Filters
total: 2787
-
Catalog
displaying 1000 best results Help
Search results for: GENOME EDITING
-
New Gene Markers of Angiogenesis and Blood Vessels Development in Porcine Ovarian Granulosa Cells during Short-Term Primary Culture In Vitro
Publication -
Determination of BRAF V600E (VE1) protein expression and BRAF gene mutation status in codon 600 in borderline and low-grade ovarian cancers
Publication -
Analysis of epigenetic clocks links yoga, sleep, education, reduced meat intake, coffee, and a SOCS2 gene variant to slower epigenetic aging
Publication -
Ambulatory Systolic Blood Pressure is Related to the Deletion Allele of the Angiotensin I Converting Enzyme Gene in Young Normotensives with Parental History of Hypertension
Publication -
Complete tumour regressions induced by vaccination with IL-12 gene-transduced tumour cells in combination with IL-15 in a melanoma model in mice
Publication -
Untargeted Lipidomics Analysis of the Cyanobacterium Synechocystis sp. PCC 6803: Lipid Composition Variation in Response to Alternative Cultivation Setups and to Gene Deletion
PublicationCyanobacteria play an important role in several ecological environments, and they are widely accepted to be the ancestors of chloroplasts in modern plants and green algae. Cyanobacteria have become attractive models for metabolic engineering, with the goal of exploring them as microbial cell factories. However, the study of cyanobacterial lipids’ composition and variation, and the assessment of the lipids’ functional and structural...
-
A novel cold-active β-D-galactosidase with transglycosylation activity from the Antarctic Arthrobacter sp. 32cB - gene cloning, purification and characterization
PublicationA gene encoding a novel β-D-galactosidase from the psychrotolerant Antarctic bacterium Arthrobacter sp. 32cB was isolated, cloned and expressed in Escherichia coli. The active form of recombinant β-D-galactosidase consists of two subunits with a combined molecular weight of approximately 257 kDa. The enzyme's maximum activity towards o-nitrophenyl-β-D-galactopyranoside was determined as occurring at 28 °C and pH 8.0. However, it...
-
Multiple transcriptional factors regulate transcription of the rpoE gene in Escherichia coli under different growth conditions and when the lipopolysaccharide biosynthesis is defective.
PublicationThe RpoE sigma factor is essential for the viability of Escherichia coli. RpoE regulates extracytoplasmic functions including lipopolysaccharide (LPS) translocation and some of its non-stoichiometric modifications. Transcription of the rpoE gene is positively autoregulated by EσE and by unknown mechanisms that control the expression of its distally located promoter(s). Mapping of 5′ ends of rpoE mRNA identified five new transcriptional...
-
ICT in Management - Spring 2024
e-Learning CoursesICT in Management course for Bachelor in Management students (Spring 2024 edition)
-
Audit of the existing surfaces /pavements of sidewalks and roads in the Gdańsk-Oliwa district, with particular emphasis on the location of the "Polanki" market and its direct neighbourhood in the contexts of pavement design of the "Polanki"market; stage from 2019 year.
Open Research DataThe document presents a valorization of paved surfaces (sidewalks and roads) in the Gdańsk-Oliwa district, prapared on the basis of an preliminary inventory work – 21 tables (one table for each street ) with a description of the street and materials used, mostly supplemented with photographic material. The valorization, after the initial inventory,...
-
The usage of the openBIM idea in architectural design on the example of Blender and BlenderBIM add-on
PublicationThe aim of the article is the research about the method and advancement of the openBIM idea implementation, clearly defined by the buildingSMART organization. The conducted analysis are limited to the Blender program and the BlenderBIM add-on and has been embedded in the context of the work of an architect and other IT solutions. The article presents four aspects of the openBIM concept implementation. The first approach relates...
-
Agnieszka Sikora, WCH, I st, 3 sem, 22/23 z
e-Learning CoursesPlease, buy Language Leader Upper-Intermediate from 2015, the 1st edition.
-
Paweł Ziemiański dr
PeoplePaweł Ziemiański - assistant professor at the Faculty of Management and Economics of the Gdańsk University of Technology. His research interests concern entrepreneurship, entrepreneurial intentions, and the dark side of entrepreneurship. He also conducted research regarding psychological aspects of exercising power in organizations. In teaching, he is interested in developing and using case studies. He took part in the training...
-
Personal Branding and Brand Loyalty, Social Network Users Brand Identification: Polish-French Model
PublicationSocial network brand sites are increasingly attracting the attention of scientists and managers intrigued by their potential application for brand value creation. The aim of this research, based on a multinational sample, is to fill the gap in understanding how users choose among social networking sites as an act of brand identification. The authors of the paper point to the fact that creating a personal brand is becoming more...
-
Society 4.0: Issues, Challenges, Approaches, and Enabling Technologies
PublicationThis guest edition of Cybernetics and Systems is a broadening continuation of our last year edition titled “Intelligence Augmentation and Amplification: Approaches, Tools, and Case Studies”. This time we cover research perspective extending towards what is known as Society 4.0. Bob de Vit brought the concept of Society 4.0 to life in his book “Society 4.0 – resolving eight key issues to build a citizens society”. From the Systems...
