów rzeczywistych
PublicationW referacie opisano metodę wykrywania sygnałów DS CDMA z modulacją BPSK poprzez badanie widma gęstości mocy (WGM) spotęgowanego sygnału. Na przykładzie eksperymentów przeprowadzonych z użyciem rzeczywistych sygnałów radiokomunikacyjnych pokazano wpływ emisji wąskopasmowych występujących w analizowanym paśmie częstotliwości na efektywność procesu wykrywania. Zaprezentowano wyniki eksperymentu, którego celem było wykrycie sygnałów...
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Zwielokrotnienie kodowe CDMA w szerokopasmowych pasywnych optycznych sieciach dostępowych.
PublicationW wielousługowej sieci dostępowej, wykonanej jako optyczna sieć pasywna(PON) wielodostęp ze zwielokrotnieniem kodowym CDMA wykazuje zalety przy transmisji pakietowej i umożliwią łatwą wymianę pomiędzy liczbą aktywnych użytkowników, szybkością transmisji i jej jakością.
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Emission of CO2 and Fuel Consumption for Automotive Vehicles
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Style Transfer for Detecting Vehicles with Thermal Camera
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Dynamics of Railway Vehicles Movement on Transition Curves
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LOW NOISE TIRES FOR HYBRID AND ELECTRIC VEHICLES
PublicationLow Emission Vehicles that are hybrid and electric cars may benefit from specially designed tires that are optimized for driving conditions typical for such vehicles. It is possible that in the future Low Emission Vehicles, especially passenger cars, will substitute conventional vehicles in all applications, however for the time being they are mostly used in urban and suburban areas. Urban traffic has a rather low demand for grip...
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Travel Time of Public Transport Vehicles Estimation
PublicationEffective prediction of speed is central to advanced traveler information and transportation management systems. The speed of public transport vehicles is affected by many external factors including traffic volume, organization and infrastructure. The literature presents methods for estimating travel time on sections of a transport network and vehicle arrival at stops, often making use of the AVL (automatic vehicle location). The...
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Style Transfer for Detecting Vehicles with Thermal Camera
PublicationIn this work we focus on nighttime vehicle detection for intelligent traffic monitoring from the thermal camera. To train a Convolutional Neural Network (CNN) detector we create a stylized version of COCO (Common Objects in Context) dataset using Style Transfer technique that imitates images obtained from thermal cameras. This new dataset is further used for fine-tuning of the model and as a result detection accuracy on images...
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Gene Expression and Activity Profiling Reveal a Significant Contribution of Exo‐Phosphotransferases to the Extracellular Nucleotides Metabolism in HUVEC Endothelial Cells
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Extracellular biosynthesis of cobalt ferrite nanoparticles by Monascus purpureus and their antioxidant, anticancer and antimicrobial activities: Yield enhancement by gamma irradiation
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Transport of Particles in Intestinal Mucus under Simulated Infant and Adult Physiological Conditions: Impact of Mucus Structure and Extracellular DNA
PublicationThe final boundary between digested food and the cells that take up nutrients in the small intestine is a protective layer of mucus. In this work, the microstructural organization and permeability of the intestinal mucus have been determined under conditions simulating those of infant and adult human small intestines. As a model, we used the mucus from the proximal (jejunal) small intestines of piglets and adult pigs. Confocal...
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Performance analysis of data transmission in MC-CDMA radio interface with turbo codes
PublicationMulti-carrier code division multiple access (MC-CDMA) technique is a combination of two radio access techniques: CDMA and orthogonal frequency division multiplexing and has the advantages of both techniques. The paper presents the design of transmitter and receiver for MC-CDMA radio interface. It also presents encoders and decoders of turbo codes which were used in simulation of the MC-CDMA technique. Two turbo codes with 8-state...
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Sprzętowa realizacja odbiornika sygnałów DS-CDMA w technice radia programowalnego
PublicationRóżnorodność standardów systemów radiokomunikacyjnych, powoduje, że niezbędne jest skonstruowanie wielosystemowego terminala ruchomego, mającego techniczne możliwości współpracy z systemami radiokomunikacyjnymi działającymi w różnych standardach. W ten sposób zrodziła się idea radia definiowanego programowego SDR (Software Defined Radio), bazująca na uniwersalnej warstwie sprzętowej, o funkcjonalności której decyduje jedynie warstwa...