-
Janusz Datta prof. dr hab. inż.
PeopleIn 1988, Janusz Datta finished his studies at the Gdańsk University of Technology (GUT), Faculty of Chemistry. Since then he has been employed at the Faculty of Chemistry in the Department of Polymer Technology. During the first six years of employement he was worked as a scientist. In this period, he travelled extensively to ZACHEM company located in Bydgoszcz to gain practical experience related to chemical processing. In 1994,...
-
Technical Writing
e-Learning Courses -
Workshop on Graph Theory
EventsThe Gdańsk Workshop on Graph Theory (GWGT) is an annual, informal workshop whose goal is to provide a forum for scientists to meet, present their work, interact, and establish collaborations in the field of Graph Theory
-
Allelic length of a CA dinucleotide repeat in theegfr gene correlates with the frequency of amplifications of this sequence—first results of an inter-ethnic breast cancer study
Publication -
New Gene Markers for Metabolic Processes and Homeostasis in Porcine Buccal Pouch Mucosa during Cells Long Term-Cultivation—A Primary Culture Approach
Publication -
Mosaic Structure of p1658/97, a 125-Kilobase Plasmid Harboring an Active Amplicon with the Extended-Spectrum β-Lactamase Gene bla SHV-5
Publication -
Abscisic Acid Regulates the 3-Hydroxy-3-methylglutaryl CoA Reductase Gene Promoter and Ginsenoside Production in Panax quinquefolium Hairy Root Cultures
Publication -
Relationship between Telomere Length, TERT Genetic Variability and TERT, TP53, SP1, MYC Gene Co-Expression in the Clinicopathological Profile of Breast Cancer
PublicationThe molecular mechanisms of telomerase reverse transcriptase (TERT) upregulation in breast cancer (BC) are complex. We compared genetic variability within TERT and telomere length with the clinical data of patients with BC. Additionally, we assessed the expression of the TERT, MYC, TP53 and SP1 genes in BC patients and in BC organoids (3D cell cultures obtained from breast cancer tissues). We observed the same correlation in the...
-
Assessment of Poland's competitiveness as a location for BPO/SSC projects
Open Research DataIn compiled by A.T. Kearney in subsequent editions of the ranking (2009, 2011, 2016, 2019), the first three positions were taken by the same countries. India received the highest marks with a clear advantage. The country is a world leader in terms of attractiveness for locating business service centers. India can offer English-speaking skilled labor...
-
CANADIAN JOURNAL OF ELECTRICAL AND COMPUTER ENGINEERING-REVUE CANADIENNE DE GENIE ELECTRIQUE ET INFORMATIQUE
Journals -
Digital signal processing applied to the modernization of Polish Navy sonars
PublicationThe article presents the equipment and digital signal processing methods used for modernizing the Polish Navy’s sonars. With the rapid advancement of electronic technologies and digital signal processing methods, electronic systems, including sonars, become obsolete very quickly. In the late 1990s a team of researchers of the Department of Marine Electronics Systems, Faculty of Electronics, Telecommunications and Informatics, Gdansk...
-
Marek Chodnicki dr inż.
People -
Zhongguo Shiyou Daxue Xuebao (Ziran Kexue Ban)/Journal of China University of Petroleum (Edition of Natural Science)
Journals -
Beijing Huagong Daxue Xuebao (Ziran Kexueban)/Journal of Beijing University of Chemical Technology (Natural Science Edition)
Journals -
Xi'an Shiyou Daxue Xuebao (Ziran Kexue Ban)/Journal of Xi'an Shiyou University, Natural Sciences Edition
Journals -
Inserts amplification for knockout cassette construction
Open Research DataThe goal of the study was to obtain optimal conditions for amplification of upstream and downstream genes fragments and introduce a cleavage site for restriction enzymes into them thanks to which it will be possible to clone fragments into a plasmid containing elements of the knockout cassette. The constructed cassettes will enable the removal of the...
-
New Gene Markers Expressed in Porcine Oviductal Epithelial Cells Cultured Primary In Vitro Are Involved in Ontological Groups Representing Physiological Processes of Porcine Oocytes
Publication -
Herbarium of Division of Marine Biology and Ecology University of Gdańsk (DMBE)
Open Research DataHerbarium of Division of Marine Biology and Ecology University of Gdańsk (DMBE) is a research herbarium encompassing specimens of vascular plants and algae hosted by the Laboratory of Marine Plant Ecology at University of Gdańsk, Poland. The aim of Herbarium is to preserve marine plant and algae collections mostly from the Gulf of Gdańsk, but the herbarium...
-
Burnout as a State: Random-Intercept Cross-Lagged Relationship Between Exhaustion and Disengagement in a 10-Day Study [Response to Letter]
PublicationWe are writing in response to the Letter to the Editor by Block, Bair, and Carillo regarding our study, which took a multilevel approach to examine cross-lagged and co-existing relationships between exhaustion and disengagement, measured daily for ten consecutive working days using the Oldenburg Burnout Inventory (OLBI). We assessed the job burnout symptoms using only eight items, all of them worded negatively. Following Block,...
-
Conservation and diversity of MutS proteins
PublicationThe homologues of MutS, mismatch repair protein, exist in all prokaryotes, with the exception of Actinobacteria, Mollicutes and part of the Archaea. Multiple alignments of 316 MutS amino acid sequences from 169 species revealed conserved residues and sequence motifs distinguishing MutS homologues. All MutS homologues show high conservation within the ATPase domain. MutS1, the homologue responsible for DNA mismatch recognition,...
-
Ewolucja chemotaksji organizmów jednokomórkowych w dwuwymiarowym środowisku
PublicationOpracowany przez nas model środowiska oparty jest na fizyce dyfuzji płynów i umożliwia symulację dyfuzji morfogenów. Sztuczne organizmy w tym środowisku wykazują chemotaksję: poruszają się reagując na zmianę stężenia substancji chemicznych. Organizm sterowany jest za pomocą sieci genowej kodowanej w liniowym genomie. Organizmy rozmnażają się przez podział. Przeprowadziliśmy szereg doświadczeń pozwalających na obserwację zachowania...
-
Evolution of Animats Following a Moving Target in an Artificial Ecosystem
PublicationMany biological animals, even microscopically small, are able to track moving sources of food. In this paper, we investigate the emergence of such behavior in artificial animals (animats) in a 2-dimensional simulated liquid environment. These "predators" are controlled by evolving artificial gene regulatory networks encoded in linear genomes. The fate of the predators is determined only by their ability to gather food and reproduce—no...
-
Novel DNA-binding protein from Nanoarchaeum equitans Kin4-M binds all kinds of nucleic acids
PublicationNanoarchaeum equitans is the only known representative of Archaea phylum Nanoarchaeota and stands out as one of the tiniest known living organism. What is more it has smallest genome, which is only 490.885 base pairs long. It is also one of the most compact genomes. According to predictions about 95% of the DNA encodes proteins or stable RNA. Nanoarchaeum equitans lacks genes for most vital metabolic pathways including lipid, cofactor,...
-
Customer Assessment of Brand Valuation and Social Media
PublicationThe research problem engaged in this article is to determine whether contemporary consumers are able to assess brand equity in the overabundance of brands and products with similar features and qualities. The author argues that in the existing circumstances when differences between brands become insignificant the consumer is not capable of assessing their equity adequately. In order to verify the thesis the author has accepted...
-
Gene expression, DNA damage and other stress markers in Sinapis alba L. exposed to heavy metals with special reference to sewage sludge application on contaminated sites
Publication -
High pressure processing, acidic and osmotic stress increased resistance to aminoglycosides and tetracyclines and the frequency of gene transfer among strains from commercial starter and protective cultures
Publication -
Impact of heat shock transcription factor 1 on global gene expression profiles in cells which induce either cytoprotective or pro-apoptotic response following hyperthermia
Publication -
Cation homeostasis and transport related gene markers are differentially expressed in porcine buccal pouch mucosal cells during long-term cells primary culture in vitro
Publication -
Methyl jasmonate as a control factor of the synthase squalene gene promoter and ginsenoside production in American ginseng hairy root cultured in shake flasks and a nutrient sprinkle bioreactor
Publication -
Integrating Statistical and Machine‐Learning Approach for Meta‐Analysis of Bisphenol A‐Exposure Datasets Reveals Effects on Mouse Gene Expression within Pathways of Apoptosis and Cell Survival
PublicationBisphenols are important environmental pollutants that are extensively studied due to different detrimental effects, while the molecular mechanisms behind these effects are less well understood. Like other environmental pollutants, bisphenols are being tested in various experimental models, creating large expression datasets found in open access storage. The meta‐analysis of such datasets is, however, very complicated for various...
-
Characterization of two aminotransferases from Candida albicans
PublicationAminoadipate aminotransferase (AmAA) is an enzyme of α-aminoadipate pathway (AAP) for l-lysine biosynthesis. AmAA may also participated in biosynthesis or degradation of aromatic amino acids and in d-tryptophan based pigment production. The AAP is unique for fungal microorganisms. Enzymes involved in this pathway have specific structures and properties. These features can be used as potential molecular markers. Enzymes catalyzing...
-
Huazhong Keji Daxue Xuebao (Ziran Kexue Ban)/Journal of Huazhong University of Science and Technology (Natural Science Edition)
Journals -
Liaoning Gongcheng Jishu Daxue Xuebao (Ziran Kexue Ban)/Journal of Liaoning Technical University (Natural Science Edition)
Journals -
Crystal structures of the DsbG disulfide isomerase reveal an unstable disulfide
PublicationDsb proteins control the formation and rearrangement of disulfide bonds during the folding of secreted and membrane proteins in bacteria. DsbG, a member of this family, has disulfide bond isomerase and chaperone activity. Here, we present two crystal structures of DsbG at 1.7- and 2.0-Angstrom resolution that are meant to represent the reduced and oxidized forms, respectively. The oxidized structure, however, reveals a mixture...
-
EMQAL 13th edition
e-Learning Courses